Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/0013000007p5s |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5731 |
Resumo: | This study aimed to verify the antioxidant activity of sun mushroom (Agaricus blazei Murrill) and its antioxidant effect in pork sausage. First drawn up the hydroethanolic extracts with different extraction times and temperatures, making the characterization of the total phenolics composition and antioxidant activity in vitro. After the extracts obtained at 70 ° C for 60 minutes at concentrations of 0%, 0.5%, 1.0% and 2.0% and also the sun mushroom powder, at concentrations of 0% , 1.0%, 2.0% and 4.0% were applied to pork sausage. Moisture, protein, ash and fat was analysed to characterize the products and every seven days was performed the analyzes of pH, color, TBARS values and microbiological counts. Sensory analysis was assessed by testing the acceptability through a hedonic scale with seven points and the purchase intent test. The results on the chemical composition, as well as the microbiological analysis for coagulase positive Staphylococcus, Coliforms at 35 º C and 45 º C, Salmonella and Clostridium sp sulfite reducer were in compliance with Brazilian legislation. With respect to product color, those treatments added sun mushrooms, both in extract form as powder, exhibited a yellowish color. At the end of the storage (21 days), the amount of TBARS for the sausages added with 2.0% of the sun mushroom extract was 0.705 ± 0.01 mg MDA/kg of sample and control 1.097 ± 0.11 mg MDA/kg of sample, while the 35th day, the amount of TBARS for sausages added with 1.0%, 2.0% and 4.0% sun mushroom powder was 0.604 ± 0.12 , 0.585 ± 0.11, 0.509 ± 0.12 mg MDA/Kg of sample, respectively, and control 1.131 ± 0.12 mg MDA/Kg of sample. The sun mushroom has no effect on the microbiological stability during storage, as assessed by counting the total mesophilic aerobic and psychrotrophic microorganisms. Regarding sensory characteristics showed good acceptability by consumers. Thus, it is concluded that the hydroethanolic extract of sun mushroom (Agaricus blazei Murrill) proved to be effective on the oxidative stability of pork sausage when added in a concentration of 2.0% by extending the shelf life up to 21 days storage, while in powder form was shown to be effective when added at a concentration of 1.0%, 2.0% and 4.0%, extending the shelf life up to 35 days under conditions of packaging permeable to oxygen and temperature cooling (+ 4 ° C) with a viable applicability as a natural antioxidant source with better performance in powder form. |
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Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiçaEvaluation of antioxidant activity of sun mushroom (Agaricus blazei Murril) and its application in fresh pork sausageAntioxidante naturalLinguica de carne suínaCogumelo do solOxidaçãoNatural antioxidantPork sausageSun mushroomOxidationCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSThis study aimed to verify the antioxidant activity of sun mushroom (Agaricus blazei Murrill) and its antioxidant effect in pork sausage. First drawn up the hydroethanolic extracts with different extraction times and temperatures, making the characterization of the total phenolics composition and antioxidant activity in vitro. After the extracts obtained at 70 ° C for 60 minutes at concentrations of 0%, 0.5%, 1.0% and 2.0% and also the sun mushroom powder, at concentrations of 0% , 1.0%, 2.0% and 4.0% were applied to pork sausage. Moisture, protein, ash and fat was analysed to characterize the products and every seven days was performed the analyzes of pH, color, TBARS values and microbiological counts. Sensory analysis was assessed by testing the acceptability through a hedonic scale with seven points and the purchase intent test. The results on the chemical composition, as well as the microbiological analysis for coagulase positive Staphylococcus, Coliforms at 35 º C and 45 º C, Salmonella and Clostridium sp sulfite reducer were in compliance with Brazilian legislation. With respect to product color, those treatments added sun mushrooms, both in extract form as powder, exhibited a yellowish color. At the end of the storage (21 days), the amount of TBARS for the sausages added with 2.0% of the sun mushroom extract was 0.705 ± 0.01 mg MDA/kg of sample and control 1.097 ± 0.11 mg MDA/kg of sample, while the 35th day, the amount of TBARS for sausages added with 1.0%, 2.0% and 4.0% sun mushroom powder was 0.604 ± 0.12 , 0.585 ± 0.11, 0.509 ± 0.12 mg MDA/Kg of sample, respectively, and control 1.131 ± 0.12 mg MDA/Kg of sample. The sun mushroom has no effect on the microbiological stability during storage, as assessed by counting the total mesophilic aerobic and psychrotrophic microorganisms. Regarding sensory characteristics showed good acceptability by consumers. Thus, it is concluded that the hydroethanolic extract of sun mushroom (Agaricus blazei Murrill) proved to be effective on the oxidative stability of pork sausage when added in a concentration of 2.0% by extending the shelf life up to 21 days storage, while in powder form was shown to be effective when added at a concentration of 1.0%, 2.0% and 4.0%, extending the shelf life up to 35 days under conditions of packaging permeable to oxygen and temperature cooling (+ 4 ° C) with a viable applicability as a natural antioxidant source with better performance in powder form.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO presente estudo teve por objetivo verificar a atividade antioxidante do cogumelo do sol (Agaricus blazei Murril) e seu efeito antioxidante em linguiça de carne suína. Primeiramente elaboraram-se os extratos hidroetanólicos com diferentes temperaturas e tempos de extração, realizando a caracterização da composição de fenólicos totais e a atividade antioxidante in vitro. Após, os extratos obtidos em temperatura de 70º C durante 60 minutos de extração, nas concentrações de 0 %, 0,5%, 1,0% e 2,0% e também o cogumelo do sol em pó, nas concentrações de 0 %, 1,0%, 2,0% e 4,0%, foram aplicados em linguiça de carne suína. As analises realizadas nos embutidos foram: composição centesimal (umidade, proteínas, cinzas, gordura) para a caracterização dos produtos e a cada sete dias realizadas as análises de pH, cor, índice de TBARS e análises microbiológicas. A análise sensorial foi avaliada através do teste de aceitabilidade com escala hedônica de sete pontos e teste de intenção de compra. Os resultados obtidos na composição centesimal, bem como nas análises microbiológicas para Staphylococcus coagulase positiva, Coliformes a 35 º C e a 45 º C, Samonella sp e Clostridium sulfito redutor dos produtos estavam de acordo com o exigido pela legislação brasileira. Com relação à cor do produto, todos aqueles tratamentos adicionados de cogumelo do sol, tanto na forma de extrato como em pó, apresentaram uma coloração com tonalidade amarelada. No período final de estocagem, ao 21º dia, o valor de TBARS para as linguiças adicionadas com 2,0% de extrato de cogumelo do sol foi de 0,705±0,01 mg MDA/kg de amostra e para o controle foi de 1,097±0,11 mg MDA/kg de amostra, enquanto que, ao 35º dia, o valor de TBARS para as linguiças adicionadas com 1,0%, 2,0% e 4,0% de cogumelo do sol em pó foi de 0,604±0,12; 0,585±0,11; 0,509±0,12 mg MDA/kg de amostra, respectivamente, e para o controle de 1,131±0,12 mg MDA/kg de amostra. O cogumelo do sol não possui efeito sobre a estabilidade microbiológica ao longo do armazenamento, avaliada pela contagem de microrganismos aeróbios mesófilos totais e psicrotróficos. Quanto às características sensoriais demonstrou boa aceitabilidade pelo consumidor. Desta forma, conclui-se que o extrato hidroetanólico de cogumelo do sol (Agaricus blazei Murril) mostrou ser efetivo sobre a estabilidade oxidativa de linguiça de carne suína quando adicionado na concentração de 2,0%, estendendo a vida de prateleira em até 21 dias de armazenamento, enquanto que na forma de pó mostrou ser efetivo quando adicionado na concentração de 1,0%, 2,0% e 4,0%, estendendo a vida de prateleira até 35 dias, nas condições de embalagem permeável ao oxigênio e temperatura de refrigeração (+ 4ºC). Logo, a aplicação de cogumelo do sol em linguiça de carne suína mostrou-se como uma fonte antioxidante natural viável com melhor desempenho na forma em pó.Universidade Federal de Santa MariaBRCiência e Tecnologia dos AlimentosUFSMPrograma de Pós-Graduação em Ciência e Tecnologia dos AlimentosKubota, Ernesto Hashimehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788191J1Sawitzki, Maristela Cortezhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708067H6Fries, Leadir Lucy Martinshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781614A3Stefanello, Flávia Santi2013-08-082013-08-082013-01-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSTEFANELLO, Flávia Santi. EVALUATION OF ANTIOXIDANT ACTIVITY OF SUN MUSHROOM (Agaricus blazei Murril) AND ITS APPLICATION IN FRESH PORK SAUSAGE. 2013. 94 f. Dissertação (Mestrado em Ciência e Tecnologia dos Alimentos) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/5731ark:/26339/0013000007p5sporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-02-18T17:46:51Zoai:repositorio.ufsm.br:1/5731Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-02-18T17:46:51Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça Evaluation of antioxidant activity of sun mushroom (Agaricus blazei Murril) and its application in fresh pork sausage |
title |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça |
spellingShingle |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça Stefanello, Flávia Santi Antioxidante natural Linguica de carne suína Cogumelo do sol Oxidação Natural antioxidant Pork sausage Sun mushroom Oxidation CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
title_short |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça |
title_full |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça |
title_fullStr |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça |
title_full_unstemmed |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça |
title_sort |
Avaliação da atividade antioxidante de cogumelo do sol (Agaricus blazei Murril) e sua aplicação em linguiça |
author |
Stefanello, Flávia Santi |
author_facet |
Stefanello, Flávia Santi |
author_role |
author |
dc.contributor.none.fl_str_mv |
Kubota, Ernesto Hashime http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788191J1 Sawitzki, Maristela Cortez http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708067H6 Fries, Leadir Lucy Martins http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781614A3 |
dc.contributor.author.fl_str_mv |
Stefanello, Flávia Santi |
dc.subject.por.fl_str_mv |
Antioxidante natural Linguica de carne suína Cogumelo do sol Oxidação Natural antioxidant Pork sausage Sun mushroom Oxidation CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
topic |
Antioxidante natural Linguica de carne suína Cogumelo do sol Oxidação Natural antioxidant Pork sausage Sun mushroom Oxidation CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
description |
This study aimed to verify the antioxidant activity of sun mushroom (Agaricus blazei Murrill) and its antioxidant effect in pork sausage. First drawn up the hydroethanolic extracts with different extraction times and temperatures, making the characterization of the total phenolics composition and antioxidant activity in vitro. After the extracts obtained at 70 ° C for 60 minutes at concentrations of 0%, 0.5%, 1.0% and 2.0% and also the sun mushroom powder, at concentrations of 0% , 1.0%, 2.0% and 4.0% were applied to pork sausage. Moisture, protein, ash and fat was analysed to characterize the products and every seven days was performed the analyzes of pH, color, TBARS values and microbiological counts. Sensory analysis was assessed by testing the acceptability through a hedonic scale with seven points and the purchase intent test. The results on the chemical composition, as well as the microbiological analysis for coagulase positive Staphylococcus, Coliforms at 35 º C and 45 º C, Salmonella and Clostridium sp sulfite reducer were in compliance with Brazilian legislation. With respect to product color, those treatments added sun mushrooms, both in extract form as powder, exhibited a yellowish color. At the end of the storage (21 days), the amount of TBARS for the sausages added with 2.0% of the sun mushroom extract was 0.705 ± 0.01 mg MDA/kg of sample and control 1.097 ± 0.11 mg MDA/kg of sample, while the 35th day, the amount of TBARS for sausages added with 1.0%, 2.0% and 4.0% sun mushroom powder was 0.604 ± 0.12 , 0.585 ± 0.11, 0.509 ± 0.12 mg MDA/Kg of sample, respectively, and control 1.131 ± 0.12 mg MDA/Kg of sample. The sun mushroom has no effect on the microbiological stability during storage, as assessed by counting the total mesophilic aerobic and psychrotrophic microorganisms. Regarding sensory characteristics showed good acceptability by consumers. Thus, it is concluded that the hydroethanolic extract of sun mushroom (Agaricus blazei Murrill) proved to be effective on the oxidative stability of pork sausage when added in a concentration of 2.0% by extending the shelf life up to 21 days storage, while in powder form was shown to be effective when added at a concentration of 1.0%, 2.0% and 4.0%, extending the shelf life up to 35 days under conditions of packaging permeable to oxygen and temperature cooling (+ 4 ° C) with a viable applicability as a natural antioxidant source with better performance in powder form. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-08 2013-08-08 2013-01-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
STEFANELLO, Flávia Santi. EVALUATION OF ANTIOXIDANT ACTIVITY OF SUN MUSHROOM (Agaricus blazei Murril) AND ITS APPLICATION IN FRESH PORK SAUSAGE. 2013. 94 f. Dissertação (Mestrado em Ciência e Tecnologia dos Alimentos) - Universidade Federal de Santa Maria, Santa Maria, 2013. http://repositorio.ufsm.br/handle/1/5731 |
dc.identifier.dark.fl_str_mv |
ark:/26339/0013000007p5s |
identifier_str_mv |
STEFANELLO, Flávia Santi. EVALUATION OF ANTIOXIDANT ACTIVITY OF SUN MUSHROOM (Agaricus blazei Murril) AND ITS APPLICATION IN FRESH PORK SAUSAGE. 2013. 94 f. Dissertação (Mestrado em Ciência e Tecnologia dos Alimentos) - Universidade Federal de Santa Maria, Santa Maria, 2013. ark:/26339/0013000007p5s |
url |
http://repositorio.ufsm.br/handle/1/5731 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Ciência e Tecnologia dos Alimentos UFSM Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Ciência e Tecnologia dos Alimentos UFSM Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1815172299867815936 |