Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Saúde (Santa Maria) |
Texto Completo: | https://periodicos.ufsm.br/revistasaude/article/view/67777 |
Resumo: | Objective: This study aims to evaluate the antimicrobial potential of essential oils from Eucalyptus globulus and Citrus sinensis against Aeromonas hydrophila, one of the microorganisms that is related to infections in fish. Methods: Disk diffusion tests were performed with 10 µl of essential oils against the inoculum of A. hydrophila on the McFarland 0.5 scale, in addition to the Minimum Inhibitory Concentration (MIC) through the microdilution technique in 96-well plates and the Bactericide Minimum Concentration (MBC) by removing an aliquot of the MIC from each oil and inoculating into a plate containing the Mueller-Hinton medium. Results: Mean inhibition halos in triplicate were 17 mm with E. globulus essential oil and 25.7 mm with C. sinensis essential oil, in addition to a MIC of 5.758 µg / ml (w/v) with the essential oil of E. globulus and 330.7 µg / ml with the essential oil of C. sinensis, in addition to CBM of 5.758 µg / ml (w/v) with the essential oil of E. globulus and 2645 µg / ml (w/v) with the essential oil of C. sinensis against A. hydrophila. Conclusion: E. globulus and C. sinensis essential oils have antimicrobial potential against A. hydrophila bacteria, and can be used in fish farming, reducing bacterial contamination of animals and subsequent human contamination through the consumption of their meat. |
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Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophilaAvaliação da atividade antimicrobiana dos óleos essenciais do Eucalyptus globulus e Citrus sinensis frente a Aeromonas hydrophilaEucalyptusCitrus sinensisAeromonas hydrophilaÓleos voláteisProdutos com ação antimicrobianaEucalyptusCitrus sinensisAeromonas hydrophilaOils, volatileProducts with antimicrobial actionObjective: This study aims to evaluate the antimicrobial potential of essential oils from Eucalyptus globulus and Citrus sinensis against Aeromonas hydrophila, one of the microorganisms that is related to infections in fish. Methods: Disk diffusion tests were performed with 10 µl of essential oils against the inoculum of A. hydrophila on the McFarland 0.5 scale, in addition to the Minimum Inhibitory Concentration (MIC) through the microdilution technique in 96-well plates and the Bactericide Minimum Concentration (MBC) by removing an aliquot of the MIC from each oil and inoculating into a plate containing the Mueller-Hinton medium. Results: Mean inhibition halos in triplicate were 17 mm with E. globulus essential oil and 25.7 mm with C. sinensis essential oil, in addition to a MIC of 5.758 µg / ml (w/v) with the essential oil of E. globulus and 330.7 µg / ml with the essential oil of C. sinensis, in addition to CBM of 5.758 µg / ml (w/v) with the essential oil of E. globulus and 2645 µg / ml (w/v) with the essential oil of C. sinensis against A. hydrophila. Conclusion: E. globulus and C. sinensis essential oils have antimicrobial potential against A. hydrophila bacteria, and can be used in fish farming, reducing bacterial contamination of animals and subsequent human contamination through the consumption of their meat.Objetivo: Esse estudo tem o objetivo de avaliar o potencial antimicrobiano dos óleos essenciais de Eucalyptus globulus e Citrus sinensis frente a Aeromonas hydrophila, um dos microrganismos que está relacionado às infecções em peixes. Métodos: Realizou-se os testes de disco difusão com 10 μl dos óleos essenciais frente ao inóculo de A. hydrophila na escala 0,5 de McFarland, além da Concentração Inibitória Mínima (CIM) através da técnica de microdiluição em placas de 96 poços e da Concentração Bactericida Mínima (CBM) retirando-se uma alíquota da CIM de cada óleo e inoculando em placa contendo o meio Mueller-Hinton. Resultados: A média dos halos de inibição em triplicata foram de 17 mm com o óleo essencial de E. globulus e de 25,7 mm com o óleo essencial de C. sinensis, ademais se obteve uma CIM de 5.758 μg/ml (m/v) com o óleo essencial de E. globulus e 330,7 μg/ml com o óleo essencial de C. sinensis, além de uma CBM de 5.758 μg/ml (m/v) com o óleo essencial de E. globulus e de 2.645 μg/ml (m/v) com o óleo essencial de C. sinensis frente a A. hydrophila. Considerações finais: Os óleos essenciais de E. globulus e C. sinensis possuem potencial antimicrobiano frente a bactéria A. hydrophila, podendo ser administrados no cultivo de peixes, diminuindo a contaminação bacteriana dos animais e posterior contaminação humana através do consumo de sua carne.Universidade Federal de Santa Maria2024-04-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/revistasaude/article/view/6777710.5902/2236583467777Saúde (Santa Maria); Vol. 50 No. 1 (2024): Revista Saúde (Santa Maria) | Fluxo contínuo; e67777Saúde (Santa Maria); v. 50 n. 1 (2024): Revista Saúde (Santa Maria) | Fluxo contínuo; e677772236-58340103-4499reponame:Saúde (Santa Maria)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/revistasaude/article/view/67777/63712Copyright (c) 2024 Saúde (Santa Maria)https://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessPeixoto, TaynaTeixeira, Cristiane AntunesVerdi, Camila MarinaBarin, Thais de MoraesSchneider, Taiane2024-04-24T19:33:13Zoai:ojs.pkp.sfu.ca:article/67777Revistahttps://periodicos.ufsm.br/revistasaudePUBhttps://periodicos.ufsm.br/revistasaude/oairevistasaude.ufsm@gmail.com || amanda.revsaude@gmail.com || beatriz.revsaude@gmail.com2236-58342236-5834opendoar:2024-04-24T19:33:13Saúde (Santa Maria) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila Avaliação da atividade antimicrobiana dos óleos essenciais do Eucalyptus globulus e Citrus sinensis frente a Aeromonas hydrophila |
title |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila |
spellingShingle |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila Peixoto, Tayna Eucalyptus Citrus sinensis Aeromonas hydrophila Óleos voláteis Produtos com ação antimicrobiana Eucalyptus Citrus sinensis Aeromonas hydrophila Oils, volatile Products with antimicrobial action |
title_short |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila |
title_full |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila |
title_fullStr |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila |
title_full_unstemmed |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila |
title_sort |
Evaluation of the antimicrobial activity of the essential oils of Eucalyptus globulus and Citrus sinensis in front of Aeromonas hydrophila |
author |
Peixoto, Tayna |
author_facet |
Peixoto, Tayna Teixeira, Cristiane Antunes Verdi, Camila Marina Barin, Thais de Moraes Schneider, Taiane |
author_role |
author |
author2 |
Teixeira, Cristiane Antunes Verdi, Camila Marina Barin, Thais de Moraes Schneider, Taiane |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Peixoto, Tayna Teixeira, Cristiane Antunes Verdi, Camila Marina Barin, Thais de Moraes Schneider, Taiane |
dc.subject.por.fl_str_mv |
Eucalyptus Citrus sinensis Aeromonas hydrophila Óleos voláteis Produtos com ação antimicrobiana Eucalyptus Citrus sinensis Aeromonas hydrophila Oils, volatile Products with antimicrobial action |
topic |
Eucalyptus Citrus sinensis Aeromonas hydrophila Óleos voláteis Produtos com ação antimicrobiana Eucalyptus Citrus sinensis Aeromonas hydrophila Oils, volatile Products with antimicrobial action |
description |
Objective: This study aims to evaluate the antimicrobial potential of essential oils from Eucalyptus globulus and Citrus sinensis against Aeromonas hydrophila, one of the microorganisms that is related to infections in fish. Methods: Disk diffusion tests were performed with 10 µl of essential oils against the inoculum of A. hydrophila on the McFarland 0.5 scale, in addition to the Minimum Inhibitory Concentration (MIC) through the microdilution technique in 96-well plates and the Bactericide Minimum Concentration (MBC) by removing an aliquot of the MIC from each oil and inoculating into a plate containing the Mueller-Hinton medium. Results: Mean inhibition halos in triplicate were 17 mm with E. globulus essential oil and 25.7 mm with C. sinensis essential oil, in addition to a MIC of 5.758 µg / ml (w/v) with the essential oil of E. globulus and 330.7 µg / ml with the essential oil of C. sinensis, in addition to CBM of 5.758 µg / ml (w/v) with the essential oil of E. globulus and 2645 µg / ml (w/v) with the essential oil of C. sinensis against A. hydrophila. Conclusion: E. globulus and C. sinensis essential oils have antimicrobial potential against A. hydrophila bacteria, and can be used in fish farming, reducing bacterial contamination of animals and subsequent human contamination through the consumption of their meat. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-04-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/revistasaude/article/view/67777 10.5902/2236583467777 |
url |
https://periodicos.ufsm.br/revistasaude/article/view/67777 |
identifier_str_mv |
10.5902/2236583467777 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/revistasaude/article/view/67777/63712 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2024 Saúde (Santa Maria) https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2024 Saúde (Santa Maria) https://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Saúde (Santa Maria); Vol. 50 No. 1 (2024): Revista Saúde (Santa Maria) | Fluxo contínuo; e67777 Saúde (Santa Maria); v. 50 n. 1 (2024): Revista Saúde (Santa Maria) | Fluxo contínuo; e67777 2236-5834 0103-4499 reponame:Saúde (Santa Maria) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Saúde (Santa Maria) |
collection |
Saúde (Santa Maria) |
repository.name.fl_str_mv |
Saúde (Santa Maria) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
revistasaude.ufsm@gmail.com || amanda.revsaude@gmail.com || beatriz.revsaude@gmail.com |
_version_ |
1799943998350032896 |