Cutaneous changes in rats induced by chronic skin exposure to ultraviolet radiation and organophosphate pesticide

• Autor(a) principal: Fernandes, Tania Rita Moreno de Oliveira [UNIFESP]
• Data de Publicação: 2014
• Outros Autores: Santos, Itamar [UNIFESP], Korinsfky, Juliana Pedrosa, Silva, Benedyto Savio de Lima e [UNIFESP], Carvalho, Luis Otavio, Plapler, Helio [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-86502014000100002; http://repositorio.unifesp.br/handle/11600/41986
• topic: Epidermis; Solar Radiation; Skin Neoplasms; Organophosphates; Rats

PURPOSE: To study the possible potentiation of the carcinogenic effects of ultraviolet radiation associated with an organophosphate pesticide.METHODS: Forty Wistar rats were assigned into four groups (n=10 each) randomized according to the procedures: group A received only UVR-B radiation; group B, UVR-B for eight weeks followed by a seven week period of pesticide exposure; group C, UVR-B + pesticide concomitantly: group D, only pesticide application. At the end of the fifth, tenth and fifteenth weeks the animals were photographed. Skin biopsy and histopathological study with Hematoxylin-Eosin were done on the fifteenth week. Statistical analysis with Fisher's and Sign (unilateral) tests, 5% value for significance.RESULTS: Macroscopic lesions in the group A evolved from the erythema to erythema + desquamation. The groups B and C, with the association of two carcinogens, and group D presented evolution to keratosis, with higher incidence in group D. The histology showed a significant increase in the severity of injuries when the UVR-B and the pesticide were applied simultaneously, leading to cellular atypia.CONCLUSIONS: Concurrent association of UVR-B to organophosphate pesticide produced more severe lesions microscopically, although this has not been so apparent macroscopically. In daily practice the clinical evaluation should be complemented with laboratory evaluation.