Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800404 |
Resumo: | ABSTRACT: One of the significant obstacles to the growth of sugarcane production is the infection by phytopathogens, mainly by the bacterium Leifsonia xyli subsp. xyli (Lxx) causal agent of Ratoon stunting disease. Thus, this research aimed to evaluate the effects of kasugamycin on the in vitro growth of sugarcane, as well as its effect on the bacterium Lxx. Explants of strain SP791011 from sugarcane were inoculated in MS culture medium supplemented with the antimicrobial kasugamycin at concentrations of 0.00; 0.87; 1.08; 1.74 and 3.48 mL.L-1, where they remained for 30 days. After this period, the survival rate, shoot number per explant, height of the explants, phytomass, dry phytomass and phytosanitary were evaluated based on the presence of genomic DNA of Lxx. It was verified that the culture in kasugamycin influenced the morphological variables negatively; nevertheless, the antimicrobial did not demonstrate phytotoxicity to the plants. All treatments tested in this experiment were diagnosed as positive, with DNA amplification for Lxx, despite it was observed a reduction in bacterial load, suggesting that kasugamycin at higher doses can be evaluated as an attempt to eliminate the bacterium in the in vitro cultivation of sugarcane. |
id |
UFSM-2_b167950425f07100c266e77addd9616c |
---|---|
oai_identifier_str |
oai:scielo:S0103-84782019000800404 |
network_acronym_str |
UFSM-2 |
network_name_str |
Ciência rural (Online) |
repository_id_str |
|
spelling |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcanemicropropagationendophyticRatoon stunting diseaseABSTRACT: One of the significant obstacles to the growth of sugarcane production is the infection by phytopathogens, mainly by the bacterium Leifsonia xyli subsp. xyli (Lxx) causal agent of Ratoon stunting disease. Thus, this research aimed to evaluate the effects of kasugamycin on the in vitro growth of sugarcane, as well as its effect on the bacterium Lxx. Explants of strain SP791011 from sugarcane were inoculated in MS culture medium supplemented with the antimicrobial kasugamycin at concentrations of 0.00; 0.87; 1.08; 1.74 and 3.48 mL.L-1, where they remained for 30 days. After this period, the survival rate, shoot number per explant, height of the explants, phytomass, dry phytomass and phytosanitary were evaluated based on the presence of genomic DNA of Lxx. It was verified that the culture in kasugamycin influenced the morphological variables negatively; nevertheless, the antimicrobial did not demonstrate phytotoxicity to the plants. All treatments tested in this experiment were diagnosed as positive, with DNA amplification for Lxx, despite it was observed a reduction in bacterial load, suggesting that kasugamycin at higher doses can be evaluated as an attempt to eliminate the bacterium in the in vitro cultivation of sugarcane.Universidade Federal de Santa Maria2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800404Ciência Rural v.49 n.8 2019reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20181009info:eu-repo/semantics/openAccessGarcia,Fabio RibeiroCavalcante,Felipe Lira de SáSouza,Robson Antonio deHoullou,Laureen Michelleeng2019-08-20T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane |
title |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane |
spellingShingle |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane Garcia,Fabio Ribeiro micropropagation endophytic Ratoon stunting disease |
title_short |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane |
title_full |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane |
title_fullStr |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane |
title_full_unstemmed |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane |
title_sort |
Kasugamycin on Leifsonia xyli subsp. xyli in the in vitro culture of sugarcane |
author |
Garcia,Fabio Ribeiro |
author_facet |
Garcia,Fabio Ribeiro Cavalcante,Felipe Lira de Sá Souza,Robson Antonio de Houllou,Laureen Michelle |
author_role |
author |
author2 |
Cavalcante,Felipe Lira de Sá Souza,Robson Antonio de Houllou,Laureen Michelle |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Garcia,Fabio Ribeiro Cavalcante,Felipe Lira de Sá Souza,Robson Antonio de Houllou,Laureen Michelle |
dc.subject.por.fl_str_mv |
micropropagation endophytic Ratoon stunting disease |
topic |
micropropagation endophytic Ratoon stunting disease |
description |
ABSTRACT: One of the significant obstacles to the growth of sugarcane production is the infection by phytopathogens, mainly by the bacterium Leifsonia xyli subsp. xyli (Lxx) causal agent of Ratoon stunting disease. Thus, this research aimed to evaluate the effects of kasugamycin on the in vitro growth of sugarcane, as well as its effect on the bacterium Lxx. Explants of strain SP791011 from sugarcane were inoculated in MS culture medium supplemented with the antimicrobial kasugamycin at concentrations of 0.00; 0.87; 1.08; 1.74 and 3.48 mL.L-1, where they remained for 30 days. After this period, the survival rate, shoot number per explant, height of the explants, phytomass, dry phytomass and phytosanitary were evaluated based on the presence of genomic DNA of Lxx. It was verified that the culture in kasugamycin influenced the morphological variables negatively; nevertheless, the antimicrobial did not demonstrate phytotoxicity to the plants. All treatments tested in this experiment were diagnosed as positive, with DNA amplification for Lxx, despite it was observed a reduction in bacterial load, suggesting that kasugamycin at higher doses can be evaluated as an attempt to eliminate the bacterium in the in vitro cultivation of sugarcane. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800404 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800404 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20181009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.49 n.8 2019 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140553923035136 |