Cross-amplification of heterologous microsatellite markers in Piracanjuba
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402 |
Resumo: | ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species. |
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Cross-amplification of heterologous microsatellite markers in PiracanjubaBrycon orbignyanusconservationmolecular markersheterologous primersABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.Universidade Federal de Santa Maria2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402Ciência Rural v.47 n.12 2017reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20170374info:eu-repo/semantics/openAccessCastro,Pedro Luiz deRibeiro,Ricardo PereiraSantos,Silvio Carlos Alves dosGoes,Elenice Souza dos ReisSouza,Felipe Pinheiro dePoveda-Parra,Angela RocioVargas,LauroUrrea-Rojas,Angela MariaLopera-Barrero,Nelson Mauricioeng2017-11-28T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Cross-amplification of heterologous microsatellite markers in Piracanjuba |
title |
Cross-amplification of heterologous microsatellite markers in Piracanjuba |
spellingShingle |
Cross-amplification of heterologous microsatellite markers in Piracanjuba Castro,Pedro Luiz de Brycon orbignyanus conservation molecular markers heterologous primers |
title_short |
Cross-amplification of heterologous microsatellite markers in Piracanjuba |
title_full |
Cross-amplification of heterologous microsatellite markers in Piracanjuba |
title_fullStr |
Cross-amplification of heterologous microsatellite markers in Piracanjuba |
title_full_unstemmed |
Cross-amplification of heterologous microsatellite markers in Piracanjuba |
title_sort |
Cross-amplification of heterologous microsatellite markers in Piracanjuba |
author |
Castro,Pedro Luiz de |
author_facet |
Castro,Pedro Luiz de Ribeiro,Ricardo Pereira Santos,Silvio Carlos Alves dos Goes,Elenice Souza dos Reis Souza,Felipe Pinheiro de Poveda-Parra,Angela Rocio Vargas,Lauro Urrea-Rojas,Angela Maria Lopera-Barrero,Nelson Mauricio |
author_role |
author |
author2 |
Ribeiro,Ricardo Pereira Santos,Silvio Carlos Alves dos Goes,Elenice Souza dos Reis Souza,Felipe Pinheiro de Poveda-Parra,Angela Rocio Vargas,Lauro Urrea-Rojas,Angela Maria Lopera-Barrero,Nelson Mauricio |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Castro,Pedro Luiz de Ribeiro,Ricardo Pereira Santos,Silvio Carlos Alves dos Goes,Elenice Souza dos Reis Souza,Felipe Pinheiro de Poveda-Parra,Angela Rocio Vargas,Lauro Urrea-Rojas,Angela Maria Lopera-Barrero,Nelson Mauricio |
dc.subject.por.fl_str_mv |
Brycon orbignyanus conservation molecular markers heterologous primers |
topic |
Brycon orbignyanus conservation molecular markers heterologous primers |
description |
ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20170374 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.47 n.12 2017 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140552089075712 |