Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11122 |
Resumo: | Rheumatoid arthritis (RA) is a chronic inflammatory disease autoimmune of unknown etiology. Patients with RA have a lower survival than the general population, the most common cause of mortality in these patients is cardiovascular disease. The systemic inflammation that occurs in RA produces a spectrum of proatherogenic changes including endothelial dysfunction, insulin resistance, dyslipidemia, prothrombotic effects and pro-oxidative stress. Endothelial dysfunction is the key event in early atherogenesis. The platelets are known to have an important role in the maintenance of endothelial integrity and homeostasis, and also are key players in atherothrombosis. Extracellular adenine nucleotides ATP, ADP and AMP and the nucleoside adenosine, modulate a variety of tissue effects, exerting several effects on the platelets. This work determined the activity of enzymes involved in the degradation of nucleotides and nucleosides (NTPDase, 5 -nucleotidase E-NPP and ADA) in platelets from patients with RA and controls patients. It was selected thirty-five RA patients from the Hospital of the Federal University of Santa Maria and the diagnostic was based on the criteria of American College of Rheumatology. The results showed an increase in the activity of NTPDase (approximately 100%), 5 -nucleotidase (170%), E-NPP (approximately 100%) and ADA (approximately 45%) in the platelets of patients with RA when compared to control group. The increased activity of these enzymes could be related to a compensatory response of the organism due to the disease. The platelet aggregation was also determined in these patients and no changes were found when compared to the control group. The increase in ADA activity was lower when compared to the increase observed in the other enzymes activities (NTPDase, 5 -nucleotidase and ENPP). Possibly not all generated adenosine is converted by the ADA, which could explain the normality of platelet aggregation in patients with RA. No interference of the drugs (methotrexate, leflunomide,hydroxychloroquine and prednisone) utilized in RA treatment was observed on the enzymatic activities tested in vitro. In this way, it is possible to conclude that the NTPDase, 5 -nucleotidase, E-NPP and ADA activities are altered in platelets of patients with RA, which could be considered a compensatory response against the disease. |
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2017-04-242017-04-242010-03-26BECKER, Lara Vargas. Activities of enzymes that hydrolyze adenine nucleotides in plateles from patients with rheumatoid arthritis. 2010. 90 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/11122Rheumatoid arthritis (RA) is a chronic inflammatory disease autoimmune of unknown etiology. Patients with RA have a lower survival than the general population, the most common cause of mortality in these patients is cardiovascular disease. The systemic inflammation that occurs in RA produces a spectrum of proatherogenic changes including endothelial dysfunction, insulin resistance, dyslipidemia, prothrombotic effects and pro-oxidative stress. Endothelial dysfunction is the key event in early atherogenesis. The platelets are known to have an important role in the maintenance of endothelial integrity and homeostasis, and also are key players in atherothrombosis. Extracellular adenine nucleotides ATP, ADP and AMP and the nucleoside adenosine, modulate a variety of tissue effects, exerting several effects on the platelets. This work determined the activity of enzymes involved in the degradation of nucleotides and nucleosides (NTPDase, 5 -nucleotidase E-NPP and ADA) in platelets from patients with RA and controls patients. It was selected thirty-five RA patients from the Hospital of the Federal University of Santa Maria and the diagnostic was based on the criteria of American College of Rheumatology. The results showed an increase in the activity of NTPDase (approximately 100%), 5 -nucleotidase (170%), E-NPP (approximately 100%) and ADA (approximately 45%) in the platelets of patients with RA when compared to control group. The increased activity of these enzymes could be related to a compensatory response of the organism due to the disease. The platelet aggregation was also determined in these patients and no changes were found when compared to the control group. The increase in ADA activity was lower when compared to the increase observed in the other enzymes activities (NTPDase, 5 -nucleotidase and ENPP). Possibly not all generated adenosine is converted by the ADA, which could explain the normality of platelet aggregation in patients with RA. No interference of the drugs (methotrexate, leflunomide,hydroxychloroquine and prednisone) utilized in RA treatment was observed on the enzymatic activities tested in vitro. In this way, it is possible to conclude that the NTPDase, 5 -nucleotidase, E-NPP and ADA activities are altered in platelets of patients with RA, which could be considered a compensatory response against the disease.A artrite reumatóide (AR) é uma doença inflamatória crônica autoimune, de etiologia desconhecida. Os pacientes portadores de AR têm uma sobrevida menor do que a da população em geral, sendo que a causa mais comum de mortalidade é a doença cardiovascular. A inflamação sistêmica que ocorre na AR produz um espectro de mudanças proaterogênicas que incluem a disfunção endotelial, a resistência à insulina, a dislipidemia, os efeitos protrombóticos e o estresse oxidativo. A disfunção endotelial é um evento chave no início da aterogênese. As plaquetas são conhecidas por ter um importante papel na manutenção da integridade endotelial e da hemostasia, possuindo um papel chave na aterotrombose. Os nucleotídeos extracelulares de adenina ATP, ADP e AMP e o nucleosídeo adenosina, modulam uma variedade de efeitos teciduais, além de exercererm diversos efeitos nas plaquetas. Neste trabalho foi determinada a atividade das enzimas envolvidas na degradação de nucleotídeos e nucleosídeos (NTPDase, 5 -nucleotidase, E-NPP e ADA) em plaquetas de pacientes com AR. Foram selecionados 35 pacientes do Hospital Universitário de Santa Maria com diagnóstico de AR, o qual foi baseado nos critérios de classificação do Colégio Americano de Reumatologia. Os resultados demonstraram um aumento na atividade das enzimas NTPDase (aproximadamente 100%), 5 -nucleotidase (170%), E-NPP (aproximadamente 100%) e ADA (aproximadamente 45%) em plaquetas de pacientes com AR quando comparado ao controle. O aumento da atividade dessas enzimas pode estar relacionado com uma resposta compensatória do organismo frente à doença. Também foi determinado o perfil da agregação plaquetária nesses pacientes, o qual encontrou-se inalterado quando comparado ao controle. O aumento da atividade da enzima ADA foi menor quando comparado ao aumento das atividades das outras enzimas (NTPDase, 5 -nucleotidase e E-NPP). Possivelmente, nem toda a adenosina gerada é convertida pela ADA, o que poderia explicar a normalidade da agregação plaquetária nos pacientes estudados. Não foi observada nenhuma interferência dos fármacos (hidroxicloroquina, leflunomida, metotrexato e prednisona) utilizados no tratamento de AR sobre a atividade das enzimas testadas in vitro. Concluiu-se que a atividade das enzimas NTPDase, 5 -nucleotidase, E-NPP e ADA encontram-se alteradas em plaquetas de pacientes com AR, o que poderia ser considerado uma resposta compensatória do organismo frente à doença.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaArtrite reumatóideNTPDase5'-nucleotidaseE-NPPADAPlaquetasAteroscleroseRheumatoid arthritisPlateletsAtherosclerosisCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAtividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóideActivities of enzymes that hydrolyze adenine nucleotides in plateles from patients with rheumatoid arthritisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSchetinger, Maria Rosa ChitolinaLeal, Daniela Bitencourt Rosahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7Bonan, Carla Denisehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799528J3Loro, Vania Luciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796333D7http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4710016E5Becker, Lara Vargas200800000002400500300300500500f2c5f39c-089e-4825-bfba-cc66b9098981bcdffe28-e6ed-41e1-bf64-bf0bbe95898b273fd05e-83b4-4374-81a7-26d005e91f4b64d05617-eaff-4cb5-8519-d7dc8a467932301a3550-6b95-45aa-be7f-19ffad13abc4info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALBECKER, LARA VARGAS.pdfapplication/pdf694412http://repositorio.ufsm.br/bitstream/1/11122/1/BECKER%2c%20LARA%20VARGAS.pdf08958b6c2d2645ca1efc3c0470081f04MD51TEXTBECKER, LARA VARGAS.pdf.txtBECKER, LARA VARGAS.pdf.txtExtracted texttext/plain133370http://repositorio.ufsm.br/bitstream/1/11122/2/BECKER%2c%20LARA%20VARGAS.pdf.txtaadfd7eb86e1cbf31e805fac57e2449aMD52THUMBNAILBECKER, LARA VARGAS.pdf.jpgBECKER, LARA VARGAS.pdf.jpgIM Thumbnailimage/jpeg4812http://repositorio.ufsm.br/bitstream/1/11122/3/BECKER%2c%20LARA%20VARGAS.pdf.jpgf241e1e1eee3172e82bf6e1c649f445eMD531/111222017-07-26 23:21:16.035oai:repositorio.ufsm.br:1/11122Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132017-07-27T02:21:16Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide |
dc.title.alternative.eng.fl_str_mv |
Activities of enzymes that hydrolyze adenine nucleotides in plateles from patients with rheumatoid arthritis |
title |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide |
spellingShingle |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide Becker, Lara Vargas Artrite reumatóide NTPDase 5'-nucleotidase E-NPP ADA Plaquetas Aterosclerose Rheumatoid arthritis Platelets Atherosclerosis CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide |
title_full |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide |
title_fullStr |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide |
title_full_unstemmed |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide |
title_sort |
Atividade de enzimas que hidrolisam nucleotídeos de adenina em plaquetas de pacientes com artrite reumatóide |
author |
Becker, Lara Vargas |
author_facet |
Becker, Lara Vargas |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.advisor-co1.fl_str_mv |
Leal, Daniela Bitencourt Rosa |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7 |
dc.contributor.referee1.fl_str_mv |
Bonan, Carla Denise |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799528J3 |
dc.contributor.referee2.fl_str_mv |
Loro, Vania Lucia |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796333D7 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4710016E5 |
dc.contributor.author.fl_str_mv |
Becker, Lara Vargas |
contributor_str_mv |
Schetinger, Maria Rosa Chitolina Leal, Daniela Bitencourt Rosa Bonan, Carla Denise Loro, Vania Lucia |
dc.subject.por.fl_str_mv |
Artrite reumatóide NTPDase 5'-nucleotidase E-NPP ADA Plaquetas Aterosclerose |
topic |
Artrite reumatóide NTPDase 5'-nucleotidase E-NPP ADA Plaquetas Aterosclerose Rheumatoid arthritis Platelets Atherosclerosis CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Rheumatoid arthritis Platelets Atherosclerosis |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Rheumatoid arthritis (RA) is a chronic inflammatory disease autoimmune of unknown etiology. Patients with RA have a lower survival than the general population, the most common cause of mortality in these patients is cardiovascular disease. The systemic inflammation that occurs in RA produces a spectrum of proatherogenic changes including endothelial dysfunction, insulin resistance, dyslipidemia, prothrombotic effects and pro-oxidative stress. Endothelial dysfunction is the key event in early atherogenesis. The platelets are known to have an important role in the maintenance of endothelial integrity and homeostasis, and also are key players in atherothrombosis. Extracellular adenine nucleotides ATP, ADP and AMP and the nucleoside adenosine, modulate a variety of tissue effects, exerting several effects on the platelets. This work determined the activity of enzymes involved in the degradation of nucleotides and nucleosides (NTPDase, 5 -nucleotidase E-NPP and ADA) in platelets from patients with RA and controls patients. It was selected thirty-five RA patients from the Hospital of the Federal University of Santa Maria and the diagnostic was based on the criteria of American College of Rheumatology. The results showed an increase in the activity of NTPDase (approximately 100%), 5 -nucleotidase (170%), E-NPP (approximately 100%) and ADA (approximately 45%) in the platelets of patients with RA when compared to control group. The increased activity of these enzymes could be related to a compensatory response of the organism due to the disease. The platelet aggregation was also determined in these patients and no changes were found when compared to the control group. The increase in ADA activity was lower when compared to the increase observed in the other enzymes activities (NTPDase, 5 -nucleotidase and ENPP). Possibly not all generated adenosine is converted by the ADA, which could explain the normality of platelet aggregation in patients with RA. No interference of the drugs (methotrexate, leflunomide,hydroxychloroquine and prednisone) utilized in RA treatment was observed on the enzymatic activities tested in vitro. In this way, it is possible to conclude that the NTPDase, 5 -nucleotidase, E-NPP and ADA activities are altered in platelets of patients with RA, which could be considered a compensatory response against the disease. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-03-26 |
dc.date.accessioned.fl_str_mv |
2017-04-24 |
dc.date.available.fl_str_mv |
2017-04-24 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BECKER, Lara Vargas. Activities of enzymes that hydrolyze adenine nucleotides in plateles from patients with rheumatoid arthritis. 2010. 90 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11122 |
identifier_str_mv |
BECKER, Lara Vargas. Activities of enzymes that hydrolyze adenine nucleotides in plateles from patients with rheumatoid arthritis. 2010. 90 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
url |
http://repositorio.ufsm.br/handle/1/11122 |
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Universidade Federal de Santa Maria |
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