Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/4859 |
Resumo: | In Brazil, the economic potential of the germplasm of medicinal species is a treasure to be preserved and utilized, increasingly becoming a form of alternative therapy available for the population to use. However, the indiscriminate and uncontrolled use can, cause more harm than benefits to health and it is important to understand these plants, from cellular levels and the action on living organisms. The medicinal species Echinodorus grandiflorus and Sagittaria montevidensis belong to the family Alismataceae, known respectively as leather hat and false leather hat in Brazil. For E. grandiflorus the attributed medicinal potentials are depurative, diuretic, anti-inflammatory, antiophidic and anti-rheumatic action, were leaves are the main part of the plant used, either as a tea or infusion. S. montevidensis has no known medicinal properties and having a wide range of leaf morphologies, leading to population confused with leather hat . The present study evaluated the genotoxic and antiproliferative extracts of the dried leaves of E. grandiflorus and S. montevidensis using the cell cycle of Allium cepa, and performing analysis by high performance liquid chromatography (HPLC-DAD) for the quantification of the extracts of the compounds. The test system is well suited for the analysis of genotoxicity, being validated by the International Programme on Chemical Safety (IPCS, WHO) and can alert on the possible damages that can be caused to organisms exposed to the substances in the plants. For the test, plant material was collected in the field in Rio Grande do Sul, in the municipalities of: Santa Maria, São Sepé, Silveira Martins and Pinhal Grande and in the commercial form of E. grandiflorus, separated into two experimental groups. The extracts were prepared, by infusing dry leaves for 15 min., at two concentrations, 6g.L-1 e 24g.L-1, and two controls, water (negative control) and glyphosate 15% (positive control). The chromatographic analysis (HPLC-DAD), revealed the presence of flavonoids and phenolic acids for both species, higher concentrations of caffeic acid and flavonoid glycoside in E. grandiflorus and phenol glycoside in S. montevidensis. This study showed that extracts of E. grandiflorus and S. montevidensis show genotoxic potential and when used in high concentrations, antiproliferative potential. |
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2014-02-132014-02-132013-02-18COELHO, Ana Paula Durand. Potential genotoxicity and antiproliferative of extracts Echinodorus grandiflorus and Sagittaria montevidensis (Alismataceae). 2013. 58 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/4859In Brazil, the economic potential of the germplasm of medicinal species is a treasure to be preserved and utilized, increasingly becoming a form of alternative therapy available for the population to use. However, the indiscriminate and uncontrolled use can, cause more harm than benefits to health and it is important to understand these plants, from cellular levels and the action on living organisms. The medicinal species Echinodorus grandiflorus and Sagittaria montevidensis belong to the family Alismataceae, known respectively as leather hat and false leather hat in Brazil. For E. grandiflorus the attributed medicinal potentials are depurative, diuretic, anti-inflammatory, antiophidic and anti-rheumatic action, were leaves are the main part of the plant used, either as a tea or infusion. S. montevidensis has no known medicinal properties and having a wide range of leaf morphologies, leading to population confused with leather hat . The present study evaluated the genotoxic and antiproliferative extracts of the dried leaves of E. grandiflorus and S. montevidensis using the cell cycle of Allium cepa, and performing analysis by high performance liquid chromatography (HPLC-DAD) for the quantification of the extracts of the compounds. The test system is well suited for the analysis of genotoxicity, being validated by the International Programme on Chemical Safety (IPCS, WHO) and can alert on the possible damages that can be caused to organisms exposed to the substances in the plants. For the test, plant material was collected in the field in Rio Grande do Sul, in the municipalities of: Santa Maria, São Sepé, Silveira Martins and Pinhal Grande and in the commercial form of E. grandiflorus, separated into two experimental groups. The extracts were prepared, by infusing dry leaves for 15 min., at two concentrations, 6g.L-1 e 24g.L-1, and two controls, water (negative control) and glyphosate 15% (positive control). The chromatographic analysis (HPLC-DAD), revealed the presence of flavonoids and phenolic acids for both species, higher concentrations of caffeic acid and flavonoid glycoside in E. grandiflorus and phenol glycoside in S. montevidensis. This study showed that extracts of E. grandiflorus and S. montevidensis show genotoxic potential and when used in high concentrations, antiproliferative potential.No Brasil, o potencial econômico de germoplasma de espécies medicinais é uma riqueza a ser preservada e utilizada, tornando-se cada vez mais uma forma de terapia alternativa acessível para o uso da população. No entanto, o uso indiscriminado e sem controle pode causar mais danos à saúde do que benefícios, sendo importante o conhecimento dessas plantas, desde os níveis celulares bem como ação sobre os organismos vivos. As espécies medicinais Echinodorus grandiflorus e Sagittaria montevidensis pertencem à família Alismataceae, conhecidas respectivamente como chapéu-de-couro e falso chapéu-de-couro. Para E. grandiflorus é atribuído potencial medicinal como ações depurativa, diurética, antiinflamatória, antiofídica e antirreumática, sendo as folhas a principal parte utilizada da planta, seja na forma de chá ou infusão. S. montevidensis não apresenta suas propriedades medicinais conhecidas e tendo uma grande variedade morfológica das folhas, isso leva a população a confundi-la com chapéu-de-couro . O presente estudo teve o objetivo de avaliar o potencial genotóxico e antiproliferativo de extratos das folhas secas de E. grandiflorus e S. montevidensis, sobre o ciclo celular de Allium cepa, bem como realizar análise por cromatografia líquida de alta eficiência (CLAE-DAD) para quantificação dos compostos dos extratos. O sistema teste A. cepa é bem aceito para a análise da genotoxicidade, sendo validado pelo Programa Internacional de Segurança Química (IPCS, OMS), podendo vir a alertar sobre possíveis danos que substâncias podem causar aos organismos a elas expostos. Para o teste foi coletado material vegetativo no habitat natural no Rio Grande do Sul, nos munícipios de: Santa Maria, São Sepé, Silveira Martins e Pinhal Grande e na forma comercializada de E. grandiflorus, separados em 2 grupos experimentais. Os extratos preparados, por infusão de 15 min. das folhas secas, em duas concentrações 6g. L-1 e 24g.L-1, para cada espécie e 2 controles, água (controle negativo) e o glifosato 15% (controle positivo). A análise cromatográfica (HPLC-DAD) revelou a presença de flavonóides e ácidos fenólicos para ambas as espécies. Sendo, maiores teores de ácido cafeico e flavonóide glicosídeo em E. grandiflorus e glicosídeo fenol em S. montevidensis. Este estudo mostrou que os extratos de E. grandiflorus e S. montevidensis apresentam potencial genotóxico e quando usadas em elevadas concentrações, potencial antiproliferativo.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em AgrobiologiaUFSMBRCiências BiológicasPlantas medicinaisGenotoxicidadeAntimutagênicoFlavonóidesChapéu-de-couroFalso chapéu-de-couroAllium cepaMedicinal plantsGenotoxicityAntimutagenicFlavonoidsLeather hatFalse leather hatAllium cepaCNPQ::CIENCIAS BIOLOGICASPotencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae)Potential genotoxicity and antiproliferative of extracts Echinodorus grandiflorus and Sagittaria montevidensis (Alismataceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTedesco, Solange Bosiohttp://lattes.cnpq.br/2541303074261787Silva, Antonio Carlos Ferreira dahttp://lattes.cnpq.br/9520096764579199Conterato, Ionara Fatimahttp://lattes.cnpq.br/3873651893060825http://lattes.cnpq.br/0864045856382054Coelho, Ana Paula Durand2000000000064005003005003006090974d-4c73-4bec-8e07-8cf0a1e0c33e9e7d23a5-882b-4a66-aa06-044e6777a79ea9badb65-5fab-4e20-b834-002be3e8ad36dd45dc90-90ff-4e57-9e6d-2e76cdf36c88info:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALCOELHO, ANA PAULA DURAND.pdfapplication/pdf864937http://repositorio.ufsm.br/bitstream/1/4859/1/COELHO%2c%20ANA%20PAULA%20DURAND.pdfa5ebbac18ed99d30aa43f3e5816020e2MD51TEXTCOELHO, ANA PAULA DURAND.pdf.txtCOELHO, ANA PAULA DURAND.pdf.txtExtracted texttext/plain104684http://repositorio.ufsm.br/bitstream/1/4859/2/COELHO%2c%20ANA%20PAULA%20DURAND.pdf.txtb2148e705197cb2ae25fd581a378a051MD52THUMBNAILCOELHO, ANA PAULA DURAND.pdf.jpgCOELHO, ANA PAULA DURAND.pdf.jpgIM Thumbnailimage/jpeg4932http://repositorio.ufsm.br/bitstream/1/4859/3/COELHO%2c%20ANA%20PAULA%20DURAND.pdf.jpgec9bd457b9e7ebfa3d944072f39923b1MD531/48592022-01-03 12:02:24.241oai:repositorio.ufsm.br:1/4859Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132022-01-03T15:02:24Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) |
dc.title.alternative.eng.fl_str_mv |
Potential genotoxicity and antiproliferative of extracts Echinodorus grandiflorus and Sagittaria montevidensis (Alismataceae) |
title |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) |
spellingShingle |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) Coelho, Ana Paula Durand Plantas medicinais Genotoxicidade Antimutagênico Flavonóides Chapéu-de-couro Falso chapéu-de-couro Allium cepa Medicinal plants Genotoxicity Antimutagenic Flavonoids Leather hat False leather hat Allium cepa CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) |
title_full |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) |
title_fullStr |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) |
title_full_unstemmed |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) |
title_sort |
Potencial genotóxico e antiproliferativo dos extratos de Echinodorus grandiflorus e Sagittaria montevidensis (Alismataceae) |
author |
Coelho, Ana Paula Durand |
author_facet |
Coelho, Ana Paula Durand |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Tedesco, Solange Bosio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2541303074261787 |
dc.contributor.referee1.fl_str_mv |
Silva, Antonio Carlos Ferreira da |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9520096764579199 |
dc.contributor.referee2.fl_str_mv |
Conterato, Ionara Fatima |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3873651893060825 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0864045856382054 |
dc.contributor.author.fl_str_mv |
Coelho, Ana Paula Durand |
contributor_str_mv |
Tedesco, Solange Bosio Silva, Antonio Carlos Ferreira da Conterato, Ionara Fatima |
dc.subject.por.fl_str_mv |
Plantas medicinais Genotoxicidade Antimutagênico Flavonóides Chapéu-de-couro Falso chapéu-de-couro Allium cepa |
topic |
Plantas medicinais Genotoxicidade Antimutagênico Flavonóides Chapéu-de-couro Falso chapéu-de-couro Allium cepa Medicinal plants Genotoxicity Antimutagenic Flavonoids Leather hat False leather hat Allium cepa CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Medicinal plants Genotoxicity Antimutagenic Flavonoids Leather hat False leather hat Allium cepa |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
description |
In Brazil, the economic potential of the germplasm of medicinal species is a treasure to be preserved and utilized, increasingly becoming a form of alternative therapy available for the population to use. However, the indiscriminate and uncontrolled use can, cause more harm than benefits to health and it is important to understand these plants, from cellular levels and the action on living organisms. The medicinal species Echinodorus grandiflorus and Sagittaria montevidensis belong to the family Alismataceae, known respectively as leather hat and false leather hat in Brazil. For E. grandiflorus the attributed medicinal potentials are depurative, diuretic, anti-inflammatory, antiophidic and anti-rheumatic action, were leaves are the main part of the plant used, either as a tea or infusion. S. montevidensis has no known medicinal properties and having a wide range of leaf morphologies, leading to population confused with leather hat . The present study evaluated the genotoxic and antiproliferative extracts of the dried leaves of E. grandiflorus and S. montevidensis using the cell cycle of Allium cepa, and performing analysis by high performance liquid chromatography (HPLC-DAD) for the quantification of the extracts of the compounds. The test system is well suited for the analysis of genotoxicity, being validated by the International Programme on Chemical Safety (IPCS, WHO) and can alert on the possible damages that can be caused to organisms exposed to the substances in the plants. For the test, plant material was collected in the field in Rio Grande do Sul, in the municipalities of: Santa Maria, São Sepé, Silveira Martins and Pinhal Grande and in the commercial form of E. grandiflorus, separated into two experimental groups. The extracts were prepared, by infusing dry leaves for 15 min., at two concentrations, 6g.L-1 e 24g.L-1, and two controls, water (negative control) and glyphosate 15% (positive control). The chromatographic analysis (HPLC-DAD), revealed the presence of flavonoids and phenolic acids for both species, higher concentrations of caffeic acid and flavonoid glycoside in E. grandiflorus and phenol glycoside in S. montevidensis. This study showed that extracts of E. grandiflorus and S. montevidensis show genotoxic potential and when used in high concentrations, antiproliferative potential. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-02-18 |
dc.date.accessioned.fl_str_mv |
2014-02-13 |
dc.date.available.fl_str_mv |
2014-02-13 |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
COELHO, Ana Paula Durand. Potential genotoxicity and antiproliferative of extracts Echinodorus grandiflorus and Sagittaria montevidensis (Alismataceae). 2013. 58 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4859 |
identifier_str_mv |
COELHO, Ana Paula Durand. Potential genotoxicity and antiproliferative of extracts Echinodorus grandiflorus and Sagittaria montevidensis (Alismataceae). 2013. 58 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
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http://repositorio.ufsm.br/handle/1/4859 |
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