Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno

Detalhes bibliográficos
Autor(a) principal: Carmo, Matheus Rodrigues Silva do
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/11022
Resumo: Uma microbiota intestinal equilibrada está associada a um estilo de vida saudável. O consumo de alimentos prebióticos e / ou que contenham microrganismos probióticos pode assegurar o equilíbrio dessa microbiota. Probióticos são microrganismos vivos que, quando ingeridos em quantidades adequadas, conferem benefícios à saúde do hospedeiro. A capacidade de aderir e colonizar o intestino e de inibição de patógenos representam requisitos essenciais para microrganismos serem considerados probióticos. Os lactobacilos fazem parte do grupo de bactérias ácido-lácticas (LAB), possuindo importantes propriedades tecnológicas e probióticas. O objetivo do estudo foi avaliar as propriedades probióticas de cepas de lactobacilos de origem humana por sua capacidade de adesão, colonização e inibição de patógeno. Foram utilizadas 4 cepas de Lacticaseibacillus paracasei, 3 cepas de Lacticaseibacillus rhamnosus e 2 cepas de Limosilactobacillus. fermentum. Avaliou-se a hidrofobicidade da superfície celular das cepas, sua capacidade de aderir às células epiteliais intestinais (Caco-2) e sua capacidade de inibir o patógeno de origem alimentar Salmonella Typhimurium DTA 41 através dos mecanismos de competição, exclusão e desacoplamento. Apesar de apresentar baixo percentual de hidrofobicidade da superfície celular, todas as cepas apresentaram alta capacidade de adesão nas células Caco-2, não havendo diferença significativa entre as cepas isoladas e a cepa probiótica comercial da Christian Hansen L. casei. Tanto as cepas isoladas quanto a comercial foram capazes de inibir os biofilmes de Salmonella, além de reduzir a adesão do patógeno nas células Caco-2. No entanto, a redução da adesão de Salmonella nas células Caco-2 pelos isolados humanos, especialmente L. rhamnosus DTA 73 foi superior em comparação à cepa comercial, que não foi eficaz em excluir ou desacoplar o patógeno. Em superfície plástica a inibição através dos mecanismos de competição e exclusão foi significativamente mais eficaz quando comparada à inibição por desacoplamento para todas as cepas. Os resultados do estudo sugerem que as cepas isoladas possuem alta capacidade de adesão e inibição de patógeno, podendo ser usada não apenas para prevenir, mas também para tratar diarreia já que também apresentam capacidade de desacoplar Salmonella em células Caco-2. Outros testes in vitro e in vivo devem ser realizados para estabelecer as propriedades probióticas das cepas de lactobacilos, além de compreender os mecanismos que as tornam capazes de aderir às células epiteliais intestinais e de inibir patógenos.
id UFRRJ-1_a97fcce20da716ffd7e1dc8e718d184f
oai_identifier_str oai:rima.ufrrj.br:20.500.14407/11022
network_acronym_str UFRRJ-1
network_name_str Repositório Institucional da UFRRJ
repository_id_str
spelling Carmo, Matheus Rodrigues Silva doLuchese, Rosa Helena270.942.270-00http://lattes.cnpq.br/7341531211426066Luchese, Rosa HelenaFerreira, Elisa Helena da RochaGuerra, André Fioravante149.270.917-40http://lattes.cnpq.br/86823409916988982023-12-22T01:45:41Z2023-12-22T01:45:41Z2020-06-16CARMO, Matheus Rodrigues Silva do. Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno. 2020. 45 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2020.https://rima.ufrrj.br/jspui/handle/20.500.14407/11022Uma microbiota intestinal equilibrada está associada a um estilo de vida saudável. O consumo de alimentos prebióticos e / ou que contenham microrganismos probióticos pode assegurar o equilíbrio dessa microbiota. Probióticos são microrganismos vivos que, quando ingeridos em quantidades adequadas, conferem benefícios à saúde do hospedeiro. A capacidade de aderir e colonizar o intestino e de inibição de patógenos representam requisitos essenciais para microrganismos serem considerados probióticos. Os lactobacilos fazem parte do grupo de bactérias ácido-lácticas (LAB), possuindo importantes propriedades tecnológicas e probióticas. O objetivo do estudo foi avaliar as propriedades probióticas de cepas de lactobacilos de origem humana por sua capacidade de adesão, colonização e inibição de patógeno. Foram utilizadas 4 cepas de Lacticaseibacillus paracasei, 3 cepas de Lacticaseibacillus rhamnosus e 2 cepas de Limosilactobacillus. fermentum. Avaliou-se a hidrofobicidade da superfície celular das cepas, sua capacidade de aderir às células epiteliais intestinais (Caco-2) e sua capacidade de inibir o patógeno de origem alimentar Salmonella Typhimurium DTA 41 através dos mecanismos de competição, exclusão e desacoplamento. Apesar de apresentar baixo percentual de hidrofobicidade da superfície celular, todas as cepas apresentaram alta capacidade de adesão nas células Caco-2, não havendo diferença significativa entre as cepas isoladas e a cepa probiótica comercial da Christian Hansen L. casei. Tanto as cepas isoladas quanto a comercial foram capazes de inibir os biofilmes de Salmonella, além de reduzir a adesão do patógeno nas células Caco-2. No entanto, a redução da adesão de Salmonella nas células Caco-2 pelos isolados humanos, especialmente L. rhamnosus DTA 73 foi superior em comparação à cepa comercial, que não foi eficaz em excluir ou desacoplar o patógeno. Em superfície plástica a inibição através dos mecanismos de competição e exclusão foi significativamente mais eficaz quando comparada à inibição por desacoplamento para todas as cepas. Os resultados do estudo sugerem que as cepas isoladas possuem alta capacidade de adesão e inibição de patógeno, podendo ser usada não apenas para prevenir, mas também para tratar diarreia já que também apresentam capacidade de desacoplar Salmonella em células Caco-2. Outros testes in vitro e in vivo devem ser realizados para estabelecer as propriedades probióticas das cepas de lactobacilos, além de compreender os mecanismos que as tornam capazes de aderir às células epiteliais intestinais e de inibir patógenos.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPERJ - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroBalanced intestinal microbiota is associated with a healthy lifestyle and the consumption of prebiotic foods and /or foods that are sources of probiotic microorganisms are important to achieve this goal. Probiotics are live microorganisms that, when ingested in adequate amounts, confer benefits to the host health. Essential requirements to be considered probiotic are the ability to adhere and colonize the intestine and pathogens inhibition. Lactobacilli are part of the group of lactic acid bacteria (LAB), having important technological and probiotic properties. The objective of the study is to evaluate the probiotic properties of lactobacilli strains from human origin by their capacity for adhesion, colonization and pathogen inhibition. The following strains of lactobacilli were used: 4 strains of Lacticaseibacillus paracasei, 3 strains of Lacticaseibacillus rhamnosus and 2 strains of Limosilactobacillus fermentum. The hydrophobicity of the cell surface of the strains was evaluated, their ability to adhere to intestinal epithelial cells (Caco-2 cells) and their capacity to inhibit the foodborne pathogen Salmonella Typhimurium DTA 41 through the mechanisms of competition, exclusion and displacement. Despite presenting low percentage of cell surface hydrophobicity, all strains showed a high adhesion to Caco-2 cells, with no significant difference between isolated strains and Christian Hansen's commercial probiotic L. casei. Both the isolated strains and the commercial strain were able to inhibit Salmonella biofilms, in addition to reducing the pathogen's adhesion to Caco-2 cells. However, the reduction in Salmonella adhesion to Caco-2 cells by human isolates, especially L. rhamnosus DTA 73, was superior compared to the commercial strain, which was not effective in excluding or displace the pathogen. In the plastic surface, inhibition through competition and exclusion mechanisms was significantly more effective when compared to inhibition by displacement for all strains. The results of the study suggest that the isolated strains have a high capacity for adhesion and pathogen inhibition and should be used not only to prevent, but also to treat diarrhea since they also have the ability to displace Salmonella in Caco-2 cells.Further in vitro and in vivo tests must be carried out to establish the probiotic properties of lactobacilli strains, in addition to understanding the mechanisms which make them capable of adhering to intestinal epithelial cells and inhibiting pathogens.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Ciência e Tecnologia de AlimentosUFRRJBrasilInstituto de TecnologiaLactobacilosprobióticosadesãomucosamicrobiotaCaco-2LactobacilliprobioticsadhesionmucousCiência e Tecnologia de AlimentosPropriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógenoProbiotic properties of lactobacilli strains from human origin: adhesion capacity and pathogen inhibitioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisABRAMOV, V. M.; KOSAREV, I. V.; PRIPUTNEVICH, T. V.; MACHULIN, A. V. et al. S-layer protein 2 of Lactobacillus crispatus 2029, its structural and immunomodulatory characteristics and roles in protective potential of the whole bacteria against foodborne pathogens. International Journal of Biological Macromolecules, 150, p. 400-412, 2020/05/01/ 2020. ALIZADEH BEHBAHANI, B.; NOSHAD, M.; FALAH, F. Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15. Microbial Pathogenesis, 136, p. 103677, 2019/11/01/ 2019. ALTONSY, M. O.; ANDREWS, S. C.; TUOHY, K. M. Differential induction of apoptosis in human colonic carcinoma cells (Caco-2) by Atopobium, and commensal, probiotic and enteropathogenic bacteria: Mediation by the mitochondrial pathway. International Journal of Food Microbiology, 137, n. 2, p. 190-203, 2010/02/28/ 2010. ANNUK, H.; HYNES, S. O.; HIRMO, S.; MIKELSAAR, M. et al. Characterisation and differentiation of lactobacilli by lectin typing. Journal of Medical Microbiology, 50, n. 12, p. 1069-1074, 2001. Article. BÄCKHED, F.; DING, H.; WANG, T.; HOOPER, L. V. et al. The gut microbiota as an environmental factor that regulates fat storage. Proceedings of the National Academy of Sciences of the United States of America, 101, n. 44, p. 15718-15723, 2004. BATISTA, A. L. D.; SILVA, R.; CAPPATO, L. P.; FERREIRA, M. V. S. et al. Developing a synbiotic fermented milk using probiotic bacteria and organic green banana flour. Journal of Functional Foods, 38, p. 242-250, 2017/11/01/ 2017. BELGUESMIA, Y.; ALARD, J.; MENDIL, R.; RAVALLEC, R. et al. In vitro probiotic properties of selected lactobacilli and multi-strain consortium on immune function, gut barrier strengthening and gut hormone secretion. Journal of Functional Foods, 57, p. 382-391, 2019/06/01/ 2019. BU, X.-D.; LI, N.; TIAN, X.-Q.; HUANG, P.-L. Caco-2 and LS174T cell lines provide different models for studying mucin expression in colon cancer. Tissue and Cell, 43, n. 3, p. 201-206, 2011/06/01/ 2011. BYRD, J. C.; BRESALIER, R. S. Mucins and mucin binding proteins in colorectal cancer. Cancer and Metastasis Reviews, 23, n. 1-2, p. 77-99, 2004. Review. CANNON, J. P.; LEE, T. A.; BOLANOS, J. T.; DANZIGER, L. H. Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases. Eur J Clin Microbiol Infect Dis, 24, n. 1, p. 31-40, Jan 2005. CASEY, P. G.; GARDINER, G. E.; CASEY, G.; BRADSHAW, B. et al. A Five-Strain Probiotic Combination Reduces Pathogen Shedding and Alleviates Disease Signs in Pigs Challenged with <em>Salmonella enterica</em> Serovar Typhimurium. Applied and Environmental Microbiology, 73, n. 6, p. 1858, 2007. CHEN, P. X.; ZHANG, H.; MARCONE, M. F.; PAULS, K. P. et al. Anti-inflammatory effects of phenolic-rich cranberry bean (Phaseolus vulgaris L.) extracts and enhanced cellular antioxidant enzyme activities in Caco-2 cells. Journal of Functional Foods, 38, p. 675-685, 2017/11/01/ 2017. CHEN, X.; XU, J.; SHUAI, J.; CHEN, J. et al. The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimurium. International Journal of Food Microbiology, 115, n. 3, p. 307-312, 2007/04/20/ 2007. DAVOODABADI, A.; DALLAL, M. M. S.; LASHANI, E.; EBRAHIMI, M. T. Antimicrobial activity of Lactobacillus spp. isolated from fecal flora of healthy breast-fed infants against diarrheagenic Escherichia coli. Jundishapur Journal of Microbiology, 8, n. 12, 2015. Article. DE ANGELIS, M.; GOBBETTI, M. Environmental stress responses in Lactobacillus: A review. 4, n. 1, p. 106-122, 2004. DE ANGELIS, M.; GOBBETTI, M. Lactobacillus SPP.: General Characteristics☆. In: Reference Module in Food Science: Elsevier, 2016. DEL CARMEN, S.; DE MORENO DE LEBLANC, A.; LEVIT, R.; AZEVEDO, V. et al. Anti-cancer effect of lactic acid bacteria expressing antioxidant enzymes or IL-10 in a colorectal cancer mouse model. International Immunopharmacology, 42, p. 122-129, 2017/01/01/ 2017. DENG, K.; CHEN, T.; WU, Q.; XIN, H. et al. In vitro and in vivo examination of anticolonization of pathogens by Lactobacillus paracasei FJ861111.1. Journal Of Dairy Science, 98, n. 10, p. 6759-6766, 2015. DEVI, S. M.; KURREY, N. K.; HALAMI, P. M. In vitro anti-inflammatory activity among probiotic Lactobacillus species isolated from fermented foods. Journal of Functional Foods, 47, p. 19-27, 2018/08/01/ 2018. DI CRISCIO, T.; FRATIANNI, A.; MIGNOGNA, R.; CINQUANTA, L. et al. Production of functional probiotic, prebiotic, and synbiotic ice creams. Journal of Dairy Science, 93, n. 10, p. 4555-4564, 2010/10/01/ 2010. DIAS, C. O.; DOS SANTOS OPUSKI DE ALMEIDA, J.; PINTO, S. S.; DE OLIVEIRA SANTANA, F. C. et al. Development and physico-chemical characterization of microencapsulated bifidobacteria in passion fruit juice: A functional non-dairy product for probiotic delivery. Food Bioscience, 24, p. 26-36, 2018/08/01/ 2018. FAGHFOORI, Z.; POURGHASSEM GARGARI, B.; SABER GHARAMALEKI, A.; BAGHERPOUR, H. et al. Cellular and molecular mechanisms of probiotics effects on colorectal cancer. Journal of Functional Foods, 18, p. 463-472, 2015/10/01/ 2015. FALAH, F.; VASIEE, A.; BEHBAHANI, B. A.; YAZDI, F. T. et al. Evaluation of adherence and anti-infective properties of probiotic Lactobacillus fermentum strain 4-17 against Escherichia coli causing urinary tract infection in humans. Microbial Pathogenesis, 131, p. 246-253, 2019/06/01/ 2019. FAO/WHO. Probiotics in food: health and nutritional properties and guidelines for evaluation. FAO food and nutrition paper, 0254-4725 ; 85., n. Accessed from https://nla.gov.au/nla.cat-vn3788914, 2006. FAYE, T.; TAMBURELLO, A.; VEGARUD, G. E.; SKEIE, S. Survival of lactic acid bacteria from fermented milks in an in vitro digestion model exploiting sequential incubation in human gastric and duodenum juice. Journal of Dairy Science, 95, n. 2, p. 558-566, 2012/02/01/ 2012. FONTANA, L.; BERMUDEZ-BRITO, M.; PLAZA-DIAZ, J.; MUÑOZ-QUEZADA, S. et al. Sources, isolation, characterisation and evaluation of probiotics. British Journal of Nutrition, 109, n. S2, p. S35-S50, 2013. GAGNON, M.; KHEADR, E. E.; LE BLAY, G.; FLISS, I. et al. In vitro inhibition of Escherichia coli O157:H7 by bifidobacterial strains of human origin. International Journal of Food Microbiology, 92, n. 1, p. 69-78, 2004/04/01/ 2004. GANDHI, A.; SHAH, N. P. Effect of salt stress on morphology and membrane composition of Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum, and their adhesion to human intestinal epithelial-like Caco-2 cells. Journal of Dairy Science, 99, n. 4, p. 2594-2605, 2016/04/01/ 2016. GUERRA, A.; JUNIOR, W.; SANTOS, G.; ANDRIGHETTO, C. et al. Lactobacillus paracasei probiotic properties and survivability under stress-induced by processing and storage of ice cream bar or ice-lolly. Ciência Rural, 08/15 2018. HARDY, H.; HARRIS, J.; LYON, E.; BEAL, J. et al. Probiotics, Prebiotics and Immunomodulation of Gut Mucosal Defences: Homeostasis and Immunopathology. Nutrients, 5, n. 6, 2013. HIDALGO, I.; RAUB, T.; BORCHARDT, R. Characterization of the Human Colon Carcinoma Cell Line (Caco-2) as a Model System for Intestinal Epithelial Permeability. Gastroenterology, 96, p. 736-749, 04/01 1989. HOLZAPFEL, W. H.; WOOD, B. J. B. Introduction to the LAB. In: Lactic Acid Bacteria, 2014. p. 1-12. HOSSAIN, M. I.; MIZAN, M. F. R.; ASHRAFUDOULLA, M.; NAHAR, S. et al. Inhibitory effects of probiotic potential lactic acid bacteria isolated from kimchi against Listeria monocytogenes biofilm on lettuce, stainless-steel surfaces, and MBEC™ biofilm device. LWT, 118, p. 108864, 2020/01/01/ 2020. KAMADA, N.; CHEN, G. Y.; INOHARA, N.; NÚÑEZ, G. Control of pathogens and pathobionts by the gut microbiota. Nature Immunology, 14, p. 685, 06/18/online 2013. Review Article. KOLACEK, S.; HOJSAK, I.; BERNI CANANI, R.; GUARINO, A. et al. Commercial Probiotic Products: A Call for Improved Quality Control. A Position Paper by the ESPGHAN Working Group for Probiotics and Prebiotics. Journal of Pediatric Gastroenterology & Nutrition, 65, n. 1, p. 117-124, 2017. KONINKX, J. F. J. G.; TOOTEN, P. C. J.; MALAGO, J. J. Probiotic bacteria induced improvement of the mucosal integrity of enterocyte-like Caco-2 cells after exposure to Salmonella enteritidis 857. Journal of Functional Foods, 2, n. 3, p. 225-234, 2010/07/01/ 2010. LABOISSE, C.; JARRY, A.; BRANKA, J. E.; MERLIN, D. et al. Recent aspects of the regulation of intestinal mucus secretion. Proceedings of the Nutrition Society, 55, n. 1, p. 259-264, 1996. Article. LIÉVIN-LE MOAL, V.; SERVIN, A. L.; COCONNIER-POLTER, M.-H. The increase in mucin exocytosis and the upregulation of MUC genes encoding for membrane-bound mucins induced by the thiol-activated exotoxin listeriolysin O is a host cell defence response that inhibits the cell-entry of Listeria monocytogenes. Cellular Microbiology, 7, n. 7, p. 1035-1048, 2005. LIU, S.-n.; HAN, Y.; ZHOU, Z.-j. Lactic acid bacteria in traditional fermented Chinese foods. Food Research International, 44, n. 3, p. 643-651, 2011/04/01/ 2011. LÓPEZ, P.; GONZÁLEZ-RODRÍGUEZ, I.; SÁNCHEZ, B.; RUAS-MADIEDO, P. et al. Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression. 78, n. 8, p. 2850-2857, 2012. LÓPEZ, P.; GUEIMONDE, M.; MARGOLLES, A.; SUÁREZ, A. Distinct Bifidobacterium strains drive different immune responses in vitro. International Journal of Food Microbiology, 138, n. 1, p. 157-165, 2010/03/31/ 2010. LY, D.; MAYRHOFER, S.; DOMIG, K. J. Significance of traditional fermented foods in the lower Mekong subregion: A focus on lactic acid bacteria. Food Bioscience, 26, p. 113-125, 2018/12/01/ 2018. MADIGAN, M. T.; MARTINKO, J. M. Prokaryotic diversity: the bacteria. Prentice Hall, Upper Saddle River, NJ.: 2006. (Brock: biology of microorganisms. MARTINS, A. A.; SANTOS-JUNIOR, V. A.; FILHO, E. R. T.; SILVA, H. L. A. et al. Probiotic Prato cheese consumption attenuates development of renal calculi in animal model of urolithiasis. Journal of Functional Foods, 49, p. 378-383, 2018/10/01/ 2018. MASHAK, Z. Antimicrobial Activity of Lactobacillus Isolated From Kashk-e Zard and Tarkhineh, Two Iranian Traditional Fermented Foods. 4, n. 2, p. 7-34692, 2016/5/1 %J Int J Enteric Pathog 2016. MATSUO, K.; OTA, H.; AKAMATSU, T.; SUGIYAMA, A. et al. Histochemistry of the surface mucous gel layer of the human colon. Gut, 40, n. 6, p. 782-789, 1997. Article. MENEZES, A. G. T.; MELO, D. d. S.; RAMOS, C. L.; MOREIRA, S. I. et al. Yeasts isolated from Brazilian fermented foods in the protection against infection by pathogenic food bacteria. Microbial Pathogenesis, 140, p. 103969, 2020/03/01/ 2020. MESSAOUDI, S.; MANAI, M.; KERGOURLAY, G.; PRÉVOST, H. et al. Lactobacillus salivarius: Bacteriocin and probiotic activity. Food Microbiology, 36, n. 2, p. 296-304, 2013/12/01/ 2013. MOHANTY, D.; PANDA, S.; KUMAR, S.; RAY, P. In vitro evaluation of adherence and anti-infective property of probiotic Lactobacillus plantarum DM 69 against Salmonella enterica. Microbial Pathogenesis, 126, p. 212-217, 2019/01/01/ 2019. NAMPOOTHIRI, K. M.; BEENA, D. J.; VASANTHAKUMARI, D. S.; ISMAIL, B. Chapter 3 - Health Benefits of Exopolysaccharides in Fermented Foods. In: FRIAS, J.;MARTINEZ-VILLALUENGA, C., et al (Ed.). Fermented Foods in Health and Disease Prevention. Boston: Academic Press, 2017. p. 49-62. NISHIYAMA, K.; SUGIYAMA, M.; MUKAI, T. Adhesion Properties of Lactic Acid Bacteria on Intestinal Mucin. Microorganisms, 4, n. 3, p. 34, 2016. NURAIDA, L. A review: Health promoting lactic acid bacteria in traditional Indonesian fermented foods. Food Science and Human Wellness, 4, n. 2, p. 47-55, 2015/06/01/ 2015. OLEKSY, M.; KLEWICKA, E. Exopolysaccharides produced by Lactobacillus sp.: Biosynthesis and applications. Critical Reviews in Food Science and Nutrition, 58, n. 3, p. 450-462, 2018/02/11 2018. PATSOS, G.; CORFIELD, A. Management of the human mucosal defensive barrier: Evidence for glycan legislation. Biological Chemistry, 390, n. 7, p. 581-590, 2009. Review. PIDUTTI, P.; FEDERICI, F.; BRANDI, J.; MANNA, L. et al. Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by the Lactobacillus salivarius SGL 03. 124, n. 2, p. 398-407, 2018. PINTO, S. S.; FRITZEN-FREIRE, C. B.; DIAS, C. O.; AMBONI, R. D. M. C. A potential technological application of probiotic microcapsules in lactose-free Greek-style yoghurt. International Dairy Journal, 97, p. 131-138, 2019/10/01/ 2019. RAMA, G. R.; KUHN, D.; BEUX, S.; MACIEL, M. J. et al. Potential applications of dairy whey for the production of lactic acid bacteria cultures. International Dairy Journal, 98, p. 25-37, 2019/11/01/ 2019. RAMIAH, K.; VAN REENEN, C. A.; DICKS, L. M. T. Surface-bound proteins of Lactobacillus plantarum 423 that contribute to adhesion of Caco-2 cells and their role in competitive exclusion and displacement of Clostridium sporogenes and Enterococcus faecalis. Research in Microbiology, 159, n. 6, p. 470-475, 2008/07/01/ 2008. RESTA-LENERT, S.; BARRETT, K. E. Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive <em>Escherichia coli</em> (EIEC). 52, n. 7, p. 988-997, 2003. RINKINEN, M.; WESTERMARCK, E.; SALMINEN, S.; OUWEHAND, A. C. Absence of host specificity for in vitro adhesion of probiotic lactic acid bacteria to intestinal mucus. Veterinary Microbiology, 97, n. 1, p. 55-61, 2003/12/02/ 2003. ROUND, J. L.; MAZMANIAN, S. K. Inducible Foxp3+ regulatory T-cell development by a commensal bacterium of the intestinal microbiota. Proceedings of the National Academy of Sciences, 107, n. 27, p. 12204-12209, 2010. RUIZ-MOYANO, S.; GONÇALVES DOS SANTOS, M. T. P.; GALVÁN, A. I.; MERCHÁN, A. V. et al. Screening of autochthonous lactic acid bacteria strains from artisanal soft cheese: probiotic characteristics and prebiotic metabolism. LWT, 114, p. 108388, 2019/11/01/ 2019. SÁNCHEZ-TAPIA, M.; TOVAR, A. R.; TORRES, N. Diet as Regulator of Gut Microbiota and its Role in Health and Disease. Archives of Medical Research, 50, n. 5, p. 259-268, 2019/07/01/ 2019. SARIKAYA, H.; ASLIM, B.; YUKSEKDAG, Z. Assessment of anti-biofilm activity and bifidogenic growth stimulator (BGS) effect of lyophilized exopolysaccharides (l-EPSs) from Lactobacilli strains. International Journal of Food Properties, 20, n. 2, p. 362-371, 2017/02/01 2017. SERVIN, A. L. Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. 28, n. 4, p. 405-440, 2004. SHINDE, T.; VEMURI, R.; SHASTRI, M. D.; PERERA, A. P. et al. Probiotic Bacillus coagulans MTCC 5856 spores exhibit excellent in-vitro functional efficacy in simulated gastric survival, mucosal adhesion and immunomodulation. Journal of Functional Foods, 52, p. 100-108, 2019/01/01/ 2019. SLOVER, C. M.; DANZIGER, L. Lactobacillus: a Review. Clinical Microbiology Newsletter, 30, n. 4, p. 23-27, 2008/02/15/ 2008. TARRAH, A.; DA SILVA DUARTE, V.; DE CASTILHOS, J.; PAKROO, S. et al. Probiotic potential and biofilm inhibitory activity of Lactobacillus casei group strains isolated from infant feces. Journal of Functional Foods, 54, p. 489-497, 2019/03/01/ 2019. TASNIM, N.; ABULIZI, N.; PITHER, J.; HART, M. M. et al. Linking the Gut Microbial Ecosystem with the Environment: Does Gut Health Depend on Where We Live? Frontiers in Microbiology, 8, n. 1935, 2017-October-06 2017. Perspective. TODOROV, S. D.; BOTES, M.; GUIGAS, C.; SCHILLINGER, U. et al. Boza, a natural source of probiotic lactic acid bacteria. Journal of Applied Microbiology, 104, n. 2, p. 465-477, 2008/02/01 2008. TUO, Y.; SONG, X.; SONG, Y.; LIU, W. et al. Screening probiotics from Lactobacillus strains according to their abilities to inhibit pathogen adhesion and induction of pro-inflammatory cytokine IL-8. Journal of Dairy Science, 101, n. 6, p. 4822-4829, 2018/06/01/ 2018. VASCONCELOS, F. M.; SILVA, H. L. A.; POSO, S. M. V.; BARROSO, M. V. et al. Probiotic Prato cheese attenuates cigarette smoke-induced injuries in mice. Food Research International, 123, p. 697-703, 2019/09/01/ 2019. VASIEE, A.; MORTAZAVI, S. A.; SANKIAN, M.; YAZDI, F. T. et al. Antagonistic activity of recombinant Lactococcus lactis NZ1330 on the adhesion properties of Escherichia coli causing urinary tract infection. Microbial Pathogenesis, 133, p. 103547, 2019/08/01/ 2019. VIEIRA, M. A.; GUERRA, A. F.; LUCHESE, R. H. Mechanisms Of Pathogens Biofilm Reduction By Probiotic Bacteria. Revista Higiene Alimentar, Edição Especial, 2015. WANG, B.; LI, J.; CHEN, J.; HUANG, Q. et al. Effect of live Lactobacillus plantarum L2 on TNF-α-induced MCP-1 production in Caco-2 cells. International Journal of Food Microbiology, 142, n. 1, p. 237-241, 2010/08/15/ 2010. WANG, J.; ZHANG, H.; DU, H.; WANG, F. et al. Identification and characterization of Diutina rugosa SD-17 for potential use as a probiotic. LWT, 109, p. 283-288, 2019/07/01/ 2019. WOLTERS, M.; AHRENS, J.; ROMANÍ-PÉREZ, M.; WATKINS, C. et al. Dietary fat, the gut microbiota, and metabolic health – A systematic review conducted within the MyNewGut project. Clinical Nutrition, 38, n. 6, p. 2504-2520, 2019/12/01/ 2019. WU, M.-H.; PAN, T.-M.; WU, Y.-J.; CHANG, S.-J. et al. Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties. International Journal of Food Microbiology, 144, n. 1, p. 104-110, 2010/11/15/ 2010. XU, X.; BAO, Y.; WU, B.; LAO, F. et al. Chemical analysis and flavor properties of blended orange, carrot, apple and Chinese jujube juice fermented by selenium-enriched probiotics. Food Chemistry, 289, p. 250-258, 2019/08/15/ 2019. XU, Y.; ZHOU, T.; TANG, H.; LI, X. et al. Probiotic potential and amylolytic properties of lactic acid bacteria isolated from Chinese fermented cereal foods. Food Control, 111, p. 107057, 2020/05/01/ 2020. ZHANG, W.; WANG, H.; LIU, J.; ZHAO, Y. et al. Adhesive ability means inhibition activities for lactobacillus against pathogens and S-layer protein plays an important role in adhesion. Anaerobe, 22, p. 97-103, 2013/08/01/ 2013. ZHANG, Y.-C.; ZHANG, L.-W.; TUO, Y.-F.; GUO, C.-F. et al. Inhibition of Shigella sonnei adherence to HT-29 cells by lactobacilli from Chinese fermented food and preliminary characterization of S-layer protein involvement. Research in Microbiology, 161, n. 8, p. 667-672, 2010/10/01/ 2010. ZHANG, Z.; TAO, X.; SHAH, N. P.; WEI, H. Antagonistics against pathogenic Bacillus cereus in milk fermentation by Lactobacillus plantarum ZDY2013 and its anti-adhesion effect on Caco-2 cells against pathogens. Journal of Dairy Science, 99, n. 4, p. 2666-2674, 2016/04/01/ 2016. ZHENG, J.; WITTOUCK, S.; SALVETTI, E.; FRANZ, C. et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. International Journal of Systematic and Evolutionary Microbiology, 70, 04/15 2020.https://tede.ufrrj.br/retrieve/72023/2020%20-%20Matheus%20Rodrigues%20Silva%20do%20Carmo.pdf.jpghttps://tede.ufrrj.br/jspui/handle/jspui/6293Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2023-01-30T18:52:43Z No. of bitstreams: 1 2020 - Matheus Rodrigues Silva do Carmo.pdf: 1972522 bytes, checksum: ad00c59b497b3faf56ab2fda9d9889e9 (MD5)Made available in DSpace on 2023-01-30T18:52:43Z (GMT). No. of bitstreams: 1 2020 - Matheus Rodrigues Silva do Carmo.pdf: 1972522 bytes, checksum: ad00c59b497b3faf56ab2fda9d9889e9 (MD5) Previous issue date: 2020-06-16info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2020 - Matheus Rodrigues Silva do Carmo.pdf.jpgGenerated Thumbnailimage/jpeg1943https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/1/2020%20-%20Matheus%20Rodrigues%20Silva%20do%20Carmo.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD51TEXT2020 - Matheus Rodrigues Silva do Carmo.pdf.txtExtracted Texttext/plain96539https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/2/2020%20-%20Matheus%20Rodrigues%20Silva%20do%20Carmo.pdf.txta97f883ff38e9868d6672d016da57fe0MD52ORIGINAL2020 - Matheus Rodrigues Silva do Carmo.pdfapplication/pdf1972522https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/3/2020%20-%20Matheus%20Rodrigues%20Silva%20do%20Carmo.pdfad00c59b497b3faf56ab2fda9d9889e9MD53LICENSElicense.txttext/plain2089https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/4/license.txt7b5ba3d2445355f386edab96125d42b7MD5420.500.14407/110222023-12-21 22:45:41.571oai:rima.ufrrj.br:20.500.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Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2023-12-22T01:45:41Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false
dc.title.por.fl_str_mv Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
dc.title.alternative.eng.fl_str_mv Probiotic properties of lactobacilli strains from human origin: adhesion capacity and pathogen inhibition
title Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
spellingShingle Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
Carmo, Matheus Rodrigues Silva do
Lactobacilos
probióticos
adesão
mucosa
microbiota
Caco-2
Lactobacilli
probiotics
adhesion
mucous
Ciência e Tecnologia de Alimentos
title_short Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
title_full Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
title_fullStr Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
title_full_unstemmed Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
title_sort Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno
author Carmo, Matheus Rodrigues Silva do
author_facet Carmo, Matheus Rodrigues Silva do
author_role author
dc.contributor.author.fl_str_mv Carmo, Matheus Rodrigues Silva do
dc.contributor.advisor1.fl_str_mv Luchese, Rosa Helena
dc.contributor.advisor1ID.fl_str_mv 270.942.270-00
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7341531211426066
dc.contributor.referee1.fl_str_mv Luchese, Rosa Helena
dc.contributor.referee2.fl_str_mv Ferreira, Elisa Helena da Rocha
dc.contributor.referee3.fl_str_mv Guerra, André Fioravante
dc.contributor.authorID.fl_str_mv 149.270.917-40
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8682340991698898
contributor_str_mv Luchese, Rosa Helena
Luchese, Rosa Helena
Ferreira, Elisa Helena da Rocha
Guerra, André Fioravante
dc.subject.por.fl_str_mv Lactobacilos
probióticos
adesão
mucosa
microbiota
Caco-2
topic Lactobacilos
probióticos
adesão
mucosa
microbiota
Caco-2
Lactobacilli
probiotics
adhesion
mucous
Ciência e Tecnologia de Alimentos
dc.subject.eng.fl_str_mv Lactobacilli
probiotics
adhesion
mucous
dc.subject.cnpq.fl_str_mv Ciência e Tecnologia de Alimentos
description Uma microbiota intestinal equilibrada está associada a um estilo de vida saudável. O consumo de alimentos prebióticos e / ou que contenham microrganismos probióticos pode assegurar o equilíbrio dessa microbiota. Probióticos são microrganismos vivos que, quando ingeridos em quantidades adequadas, conferem benefícios à saúde do hospedeiro. A capacidade de aderir e colonizar o intestino e de inibição de patógenos representam requisitos essenciais para microrganismos serem considerados probióticos. Os lactobacilos fazem parte do grupo de bactérias ácido-lácticas (LAB), possuindo importantes propriedades tecnológicas e probióticas. O objetivo do estudo foi avaliar as propriedades probióticas de cepas de lactobacilos de origem humana por sua capacidade de adesão, colonização e inibição de patógeno. Foram utilizadas 4 cepas de Lacticaseibacillus paracasei, 3 cepas de Lacticaseibacillus rhamnosus e 2 cepas de Limosilactobacillus. fermentum. Avaliou-se a hidrofobicidade da superfície celular das cepas, sua capacidade de aderir às células epiteliais intestinais (Caco-2) e sua capacidade de inibir o patógeno de origem alimentar Salmonella Typhimurium DTA 41 através dos mecanismos de competição, exclusão e desacoplamento. Apesar de apresentar baixo percentual de hidrofobicidade da superfície celular, todas as cepas apresentaram alta capacidade de adesão nas células Caco-2, não havendo diferença significativa entre as cepas isoladas e a cepa probiótica comercial da Christian Hansen L. casei. Tanto as cepas isoladas quanto a comercial foram capazes de inibir os biofilmes de Salmonella, além de reduzir a adesão do patógeno nas células Caco-2. No entanto, a redução da adesão de Salmonella nas células Caco-2 pelos isolados humanos, especialmente L. rhamnosus DTA 73 foi superior em comparação à cepa comercial, que não foi eficaz em excluir ou desacoplar o patógeno. Em superfície plástica a inibição através dos mecanismos de competição e exclusão foi significativamente mais eficaz quando comparada à inibição por desacoplamento para todas as cepas. Os resultados do estudo sugerem que as cepas isoladas possuem alta capacidade de adesão e inibição de patógeno, podendo ser usada não apenas para prevenir, mas também para tratar diarreia já que também apresentam capacidade de desacoplar Salmonella em células Caco-2. Outros testes in vitro e in vivo devem ser realizados para estabelecer as propriedades probióticas das cepas de lactobacilos, além de compreender os mecanismos que as tornam capazes de aderir às células epiteliais intestinais e de inibir patógenos.
publishDate 2020
dc.date.issued.fl_str_mv 2020-06-16
dc.date.accessioned.fl_str_mv 2023-12-22T01:45:41Z
dc.date.available.fl_str_mv 2023-12-22T01:45:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARMO, Matheus Rodrigues Silva do. Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno. 2020. 45 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2020.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/11022
identifier_str_mv CARMO, Matheus Rodrigues Silva do. Propriedades probióticas de lactobacilos isolados de origem humana: capacidade de adesão e inibição de patógeno. 2020. 45 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2020.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/11022
dc.language.iso.fl_str_mv por
language por
dc.relation.references.por.fl_str_mv ABRAMOV, V. M.; KOSAREV, I. V.; PRIPUTNEVICH, T. V.; MACHULIN, A. V. et al. S-layer protein 2 of Lactobacillus crispatus 2029, its structural and immunomodulatory characteristics and roles in protective potential of the whole bacteria against foodborne pathogens. International Journal of Biological Macromolecules, 150, p. 400-412, 2020/05/01/ 2020. ALIZADEH BEHBAHANI, B.; NOSHAD, M.; FALAH, F. Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15. Microbial Pathogenesis, 136, p. 103677, 2019/11/01/ 2019. ALTONSY, M. O.; ANDREWS, S. C.; TUOHY, K. M. Differential induction of apoptosis in human colonic carcinoma cells (Caco-2) by Atopobium, and commensal, probiotic and enteropathogenic bacteria: Mediation by the mitochondrial pathway. International Journal of Food Microbiology, 137, n. 2, p. 190-203, 2010/02/28/ 2010. ANNUK, H.; HYNES, S. O.; HIRMO, S.; MIKELSAAR, M. et al. Characterisation and differentiation of lactobacilli by lectin typing. Journal of Medical Microbiology, 50, n. 12, p. 1069-1074, 2001. Article. BÄCKHED, F.; DING, H.; WANG, T.; HOOPER, L. V. et al. The gut microbiota as an environmental factor that regulates fat storage. Proceedings of the National Academy of Sciences of the United States of America, 101, n. 44, p. 15718-15723, 2004. BATISTA, A. L. D.; SILVA, R.; CAPPATO, L. P.; FERREIRA, M. V. S. et al. Developing a synbiotic fermented milk using probiotic bacteria and organic green banana flour. Journal of Functional Foods, 38, p. 242-250, 2017/11/01/ 2017. BELGUESMIA, Y.; ALARD, J.; MENDIL, R.; RAVALLEC, R. et al. In vitro probiotic properties of selected lactobacilli and multi-strain consortium on immune function, gut barrier strengthening and gut hormone secretion. Journal of Functional Foods, 57, p. 382-391, 2019/06/01/ 2019. BU, X.-D.; LI, N.; TIAN, X.-Q.; HUANG, P.-L. Caco-2 and LS174T cell lines provide different models for studying mucin expression in colon cancer. Tissue and Cell, 43, n. 3, p. 201-206, 2011/06/01/ 2011. BYRD, J. C.; BRESALIER, R. S. Mucins and mucin binding proteins in colorectal cancer. Cancer and Metastasis Reviews, 23, n. 1-2, p. 77-99, 2004. Review. CANNON, J. P.; LEE, T. A.; BOLANOS, J. T.; DANZIGER, L. H. Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases. Eur J Clin Microbiol Infect Dis, 24, n. 1, p. 31-40, Jan 2005. CASEY, P. G.; GARDINER, G. E.; CASEY, G.; BRADSHAW, B. et al. A Five-Strain Probiotic Combination Reduces Pathogen Shedding and Alleviates Disease Signs in Pigs Challenged with <em>Salmonella enterica</em> Serovar Typhimurium. Applied and Environmental Microbiology, 73, n. 6, p. 1858, 2007. CHEN, P. X.; ZHANG, H.; MARCONE, M. F.; PAULS, K. P. et al. Anti-inflammatory effects of phenolic-rich cranberry bean (Phaseolus vulgaris L.) extracts and enhanced cellular antioxidant enzyme activities in Caco-2 cells. Journal of Functional Foods, 38, p. 675-685, 2017/11/01/ 2017. CHEN, X.; XU, J.; SHUAI, J.; CHEN, J. et al. The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella typhimurium. International Journal of Food Microbiology, 115, n. 3, p. 307-312, 2007/04/20/ 2007. DAVOODABADI, A.; DALLAL, M. M. S.; LASHANI, E.; EBRAHIMI, M. T. Antimicrobial activity of Lactobacillus spp. isolated from fecal flora of healthy breast-fed infants against diarrheagenic Escherichia coli. Jundishapur Journal of Microbiology, 8, n. 12, 2015. Article. DE ANGELIS, M.; GOBBETTI, M. Environmental stress responses in Lactobacillus: A review. 4, n. 1, p. 106-122, 2004. DE ANGELIS, M.; GOBBETTI, M. Lactobacillus SPP.: General Characteristics☆. In: Reference Module in Food Science: Elsevier, 2016. DEL CARMEN, S.; DE MORENO DE LEBLANC, A.; LEVIT, R.; AZEVEDO, V. et al. Anti-cancer effect of lactic acid bacteria expressing antioxidant enzymes or IL-10 in a colorectal cancer mouse model. International Immunopharmacology, 42, p. 122-129, 2017/01/01/ 2017. DENG, K.; CHEN, T.; WU, Q.; XIN, H. et al. In vitro and in vivo examination of anticolonization of pathogens by Lactobacillus paracasei FJ861111.1. Journal Of Dairy Science, 98, n. 10, p. 6759-6766, 2015. DEVI, S. M.; KURREY, N. K.; HALAMI, P. M. In vitro anti-inflammatory activity among probiotic Lactobacillus species isolated from fermented foods. Journal of Functional Foods, 47, p. 19-27, 2018/08/01/ 2018. DI CRISCIO, T.; FRATIANNI, A.; MIGNOGNA, R.; CINQUANTA, L. et al. Production of functional probiotic, prebiotic, and synbiotic ice creams. Journal of Dairy Science, 93, n. 10, p. 4555-4564, 2010/10/01/ 2010. DIAS, C. O.; DOS SANTOS OPUSKI DE ALMEIDA, J.; PINTO, S. S.; DE OLIVEIRA SANTANA, F. C. et al. Development and physico-chemical characterization of microencapsulated bifidobacteria in passion fruit juice: A functional non-dairy product for probiotic delivery. Food Bioscience, 24, p. 26-36, 2018/08/01/ 2018. FAGHFOORI, Z.; POURGHASSEM GARGARI, B.; SABER GHARAMALEKI, A.; BAGHERPOUR, H. et al. Cellular and molecular mechanisms of probiotics effects on colorectal cancer. Journal of Functional Foods, 18, p. 463-472, 2015/10/01/ 2015. FALAH, F.; VASIEE, A.; BEHBAHANI, B. A.; YAZDI, F. T. et al. Evaluation of adherence and anti-infective properties of probiotic Lactobacillus fermentum strain 4-17 against Escherichia coli causing urinary tract infection in humans. Microbial Pathogenesis, 131, p. 246-253, 2019/06/01/ 2019. FAO/WHO. Probiotics in food: health and nutritional properties and guidelines for evaluation. FAO food and nutrition paper, 0254-4725 ; 85., n. Accessed from https://nla.gov.au/nla.cat-vn3788914, 2006. FAYE, T.; TAMBURELLO, A.; VEGARUD, G. E.; SKEIE, S. Survival of lactic acid bacteria from fermented milks in an in vitro digestion model exploiting sequential incubation in human gastric and duodenum juice. Journal of Dairy Science, 95, n. 2, p. 558-566, 2012/02/01/ 2012. FONTANA, L.; BERMUDEZ-BRITO, M.; PLAZA-DIAZ, J.; MUÑOZ-QUEZADA, S. et al. Sources, isolation, characterisation and evaluation of probiotics. British Journal of Nutrition, 109, n. S2, p. S35-S50, 2013. GAGNON, M.; KHEADR, E. E.; LE BLAY, G.; FLISS, I. et al. In vitro inhibition of Escherichia coli O157:H7 by bifidobacterial strains of human origin. International Journal of Food Microbiology, 92, n. 1, p. 69-78, 2004/04/01/ 2004. GANDHI, A.; SHAH, N. P. Effect of salt stress on morphology and membrane composition of Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum, and their adhesion to human intestinal epithelial-like Caco-2 cells. Journal of Dairy Science, 99, n. 4, p. 2594-2605, 2016/04/01/ 2016. GUERRA, A.; JUNIOR, W.; SANTOS, G.; ANDRIGHETTO, C. et al. Lactobacillus paracasei probiotic properties and survivability under stress-induced by processing and storage of ice cream bar or ice-lolly. Ciência Rural, 08/15 2018. HARDY, H.; HARRIS, J.; LYON, E.; BEAL, J. et al. Probiotics, Prebiotics and Immunomodulation of Gut Mucosal Defences: Homeostasis and Immunopathology. Nutrients, 5, n. 6, 2013. HIDALGO, I.; RAUB, T.; BORCHARDT, R. Characterization of the Human Colon Carcinoma Cell Line (Caco-2) as a Model System for Intestinal Epithelial Permeability. Gastroenterology, 96, p. 736-749, 04/01 1989. HOLZAPFEL, W. H.; WOOD, B. J. B. Introduction to the LAB. In: Lactic Acid Bacteria, 2014. p. 1-12. HOSSAIN, M. I.; MIZAN, M. F. R.; ASHRAFUDOULLA, M.; NAHAR, S. et al. Inhibitory effects of probiotic potential lactic acid bacteria isolated from kimchi against Listeria monocytogenes biofilm on lettuce, stainless-steel surfaces, and MBEC™ biofilm device. LWT, 118, p. 108864, 2020/01/01/ 2020. KAMADA, N.; CHEN, G. Y.; INOHARA, N.; NÚÑEZ, G. Control of pathogens and pathobionts by the gut microbiota. Nature Immunology, 14, p. 685, 06/18/online 2013. Review Article. KOLACEK, S.; HOJSAK, I.; BERNI CANANI, R.; GUARINO, A. et al. Commercial Probiotic Products: A Call for Improved Quality Control. A Position Paper by the ESPGHAN Working Group for Probiotics and Prebiotics. Journal of Pediatric Gastroenterology & Nutrition, 65, n. 1, p. 117-124, 2017. KONINKX, J. F. J. G.; TOOTEN, P. C. J.; MALAGO, J. J. Probiotic bacteria induced improvement of the mucosal integrity of enterocyte-like Caco-2 cells after exposure to Salmonella enteritidis 857. Journal of Functional Foods, 2, n. 3, p. 225-234, 2010/07/01/ 2010. LABOISSE, C.; JARRY, A.; BRANKA, J. E.; MERLIN, D. et al. Recent aspects of the regulation of intestinal mucus secretion. Proceedings of the Nutrition Society, 55, n. 1, p. 259-264, 1996. Article. LIÉVIN-LE MOAL, V.; SERVIN, A. L.; COCONNIER-POLTER, M.-H. The increase in mucin exocytosis and the upregulation of MUC genes encoding for membrane-bound mucins induced by the thiol-activated exotoxin listeriolysin O is a host cell defence response that inhibits the cell-entry of Listeria monocytogenes. Cellular Microbiology, 7, n. 7, p. 1035-1048, 2005. LIU, S.-n.; HAN, Y.; ZHOU, Z.-j. Lactic acid bacteria in traditional fermented Chinese foods. Food Research International, 44, n. 3, p. 643-651, 2011/04/01/ 2011. LÓPEZ, P.; GONZÁLEZ-RODRÍGUEZ, I.; SÁNCHEZ, B.; RUAS-MADIEDO, P. et al. Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression. 78, n. 8, p. 2850-2857, 2012. LÓPEZ, P.; GUEIMONDE, M.; MARGOLLES, A.; SUÁREZ, A. Distinct Bifidobacterium strains drive different immune responses in vitro. International Journal of Food Microbiology, 138, n. 1, p. 157-165, 2010/03/31/ 2010. LY, D.; MAYRHOFER, S.; DOMIG, K. J. Significance of traditional fermented foods in the lower Mekong subregion: A focus on lactic acid bacteria. Food Bioscience, 26, p. 113-125, 2018/12/01/ 2018. MADIGAN, M. T.; MARTINKO, J. M. Prokaryotic diversity: the bacteria. Prentice Hall, Upper Saddle River, NJ.: 2006. (Brock: biology of microorganisms. MARTINS, A. A.; SANTOS-JUNIOR, V. A.; FILHO, E. R. T.; SILVA, H. L. A. et al. Probiotic Prato cheese consumption attenuates development of renal calculi in animal model of urolithiasis. Journal of Functional Foods, 49, p. 378-383, 2018/10/01/ 2018. MASHAK, Z. Antimicrobial Activity of Lactobacillus Isolated From Kashk-e Zard and Tarkhineh, Two Iranian Traditional Fermented Foods. 4, n. 2, p. 7-34692, 2016/5/1 %J Int J Enteric Pathog 2016. MATSUO, K.; OTA, H.; AKAMATSU, T.; SUGIYAMA, A. et al. Histochemistry of the surface mucous gel layer of the human colon. Gut, 40, n. 6, p. 782-789, 1997. Article. MENEZES, A. G. T.; MELO, D. d. S.; RAMOS, C. L.; MOREIRA, S. I. et al. Yeasts isolated from Brazilian fermented foods in the protection against infection by pathogenic food bacteria. Microbial Pathogenesis, 140, p. 103969, 2020/03/01/ 2020. MESSAOUDI, S.; MANAI, M.; KERGOURLAY, G.; PRÉVOST, H. et al. Lactobacillus salivarius: Bacteriocin and probiotic activity. Food Microbiology, 36, n. 2, p. 296-304, 2013/12/01/ 2013. MOHANTY, D.; PANDA, S.; KUMAR, S.; RAY, P. In vitro evaluation of adherence and anti-infective property of probiotic Lactobacillus plantarum DM 69 against Salmonella enterica. Microbial Pathogenesis, 126, p. 212-217, 2019/01/01/ 2019. NAMPOOTHIRI, K. M.; BEENA, D. J.; VASANTHAKUMARI, D. S.; ISMAIL, B. Chapter 3 - Health Benefits of Exopolysaccharides in Fermented Foods. In: FRIAS, J.;MARTINEZ-VILLALUENGA, C., et al (Ed.). Fermented Foods in Health and Disease Prevention. Boston: Academic Press, 2017. p. 49-62. NISHIYAMA, K.; SUGIYAMA, M.; MUKAI, T. Adhesion Properties of Lactic Acid Bacteria on Intestinal Mucin. Microorganisms, 4, n. 3, p. 34, 2016. NURAIDA, L. A review: Health promoting lactic acid bacteria in traditional Indonesian fermented foods. Food Science and Human Wellness, 4, n. 2, p. 47-55, 2015/06/01/ 2015. OLEKSY, M.; KLEWICKA, E. Exopolysaccharides produced by Lactobacillus sp.: Biosynthesis and applications. Critical Reviews in Food Science and Nutrition, 58, n. 3, p. 450-462, 2018/02/11 2018. PATSOS, G.; CORFIELD, A. Management of the human mucosal defensive barrier: Evidence for glycan legislation. Biological Chemistry, 390, n. 7, p. 581-590, 2009. Review. PIDUTTI, P.; FEDERICI, F.; BRANDI, J.; MANNA, L. et al. Purification and characterization of ribosomal proteins L27 and L30 having antimicrobial activity produced by the Lactobacillus salivarius SGL 03. 124, n. 2, p. 398-407, 2018. PINTO, S. S.; FRITZEN-FREIRE, C. B.; DIAS, C. O.; AMBONI, R. D. M. C. A potential technological application of probiotic microcapsules in lactose-free Greek-style yoghurt. International Dairy Journal, 97, p. 131-138, 2019/10/01/ 2019. RAMA, G. R.; KUHN, D.; BEUX, S.; MACIEL, M. J. et al. Potential applications of dairy whey for the production of lactic acid bacteria cultures. International Dairy Journal, 98, p. 25-37, 2019/11/01/ 2019. RAMIAH, K.; VAN REENEN, C. A.; DICKS, L. M. T. Surface-bound proteins of Lactobacillus plantarum 423 that contribute to adhesion of Caco-2 cells and their role in competitive exclusion and displacement of Clostridium sporogenes and Enterococcus faecalis. Research in Microbiology, 159, n. 6, p. 470-475, 2008/07/01/ 2008. RESTA-LENERT, S.; BARRETT, K. E. Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive <em>Escherichia coli</em> (EIEC). 52, n. 7, p. 988-997, 2003. RINKINEN, M.; WESTERMARCK, E.; SALMINEN, S.; OUWEHAND, A. C. Absence of host specificity for in vitro adhesion of probiotic lactic acid bacteria to intestinal mucus. Veterinary Microbiology, 97, n. 1, p. 55-61, 2003/12/02/ 2003. ROUND, J. L.; MAZMANIAN, S. K. Inducible Foxp3+ regulatory T-cell development by a commensal bacterium of the intestinal microbiota. Proceedings of the National Academy of Sciences, 107, n. 27, p. 12204-12209, 2010. RUIZ-MOYANO, S.; GONÇALVES DOS SANTOS, M. T. P.; GALVÁN, A. I.; MERCHÁN, A. V. et al. Screening of autochthonous lactic acid bacteria strains from artisanal soft cheese: probiotic characteristics and prebiotic metabolism. LWT, 114, p. 108388, 2019/11/01/ 2019. SÁNCHEZ-TAPIA, M.; TOVAR, A. R.; TORRES, N. Diet as Regulator of Gut Microbiota and its Role in Health and Disease. Archives of Medical Research, 50, n. 5, p. 259-268, 2019/07/01/ 2019. SARIKAYA, H.; ASLIM, B.; YUKSEKDAG, Z. Assessment of anti-biofilm activity and bifidogenic growth stimulator (BGS) effect of lyophilized exopolysaccharides (l-EPSs) from Lactobacilli strains. International Journal of Food Properties, 20, n. 2, p. 362-371, 2017/02/01 2017. SERVIN, A. L. Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. 28, n. 4, p. 405-440, 2004. SHINDE, T.; VEMURI, R.; SHASTRI, M. D.; PERERA, A. P. et al. Probiotic Bacillus coagulans MTCC 5856 spores exhibit excellent in-vitro functional efficacy in simulated gastric survival, mucosal adhesion and immunomodulation. Journal of Functional Foods, 52, p. 100-108, 2019/01/01/ 2019. SLOVER, C. M.; DANZIGER, L. Lactobacillus: a Review. Clinical Microbiology Newsletter, 30, n. 4, p. 23-27, 2008/02/15/ 2008. TARRAH, A.; DA SILVA DUARTE, V.; DE CASTILHOS, J.; PAKROO, S. et al. Probiotic potential and biofilm inhibitory activity of Lactobacillus casei group strains isolated from infant feces. Journal of Functional Foods, 54, p. 489-497, 2019/03/01/ 2019. TASNIM, N.; ABULIZI, N.; PITHER, J.; HART, M. M. et al. Linking the Gut Microbial Ecosystem with the Environment: Does Gut Health Depend on Where We Live? Frontiers in Microbiology, 8, n. 1935, 2017-October-06 2017. Perspective. TODOROV, S. D.; BOTES, M.; GUIGAS, C.; SCHILLINGER, U. et al. Boza, a natural source of probiotic lactic acid bacteria. Journal of Applied Microbiology, 104, n. 2, p. 465-477, 2008/02/01 2008. TUO, Y.; SONG, X.; SONG, Y.; LIU, W. et al. Screening probiotics from Lactobacillus strains according to their abilities to inhibit pathogen adhesion and induction of pro-inflammatory cytokine IL-8. Journal of Dairy Science, 101, n. 6, p. 4822-4829, 2018/06/01/ 2018. VASCONCELOS, F. M.; SILVA, H. L. A.; POSO, S. M. V.; BARROSO, M. V. et al. Probiotic Prato cheese attenuates cigarette smoke-induced injuries in mice. Food Research International, 123, p. 697-703, 2019/09/01/ 2019. VASIEE, A.; MORTAZAVI, S. A.; SANKIAN, M.; YAZDI, F. T. et al. Antagonistic activity of recombinant Lactococcus lactis NZ1330 on the adhesion properties of Escherichia coli causing urinary tract infection. Microbial Pathogenesis, 133, p. 103547, 2019/08/01/ 2019. VIEIRA, M. A.; GUERRA, A. F.; LUCHESE, R. H. Mechanisms Of Pathogens Biofilm Reduction By Probiotic Bacteria. Revista Higiene Alimentar, Edição Especial, 2015. WANG, B.; LI, J.; CHEN, J.; HUANG, Q. et al. Effect of live Lactobacillus plantarum L2 on TNF-α-induced MCP-1 production in Caco-2 cells. International Journal of Food Microbiology, 142, n. 1, p. 237-241, 2010/08/15/ 2010. WANG, J.; ZHANG, H.; DU, H.; WANG, F. et al. Identification and characterization of Diutina rugosa SD-17 for potential use as a probiotic. LWT, 109, p. 283-288, 2019/07/01/ 2019. WOLTERS, M.; AHRENS, J.; ROMANÍ-PÉREZ, M.; WATKINS, C. et al. Dietary fat, the gut microbiota, and metabolic health – A systematic review conducted within the MyNewGut project. Clinical Nutrition, 38, n. 6, p. 2504-2520, 2019/12/01/ 2019. WU, M.-H.; PAN, T.-M.; WU, Y.-J.; CHANG, S.-J. et al. Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties. International Journal of Food Microbiology, 144, n. 1, p. 104-110, 2010/11/15/ 2010. XU, X.; BAO, Y.; WU, B.; LAO, F. et al. Chemical analysis and flavor properties of blended orange, carrot, apple and Chinese jujube juice fermented by selenium-enriched probiotics. Food Chemistry, 289, p. 250-258, 2019/08/15/ 2019. XU, Y.; ZHOU, T.; TANG, H.; LI, X. et al. Probiotic potential and amylolytic properties of lactic acid bacteria isolated from Chinese fermented cereal foods. Food Control, 111, p. 107057, 2020/05/01/ 2020. ZHANG, W.; WANG, H.; LIU, J.; ZHAO, Y. et al. Adhesive ability means inhibition activities for lactobacillus against pathogens and S-layer protein plays an important role in adhesion. Anaerobe, 22, p. 97-103, 2013/08/01/ 2013. ZHANG, Y.-C.; ZHANG, L.-W.; TUO, Y.-F.; GUO, C.-F. et al. Inhibition of Shigella sonnei adherence to HT-29 cells by lactobacilli from Chinese fermented food and preliminary characterization of S-layer protein involvement. Research in Microbiology, 161, n. 8, p. 667-672, 2010/10/01/ 2010. ZHANG, Z.; TAO, X.; SHAH, N. P.; WEI, H. Antagonistics against pathogenic Bacillus cereus in milk fermentation by Lactobacillus plantarum ZDY2013 and its anti-adhesion effect on Caco-2 cells against pathogens. Journal of Dairy Science, 99, n. 4, p. 2666-2674, 2016/04/01/ 2016. ZHENG, J.; WITTOUCK, S.; SALVETTI, E.; FRANZ, C. et al. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. International Journal of Systematic and Evolutionary Microbiology, 70, 04/15 2020.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
dc.publisher.initials.fl_str_mv UFRRJ
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Tecnologia
publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ
instname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
instname_str Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron_str UFRRJ
institution UFRRJ
reponame_str Biblioteca Digital de Teses e Dissertações da UFRRJ
collection Biblioteca Digital de Teses e Dissertações da UFRRJ
bitstream.url.fl_str_mv https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/1/2020%20-%20Matheus%20Rodrigues%20Silva%20do%20Carmo.pdf.jpg
https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/2/2020%20-%20Matheus%20Rodrigues%20Silva%20do%20Carmo.pdf.txt
https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/3/2020%20-%20Matheus%20Rodrigues%20Silva%20do%20Carmo.pdf
https://rima.ufrrj.br/jspui/bitstream/20.500.14407/11022/4/license.txt
bitstream.checksum.fl_str_mv cc73c4c239a4c332d642ba1e7c7a9fb2
a97f883ff38e9868d6672d016da57fe0
ad00c59b497b3faf56ab2fda9d9889e9
7b5ba3d2445355f386edab96125d42b7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)
repository.mail.fl_str_mv bibliot@ufrrj.br||bibliot@ufrrj.br
_version_ 1810108138627530752

Registros relacionados