Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands

Detalhes bibliográficos
Autor(a) principal: Neiva, Márcia [UNIFESP]
Data de Publicação: 2013
Outros Autores: Vargas, D. C., Conceicao, K., Radis-Baptista, G., Assakura, M. T., Jared, Carlos [UNIFESP], Hayashi, Mirian Akemi Furuie [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.toxicon.2012.10.016
http://repositorio.unifesp.br/handle/11600/35656
Resumo: The subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. the marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. the transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution. (c) 2012 Elsevier B.V. All rights reserved.
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spelling Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glandsAntimicrobial peptidesSkin gland secretionToxinsFrogThe subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. the marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. the transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution. (c) 2012 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo UNIFESP, Dept Farmacol, BR-04044020 São Paulo, BrazilInst Butantan, Ctr Appl Toxinol CAT CEPID, São Paulo, BrazilUniv Fed Ceara, Inst Ciencias Mar Labomar, Fortaleza, Ceara, BrazilInst Butantan, Lab Biol Celular, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Farmacol, BR-04044020 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Inst ButantanUniv Fed CearaNeiva, Márcia [UNIFESP]Vargas, D. C.Conceicao, K.Radis-Baptista, G.Assakura, M. T.Jared, Carlos [UNIFESP]Hayashi, Mirian Akemi Furuie [UNIFESP]2016-01-24T14:30:49Z2016-01-24T14:30:49Z2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion139-150application/pdfhttp://dx.doi.org/10.1016/j.toxicon.2012.10.016Toxicon. Oxford: Pergamon-Elsevier B.V., v. 61, p. 139-150, 2013.10.1016/j.toxicon.2012.10.016WOS000314146400017.pdf0041-0101http://repositorio.unifesp.br/handle/11600/35656WOS:000314146400017engToxiconinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T04:23:10Zoai:repositorio.unifesp.br/:11600/35656Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T04:23:10Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
title Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
spellingShingle Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
Neiva, Márcia [UNIFESP]
Antimicrobial peptides
Skin gland secretion
Toxins
Frog
title_short Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
title_full Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
title_fullStr Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
title_full_unstemmed Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
title_sort Gene expression analysis by ESTs sequencing of the Brazilian frog Phyllomedusa nordestina skin glands
author Neiva, Márcia [UNIFESP]
author_facet Neiva, Márcia [UNIFESP]
Vargas, D. C.
Conceicao, K.
Radis-Baptista, G.
Assakura, M. T.
Jared, Carlos [UNIFESP]
Hayashi, Mirian Akemi Furuie [UNIFESP]
author_role author
author2 Vargas, D. C.
Conceicao, K.
Radis-Baptista, G.
Assakura, M. T.
Jared, Carlos [UNIFESP]
Hayashi, Mirian Akemi Furuie [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Univ Fed Ceara
dc.contributor.author.fl_str_mv Neiva, Márcia [UNIFESP]
Vargas, D. C.
Conceicao, K.
Radis-Baptista, G.
Assakura, M. T.
Jared, Carlos [UNIFESP]
Hayashi, Mirian Akemi Furuie [UNIFESP]
dc.subject.por.fl_str_mv Antimicrobial peptides
Skin gland secretion
Toxins
Frog
topic Antimicrobial peptides
Skin gland secretion
Toxins
Frog
description The subfamily Phyllomedusinae has attracted a great interest of many researchers mainly due to the high diversity of these frog species and plethora of pharmacological activities frequently observed for their skin secretions. Despite of this fact, mainly for new species, limited information is available regarding the molecular composition of these skin secretions and the cellular components involved in their production. Phyllomedusa nordestina is a recently described Brazilian frog species also popularly known as 'tree-frogs'. Aiming at contributing to the biological knowledge of this species, we show here the gene expression profile of this frog skin secretion using a global ESTs analysis of a cDNA library. the marked aspect of this analysis revealed a significant higher transcriptional level of the opioid peptide dermorphins in P. nordestina skin secretion than in Phyllomedusa hypochondrialis, which is its closest related species, belonging both to the same phylogenetic group. Precursors of bioactive peptides as dermaseptins, phylloseptins, tryptophyllins, and bradykinin-like peptideswere also found in this library. Transcripts encoding proteins related to ordinary cellular functions and pathways were also described. Some of them are chiefly involved in the production of the skin secretion. Taken together, the data reported here constitute a contribution to the characterization of the molecular diversity of gene-encoded polypeptides with potential possibility of pharmacological exploitation. the transcriptional composition of the skin secretion may also help to give the necessary support for the definition of P. nordestina as a new species, which actually relies basically on frog morphological characteristics and geographical distribution. (c) 2012 Elsevier B.V. All rights reserved.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
2016-01-24T14:30:49Z
2016-01-24T14:30:49Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.toxicon.2012.10.016
Toxicon. Oxford: Pergamon-Elsevier B.V., v. 61, p. 139-150, 2013.
10.1016/j.toxicon.2012.10.016
WOS000314146400017.pdf
0041-0101
http://repositorio.unifesp.br/handle/11600/35656
WOS:000314146400017
url http://dx.doi.org/10.1016/j.toxicon.2012.10.016
http://repositorio.unifesp.br/handle/11600/35656
identifier_str_mv Toxicon. Oxford: Pergamon-Elsevier B.V., v. 61, p. 139-150, 2013.
10.1016/j.toxicon.2012.10.016
WOS000314146400017.pdf
0041-0101
WOS:000314146400017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicon
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 139-150
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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