Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2016v29n1p75 |
Resumo: | The contamination of aquatic environments by aluminum (Al) is usually caused by several anthropogenic activities. Diverse morphological, physiological and biochemical alterations in aquatic organisms have been attributed to exposure to Al. In this context, the aim of this study was to investigate the effects of acute exposure to different concentrations of Al on the histology of gills and frequency of micronucleus (MN) and nuclear abnormalities (NA) in erythrocytes of the fish Astyanax jacuhiensis. Fish were exposed to sublethal concentrations of 0.3 mg/L and 30 mg/L of aluminum at neutral pH for 72 h. A control group was kept in filtrated water. The alterations in the gills were characterized by epithelial hyperplasia and hypertrophy, lamellar fusion, edema, epithelial lifting, aneurism and necrosis. A significant increase (p<0.05) in the frequency of abnormal lamellae was observed in groups exposed to both concentrations of Al in comparison with the control group. On the other hand, no statistical differences were observed in the frequencies of MN and NA. Although no evidence of genotoxicity was observed, the results found in the gill morphological analysis suggest that Al was toxic to the fish at both concentrations tested under neutral pH. |
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Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminumHistopatologia de brânquias e teste de micronúcleos em Astyanax jacuhiensis(Cope, 1894) (Teleostei, Characidae) na avaliação dos efeitos causados pela exposição aguda ao alumínioThe contamination of aquatic environments by aluminum (Al) is usually caused by several anthropogenic activities. Diverse morphological, physiological and biochemical alterations in aquatic organisms have been attributed to exposure to Al. In this context, the aim of this study was to investigate the effects of acute exposure to different concentrations of Al on the histology of gills and frequency of micronucleus (MN) and nuclear abnormalities (NA) in erythrocytes of the fish Astyanax jacuhiensis. Fish were exposed to sublethal concentrations of 0.3 mg/L and 30 mg/L of aluminum at neutral pH for 72 h. A control group was kept in filtrated water. The alterations in the gills were characterized by epithelial hyperplasia and hypertrophy, lamellar fusion, edema, epithelial lifting, aneurism and necrosis. A significant increase (p<0.05) in the frequency of abnormal lamellae was observed in groups exposed to both concentrations of Al in comparison with the control group. On the other hand, no statistical differences were observed in the frequencies of MN and NA. Although no evidence of genotoxicity was observed, the results found in the gill morphological analysis suggest that Al was toxic to the fish at both concentrations tested under neutral pH.A contaminação de ecossistemas aquáticos por alumínio (Al) é geralmente ocasionada por várias atividades antropogênicas. Diversas alterações morfológicas, fisiológicas e bioquímicas observadas em organismos aquáticos podem ser atribuídas à exposição ao Al. Neste contexto, o objetivo desse estudo foi investigar os efeitos da exposição aguda de peixes da espécie Astyanax jacuhiensis a diferentes concentrações de Al através da histologia de brânquias e frequência de micronúcleos (MN) e anormalidades nucleares (AN) em eritrócitos. Os animais foram expostos às concentrações subletais de 0.3 mg/L e 30 mg/L de alumínio em pH neutro por 72 h. Um grupo controle foi mantido em água filtrada. As alterações branquiais foram caracterizadas por hiperplasia e hipertrofia de células epiteliais, fusão lamelar, edema, descolamento epitelial, aneurisma e necrose. Um aumento significativo (p<0.05) foi observado na frequência de lamelas alteradas nos grupos expostos às duas concentrações de Al em comparação aos animais controle. No entanto, não foram observadas diferenças significativas nas frequências de MN e AN. Embora não tenham sido encontradas evidências de genotoxicidade, os resultados encontrados na análise morfológica sugerem que o Al foi tóxico para os peixes nas duas concentrações testadas em pH neutro. Universidade Federal de Santa Catarina2016-02-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2016v29n1p7510.5007/2175-7925.2016v29n1p75Biotemas; v. 29 n. 1 (2016); 75-832175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCenghttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2016v29n1p75/31233Copyright (c) 2016 Thaís Dalzochio, Angélica Goldoni, Gabriela Zimmermann Prado Rodrigues, Ismael Evandro Petry, Luciano Basso da Silva, Günther Gehleninfo:eu-repo/semantics/openAccessDalzochio, ThaísGoldoni, AngélicaZimmermann Prado Rodrigues, GabrielaPetry, Ismael EvandroBasso da Silva, LucianoGehlen, Günther2017-10-18T19:02:14Zoai:periodicos.ufsc.br:article/38065Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-18T19:02:14Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum Histopatologia de brânquias e teste de micronúcleos em Astyanax jacuhiensis(Cope, 1894) (Teleostei, Characidae) na avaliação dos efeitos causados pela exposição aguda ao alumínio |
title |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum |
spellingShingle |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum Dalzochio, Thaís |
title_short |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum |
title_full |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum |
title_fullStr |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum |
title_full_unstemmed |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum |
title_sort |
Gill histopathology and micronucleus test of Astyanax jacuhiensis (Cope, 1894) (Teleostei, Characidae) to evaluate effects caused by acute exposure to aluminum |
author |
Dalzochio, Thaís |
author_facet |
Dalzochio, Thaís Goldoni, Angélica Zimmermann Prado Rodrigues, Gabriela Petry, Ismael Evandro Basso da Silva, Luciano Gehlen, Günther |
author_role |
author |
author2 |
Goldoni, Angélica Zimmermann Prado Rodrigues, Gabriela Petry, Ismael Evandro Basso da Silva, Luciano Gehlen, Günther |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dalzochio, Thaís Goldoni, Angélica Zimmermann Prado Rodrigues, Gabriela Petry, Ismael Evandro Basso da Silva, Luciano Gehlen, Günther |
description |
The contamination of aquatic environments by aluminum (Al) is usually caused by several anthropogenic activities. Diverse morphological, physiological and biochemical alterations in aquatic organisms have been attributed to exposure to Al. In this context, the aim of this study was to investigate the effects of acute exposure to different concentrations of Al on the histology of gills and frequency of micronucleus (MN) and nuclear abnormalities (NA) in erythrocytes of the fish Astyanax jacuhiensis. Fish were exposed to sublethal concentrations of 0.3 mg/L and 30 mg/L of aluminum at neutral pH for 72 h. A control group was kept in filtrated water. The alterations in the gills were characterized by epithelial hyperplasia and hypertrophy, lamellar fusion, edema, epithelial lifting, aneurism and necrosis. A significant increase (p<0.05) in the frequency of abnormal lamellae was observed in groups exposed to both concentrations of Al in comparison with the control group. On the other hand, no statistical differences were observed in the frequencies of MN and NA. Although no evidence of genotoxicity was observed, the results found in the gill morphological analysis suggest that Al was toxic to the fish at both concentrations tested under neutral pH. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2016v29n1p75 10.5007/2175-7925.2016v29n1p75 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2016v29n1p75 |
identifier_str_mv |
10.5007/2175-7925.2016v29n1p75 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2016v29n1p75/31233 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 29 n. 1 (2016); 75-83 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940553592274944 |