Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/0013000001cvm |
Texto Completo: | http://repositorio.ufsm.br/handle/1/8683 |
Resumo: | Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos are native species with economic, ecological and silvicultural. They have problems with their spread via seeds, because they are recalcitrant and lose viability within a few weeks after collection. In addition, there is little information about the propagation of these species. Therefore, the general objective of this study was to evaluate methodologies which may contribute to micropropagation of E. involucrata and H. chrysotrichus. For multiplication of E. involucrata were tested different sources (BAP, KIN, 2iP and TDZ) and concentrations (0, 16 and 32 μM) of cytokinins added to the nutrient medium MS/2. Also, we tested the effect of TDZ (16 or 32 μM) combined with GA3 (0; 10; 20 and 40 μM) added in medium MS/2, on in vitro multiplication of nodal segments. Even for E. involucrata, we examined the effect of NAA (0; 0,5 and 1 μM) combined with TDZ (0; 16 and 32 μM), added to the medium MS/2. Finally, for E. involucrata, we tested the effect of different concentrations of TDZ (0; 16 and 32 μM) added to the nutrient medium MS/2, on the multiplication of shoot apices of seedlings germinated in vitro. For H. chrysotrichus were tested different types of explants (shoot apical segments and epicotyl) and nutrient media (WPM, MS, WPM/2 and MS/2) in the presence and absence of activated charcoal as well as the effect of BAP (0; 2; 4; 8 and 16 μM) on in vitro multiplication of this species. For in vitro multiplication of nodal segments of E. involucrata the cytokinins tested are dispensable; cytokinins favor the growth of bacterial contamination, high levels of contamination reduce in vitro survival and establishment of nodal segments; the in vitro establishment for this species is impaired by the occurrence of leaf chlorosis and swelling of the explants. As for H. chrysotrichus, the shoot apical segments show the highest development in the in vitro survival and establishment than of epicotyl ; there is a high in vitro swelling of the explants in the absence of growth regulators; in the in vitro establishment in medium WPM/2 in the presence of 0,05 μM shoot apical segments are more efficient under low concentrations of BAP; there isgreater calli formation in epicotyl with increase the concentration of BAP, the opposite was true for the shoot apical segment. |
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Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) MattosContributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) MattosAuxinasCitocininasSegmentos nodaisSegmento apical caulinarEpicótiloMultiplicação in vitroAuxinsCytokininsNodal segmentsShoot apical segmentEpicotylIn vitro multiplicationCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALEugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos are native species with economic, ecological and silvicultural. They have problems with their spread via seeds, because they are recalcitrant and lose viability within a few weeks after collection. In addition, there is little information about the propagation of these species. Therefore, the general objective of this study was to evaluate methodologies which may contribute to micropropagation of E. involucrata and H. chrysotrichus. For multiplication of E. involucrata were tested different sources (BAP, KIN, 2iP and TDZ) and concentrations (0, 16 and 32 μM) of cytokinins added to the nutrient medium MS/2. Also, we tested the effect of TDZ (16 or 32 μM) combined with GA3 (0; 10; 20 and 40 μM) added in medium MS/2, on in vitro multiplication of nodal segments. Even for E. involucrata, we examined the effect of NAA (0; 0,5 and 1 μM) combined with TDZ (0; 16 and 32 μM), added to the medium MS/2. Finally, for E. involucrata, we tested the effect of different concentrations of TDZ (0; 16 and 32 μM) added to the nutrient medium MS/2, on the multiplication of shoot apices of seedlings germinated in vitro. For H. chrysotrichus were tested different types of explants (shoot apical segments and epicotyl) and nutrient media (WPM, MS, WPM/2 and MS/2) in the presence and absence of activated charcoal as well as the effect of BAP (0; 2; 4; 8 and 16 μM) on in vitro multiplication of this species. For in vitro multiplication of nodal segments of E. involucrata the cytokinins tested are dispensable; cytokinins favor the growth of bacterial contamination, high levels of contamination reduce in vitro survival and establishment of nodal segments; the in vitro establishment for this species is impaired by the occurrence of leaf chlorosis and swelling of the explants. As for H. chrysotrichus, the shoot apical segments show the highest development in the in vitro survival and establishment than of epicotyl ; there is a high in vitro swelling of the explants in the absence of growth regulators; in the in vitro establishment in medium WPM/2 in the presence of 0,05 μM shoot apical segments are more efficient under low concentrations of BAP; there isgreater calli formation in epicotyl with increase the concentration of BAP, the opposite was true for the shoot apical segment.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorEugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos são espécies florestais nativas com importância econômica, silvicultural e ecológica. Possuem problemas quanto a sua propagação via sementes, pois estas são recalcitrantes, perdendo a viabilidade em poucas semanas após a coleta. Além disso, existem poucas informações sobre a propagação vegetativa dessas espécies. Considerado o exposto, o objetivo geral do presente estudo foi avaliar metodologias que possam contribuir para a micropropagação in vitro de E. involucrata e de H. chrysotrichus. Para a multiplicação de E. involucrata foram testadas diferentes fontes (BAP, CIN, TDZ e 2iP) e concentrações (0; 16 e 32 μM) de citocininas adicionadas ao meio nutritivo MS/2. Também foi testado o efeito de TDZ (16 e 32 μM) combinado a GA3 (0; 10; 20 e 40 μM), acrescentados em meio MS/2, na multiplicação in vitro de segmentos nodais. Ainda para E. involucrata, foi avaliado o efeito de ANA (0; 0,5 e 1 μM) combinado a TDZ (0; 16 e 32 μM), acrescidos ao meio MS/2. Por fim, para esta espécie, testou-se o efeito de diferentes concentrações de TDZ (0; 16 e 32 μM) adicionadas ao meio MS/2, na multiplicação de segmentos apicais caulinares obtidos de plântulas germinadas in vitro. Para H. chrysotrichus, foram testados diferentes tipos de explantes (segmento apical caulinar e epicótilo) e meios nutritivos (WPM, MS, WPM/2 e MS/2), na presença e ausência de carvão ativado, bem como o efeito de BAP (0; 2; 4; 8 e 16 μM) na multiplicação in vitro dessa espécie. Para multiplicação in vitro de segmentos nodais de E. involucrata, as citocininas testadas são dispensáveis, uma vez que favorecem o crescimento de contaminação bacteriana. Elevados índices de contaminação acarretam reduzida sobrevivência e estabelecimento in vitro de segmentos nodais; o estabelecimento in vitro para essa espécie é comprometido pela ocorrência de clorose foliar e intumescimento dos explantes. Já para H. chrysotrichus, o segmento apical caulinar apresenta desenvolvimento superior ao do epicótilo na sobrevivência e no estabelecimento in vitro; há elevado intumescimento in vitro dos explantes na ausência de fitorreguladores. No estabelecimento in vitro em meio WPM/2 na presença de 0,05 μM de ANA, os segmentos apicais caulinares são mais eficientes sob concentrações reduzidas de BAP; há maior calogênese em epicótilos à medida que aumenta a concentração de BAP, ocorrendo o contrário para o segmento apical caulinar.Universidade Federal de Santa MariaBRRecursos Florestais e Engenharia FlorestalUFSMPrograma de Pós-Graduação em Engenharia FlorestalReiniger, Lia Rejane Silveirahttp://lattes.cnpq.br/5739294882585391Flores, Rejanehttp://lattes.cnpq.br/0923837756333241Araujo, Maristela Machadohttp://lattes.cnpq.br/8810094509548250Paim, Aline Ferreira2011-11-082011-11-082011-07-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfPAIM, Aline Ferreira. Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos. 2011. 81 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/8683ark:/26339/0013000001cvmporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-03-18T19:07:03Zoai:repositorio.ufsm.br:1/8683Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:18:25.986467Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos |
title |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos |
spellingShingle |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos Paim, Aline Ferreira Auxinas Citocininas Segmentos nodais Segmento apical caulinar Epicótilo Multiplicação in vitro Auxins Cytokinins Nodal segments Shoot apical segment Epicotyl In vitro multiplication CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
title_short |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos |
title_full |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos |
title_fullStr |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos |
title_full_unstemmed |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos |
title_sort |
Contribuições para a micropropagação de Eugenia involucrata DC. e Handroanthus chrysotrichus (Mart. ex DC) Mattos |
author |
Paim, Aline Ferreira |
author_facet |
Paim, Aline Ferreira |
author_role |
author |
dc.contributor.none.fl_str_mv |
Reiniger, Lia Rejane Silveira http://lattes.cnpq.br/5739294882585391 Flores, Rejane http://lattes.cnpq.br/0923837756333241 Araujo, Maristela Machado http://lattes.cnpq.br/8810094509548250 |
dc.contributor.author.fl_str_mv |
Paim, Aline Ferreira |
dc.subject.por.fl_str_mv |
Auxinas Citocininas Segmentos nodais Segmento apical caulinar Epicótilo Multiplicação in vitro Auxins Cytokinins Nodal segments Shoot apical segment Epicotyl In vitro multiplication CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
topic |
Auxinas Citocininas Segmentos nodais Segmento apical caulinar Epicótilo Multiplicação in vitro Auxins Cytokinins Nodal segments Shoot apical segment Epicotyl In vitro multiplication CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
description |
Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos are native species with economic, ecological and silvicultural. They have problems with their spread via seeds, because they are recalcitrant and lose viability within a few weeks after collection. In addition, there is little information about the propagation of these species. Therefore, the general objective of this study was to evaluate methodologies which may contribute to micropropagation of E. involucrata and H. chrysotrichus. For multiplication of E. involucrata were tested different sources (BAP, KIN, 2iP and TDZ) and concentrations (0, 16 and 32 μM) of cytokinins added to the nutrient medium MS/2. Also, we tested the effect of TDZ (16 or 32 μM) combined with GA3 (0; 10; 20 and 40 μM) added in medium MS/2, on in vitro multiplication of nodal segments. Even for E. involucrata, we examined the effect of NAA (0; 0,5 and 1 μM) combined with TDZ (0; 16 and 32 μM), added to the medium MS/2. Finally, for E. involucrata, we tested the effect of different concentrations of TDZ (0; 16 and 32 μM) added to the nutrient medium MS/2, on the multiplication of shoot apices of seedlings germinated in vitro. For H. chrysotrichus were tested different types of explants (shoot apical segments and epicotyl) and nutrient media (WPM, MS, WPM/2 and MS/2) in the presence and absence of activated charcoal as well as the effect of BAP (0; 2; 4; 8 and 16 μM) on in vitro multiplication of this species. For in vitro multiplication of nodal segments of E. involucrata the cytokinins tested are dispensable; cytokinins favor the growth of bacterial contamination, high levels of contamination reduce in vitro survival and establishment of nodal segments; the in vitro establishment for this species is impaired by the occurrence of leaf chlorosis and swelling of the explants. As for H. chrysotrichus, the shoot apical segments show the highest development in the in vitro survival and establishment than of epicotyl ; there is a high in vitro swelling of the explants in the absence of growth regulators; in the in vitro establishment in medium WPM/2 in the presence of 0,05 μM shoot apical segments are more efficient under low concentrations of BAP; there isgreater calli formation in epicotyl with increase the concentration of BAP, the opposite was true for the shoot apical segment. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11-08 2011-11-08 2011-07-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PAIM, Aline Ferreira. Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos. 2011. 81 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/8683 |
dc.identifier.dark.fl_str_mv |
ark:/26339/0013000001cvm |
identifier_str_mv |
PAIM, Aline Ferreira. Contributions to micropropagation of Eugenia involucrata DC. and Handroanthus chrysotrichus (Mart. ex DC) Mattos. 2011. 81 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2011. ark:/26339/0013000001cvm |
url |
http://repositorio.ufsm.br/handle/1/8683 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Recursos Florestais e Engenharia Florestal UFSM Programa de Pós-Graduação em Engenharia Florestal |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Recursos Florestais e Engenharia Florestal UFSM Programa de Pós-Graduação em Engenharia Florestal |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1814439710016143360 |