Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs

• Autor(a) principal: Alves,Endrigo Gabellini Leonel
• Data de Publicação: 2017
• Outros Autores: Serakides,Rogéria, Rosado,Isabel Rodrigues, Paez,Omar Leonardo Aristizabal, Varon,Jéssica Alejandra Castro, Machado,Felipe Nemer, Fukushima,Fabíola Bono, Góes,Alfredo Miranda, Rezende,Cleuza Maria de Faria
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700604
• topic: bone regeneration; orthopedics; cellular therapy; fracture non-union

ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days.