Estudo da viabilidade de microrganismo probiótico (Bifidobacterium ssp lactis) aplicado em produto cárneo cozido

• Autor(a) principal: Schossler, Luiza Sawitzki
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Manancial - Repositório Digital da UFSM
• Texto Completo: http://repositorio.ufsm.br/handle/1/5671
• topic: Alimentos funcionais; Probióticos; Bifidobacterium lactis; Produto cárneo; Patê de presunto; Functional food; Probiotics; Bifidobacterium lactis; Meat products; Ham pâté; CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS

This project was developed to prepare a cooked meat product, Ham Pate, with probiotic properties from the inoculation of the Bifidobacterium lactis probiotic organism in a concentration capable of ensuring its viability in the product. Ham pâté was processed in two different treatments and a control. Control (C) without addition of probiotic microorganisms; treatment 1, (T1) with the addition of probiotic microorganisms, Bifidobacterium lactis, in a final concentration expected in the product of 106 UFC. g-1; and, treatment 2 (T1) with the addition of probiotic microorganisms, Bifidobacterium lactis, in a final concentration expected in the product of 108 UFC. g-1. Preliminary tests of culture sensitivity to the sodium chloride (NaCl) and salt of curing (NaNO2) were performed. The concentrations of (NaCl) added to MRS agar were 1.0%, 1.5% and 2.0%, and (NaNO2) was added in 150, 200 and 250ppm. Tests with the combination of NaCl and (NaNO2) were also performed to verify the front line to the combination of these two salts, using the same concentrations, but combined. It was observed a growth of Bifidobacterium in all tests, that showed its resistance and suitability for using it in processed meat products that have these ingredients in their formulations. The developed product was tested by physical-chemical analysis (proximate composition, pH and TBARS), microbiological (coliform at 45º C, Clostridium perfringes, Staphylococcus coagulase positive, Salmonella sp. and Bifidobacterium lactis) and sensory. It was observed through the result of proximate composition analysis and pH that the product met all the requirements established by legislation. The determination of lipid oxidation by determination of thiobarbituric acid reactive substances (TBARS) showed that the control treatment resulted in lipid oxidation evolution much higher than other treatments (T1 and T2), suggesting that the probiotic microorganisms had an influence on stability of the product on the oxidation of lipids. The number of Bifidobacterium lactis viable cells showed in T1 initial concentrations of 5.6 x 106UFC. g-1, in the 14th day of the analysis fell into a cycle log. reaching in the 31st day a counting of viable cells of 4.3 x 105UFC.g-1, the T2 had initial concentration of 8.4 x 108UFC.g-1, suffering a dropping of 1 log cycle in the 31st day, ending with a concentration of 1.5 x 107 CFU. g-1. So, it s possible to conclude that treatment T1 was viable as probiotic food (counting at least 106UFC.g-1), for 14 days, while the treatment T2 was viable as probiotic during the entire period of analysis, in other words, for 31 days. The sensory analysis showed that there was no significant difference (p <0.05) for overall acceptance of treatments T1 and T2, both having a good acceptance on the test of the product purchase intention. Thus, it s possible to observe that there is a feasibility of probiotic microorganisms application in cooked meat products, such as ham pâté, it has more acceptance and it can be served alternative to the development of meat products with functional properties.