Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes

Detalhes bibliográficos
Autor(a) principal: Ely,Valessa Lunkes
Data de Publicação: 2019
Outros Autores: Gressler,Letícia Trevisan, Sutili,Fernando Jonas, Ribeiro,Márcio Garcia, Costa,Mateus Matiuzzi da, Vargas,Agueda Castagna de, Botton,Sônia de Avila
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200451
Resumo: ABSTRACT: Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
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spelling Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyesPrototheca zopfii, dairy cattlebovine mastitis, intramammary infections, microplate biofilm assay, virulence factorABSTRACT: Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.Universidade Federal de Santa Maria2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200451Ciência Rural v.49 n.2 2019reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20180574info:eu-repo/semantics/openAccessEly,Valessa LunkesGressler,Letícia TrevisanSutili,Fernando JonasRibeiro,Márcio GarciaCosta,Mateus Matiuzzi daVargas,Agueda Castagna deBotton,Sônia de Avilaeng2019-02-25T00:00:00ZRevista
dc.title.none.fl_str_mv Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
spellingShingle Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
Ely,Valessa Lunkes
Prototheca zopfii, dairy cattle
bovine mastitis, intramammary infections, microplate biofilm assay, virulence factor
title_short Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_full Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_fullStr Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_full_unstemmed Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_sort Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
author Ely,Valessa Lunkes
author_facet Ely,Valessa Lunkes
Gressler,Letícia Trevisan
Sutili,Fernando Jonas
Ribeiro,Márcio Garcia
Costa,Mateus Matiuzzi da
Vargas,Agueda Castagna de
Botton,Sônia de Avila
author_role author
author2 Gressler,Letícia Trevisan
Sutili,Fernando Jonas
Ribeiro,Márcio Garcia
Costa,Mateus Matiuzzi da
Vargas,Agueda Castagna de
Botton,Sônia de Avila
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ely,Valessa Lunkes
Gressler,Letícia Trevisan
Sutili,Fernando Jonas
Ribeiro,Márcio Garcia
Costa,Mateus Matiuzzi da
Vargas,Agueda Castagna de
Botton,Sônia de Avila
dc.subject.por.fl_str_mv Prototheca zopfii, dairy cattle
bovine mastitis, intramammary infections, microplate biofilm assay, virulence factor
topic Prototheca zopfii, dairy cattle
bovine mastitis, intramammary infections, microplate biofilm assay, virulence factor
description ABSTRACT: Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200451
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200451
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20180574
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.49 n.2 2019
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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