Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/4364 |
Resumo: | The traditional use of antibiotics in aquaculture as growth promoters has been limited due to the negative effects caused by these drugs. As an alternative to the use of these drugs has been sought manipulation of the microbiota of the gastrointestinal tract of aquatic animals through the use of oligosaccharides and dietary fibers with prebiotic potential. Thus, this study aimed to apply different methodologies to obtain Dietary Fiber Concentrates (DFC) = mucilage (MG); pectin (PN) and β-glucan + manan (βG + M) and evaluate the prebiotic potential of these supplements in the diet of juvenile jundiá (Rhamdia quelen). The determination of the nutritional composition of the ingredients revealed that the predominant component in all DFCs were dietary fiber and insoluble fiber. The DFC that had higher extraction yield was βG + M (19.81 ± 8.54%), followed by pectin (14.54% ± 2.72), and mucilage (7.18 ± 1.54%). The composition of mucilage and pectin had a greater diversity of monosaccharides, since the βG+M consisted primarily of mannose (74.5%) and glucose (24.3%). The supplementation of DFC in jundiás diet was assessed for eight weeks through study of growth, body nutrient deposition, digestive enzymes, biochemical and metabolic parameters, responses to stress and immune and intestinal morphology. The jundiás supplemented with DFCs achieved higher growth than the control group and similar to animals supplemented with 5 g kg-1 commercial prebiotic (CP 5). Most somatic parameters and whole fish proximate composition were influenced by supplementation of DFCs. The supplementation of pectin promoted lower activity of digestive enzymes in relation the control group. The animals supplemented with DFC obtained positive changes in biochemical parameters. Furthermore, jundiás showed no response to application of the stressor, maintaining basal cortisol levels. The fish supplemented with DFCs had higher hepatic glycogen stores in relation the control group. Moreover, supplementation with DFCs increased the height of intestinal villi of jundiá. However, these values were lower for the animals of the group PC 5. For thickness of the epithelium this variable was higher in the control group compared to animals supplemented with β-glucan+Manana. |
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Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen)Potential prebiotic of different dietary fiber concentrates in feed of juveniles jundiá (Rhamdia quelen)Promotor de crescimentoMucilagemPectinaβ-glicana+mananaPeixesGrowth promoterMucilagePectinβ-glucan+mannanFishCNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThe traditional use of antibiotics in aquaculture as growth promoters has been limited due to the negative effects caused by these drugs. As an alternative to the use of these drugs has been sought manipulation of the microbiota of the gastrointestinal tract of aquatic animals through the use of oligosaccharides and dietary fibers with prebiotic potential. Thus, this study aimed to apply different methodologies to obtain Dietary Fiber Concentrates (DFC) = mucilage (MG); pectin (PN) and β-glucan + manan (βG + M) and evaluate the prebiotic potential of these supplements in the diet of juvenile jundiá (Rhamdia quelen). The determination of the nutritional composition of the ingredients revealed that the predominant component in all DFCs were dietary fiber and insoluble fiber. The DFC that had higher extraction yield was βG + M (19.81 ± 8.54%), followed by pectin (14.54% ± 2.72), and mucilage (7.18 ± 1.54%). The composition of mucilage and pectin had a greater diversity of monosaccharides, since the βG+M consisted primarily of mannose (74.5%) and glucose (24.3%). The supplementation of DFC in jundiás diet was assessed for eight weeks through study of growth, body nutrient deposition, digestive enzymes, biochemical and metabolic parameters, responses to stress and immune and intestinal morphology. The jundiás supplemented with DFCs achieved higher growth than the control group and similar to animals supplemented with 5 g kg-1 commercial prebiotic (CP 5). Most somatic parameters and whole fish proximate composition were influenced by supplementation of DFCs. The supplementation of pectin promoted lower activity of digestive enzymes in relation the control group. The animals supplemented with DFC obtained positive changes in biochemical parameters. Furthermore, jundiás showed no response to application of the stressor, maintaining basal cortisol levels. The fish supplemented with DFCs had higher hepatic glycogen stores in relation the control group. Moreover, supplementation with DFCs increased the height of intestinal villi of jundiá. However, these values were lower for the animals of the group PC 5. For thickness of the epithelium this variable was higher in the control group compared to animals supplemented with β-glucan+Manana.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO uso tradicional de antibióticos na aquicultura como promotores de crescimento tem sido limitado em função dos efeitos negativos promovidos por estes medicamentos. Como alternativa ao uso destas drogas, tem se buscado a manipulação da microbiota do trato gastrointestinal dos animais aquáticos através da utilização de oligossacarídeos e de fibras alimentares com potencial prebiótico. Neste sentido, este estudo teve como objetivo aplicar metodologias para obtenção de diferentes Concentrados de Fibras Alimentares (CFAs) = Mucilagem (MG); Pectina (PN) e β-Glicana+Mananas (βG+M) e avaliar o potencial prebiótico destes suplementos na dieta de juvenis de jundiá (Rhamdia quelen). A determinação da composição nutricional dos ingredientes revelou que os componentes predominantes em todos os CFAs obtidos foram fibra alimentar total e fibra insolúvel. O CFA que apresentou maior rentabilidade de extração foi a βG+M (19,81%±8,54), seguida da Pectina (14,54%±2,72) e Mucilagem (7,18%±1,54). A composição da Mucilagem e Pectina obtiveram maior diversidade de monossacarídeos, já a βG+M consistiu basicamente de manose (74,5%) e glicose (24,3%). A suplementação dos CFAs na dieta de jundiás foi avaliada durante oito semanas, através de estudo de crescimento, deposição corporal de nutrientes, enzimas digestivas, parâmetros bioquímicos e metabólicos, resposta ao estresse e imunológica e morfometria intestinal. Os jundiás suplementados com os CFAs obtiveram crescimento superior em relação ao grupo controle e similar aos animais suplementados com 5 g kg-1 de prebiótico comercial (PC 5). A maioria dos parâmetros somáticos e de composição centesimal de peixe inteiro foram influenciados pela suplementação dos CFAs. A suplementação de Pectina promoveu menor atividade das enzimas digestivas em relação ao grupo controle. Os animais suplementados com os CFAs obtiveram alterações positivas nos parâmetros bioquímicos avaliados. Além disso, os jundiás não mostraram resposta à aplicação do agente estressor, mantendo os níveis de cortisol basal. Os peixes suplementados com os CFAs obtiveram maiores estoques de glicogênio hepático em relação ao grupo controle. Além do mais, a suplementação com os CFAs promoveu aumento na altura de vilos intestinais dos jundiás. Porém, estes valores foram menores em relação aos animais do grupo PC 5. Para espessura do epitélio (EE) esta variável foi maior no grupo Controle comparado aos animais suplementados com β- glicana + Manana.Universidade Federal de Santa MariaBRZootecniaUFSMPrograma de Pós-Graduação em ZootecniaSilva, Leila Picolli dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798001T6Fabregat, Thiago El Hadi Perezhttp://lattes.cnpq.br/1471046617364235Feiden, Aldihttp://lattes.cnpq.br/8384358462664823Viegas, Juliohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782452P7Radünz Neto, Joaohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783033E5Goulart, Fernanda Rodrigues2017-07-032017-07-032015-02-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfGOULART, Fernanda Rodrigues. Potential prebiotic of different dietary fiber concentrates in feed of juveniles jundiá (Rhamdia quelen). 2015. 149 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/4364porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-08-03T02:41:51Zoai:repositorio.ufsm.br:1/4364Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-08-03T02:41:51Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) Potential prebiotic of different dietary fiber concentrates in feed of juveniles jundiá (Rhamdia quelen) |
title |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) |
spellingShingle |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) Goulart, Fernanda Rodrigues Promotor de crescimento Mucilagem Pectina β-glicana+manana Peixes Growth promoter Mucilage Pectin β-glucan+mannan Fish CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
title_short |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) |
title_full |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) |
title_fullStr |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) |
title_full_unstemmed |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) |
title_sort |
Potencial prebiótico de diferentes concentrados de fibra alimentar na dieta de juvenis de jundiá (Rhamdia quelen) |
author |
Goulart, Fernanda Rodrigues |
author_facet |
Goulart, Fernanda Rodrigues |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Leila Picolli da http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798001T6 Fabregat, Thiago El Hadi Perez http://lattes.cnpq.br/1471046617364235 Feiden, Aldi http://lattes.cnpq.br/8384358462664823 Viegas, Julio http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782452P7 Radünz Neto, Joao http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783033E5 |
dc.contributor.author.fl_str_mv |
Goulart, Fernanda Rodrigues |
dc.subject.por.fl_str_mv |
Promotor de crescimento Mucilagem Pectina β-glicana+manana Peixes Growth promoter Mucilage Pectin β-glucan+mannan Fish CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
topic |
Promotor de crescimento Mucilagem Pectina β-glicana+manana Peixes Growth promoter Mucilage Pectin β-glucan+mannan Fish CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
description |
The traditional use of antibiotics in aquaculture as growth promoters has been limited due to the negative effects caused by these drugs. As an alternative to the use of these drugs has been sought manipulation of the microbiota of the gastrointestinal tract of aquatic animals through the use of oligosaccharides and dietary fibers with prebiotic potential. Thus, this study aimed to apply different methodologies to obtain Dietary Fiber Concentrates (DFC) = mucilage (MG); pectin (PN) and β-glucan + manan (βG + M) and evaluate the prebiotic potential of these supplements in the diet of juvenile jundiá (Rhamdia quelen). The determination of the nutritional composition of the ingredients revealed that the predominant component in all DFCs were dietary fiber and insoluble fiber. The DFC that had higher extraction yield was βG + M (19.81 ± 8.54%), followed by pectin (14.54% ± 2.72), and mucilage (7.18 ± 1.54%). The composition of mucilage and pectin had a greater diversity of monosaccharides, since the βG+M consisted primarily of mannose (74.5%) and glucose (24.3%). The supplementation of DFC in jundiás diet was assessed for eight weeks through study of growth, body nutrient deposition, digestive enzymes, biochemical and metabolic parameters, responses to stress and immune and intestinal morphology. The jundiás supplemented with DFCs achieved higher growth than the control group and similar to animals supplemented with 5 g kg-1 commercial prebiotic (CP 5). Most somatic parameters and whole fish proximate composition were influenced by supplementation of DFCs. The supplementation of pectin promoted lower activity of digestive enzymes in relation the control group. The animals supplemented with DFC obtained positive changes in biochemical parameters. Furthermore, jundiás showed no response to application of the stressor, maintaining basal cortisol levels. The fish supplemented with DFCs had higher hepatic glycogen stores in relation the control group. Moreover, supplementation with DFCs increased the height of intestinal villi of jundiá. However, these values were lower for the animals of the group PC 5. For thickness of the epithelium this variable was higher in the control group compared to animals supplemented with β-glucan+Manana. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-06 2017-07-03 2017-07-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GOULART, Fernanda Rodrigues. Potential prebiotic of different dietary fiber concentrates in feed of juveniles jundiá (Rhamdia quelen). 2015. 149 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2015. http://repositorio.ufsm.br/handle/1/4364 |
identifier_str_mv |
GOULART, Fernanda Rodrigues. Potential prebiotic of different dietary fiber concentrates in feed of juveniles jundiá (Rhamdia quelen). 2015. 149 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/4364 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Zootecnia UFSM Programa de Pós-Graduação em Zootecnia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Zootecnia UFSM Programa de Pós-Graduação em Zootecnia |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922159907831808 |