Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149 |
Resumo: | Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum activity in alkaline pH (8.5) and 50°C; being stable to the two variables at 120 minutes during the test. The clotting activity was directly proportional to the temperature at the better concentration of CaCl2 (10mmol L-1). The results indicate that enzyme is a possible replacement for calf rennet |
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Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leiteenzimas coagulantes do leiteatividade proteolíticacoagulante vegetalfruta-pãoBreadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum activity in alkaline pH (8.5) and 50°C; being stable to the two variables at 120 minutes during the test. The clotting activity was directly proportional to the temperature at the better concentration of CaCl2 (10mmol L-1). The results indicate that enzyme is a possible replacement for calf rennetUniversidade Federal de Santa Maria2015-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149Ciência Rural v.45 n.1 2015reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20140310info:eu-repo/semantics/openAccessSoares,Elisângela FrançaSilva,Anna Carolina daQueiroz,Alana Emilia Soares de FrançaGomes,José Erick GalindoHerculano,Polyanna NunesMoreira,Keila Aparecidapor2016-05-12T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite |
title |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite |
spellingShingle |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite Soares,Elisângela França enzimas coagulantes do leite atividade proteolítica coagulante vegetal fruta-pão |
title_short |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite |
title_full |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite |
title_fullStr |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite |
title_full_unstemmed |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite |
title_sort |
Potencial do latex da fruta pão (Artocarpus altilis) como agente coagulante do leite |
author |
Soares,Elisângela França |
author_facet |
Soares,Elisângela França Silva,Anna Carolina da Queiroz,Alana Emilia Soares de França Gomes,José Erick Galindo Herculano,Polyanna Nunes Moreira,Keila Aparecida |
author_role |
author |
author2 |
Silva,Anna Carolina da Queiroz,Alana Emilia Soares de França Gomes,José Erick Galindo Herculano,Polyanna Nunes Moreira,Keila Aparecida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Soares,Elisângela França Silva,Anna Carolina da Queiroz,Alana Emilia Soares de França Gomes,José Erick Galindo Herculano,Polyanna Nunes Moreira,Keila Aparecida |
dc.subject.por.fl_str_mv |
enzimas coagulantes do leite atividade proteolítica coagulante vegetal fruta-pão |
topic |
enzimas coagulantes do leite atividade proteolítica coagulante vegetal fruta-pão |
description |
Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum activity in alkaline pH (8.5) and 50°C; being stable to the two variables at 120 minutes during the test. The clotting activity was directly proportional to the temperature at the better concentration of CaCl2 (10mmol L-1). The results indicate that enzyme is a possible replacement for calf rennet |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20140310 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.45 n.1 2015 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140546449833984 |