Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/75404 |
Resumo: | The proposal of this study is a protocol for the preparation of permanent sheets of protists and rotifers by adapting a Kisser glyceryn-jelly technique in three stages. A total of 450 sheets of protists and rotifers were made. After a period of 2 years, the slides remained stable, with no signs of fungal contamination and the organisms maintained their integral morphological conformation. The proposed technique is promising for the conservation of the morphological characteristics of the specimens, presenting low risk of manipulation, low cost, few reagents and easy handling, allowing the creation of a didactic collection for practical classes. |
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Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposesGelatina glicerinada como meio de montagem de lâminas permanentes de protistas e rotíferos: uma proposta para fins didáticosThe proposal of this study is a protocol for the preparation of permanent sheets of protists and rotifers by adapting a Kisser glyceryn-jelly technique in three stages. A total of 450 sheets of protists and rotifers were made. After a period of 2 years, the slides remained stable, with no signs of fungal contamination and the organisms maintained their integral morphological conformation. The proposed technique is promising for the conservation of the morphological characteristics of the specimens, presenting low risk of manipulation, low cost, few reagents and easy handling, allowing the creation of a didactic collection for practical classes.Propor protocolo de preparo de lâminas permanentes de protistas e rotíferos através da adaptação da técnica de gelatina glicerinada de Kisser, composta por três etapas. Um total de 450 lâminas de protistas e rotíferos foram confeccionadas. Após um período de 2 anos, as lâminas permaneceram estáveis, sem sinais de contaminação por fungos e os organismos mantiveram sua conformação morfológica íntegra. Técnica proposta é promissora quanto a conservação das características morfológicas dos espécimes, apresentando baixo risco de manipulação, baixo custo, poucos reagentes e fácil manuseio, permitindo a construção de um acervo didático para aulas práticas.Universidade Federal de Santa Catarina2020-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/7540410.5007/2175-7925.2020.e75404Biotemas; v. 33 n. 4 (2020); 1-62175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/75404/44837Copyright (c) 2020 Márcio Borba da Silva, Caique Santos Aguiar, Cínthya Souza Rocha, Sara Barbosa Santos, Matheus Santos dos Anjos, Rafael Sampaio Vieira Silva, Patrícia Belini Nishiyama, Ricardo Evangelista Fraga, Mariane Amorim Rocha, Paulino Pereira Oliveirahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Márcio Borba daAguiar, Caique Dos SantosRocha, Cínthya Viana SouzaSantos, Sara BarbosaAnjos, Matheus Santos dosSilva, Rafael Sampaio VieiraNishiyama, Patrícia BeliniFraga, Ricardo EvangelistaRocha, Mariane AmorimOliveira, Paulino Pereira2020-12-14T18:48:23Zoai:periodicos.ufsc.br:article/75404Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2020-12-14T18:48:23Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes Gelatina glicerinada como meio de montagem de lâminas permanentes de protistas e rotíferos: uma proposta para fins didáticos |
title |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes |
spellingShingle |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes Silva, Márcio Borba da |
title_short |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes |
title_full |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes |
title_fullStr |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes |
title_full_unstemmed |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes |
title_sort |
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes |
author |
Silva, Márcio Borba da |
author_facet |
Silva, Márcio Borba da Aguiar, Caique Dos Santos Rocha, Cínthya Viana Souza Santos, Sara Barbosa Anjos, Matheus Santos dos Silva, Rafael Sampaio Vieira Nishiyama, Patrícia Belini Fraga, Ricardo Evangelista Rocha, Mariane Amorim Oliveira, Paulino Pereira |
author_role |
author |
author2 |
Aguiar, Caique Dos Santos Rocha, Cínthya Viana Souza Santos, Sara Barbosa Anjos, Matheus Santos dos Silva, Rafael Sampaio Vieira Nishiyama, Patrícia Belini Fraga, Ricardo Evangelista Rocha, Mariane Amorim Oliveira, Paulino Pereira |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Márcio Borba da Aguiar, Caique Dos Santos Rocha, Cínthya Viana Souza Santos, Sara Barbosa Anjos, Matheus Santos dos Silva, Rafael Sampaio Vieira Nishiyama, Patrícia Belini Fraga, Ricardo Evangelista Rocha, Mariane Amorim Oliveira, Paulino Pereira |
description |
The proposal of this study is a protocol for the preparation of permanent sheets of protists and rotifers by adapting a Kisser glyceryn-jelly technique in three stages. A total of 450 sheets of protists and rotifers were made. After a period of 2 years, the slides remained stable, with no signs of fungal contamination and the organisms maintained their integral morphological conformation. The proposed technique is promising for the conservation of the morphological characteristics of the specimens, presenting low risk of manipulation, low cost, few reagents and easy handling, allowing the creation of a didactic collection for practical classes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/75404 10.5007/2175-7925.2020.e75404 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/75404 |
identifier_str_mv |
10.5007/2175-7925.2020.e75404 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/75404/44837 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 33 n. 4 (2020); 1-6 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940554553819136 |