Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/26510 http://dx.doi.org/10.1016/S0014-5793(01)02275-X |
Resumo: | Acidic glycosphingolipid components were extracted from the yeast form of the dimorphic mycopathogen Sporothrix schenckii. Two minor and the major fraction from the yeast form (Ss-Y1, -Y2, and -Y6. respectively) have been isolated. By a combination of 1- and 2-D H-1-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and gas chromatography/mass spectrometry (GC/MS). Ss-Y6 was determined to be triglycosylinositol phosphorylceramide with a novel glycan structure, Man alpha1 --> 3Man alpha1 --> 6GlcNH(2)alpha1 --> 2Ins1-P-1Cer (where Ins = myo-inositol, P = phosphodiester), While the GlcNH(2)alpha1 --> 6Ins1-P-motif is found widely distributed in eukaryotic GPI anchors, the linkage GlcNH(2)alpha1 --> 2Insl-P- has not been previously observed in any glycolipid, Ss-Y1 and Ss-Y2 were both found to have the known glycan structure Man alpha1 --> 3Man alpha1 --> 2Ins1-P-1Cer, Together with the results of a prior study [Toledo et al, (2001) Biochem, Biophys. Res. Commun, 280, 19-24] which showed that the mycelium form expresses GIPCs with the structures Man alpha1 --> 6Ins1-P-1Cer and Man alpha1 --> 3Man alpha1 --> 6Ins1-P-1Cer, these results demonstrate that S, schenckii can synthesize glycosylinositol phosphorylceramides with at least three different core Linkages, (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved. |
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Toledo, Marcos Sergio de [UNIFESP]Levery, S. B.Straus, Anita Hilda [UNIFESP]Takahashi, Helio Kiyoshi [UNIFESP]Univ GeorgiaUniversidade Federal de São Paulo (UNIFESP)2016-01-24T12:31:20Z2016-01-24T12:31:20Z2001-03-23Febs Letters. Amsterdam: Elsevier B.V., v. 493, n. 1, p. 50-56, 2001.0014-5793http://repositorio.unifesp.br/handle/11600/26510http://dx.doi.org/10.1016/S0014-5793(01)02275-XWOS000167822200011.pdf10.1016/S0014-5793(01)02275-XWOS:000167822200011Acidic glycosphingolipid components were extracted from the yeast form of the dimorphic mycopathogen Sporothrix schenckii. Two minor and the major fraction from the yeast form (Ss-Y1, -Y2, and -Y6. respectively) have been isolated. By a combination of 1- and 2-D H-1-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and gas chromatography/mass spectrometry (GC/MS). Ss-Y6 was determined to be triglycosylinositol phosphorylceramide with a novel glycan structure, Man alpha1 --> 3Man alpha1 --> 6GlcNH(2)alpha1 --> 2Ins1-P-1Cer (where Ins = myo-inositol, P = phosphodiester), While the GlcNH(2)alpha1 --> 6Ins1-P-motif is found widely distributed in eukaryotic GPI anchors, the linkage GlcNH(2)alpha1 --> 2Insl-P- has not been previously observed in any glycolipid, Ss-Y1 and Ss-Y2 were both found to have the known glycan structure Man alpha1 --> 3Man alpha1 --> 2Ins1-P-1Cer, Together with the results of a prior study [Toledo et al, (2001) Biochem, Biophys. Res. Commun, 280, 19-24] which showed that the mycelium form expresses GIPCs with the structures Man alpha1 --> 6Ins1-P-1Cer and Man alpha1 --> 3Man alpha1 --> 6Ins1-P-1Cer, these results demonstrate that S, schenckii can synthesize glycosylinositol phosphorylceramides with at least three different core Linkages, (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USAUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, BrazilUniv Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USAUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, BrazilWeb of Science50-56engElsevier B.V.Febs Lettershttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccesssphingolipidglycolipiddimorphismmycopathogennuclear magnetic resonancemass spectrometrySphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motifinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000167822200011.pdfapplication/pdf319741${dspace.ui.url}/bitstream/11600/26510/1/WOS000167822200011.pdf610338bfeef3668277c5105659587ad8MD51open accessTEXTWOS000167822200011.pdf.txtWOS000167822200011.pdf.txtExtracted texttext/plain25259${dspace.ui.url}/bitstream/11600/26510/2/WOS000167822200011.pdf.txt5c2a46c0f783a991115e45aad6d921e1MD52open access11600/265102022-11-04 14:22:12.181open accessoai:repositorio.unifesp.br:11600/26510Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-04T17:22:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif |
title |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif |
spellingShingle |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif Toledo, Marcos Sergio de [UNIFESP] sphingolipid glycolipid dimorphism mycopathogen nuclear magnetic resonance mass spectrometry |
title_short |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif |
title_full |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif |
title_fullStr |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif |
title_full_unstemmed |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif |
title_sort |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH(2)alpha 1 -> 2Ins motif |
author |
Toledo, Marcos Sergio de [UNIFESP] |
author_facet |
Toledo, Marcos Sergio de [UNIFESP] Levery, S. B. Straus, Anita Hilda [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] |
author_role |
author |
author2 |
Levery, S. B. Straus, Anita Hilda [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Georgia Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Toledo, Marcos Sergio de [UNIFESP] Levery, S. B. Straus, Anita Hilda [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] |
dc.subject.eng.fl_str_mv |
sphingolipid glycolipid dimorphism mycopathogen nuclear magnetic resonance mass spectrometry |
topic |
sphingolipid glycolipid dimorphism mycopathogen nuclear magnetic resonance mass spectrometry |
description |
Acidic glycosphingolipid components were extracted from the yeast form of the dimorphic mycopathogen Sporothrix schenckii. Two minor and the major fraction from the yeast form (Ss-Y1, -Y2, and -Y6. respectively) have been isolated. By a combination of 1- and 2-D H-1-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and gas chromatography/mass spectrometry (GC/MS). Ss-Y6 was determined to be triglycosylinositol phosphorylceramide with a novel glycan structure, Man alpha1 --> 3Man alpha1 --> 6GlcNH(2)alpha1 --> 2Ins1-P-1Cer (where Ins = myo-inositol, P = phosphodiester), While the GlcNH(2)alpha1 --> 6Ins1-P-motif is found widely distributed in eukaryotic GPI anchors, the linkage GlcNH(2)alpha1 --> 2Insl-P- has not been previously observed in any glycolipid, Ss-Y1 and Ss-Y2 were both found to have the known glycan structure Man alpha1 --> 3Man alpha1 --> 2Ins1-P-1Cer, Together with the results of a prior study [Toledo et al, (2001) Biochem, Biophys. Res. Commun, 280, 19-24] which showed that the mycelium form expresses GIPCs with the structures Man alpha1 --> 6Ins1-P-1Cer and Man alpha1 --> 3Man alpha1 --> 6Ins1-P-1Cer, these results demonstrate that S, schenckii can synthesize glycosylinositol phosphorylceramides with at least three different core Linkages, (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-03-23 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:31:20Z |
dc.date.available.fl_str_mv |
2016-01-24T12:31:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Febs Letters. Amsterdam: Elsevier B.V., v. 493, n. 1, p. 50-56, 2001. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/26510 http://dx.doi.org/10.1016/S0014-5793(01)02275-X |
dc.identifier.issn.none.fl_str_mv |
0014-5793 |
dc.identifier.file.none.fl_str_mv |
WOS000167822200011.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/S0014-5793(01)02275-X |
dc.identifier.wos.none.fl_str_mv |
WOS:000167822200011 |
identifier_str_mv |
Febs Letters. Amsterdam: Elsevier B.V., v. 493, n. 1, p. 50-56, 2001. 0014-5793 WOS000167822200011.pdf 10.1016/S0014-5793(01)02275-X WOS:000167822200011 |
url |
http://repositorio.unifesp.br/handle/11600/26510 http://dx.doi.org/10.1016/S0014-5793(01)02275-X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Febs Letters |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
dc.format.none.fl_str_mv |
50-56 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
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Elsevier B.V. |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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