A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum

Detalhes bibliográficos
Autor(a) principal: Farias, Eduardo H. C.
Data de Publicação: 2008
Outros Autores: Pomin, Vitor H., Valente, Ana-Paula, Nader, Helena Bociani [UNIFESP], Rocha, Hugo A. O., Mourao, Paulo A. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/30456
http://dx.doi.org/10.1093/glycob/cwm139
Resumo: The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1.
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spelling Farias, Eduardo H. C.Pomin, Vitor H.Valente, Ana-PaulaNader, Helena Bociani [UNIFESP]Rocha, Hugo A. O.Mourao, Paulo A. S.Univ Fed Rio Grande do NorteHosp Univ Clementino Fraga FilhoUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)2016-01-24T13:49:35Z2016-01-24T13:49:35Z2008-03-01Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008.0959-6658http://repositorio.unifesp.br/handle/11600/30456http://dx.doi.org/10.1093/glycob/cwm13910.1093/glycob/cwm139WOS:000253832400007The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1.Univ Fed Rio Grande do Norte, Dept Bioquim, BIOPOL, Lab Biotechnol Polimeros Nat, BR-59072970 Natal, RN, BrazilHosp Univ Clementino Fraga Filho, Lab Tecido Conjuntivo, Natal, RN, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med, Ctr Ciencias Saude, BR-21941590 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Ctr Nacl Ressonancia Magnet Nucl Jiri Jonas, BR-21941590 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilWeb of Science250-259engOxford Univ Press IncGlycobiologyhttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlinfo:eu-repo/semantics/openAccessCodium isthmocladumgreen algagreen seaweedphylogenypyruvylated-sulfated galactanA preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/304562022-09-27 09:38:18.814metadata only accessoai:repositorio.unifesp.br:11600/30456Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T12:38:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
spellingShingle A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
Farias, Eduardo H. C.
Codium isthmocladum
green alga
green seaweed
phylogeny
pyruvylated-sulfated galactan
title_short A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_full A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_fullStr A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_full_unstemmed A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
title_sort A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum
author Farias, Eduardo H. C.
author_facet Farias, Eduardo H. C.
Pomin, Vitor H.
Valente, Ana-Paula
Nader, Helena Bociani [UNIFESP]
Rocha, Hugo A. O.
Mourao, Paulo A. S.
author_role author
author2 Pomin, Vitor H.
Valente, Ana-Paula
Nader, Helena Bociani [UNIFESP]
Rocha, Hugo A. O.
Mourao, Paulo A. S.
author2_role author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Univ Fed Rio Grande do Norte
Hosp Univ Clementino Fraga Filho
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Farias, Eduardo H. C.
Pomin, Vitor H.
Valente, Ana-Paula
Nader, Helena Bociani [UNIFESP]
Rocha, Hugo A. O.
Mourao, Paulo A. S.
dc.subject.eng.fl_str_mv Codium isthmocladum
green alga
green seaweed
phylogeny
pyruvylated-sulfated galactan
topic Codium isthmocladum
green alga
green seaweed
phylogeny
pyruvylated-sulfated galactan
description The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, similar to 14 kDa, and SG 2, similar to 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H1D NMR spectra. the structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. in minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1' carboxy)ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1.
publishDate 2008
dc.date.issued.fl_str_mv 2008-03-01
dc.date.accessioned.fl_str_mv 2016-01-24T13:49:35Z
dc.date.available.fl_str_mv 2016-01-24T13:49:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/30456
http://dx.doi.org/10.1093/glycob/cwm139
dc.identifier.issn.none.fl_str_mv 0959-6658
dc.identifier.doi.none.fl_str_mv 10.1093/glycob/cwm139
dc.identifier.wos.none.fl_str_mv WOS:000253832400007
identifier_str_mv Glycobiology. Cary: Oxford Univ Press Inc, v. 18, n. 3, p. 250-259, 2008.
0959-6658
10.1093/glycob/cwm139
WOS:000253832400007
url http://repositorio.unifesp.br/handle/11600/30456
http://dx.doi.org/10.1093/glycob/cwm139
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Glycobiology
dc.rights.driver.fl_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 250-259
dc.publisher.none.fl_str_mv Oxford Univ Press Inc
publisher.none.fl_str_mv Oxford Univ Press Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
_version_ 1802764162566193152

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