Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/27546 |
Resumo: | Eugenia involucrata DC. is an underexplored native plant species, found in the Southern and Southeastern regions of Brazil. Recent studies report the presence of large amounts of phenolic compounds that potentially benefit human health in different parts (leaves, pulp, and seeds) of this species. Diabetes is characterized by hyperglycemia, which results in the glycation of proteins and excessive generation of reactive oxygen species (ROS) that lead to oxidative stress and the formation of advanced glycation end products (AGEs). Thus, one of the strategies for the treatment and/or protection of diabetes is to promote the reduction of blood glucose levels, through the inhibition of enzymes involved in carbohydrate digestion. So far, no study has been found in the literature on the effects of extracts from the pulp and seed E. involucrata towards enzymes involved in the digestion of carbohydrates and lipids. Therefore, the aim of this study was to investigate in vitro the anti-hyperglycemic and anti-obesogenic potential of phenolic compounds of ethanolic extracts from E. involucrata pulp and seed, through their ability to inhibit enzymes involved in the digestion of carbohydrates and lipids, and to evaluate the inhibition of AGEs formation, and the potential ability to neutralize reactive species of biological importance. The seed extract had higher content of total phenolic compounds (11,550.5 ± 633.0 µg. mL-1 ), being composed of phenolic acids and flavonoids, than the pulp extract (440.7 ± 28.1 µg. mL-1 ) that was composed of phenolic acids, flavonoids and anthocyanins. The IC50 values (µg of phenolic compounds. mL-1 ) revealed that the extracts had higher potency than acarbose towards the inhibition of α-amylase (0.2 ± 0.0 for pulp; 1.3 ± 0.2 for seed; 39.9 ± 3.9 for acarbose) and α-glucosidase (0.3 ± 0.0 for pulp; 1.5 ± 0.0 for seed; 2,019.0 ± 81.3 for acarbose), but lower potency than orlistat to inhibit pancreatic lipase (0.7 ± 0.0 for pulp; 0.6 ± 0.0 for seed; 0.1 ± 0.0 for orlistat). Extracts also inhibited (IC50 values as µg of phenolic compounds. mL-1 ) the formation of AGEs (6.8 ± 0.3 for pulp; 30.9 ± 1.7 for seed; 256.6 ± 13.7 for aminoguanidine) and were able to remove free radical species of biological relevance, namely peroxyl radical (0.02 ± 0.0 for pulp; 0.12 ± 0.0 for seed), hydroxyl radical (3.9 ± 0.0 for pulp; 4.5 ± 0.1 for seed). Despite the higher potency of phenolic compounds extracted from E. involucrata pulp towards all biological effects investigated when compared to the phenolics from seed extract, seed extraction has greater yield. Therefore, the concentration of phenolic compounds was 26 times higher in seed than pulp extract and seed extract had higher potency than pulp extract when taking into account the amount of pulp or seed that is necessary to obtain each extract. The results obtained in this study provide a basis for further studies in animal models and clinical studies for the development of new drugs or nutraceuticals, as well as food products with functional properties. |
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2023-01-10T14:57:09Z2023-01-10T14:57:09Z2022-11-29http://repositorio.ufsm.br/handle/1/27546Eugenia involucrata DC. is an underexplored native plant species, found in the Southern and Southeastern regions of Brazil. Recent studies report the presence of large amounts of phenolic compounds that potentially benefit human health in different parts (leaves, pulp, and seeds) of this species. Diabetes is characterized by hyperglycemia, which results in the glycation of proteins and excessive generation of reactive oxygen species (ROS) that lead to oxidative stress and the formation of advanced glycation end products (AGEs). Thus, one of the strategies for the treatment and/or protection of diabetes is to promote the reduction of blood glucose levels, through the inhibition of enzymes involved in carbohydrate digestion. So far, no study has been found in the literature on the effects of extracts from the pulp and seed E. involucrata towards enzymes involved in the digestion of carbohydrates and lipids. Therefore, the aim of this study was to investigate in vitro the anti-hyperglycemic and anti-obesogenic potential of phenolic compounds of ethanolic extracts from E. involucrata pulp and seed, through their ability to inhibit enzymes involved in the digestion of carbohydrates and lipids, and to evaluate the inhibition of AGEs formation, and the potential ability to neutralize reactive species of biological importance. The seed extract had higher content of total phenolic compounds (11,550.5 ± 633.0 µg. mL-1 ), being composed of phenolic acids and flavonoids, than the pulp extract (440.7 ± 28.1 µg. mL-1 ) that was composed of phenolic acids, flavonoids and anthocyanins. The IC50 values (µg of phenolic compounds. mL-1 ) revealed that the extracts had higher potency than acarbose towards the inhibition of α-amylase (0.2 ± 0.0 for pulp; 1.3 ± 0.2 for seed; 39.9 ± 3.9 for acarbose) and α-glucosidase (0.3 ± 0.0 for pulp; 1.5 ± 0.0 for seed; 2,019.0 ± 81.3 for acarbose), but lower potency than orlistat to inhibit pancreatic lipase (0.7 ± 0.0 for pulp; 0.6 ± 0.0 for seed; 0.1 ± 0.0 for orlistat). Extracts also inhibited (IC50 values as µg of phenolic compounds. mL-1 ) the formation of AGEs (6.8 ± 0.3 for pulp; 30.9 ± 1.7 for seed; 256.6 ± 13.7 for aminoguanidine) and were able to remove free radical species of biological relevance, namely peroxyl radical (0.02 ± 0.0 for pulp; 0.12 ± 0.0 for seed), hydroxyl radical (3.9 ± 0.0 for pulp; 4.5 ± 0.1 for seed). Despite the higher potency of phenolic compounds extracted from E. involucrata pulp towards all biological effects investigated when compared to the phenolics from seed extract, seed extraction has greater yield. Therefore, the concentration of phenolic compounds was 26 times higher in seed than pulp extract and seed extract had higher potency than pulp extract when taking into account the amount of pulp or seed that is necessary to obtain each extract. The results obtained in this study provide a basis for further studies in animal models and clinical studies for the development of new drugs or nutraceuticals, as well as food products with functional properties.A Eugenia involucrata DC. é uma espécie vegetal nativa subexplorada, presente nas regiões sul e sudeste do Brasil. Estudos recentes relatam a presença de grandes quantidades de compostos fenólicos potencialmente benéficos para a saúde humana em diferentes partes (folhas, polpa e sementes) desta espécie. O diabetes é uma doença caracterizada pela hiperglicemia, a qual resulta na glicação de proteínas e geração excessiva de espécies reativas de oxigênio (EROs) que leva ao estresse oxidativo e a formação de produtos finais de glicação avançada (AGEs), ambos envolvidos na patogênese da doença. Dessa forma, uma das estratégias de tratamento e/ou prevenção do diabetes é promover a redução dos níveis de glicose sanguínea, através da inibição de enzimas envolvidas no metabolismo de carboidratos e lipídeos. Até o presente momento não foi encontrado na literatura cientifica nenhum estudo realizado com extratos da polpa e semente de E. involucrata na inibição de enzimas envolvidas na digestão de carboidratos e lipídeos. Sendo assim, o objetivo deste estudo foi investigar in vitro o potencial anti-hiperglicêmico e anti-obesogênico de extratos etanólicos de polpa e semente de E. involucrata, por meio de sua capacidade de inibir enzimas envolvidas na digestão de carboidratos e lipídios, e avaliar a inibição da formação de AGEs, e a capacidade de neutralizar espécies reativas de importância biológica. O extrato de semente de E. involucrata apresentou maior teor compostos fenólicos totais (11.550,5 ± 633,0 µg. mL-1 ), compostos por ácidos fenólicos e flavonóides, que o extrato da polpa da fruta (440,7 ± 28,1 µg. mL-1 ), composto por ácidos fenólicos, flavonóides e antocianinas. Os valores de IC50 (expressos como µg de compostos fenólicos. mL-1 ) revelaram que os compostos fenólicos dos extratos de E. involucrata apresentaram potência superior a da acarbose para inibir as enzimas digestivas αamilase (IC50 polpa: 0,2 ± 0,0 µg. mL-1 ; IC50 semente: 1,3 ± 0,2 µg. mL-1 ; acarbose: 39,9 ± 3,9 µg. mL-1 ), α-glicosidase (IC50 polpa: 0,3± 0,0 µg. mL-1 ; IC50 semente: 1,5 ± 0,0 µg. mL-1 ; acarbose: 2.019,0 ± 81,3 µg. mL-1 ), mas inferior a do orlistat para inibir a lipase pancreática (IC50 polpa: 0,7 ± 0,0 µg. mL-1 ; IC50 semente: 0,6 ± 0,0 µg. mL-1 ; orlistat: 0,1 ± 0,0 µg. mL-1 ). Também inibiram a formação de AGEs (IC50 polpa: 6,8 ± 0,3 µg. mL-1 ; IC50 semente: 30,9 ± µg. mL-1 ), e foram capazes de remover espécies radicalares de importância biológica (radical peroxil: IC50 polpa: 0,02 ± 0,0 µg. mL-1 , IC50 semente: 0,12 ± 0,0 µg. mL-1 ; e radical hidroxil: IC50 polpa: 3,9 ± 0,0 µg. mL-1 , IC50 semente: 4,5 ± 0,1 µg. mL-1 ). Apesar dos compostos fenólicos extraídos da polpa da E. involucrata terem apresentado maior potência na inibição de enzimas digestivas e remoção de espécies reativas em comparação com fenólicos da semente da fruta, a extração da semente apresenta maior rendimento, resultando em concentração de fenólicos 26 vezes superior à do extrato da polpa, e maior potência do extrato de semente quando considerada a quantidade de polpa ou semente necessária para obtenção dos extratos. Os resultados obtidos neste estudo fornecem a base para estudos futuros em modelos in vivo e estudos clínicos visando a formulação de novos fármacos ou nutracêuticos, bem como de produtos alimentícios com propriedades funcionais.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Ciência e Tecnologia dos AlimentosUFSMBrasilCiência e Tecnologia dos AlimentosAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCereja-do-rio-grandeCompostos bioativosDiabetesEnzimas digestivasProdutos finais de glicação avançadaCherry-of-rio-grandeBioactive compoundsDigestive enzymesAdvanced glycation end productsCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSExtratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitroExtracts of cherry-of-rio-grande (Eugenia involucrata): phytochemical characterization and evaluation of bioactivity in in vitro modelsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEmanuelli, Tatianahttp://lattes.cnpq.br/2165391096880394Somacal, Sabrinac40d429f-10a0-4b95-9608-dc4dfd0092c9600Conterato, Greicy Michelle MarafigaSerra, Ana TeresaLozano-Sánchez, Jesúshttp://lattes.cnpq.br/3694035611521877Smaniotto, Franciele Aline5007000000066006006006006006007812e191-0e91-4b97-bc2b-b442502f01865de2aa75-7669-4dcb-8d43-4b83ebcfc28cdb78b1f3-a3f4-4c5d-8d55-a95d19e8eaf2077c671e-ea24-458d-a3e2-bc34906fc14104086808-56c4-424a-a7cd-f0d3cea361cfreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCTA_2022_SMANIOTTO_FRANCIELE.pdfDIS_PPGCTA_2022_SMANIOTTO_FRANCIELE.pdfDissertação de Mestradoapplication/pdf1521203http://repositorio.ufsm.br/bitstream/1/27546/1/DIS_PPGCTA_2022_SMANIOTTO_FRANCIELE.pdf09b9d6f8faf310b7ba21bad386382cb2MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro |
dc.title.alternative.eng.fl_str_mv |
Extracts of cherry-of-rio-grande (Eugenia involucrata): phytochemical characterization and evaluation of bioactivity in in vitro models |
title |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro |
spellingShingle |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro Smaniotto, Franciele Aline Cereja-do-rio-grande Compostos bioativos Diabetes Enzimas digestivas Produtos finais de glicação avançada Cherry-of-rio-grande Bioactive compounds Digestive enzymes Advanced glycation end products CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
title_short |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro |
title_full |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro |
title_fullStr |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro |
title_full_unstemmed |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro |
title_sort |
Extratos de cerejeira-do-rio-grande (Eugenia involucrata): avaliação da bioatividade em modelos in vitro |
author |
Smaniotto, Franciele Aline |
author_facet |
Smaniotto, Franciele Aline |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Emanuelli, Tatiana |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2165391096880394 |
dc.contributor.advisor-co1.fl_str_mv |
Somacal, Sabrina |
dc.contributor.advisor-co2.fl_str_mv |
Conterato, Greicy Michelle Marafiga |
dc.contributor.referee1.fl_str_mv |
Serra, Ana Teresa |
dc.contributor.referee2.fl_str_mv |
Lozano-Sánchez, Jesús |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3694035611521877 |
dc.contributor.author.fl_str_mv |
Smaniotto, Franciele Aline |
contributor_str_mv |
Emanuelli, Tatiana Somacal, Sabrina Conterato, Greicy Michelle Marafiga Serra, Ana Teresa Lozano-Sánchez, Jesús |
dc.subject.por.fl_str_mv |
Cereja-do-rio-grande Compostos bioativos Diabetes Enzimas digestivas Produtos finais de glicação avançada |
topic |
Cereja-do-rio-grande Compostos bioativos Diabetes Enzimas digestivas Produtos finais de glicação avançada Cherry-of-rio-grande Bioactive compounds Digestive enzymes Advanced glycation end products CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Cherry-of-rio-grande Bioactive compounds Digestive enzymes Advanced glycation end products |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
description |
Eugenia involucrata DC. is an underexplored native plant species, found in the Southern and Southeastern regions of Brazil. Recent studies report the presence of large amounts of phenolic compounds that potentially benefit human health in different parts (leaves, pulp, and seeds) of this species. Diabetes is characterized by hyperglycemia, which results in the glycation of proteins and excessive generation of reactive oxygen species (ROS) that lead to oxidative stress and the formation of advanced glycation end products (AGEs). Thus, one of the strategies for the treatment and/or protection of diabetes is to promote the reduction of blood glucose levels, through the inhibition of enzymes involved in carbohydrate digestion. So far, no study has been found in the literature on the effects of extracts from the pulp and seed E. involucrata towards enzymes involved in the digestion of carbohydrates and lipids. Therefore, the aim of this study was to investigate in vitro the anti-hyperglycemic and anti-obesogenic potential of phenolic compounds of ethanolic extracts from E. involucrata pulp and seed, through their ability to inhibit enzymes involved in the digestion of carbohydrates and lipids, and to evaluate the inhibition of AGEs formation, and the potential ability to neutralize reactive species of biological importance. The seed extract had higher content of total phenolic compounds (11,550.5 ± 633.0 µg. mL-1 ), being composed of phenolic acids and flavonoids, than the pulp extract (440.7 ± 28.1 µg. mL-1 ) that was composed of phenolic acids, flavonoids and anthocyanins. The IC50 values (µg of phenolic compounds. mL-1 ) revealed that the extracts had higher potency than acarbose towards the inhibition of α-amylase (0.2 ± 0.0 for pulp; 1.3 ± 0.2 for seed; 39.9 ± 3.9 for acarbose) and α-glucosidase (0.3 ± 0.0 for pulp; 1.5 ± 0.0 for seed; 2,019.0 ± 81.3 for acarbose), but lower potency than orlistat to inhibit pancreatic lipase (0.7 ± 0.0 for pulp; 0.6 ± 0.0 for seed; 0.1 ± 0.0 for orlistat). Extracts also inhibited (IC50 values as µg of phenolic compounds. mL-1 ) the formation of AGEs (6.8 ± 0.3 for pulp; 30.9 ± 1.7 for seed; 256.6 ± 13.7 for aminoguanidine) and were able to remove free radical species of biological relevance, namely peroxyl radical (0.02 ± 0.0 for pulp; 0.12 ± 0.0 for seed), hydroxyl radical (3.9 ± 0.0 for pulp; 4.5 ± 0.1 for seed). Despite the higher potency of phenolic compounds extracted from E. involucrata pulp towards all biological effects investigated when compared to the phenolics from seed extract, seed extraction has greater yield. Therefore, the concentration of phenolic compounds was 26 times higher in seed than pulp extract and seed extract had higher potency than pulp extract when taking into account the amount of pulp or seed that is necessary to obtain each extract. The results obtained in this study provide a basis for further studies in animal models and clinical studies for the development of new drugs or nutraceuticals, as well as food products with functional properties. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-11-29 |
dc.date.accessioned.fl_str_mv |
2023-01-10T14:57:09Z |
dc.date.available.fl_str_mv |
2023-01-10T14:57:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/27546 |
url |
http://repositorio.ufsm.br/handle/1/27546 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500700000006 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 600 |
dc.relation.authority.fl_str_mv |
7812e191-0e91-4b97-bc2b-b442502f0186 5de2aa75-7669-4dcb-8d43-4b83ebcfc28c db78b1f3-a3f4-4c5d-8d55-a95d19e8eaf2 077c671e-ea24-458d-a3e2-bc34906fc141 04086808-56c4-424a-a7cd-f0d3cea361cf |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Ciência e Tecnologia dos Alimentos |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional Manancial UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Repositório Institucional Manancial UFSM |
collection |
Repositório Institucional Manancial UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/27546/1/DIS_PPGCTA_2022_SMANIOTTO_FRANCIELE.pdf http://repositorio.ufsm.br/bitstream/1/27546/2/license_rdf http://repositorio.ufsm.br/bitstream/1/27546/3/license.txt |
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09b9d6f8faf310b7ba21bad386382cb2 4460e5956bc1d1639be9ae6146a50347 2f0571ecee68693bd5cd3f17c1e075df |
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MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
ouvidoria@ufsm.br |
_version_ |
1808854724253843456 |