Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos

Detalhes bibliográficos
Autor(a) principal: Paim, Aline Ferreira
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/0013000001cvm
Texto Completo: http://repositorio.ufsm.br/handle/1/8683
Resumo: Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos are native species with economic, ecological and silvicultural. They have problems with their spread via seeds, because they are recalcitrant and lose viability within a few weeks after collection. In addition, there is little information about the propagation of these species. Therefore, the general objective of this study was to evaluate methodologies which may contribute to micropropagation of E. involucrata and H. chrysotrichus. For multiplication of E. involucrata were tested different sources (BAP, KIN, 2iP and TDZ) and concentrations (0, 16 and 32 μM) of cytokinins added to the nutrient medium MS/2. Also, we tested the effect of TDZ (16 or 32 μM) combined with GA3 (0; 10; 20 and 40 μM) added in medium MS/2, on in vitro multiplication of nodal segments. Even for E. involucrata, we examined the effect of NAA (0; 0,5 and 1 μM) combined with TDZ (0; 16 and 32 μM), added to the medium MS/2. Finally, for E. involucrata, we tested the effect of different concentrations of TDZ (0; 16 and 32 μM) added to the nutrient medium MS/2, on the multiplication of shoot apices of seedlings germinated in vitro. For H. chrysotrichus were tested different types of explants (shoot apical segments and epicotyl) and nutrient media (WPM, MS, WPM/2 and MS/2) in the presence and absence of activated charcoal as well as the effect of BAP (0; 2; 4; 8 and 16 μM) on in vitro multiplication of this species. For in vitro multiplication of nodal segments of E. involucrata the cytokinins tested are dispensable; cytokinins favor the growth of bacterial contamination, high levels of contamination reduce in vitro survival and establishment of nodal segments; the in vitro establishment for this species is impaired by the occurrence of leaf chlorosis and swelling of the explants. As for H. chrysotrichus, the shoot apical segments show the highest development in the in vitro survival and establishment than of epicotyl ; there is a high in vitro swelling of the explants in the absence of growth regulators; in the in vitro establishment in medium WPM/2 in the presence of 0,05 μM shoot apical segments are more efficient under low concentrations of BAP; there isgreater calli formation in epicotyl with increase the concentration of BAP, the opposite was true for the shoot apical segment.
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spelling Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) MattosContributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) MattosAuxinasCitocininasSegmentos nodaisSegmento apical caulinarEpicótiloMultiplicação in vitroAuxinsCytokininsNodal segmentsShoot apical segmentEpicotylIn vitro multiplicationCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALEugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos are native species with economic, ecological and silvicultural. They have problems with their spread via seeds, because they are recalcitrant and lose viability within a few weeks after collection. In addition, there is little information about the propagation of these species. Therefore, the general objective of this study was to evaluate methodologies which may contribute to micropropagation of E. involucrata and H. chrysotrichus. For multiplication of E. involucrata were tested different sources (BAP, KIN, 2iP and TDZ) and concentrations (0, 16 and 32 μM) of cytokinins added to the nutrient medium MS/2. Also, we tested the effect of TDZ (16 or 32 μM) combined with GA3 (0; 10; 20 and 40 μM) added in medium MS/2, on in vitro multiplication of nodal segments. Even for E. involucrata, we examined the effect of NAA (0; 0,5 and 1 μM) combined with TDZ (0; 16 and 32 μM), added to the medium MS/2. Finally, for E. involucrata, we tested the effect of different concentrations of TDZ (0; 16 and 32 μM) added to the nutrient medium MS/2, on the multiplication of shoot apices of seedlings germinated in vitro. For H. chrysotrichus were tested different types of explants (shoot apical segments and epicotyl) and nutrient media (WPM, MS, WPM/2 and MS/2) in the presence and absence of activated charcoal as well as the effect of BAP (0; 2; 4; 8 and 16 μM) on in vitro multiplication of this species. For in vitro multiplication of nodal segments of E. involucrata the cytokinins tested are dispensable; cytokinins favor the growth of bacterial contamination, high levels of contamination reduce in vitro survival and establishment of nodal segments; the in vitro establishment for this species is impaired by the occurrence of leaf chlorosis and swelling of the explants. As for H. chrysotrichus, the shoot apical segments show the highest development in the in vitro survival and establishment than of epicotyl ; there is a high in vitro swelling of the explants in the absence of growth regulators; in the in vitro establishment in medium WPM/2 in the presence of 0,05 μM shoot apical segments are more efficient under low concentrations of BAP; there isgreater calli formation in epicotyl with increase the concentration of BAP, the opposite was true for the shoot apical segment.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorEugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos são espécies florestais nativas com importância econômica, silvicultural e ecológica. Possuem problemas quanto a sua propagação via sementes, pois estas são recalcitrantes, perdendo a viabilidade em poucas semanas após a coleta. Além disso, existem poucas informações sobre a propagação vegetativa dessas espécies. Considerado o exposto, o objetivo geral do presente estudo foi avaliar metodologias que possam contribuir para a micropropagação in vitro de E. involucrata e de H. chrysotrichus. Para a multiplicação de E. involucrata foram testadas diferentes fontes (BAP, CIN, TDZ e 2iP) e concentrações (0; 16 e 32 μM) de citocininas adicionadas ao meio nutritivo MS/2. Também foi testado o efeito de TDZ (16 e 32 μM) combinado a GA3 (0; 10; 20 e 40 μM), acrescentados em meio MS/2, na multiplicação in vitro de segmentos nodais. Ainda para E. involucrata, foi avaliado o efeito de ANA (0; 0,5 e 1 μM) combinado a TDZ (0; 16 e 32 μM), acrescidos ao meio MS/2. Por fim, para esta espécie, testou-se o efeito de diferentes concentrações de TDZ (0; 16 e 32 μM) adicionadas ao meio MS/2, na multiplicação de segmentos apicais caulinares obtidos de plântulas germinadas in vitro. Para H. chrysotrichus, foram testados diferentes tipos de explantes (segmento apical caulinar e epicótilo) e meios nutritivos (WPM, MS, WPM/2 e MS/2), na presença e ausência de carvão ativado, bem como o efeito de BAP (0; 2; 4; 8 e 16 μM) na multiplicação in vitro dessa espécie. Para multiplicação in vitro de segmentos nodais de E. involucrata, as citocininas testadas são dispensáveis, uma vez que favorecem o crescimento de contaminação bacteriana. Elevados índices de contaminação acarretam reduzida sobrevivência e estabelecimento in vitro de segmentos nodais; o estabelecimento in vitro para essa espécie é comprometido pela ocorrência de clorose foliar e intumescimento dos explantes. Já para H. chrysotrichus, o segmento apical caulinar apresenta desenvolvimento superior ao do epicótilo na sobrevivência e no estabelecimento in vitro; há elevado intumescimento in vitro dos explantes na ausência de fitorreguladores. No estabelecimento in vitro em meio WPM/2 na presença de 0,05 μM de ANA, os segmentos apicais caulinares são mais eficientes sob concentrações reduzidas de BAP; há maior calogênese em epicótilos à medida que aumenta a concentração de BAP, ocorrendo o contrário para o segmento apical caulinar.Universidade Federal de Santa MariaBRRecursos Florestais e Engenharia FlorestalUFSMPrograma de Pós-Graduação em Engenharia FlorestalReiniger, Lia Rejane Silveirahttp://lattes.cnpq.br/5739294882585391Flores, Rejanehttp://lattes.cnpq.br/0923837756333241Araujo, Maristela Machadohttp://lattes.cnpq.br/8810094509548250Paim, Aline Ferreira2011-11-082011-11-082011-07-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfPAIM, Aline Ferreira. Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos. 2011. 81 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/8683ark:/26339/0013000001cvmporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-03-18T19:07:03Zoai:repositorio.ufsm.br:1/8683Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:18:25.986467Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos
title Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
spellingShingle Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
Paim, Aline Ferreira
Auxinas
Citocininas
Segmentos nodais
Segmento apical caulinar
Epicótilo
Multiplicação in vitro
Auxins
Cytokinins
Nodal segments
Shoot apical segment
Epicotyl
In vitro multiplication
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
title_short Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
title_full Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
title_fullStr Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
title_full_unstemmed Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
title_sort Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
author Paim, Aline Ferreira
author_facet Paim, Aline Ferreira
author_role author
dc.contributor.none.fl_str_mv Reiniger, Lia Rejane Silveira
http://lattes.cnpq.br/5739294882585391
Flores, Rejane
http://lattes.cnpq.br/0923837756333241
Araujo, Maristela Machado
http://lattes.cnpq.br/8810094509548250
dc.contributor.author.fl_str_mv Paim, Aline Ferreira
dc.subject.por.fl_str_mv Auxinas
Citocininas
Segmentos nodais
Segmento apical caulinar
Epicótilo
Multiplicação in vitro
Auxins
Cytokinins
Nodal segments
Shoot apical segment
Epicotyl
In vitro multiplication
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
topic Auxinas
Citocininas
Segmentos nodais
Segmento apical caulinar
Epicótilo
Multiplicação in vitro
Auxins
Cytokinins
Nodal segments
Shoot apical segment
Epicotyl
In vitro multiplication
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
description Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos are native species with economic, ecological and silvicultural. They have problems with their spread via seeds, because they are recalcitrant and lose viability within a few weeks after collection. In addition, there is little information about the propagation of these species. Therefore, the general objective of this study was to evaluate methodologies which may contribute to micropropagation of E. involucrata and H. chrysotrichus. For multiplication of E. involucrata were tested different sources (BAP, KIN, 2iP and TDZ) and concentrations (0, 16 and 32 μM) of cytokinins added to the nutrient medium MS/2. Also, we tested the effect of TDZ (16 or 32 μM) combined with GA3 (0; 10; 20 and 40 μM) added in medium MS/2, on in vitro multiplication of nodal segments. Even for E. involucrata, we examined the effect of NAA (0; 0,5 and 1 μM) combined with TDZ (0; 16 and 32 μM), added to the medium MS/2. Finally, for E. involucrata, we tested the effect of different concentrations of TDZ (0; 16 and 32 μM) added to the nutrient medium MS/2, on the multiplication of shoot apices of seedlings germinated in vitro. For H. chrysotrichus were tested different types of explants (shoot apical segments and epicotyl) and nutrient media (WPM, MS, WPM/2 and MS/2) in the presence and absence of activated charcoal as well as the effect of BAP (0; 2; 4; 8 and 16 μM) on in vitro multiplication of this species. For in vitro multiplication of nodal segments of E. involucrata the cytokinins tested are dispensable; cytokinins favor the growth of bacterial contamination, high levels of contamination reduce in vitro survival and establishment of nodal segments; the in vitro establishment for this species is impaired by the occurrence of leaf chlorosis and swelling of the explants. As for H. chrysotrichus, the shoot apical segments show the highest development in the in vitro survival and establishment than of epicotyl ; there is a high in vitro swelling of the explants in the absence of growth regulators; in the in vitro establishment in medium WPM/2 in the presence of 0,05 μM shoot apical segments are more efficient under low concentrations of BAP; there isgreater calli formation in epicotyl with increase the concentration of BAP, the opposite was true for the shoot apical segment.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-08
2011-11-08
2011-07-18
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv PAIM, Aline Ferreira. Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos. 2011. 81 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2011.
http://repositorio.ufsm.br/handle/1/8683
dc.identifier.dark.fl_str_mv ark:/26339/0013000001cvm
identifier_str_mv PAIM, Aline Ferreira. Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos. 2011. 81 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2011.
ark:/26339/0013000001cvm
url http://repositorio.ufsm.br/handle/1/8683
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language por
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dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Recursos Florestais e Engenharia Florestal
UFSM
Programa de Pós-Graduação em Engenharia Florestal
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Recursos Florestais e Engenharia Florestal
UFSM
Programa de Pós-Graduação em Engenharia Florestal
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
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collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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