A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/30456 https://dx.doi.org/10.1093/glycob/cwm139 |
Resumo: | The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1. |
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Farias, Eduardo Henrique Cunha de [UNIFESP]Pomin, Vitor HugoValente, Ana-PaulaNader, Helena Bociani [UNIFESP]Rocha, Hugo Alexandre de OliveiraMourao, Paulo Antonio de SouzaUniv Fed Rio Grande do NorteHosp Univ Clementino Fraga FilhoUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)2016-01-24T13:49:35Z2016-01-24T13:49:35Z2008-03-01The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1.Univ Fed Rio Grande do Norte, Dept Bioquim, BIOPOL, Lab Biotechnol Polimeros Nat, BR-59072970 Natal, RN, BrazilHosp Univ Clementino Fraga Filho, Lab Tecido Conjuntivo, Natal, RN, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Ctr Ciencias Saude, BR-21941590 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Ctr Nacl Ressonancia Magnet Nucl Jiri Jonas, BR-21941590 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilWeb of Science250-259Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008.10.1093/glycob/cwm1390959-6658https://repositorio.unifesp.br/handle/11600/30456https://dx.doi.org/10.1093/glycob/cwm139WOS:000253832400007engOxford Univ Press IncGlycobiologyhttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlinfo:eu-repo/semantics/openAccessCodium isthmocladumGreen algaGreen seaweedPhylogenyPyruvylated-sulfated galactanA preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/304562024-01-19 19:28:16.5oai:repositorio.unifesp.br/:11600/30456https://repositorio.unifesp.brRepositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652024-01-19T19:28:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum |
title |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum |
spellingShingle |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum Farias, Eduardo Henrique Cunha de [UNIFESP] Codium isthmocladum Green alga Green seaweed Phylogeny Pyruvylated-sulfated galactan |
title_short |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum |
title_full |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum |
title_fullStr |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum |
title_full_unstemmed |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum |
title_sort |
A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum |
author |
Farias, Eduardo Henrique Cunha de [UNIFESP] |
author_facet |
Farias, Eduardo Henrique Cunha de [UNIFESP] Pomin, Vitor Hugo Valente, Ana-Paula Nader, Helena Bociani [UNIFESP] Rocha, Hugo Alexandre de Oliveira Mourao, Paulo Antonio de Souza |
author_role |
author |
author2 |
Pomin, Vitor Hugo Valente, Ana-Paula Nader, Helena Bociani [UNIFESP] Rocha, Hugo Alexandre de Oliveira Mourao, Paulo Antonio de Souza |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Fed Rio Grande do Norte Hosp Univ Clementino Fraga Filho Universidade Federal do Rio de Janeiro (UFRJ) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Farias, Eduardo Henrique Cunha de [UNIFESP] Pomin, Vitor Hugo Valente, Ana-Paula Nader, Helena Bociani [UNIFESP] Rocha, Hugo Alexandre de Oliveira Mourao, Paulo Antonio de Souza |
dc.subject.eng.fl_str_mv |
Codium isthmocladum Green alga Green seaweed Phylogeny Pyruvylated-sulfated galactan |
topic |
Codium isthmocladum Green alga Green seaweed Phylogeny Pyruvylated-sulfated galactan |
description |
The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-03-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:49:35Z |
dc.date.available.fl_str_mv |
2016-01-24T13:49:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/30456 https://dx.doi.org/10.1093/glycob/cwm139 |
dc.identifier.doi.none.fl_str_mv |
10.1093/glycob/cwm139 |
dc.identifier.issn.none.fl_str_mv |
0959-6658 |
dc.identifier.wos.none.fl_str_mv |
WOS:000253832400007 |
identifier_str_mv |
Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008. 10.1093/glycob/cwm139 0959-6658 WOS:000253832400007 |
url |
https://repositorio.unifesp.br/handle/11600/30456 https://dx.doi.org/10.1093/glycob/cwm139 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Glycobiology |
dc.rights.driver.fl_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
250-259 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press Inc |
publisher.none.fl_str_mv |
Oxford Univ Press Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803210217382477824 |