Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.toxicon.2012.10.016 http://repositorio.unifesp.br/handle/11600/35656 |
Resumo: | The subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. the marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. the transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution. (c) 2012 Elsevier B.V. All rights reserved. |
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Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glandsAntimicrobial peptidesSkin gland secretionToxinsFrogThe subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. the marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. the transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution. (c) 2012 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo UNIFESP, Dept Farmacol, BR-04044020 São Paulo, BrazilInst Butantan, Ctr Appl Toxinol CAT CEPID, São Paulo, BrazilUniv Fed Ceara, Inst Ciencias Mar Labomar, Fortaleza, Ceara, BrazilInst Butantan, Lab Biol Celular, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Farmacol, BR-04044020 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Inst ButantanUniv Fed CearaNeiva, Márcia [UNIFESP]Vargas, D. C.Conceicao, K.Radis-Baptista, G.Assakura, M. T.Jared, Carlos [UNIFESP]Hayashi, Mirian Akemi Furuie [UNIFESP]2016-01-24T14:30:49Z2016-01-24T14:30:49Z2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion139-150application/pdfhttp://dx.doi.org/10.1016/j.toxicon.2012.10.016Toxicon. Oxford: Pergamon-Elsevier B.V., v. 61, p. 139-150, 2013.10.1016/j.toxicon.2012.10.016WOS000314146400017.pdf0041-0101http://repositorio.unifesp.br/handle/11600/35656WOS:000314146400017engToxiconinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T04:23:10Zoai:repositorio.unifesp.br/:11600/35656Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T04:23:10Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands |
title |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands |
spellingShingle |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands Neiva, Márcia [UNIFESP] Antimicrobial peptides Skin gland secretion Toxins Frog |
title_short |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands |
title_full |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands |
title_fullStr |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands |
title_full_unstemmed |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands |
title_sort |
Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands |
author |
Neiva, Márcia [UNIFESP] |
author_facet |
Neiva, Márcia [UNIFESP] Vargas, D. C. Conceicao, K. Radis-Baptista, G. Assakura, M. T. Jared, Carlos [UNIFESP] Hayashi, Mirian Akemi Furuie [UNIFESP] |
author_role |
author |
author2 |
Vargas, D. C. Conceicao, K. Radis-Baptista, G. Assakura, M. T. Jared, Carlos [UNIFESP] Hayashi, Mirian Akemi Furuie [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Inst Butantan Univ Fed Ceara |
dc.contributor.author.fl_str_mv |
Neiva, Márcia [UNIFESP] Vargas, D. C. Conceicao, K. Radis-Baptista, G. Assakura, M. T. Jared, Carlos [UNIFESP] Hayashi, Mirian Akemi Furuie [UNIFESP] |
dc.subject.por.fl_str_mv |
Antimicrobial peptides Skin gland secretion Toxins Frog |
topic |
Antimicrobial peptides Skin gland secretion Toxins Frog |
description |
The subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. the marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. the transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution. (c) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 2016-01-24T14:30:49Z 2016-01-24T14:30:49Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.toxicon.2012.10.016 Toxicon. Oxford: Pergamon-Elsevier B.V., v. 61, p. 139-150, 2013. 10.1016/j.toxicon.2012.10.016 WOS000314146400017.pdf 0041-0101 http://repositorio.unifesp.br/handle/11600/35656 WOS:000314146400017 |
url |
http://dx.doi.org/10.1016/j.toxicon.2012.10.016 http://repositorio.unifesp.br/handle/11600/35656 |
identifier_str_mv |
Toxicon. Oxford: Pergamon-Elsevier B.V., v. 61, p. 139-150, 2013. 10.1016/j.toxicon.2012.10.016 WOS000314146400017.pdf 0041-0101 WOS:000314146400017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicon |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
139-150 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268408494030848 |