Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700604 |
Resumo: | ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days. |
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Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogsbone regenerationorthopedicscellular therapyfracture non-unionABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days.Universidade Federal de Santa Maria2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700604Ciência Rural v.47 n.7 2017reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20151109info:eu-repo/semantics/openAccessAlves,Endrigo Gabellini LeonelSerakides,RogériaRosado,Isabel RodriguesPaez,Omar Leonardo AristizabalVaron,Jéssica Alejandra CastroMachado,Felipe NemerFukushima,Fabíola BonoGóes,Alfredo MirandaRezende,Cleuza Maria de Fariaeng2017-11-22T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs |
title |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs |
spellingShingle |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs Alves,Endrigo Gabellini Leonel bone regeneration orthopedics cellular therapy fracture non-union |
title_short |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs |
title_full |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs |
title_fullStr |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs |
title_full_unstemmed |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs |
title_sort |
Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs |
author |
Alves,Endrigo Gabellini Leonel |
author_facet |
Alves,Endrigo Gabellini Leonel Serakides,Rogéria Rosado,Isabel Rodrigues Paez,Omar Leonardo Aristizabal Varon,Jéssica Alejandra Castro Machado,Felipe Nemer Fukushima,Fabíola Bono Góes,Alfredo Miranda Rezende,Cleuza Maria de Faria |
author_role |
author |
author2 |
Serakides,Rogéria Rosado,Isabel Rodrigues Paez,Omar Leonardo Aristizabal Varon,Jéssica Alejandra Castro Machado,Felipe Nemer Fukushima,Fabíola Bono Góes,Alfredo Miranda Rezende,Cleuza Maria de Faria |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves,Endrigo Gabellini Leonel Serakides,Rogéria Rosado,Isabel Rodrigues Paez,Omar Leonardo Aristizabal Varon,Jéssica Alejandra Castro Machado,Felipe Nemer Fukushima,Fabíola Bono Góes,Alfredo Miranda Rezende,Cleuza Maria de Faria |
dc.subject.por.fl_str_mv |
bone regeneration orthopedics cellular therapy fracture non-union |
topic |
bone regeneration orthopedics cellular therapy fracture non-union |
description |
ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700604 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700604 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20151109 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.47 n.7 2017 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140551644479488 |