Proline synthesis and physiological response of cassava genotypes under in vitro salinity
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600401 |
Resumo: | ABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design was completely randomized in double factorial scheme (3 genotypes x 5 salt treatments), with seven repetitions for growth variables. For comparing proline content, completely randomized design was used in a plot subdivided in time, with genotype and NaCl factors in plot and time in subplot, with two repetitions. For r time and genotypes Tukey test (P<0,05) was used and for NaCl levels regression test (P<0,05). Salinity affected the growth of all varieties; although, BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress. There was an increase in the accumulation of proline from the salt increment, this synthesis of proline being a biochemical indicator of salt stress in cassava plants cultivated in vitro. |
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Proline synthesis and physiological response of cassava genotypes under in vitro salinityManihot esculentaabiotic stressosmoprotectorsABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design was completely randomized in double factorial scheme (3 genotypes x 5 salt treatments), with seven repetitions for growth variables. For comparing proline content, completely randomized design was used in a plot subdivided in time, with genotype and NaCl factors in plot and time in subplot, with two repetitions. For r time and genotypes Tukey test (P<0,05) was used and for NaCl levels regression test (P<0,05). Salinity affected the growth of all varieties; although, BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress. There was an increase in the accumulation of proline from the salt increment, this synthesis of proline being a biochemical indicator of salt stress in cassava plants cultivated in vitro.Universidade Federal de Santa Maria2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600401Ciência Rural v.49 n.6 2019reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20170175info:eu-repo/semantics/openAccessCardoso,Milena NascimentoAraújo,Aparecida Gomes deOliveira,Leila Albuquerque ResendeCardoso,Bruno TrindadeMuniz,Ana Veruska Cruz da SilvaSantos,Paulo Sérgio Neves dosOliveira,Annie Carolina Araújo deMachado,Caroline de AraújoLedo,Ana da Silvaeng2019-05-13T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity |
title |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity |
spellingShingle |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity Cardoso,Milena Nascimento Manihot esculenta abiotic stress osmoprotectors |
title_short |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity |
title_full |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity |
title_fullStr |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity |
title_full_unstemmed |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity |
title_sort |
Proline synthesis and physiological response of cassava genotypes under in vitro salinity |
author |
Cardoso,Milena Nascimento |
author_facet |
Cardoso,Milena Nascimento Araújo,Aparecida Gomes de Oliveira,Leila Albuquerque Resende Cardoso,Bruno Trindade Muniz,Ana Veruska Cruz da Silva Santos,Paulo Sérgio Neves dos Oliveira,Annie Carolina Araújo de Machado,Caroline de Araújo Ledo,Ana da Silva |
author_role |
author |
author2 |
Araújo,Aparecida Gomes de Oliveira,Leila Albuquerque Resende Cardoso,Bruno Trindade Muniz,Ana Veruska Cruz da Silva Santos,Paulo Sérgio Neves dos Oliveira,Annie Carolina Araújo de Machado,Caroline de Araújo Ledo,Ana da Silva |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cardoso,Milena Nascimento Araújo,Aparecida Gomes de Oliveira,Leila Albuquerque Resende Cardoso,Bruno Trindade Muniz,Ana Veruska Cruz da Silva Santos,Paulo Sérgio Neves dos Oliveira,Annie Carolina Araújo de Machado,Caroline de Araújo Ledo,Ana da Silva |
dc.subject.por.fl_str_mv |
Manihot esculenta abiotic stress osmoprotectors |
topic |
Manihot esculenta abiotic stress osmoprotectors |
description |
ABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design was completely randomized in double factorial scheme (3 genotypes x 5 salt treatments), with seven repetitions for growth variables. For comparing proline content, completely randomized design was used in a plot subdivided in time, with genotype and NaCl factors in plot and time in subplot, with two repetitions. For r time and genotypes Tukey test (P<0,05) was used and for NaCl levels regression test (P<0,05). Salinity affected the growth of all varieties; although, BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress. There was an increase in the accumulation of proline from the salt increment, this synthesis of proline being a biochemical indicator of salt stress in cassava plants cultivated in vitro. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600401 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600401 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20170175 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.49 n.6 2019 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140553858023424 |