Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33658 http://dx.doi.org/10.1371/journal.pntd.0001182 |
Resumo: | Background: Accidents caused by Loxosceles spider may cause severe systemic reactions, including acute kidney injury (AKI). There are few experimental studies assessing Loxosceles venom effects on kidney function in vivo.Methodology/Principal Findings: in order to test Loxosceles gaucho venom (LV) nephrotoxicity and to assess some of the possible mechanisms of renal injury, rats were studied up to 60 minutes after LV 0.24 mg/kg or saline IV injection (control). LV caused a sharp and significant drop in glomerular filtration rate, renal blood flow and urinary output and increased renal vascular resistance, without changing blood pressure. Venom infusion increased significantly serum creatine kinase and aspartate aminotransferase. in the LV group renal histology analysis found acute epithelial tubular cells degenerative changes, presence of cell debris and detached epithelial cells in tubular lumen without glomerular or vascular changes. Immunohistochemistry disclosed renal deposition of myoglobin and hemoglobin. LV did not cause injury to a suspension of fresh proximal tubules isolated from rats.Conclusions/Significance: Loxosceles gaucho venom injection caused early AKI, which occurred without blood pressure variation. Changes in glomerular function occurred likely due to renal vasoconstriction and rhabdomyolysis. Direct nephrotoxicity could not be demonstrated in vitro. the development of a consistent model of Loxosceles venom-induced AKI and a better understanding of the mechanisms involved in the renal injury may allow more efficient ways to prevent or attenuate the systemic injury after Loxosceles bite. |
id |
UFSP_0a023896d35606460c501d3a8d9733d0 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/33658 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Lucato Junior, Rui Vicente [UNIFESP]Abdulkader, Regina C. R. M.Barbaro, Katia C.Mendes, Gloria E.Castro, IsacBaptista, Maria A. S. F.Cury, Patricia M.Malheiros, Denise M. C.Schor, Nestor [UNIFESP]Yu, LuisBurdmann, Emmanuel A.Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Butantan Inst2016-01-24T14:16:40Z2016-01-24T14:16:40Z2011-05-01Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 5, n. 5, 5 p., 2011.1935-2727http://repositorio.unifesp.br/handle/11600/33658http://dx.doi.org/10.1371/journal.pntd.0001182WOS000291099100043.pdf10.1371/journal.pntd.0001182WOS:000291099100043Background: Accidents caused by Loxosceles spider may cause severe systemic reactions, including acute kidney injury (AKI). There are few experimental studies assessing Loxosceles venom effects on kidney function in vivo.Methodology/Principal Findings: in order to test Loxosceles gaucho venom (LV) nephrotoxicity and to assess some of the possible mechanisms of renal injury, rats were studied up to 60 minutes after LV 0.24 mg/kg or saline IV injection (control). LV caused a sharp and significant drop in glomerular filtration rate, renal blood flow and urinary output and increased renal vascular resistance, without changing blood pressure. Venom infusion increased significantly serum creatine kinase and aspartate aminotransferase. in the LV group renal histology analysis found acute epithelial tubular cells degenerative changes, presence of cell debris and detached epithelial cells in tubular lumen without glomerular or vascular changes. Immunohistochemistry disclosed renal deposition of myoglobin and hemoglobin. LV did not cause injury to a suspension of fresh proximal tubules isolated from rats.Conclusions/Significance: Loxosceles gaucho venom injection caused early AKI, which occurred without blood pressure variation. Changes in glomerular function occurred likely due to renal vasoconstriction and rhabdomyolysis. Direct nephrotoxicity could not be demonstrated in vitro. the development of a consistent model of Loxosceles venom-induced AKI and a better understanding of the mechanisms involved in the renal injury may allow more efficient ways to prevent or attenuate the systemic injury after Loxosceles bite.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Jose Rio Preto Med Sch, Div Nephrol, Sao Jose Do Rio Preto, BrazilUniversidade Federal de São Paulo, Div Nephrol, São Paulo, BrazilUniv São Paulo Med Sch, Div Nephrol, São Paulo, BrazilButantan Inst, Immunopathol Lab, São Paulo, BrazilSao Jose Rio Preto Med Sch, Dept Pathol, Sao Jose Do Rio Preto, BrazilUniversidade Federal de São Paulo, Div Nephrol, São Paulo, BrazilWeb of Science5engPublic Library SciencePlos Neglected Tropical DiseasesLoxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000291099100043.pdfapplication/pdf162276${dspace.ui.url}/bitstream/11600/33658/1/WOS000291099100043.pdf94f277d447dedaa78947f9ff4c33b626MD51open accessTEXTWOS000291099100043.pdf.txtWOS000291099100043.pdf.txtExtracted texttext/plain31054${dspace.ui.url}/bitstream/11600/33658/2/WOS000291099100043.pdf.txtad8287a1e5309cadba5b40da3b77007bMD52open access11600/336582022-11-03 10:40:51.478open accessoai:repositorio.unifesp.br:11600/33658Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-03T13:40:51Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies |
title |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies |
spellingShingle |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies Lucato Junior, Rui Vicente [UNIFESP] |
title_short |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies |
title_full |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies |
title_fullStr |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies |
title_full_unstemmed |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies |
title_sort |
Loxosceles gaucho Venom-Induced Acute Kidney Injury - in Vivo and in Vitro Studies |
author |
Lucato Junior, Rui Vicente [UNIFESP] |
author_facet |
Lucato Junior, Rui Vicente [UNIFESP] Abdulkader, Regina C. R. M. Barbaro, Katia C. Mendes, Gloria E. Castro, Isac Baptista, Maria A. S. F. Cury, Patricia M. Malheiros, Denise M. C. Schor, Nestor [UNIFESP] Yu, Luis Burdmann, Emmanuel A. |
author_role |
author |
author2 |
Abdulkader, Regina C. R. M. Barbaro, Katia C. Mendes, Gloria E. Castro, Isac Baptista, Maria A. S. F. Cury, Patricia M. Malheiros, Denise M. C. Schor, Nestor [UNIFESP] Yu, Luis Burdmann, Emmanuel A. |
author2_role |
author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Butantan Inst |
dc.contributor.author.fl_str_mv |
Lucato Junior, Rui Vicente [UNIFESP] Abdulkader, Regina C. R. M. Barbaro, Katia C. Mendes, Gloria E. Castro, Isac Baptista, Maria A. S. F. Cury, Patricia M. Malheiros, Denise M. C. Schor, Nestor [UNIFESP] Yu, Luis Burdmann, Emmanuel A. |
description |
Background: Accidents caused by Loxosceles spider may cause severe systemic reactions, including acute kidney injury (AKI). There are few experimental studies assessing Loxosceles venom effects on kidney function in vivo.Methodology/Principal Findings: in order to test Loxosceles gaucho venom (LV) nephrotoxicity and to assess some of the possible mechanisms of renal injury, rats were studied up to 60 minutes after LV 0.24 mg/kg or saline IV injection (control). LV caused a sharp and significant drop in glomerular filtration rate, renal blood flow and urinary output and increased renal vascular resistance, without changing blood pressure. Venom infusion increased significantly serum creatine kinase and aspartate aminotransferase. in the LV group renal histology analysis found acute epithelial tubular cells degenerative changes, presence of cell debris and detached epithelial cells in tubular lumen without glomerular or vascular changes. Immunohistochemistry disclosed renal deposition of myoglobin and hemoglobin. LV did not cause injury to a suspension of fresh proximal tubules isolated from rats.Conclusions/Significance: Loxosceles gaucho venom injection caused early AKI, which occurred without blood pressure variation. Changes in glomerular function occurred likely due to renal vasoconstriction and rhabdomyolysis. Direct nephrotoxicity could not be demonstrated in vitro. the development of a consistent model of Loxosceles venom-induced AKI and a better understanding of the mechanisms involved in the renal injury may allow more efficient ways to prevent or attenuate the systemic injury after Loxosceles bite. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-05-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:16:40Z |
dc.date.available.fl_str_mv |
2016-01-24T14:16:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 5, n. 5, 5 p., 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33658 http://dx.doi.org/10.1371/journal.pntd.0001182 |
dc.identifier.issn.none.fl_str_mv |
1935-2727 |
dc.identifier.file.none.fl_str_mv |
WOS000291099100043.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0001182 |
dc.identifier.wos.none.fl_str_mv |
WOS:000291099100043 |
identifier_str_mv |
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 5, n. 5, 5 p., 2011. 1935-2727 WOS000291099100043.pdf 10.1371/journal.pntd.0001182 WOS:000291099100043 |
url |
http://repositorio.unifesp.br/handle/11600/33658 http://dx.doi.org/10.1371/journal.pntd.0001182 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Plos Neglected Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/33658/1/WOS000291099100043.pdf ${dspace.ui.url}/bitstream/11600/33658/2/WOS000291099100043.pdf.txt |
bitstream.checksum.fl_str_mv |
94f277d447dedaa78947f9ff4c33b626 ad8287a1e5309cadba5b40da3b77007b |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764179569901568 |