Chemical profile, anti 5-lipoxygenase and cyclooxegenase inhibitory effects of ginger (Zingiber officinale) rhizome, callus and callus treated with elicitors

• Autor(a) principal: Ali,Ammar Mohammed Ahmed
• Data de Publicação: 2022
• Outros Autores: El-Nour,Mawahib ElAmin Mohamed, Al-Atar,Abdulrahman Abdullah, Mohammad,Owais, El-Sheikh,Mohamed Abdel-Raouf, Qahtan,Ahmed Ali, Abdel-Salam,Eslam Mohamed, Yagi,Sakina Mohamed
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000401
• topic: ginger; anti-5-lipoxygenase activity; yeast extract; salicylic acid; glycine

ABSTRACT: The present study investigated the chemical profiles and evaluated the inhibitory effect against 5-Lipoxygenase (5-Lox) activity for extracts of ginger rhizome, callus, and callus treated with the elicitors; yeast extract (100, 300 and 500 mg/L), glycine (100, 200 and 300 mg/L) and salicylic acid (100 and 200 mg/L). Oils and chloroform: methanol (CM) extracts were prepared by maceration in petroleum ether and CM (1:1, v/v), respectively. Chemical profiles were determined by gas chromatography/mass spectrometry (GC/MS) analysis. Oil of the callus recorded higher 5-Lox inhibitory effect (IC50 58.33±4.66 μg/mL) than the oil of rhizome (IC50168.34±15.64 μg/mL) and comparable to that of the positive control; Nordihydroguaiaretic acid (IC50 61.25±1.02 μg/mL). The chemical profile of the callus oil contained large amounts of fatty acids, mainly the unsaturated fatty acid oleic acid (31.11%) and saturated fatty acid palmitic acid (28.56%). Elicitors modified the chemical profile of the callus and ameliorated the anti-5-Lox activity of CM extract of the callus. CM extracts of callus treated with 100 and 300 mg/L yeast extract and 50 mg/L salicylic acid significantly suppressed (P ≤ 0.05) the 5-Lox activity by 33.16%, 25.46% and 16%, respectively as compared to the CM extract of untreated callus. In conclusion, ginger callus could be considered as a valuable dietary supplement in the treatment of various inflammatory disorders.