Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: D'Azevedo, Pedro Alves [UNIFESP]
Data de Publicação: 2008
Outros Autores: Furtado, Guilherme Henrique Campos [UNIFESP], Medeiros, Eduardo Alexandrino Servolo de [UNIFESP], Santiago, Kelly Aline de Souza [UNIFESP], Silbert, Suzane [UNIFESP], Pignatari, Antonio Carlos Campos [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0036-46652008000400001
http://repositorio.unifesp.br/handle/11600/4489
Resumo: E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.
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spelling Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, BrazilCaracterização molecular de espécies de Enterococci resistentes à vancomicina oito anos após seu primeiro isolamento em São Paulo, BrasilVREE. faecalisE. faeciumPFGE (Pulsed-Field Gel Electrophoresis)E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.E. faecium contendo o gene vanA foi a primeira espécie de VRE descrita, no Brasil. Apesar disto, E. faecalis resistente a vancomicina tem se tornado a espécie predominante nos hospitais brasileiros.O objetivo desse estudo foi avaliar a relação genética de VREs isolados em um hospital de ensino brasileiro após oito anos de seu primeiro isolamento. Analisamos 37 isolados de VRE obtidos de 81 culturas de vigilância de pacientes admitidos nas quatro maiores Unidades de Tratamento Intensivo em Fevereiro de 2006. A presença do gene vanA foi analisada por PCR e a caracterização molecular por PFGE. Todas as amostras VRE carreavam o gene vanA. Entre os E. faecalis vancomicina-resistentes, dois distintos grupos clonais foram observados. E. faecium resistente a vancomicina pertencentes a cinco clones distintos foram demonstrados por tipagem molecular. Todos esses clones foram diferentes do primeiro clone de enterococo resistente a vancomicina isolado oito anos atrás em nosso hospital.Universidade Federal de São Paulo (UNIFESP) Laboratório Especial de Microbiologia ClínicaFundação Faculdade Federal de Porto Alegre Departamento de Microbiologia e ParasitologiaUNIFESP Hospital São Paulo Comissão de Epidemiologia HospitalarUNIFESP, Laboratório Especial de Microbiologia ClínicaUNIFESP, Hospital São Paulo Comissão de Epidemiologia HospitalarSciELOConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto de Medicina TropicalUniversidade Federal de São Paulo (UNIFESP)Fundação Faculdade Federal de Porto Alegre Departamento de Microbiologia e ParasitologiaD'Azevedo, Pedro Alves [UNIFESP]Furtado, Guilherme Henrique Campos [UNIFESP]Medeiros, Eduardo Alexandrino Servolo de [UNIFESP]Santiago, Kelly Aline de Souza [UNIFESP]Silbert, Suzane [UNIFESP]Pignatari, Antonio Carlos Campos [UNIFESP]2015-06-14T13:38:39Z2015-06-14T13:38:39Z2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion195-198application/pdfhttp://dx.doi.org/10.1590/S0036-46652008000400001D'AZEVEDO, Pedro Alves et al . Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil. Rev. Inst. Med. trop. S. Paulo, São Paulo , v. 50, n. 4, p. 195-198, ago. 200810.1590/S0036-46652008000400001S0036-46652008000400001.pdf0036-4665S0036-46652008000400001http://repositorio.unifesp.br/handle/11600/4489WOS:000259153900001engRevista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T13:32:16Zoai:repositorio.unifesp.br/:11600/4489Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T13:32:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
Caracterização molecular de espécies de Enterococci resistentes à vancomicina oito anos após seu primeiro isolamento em São Paulo, Brasil
title Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
spellingShingle Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
D'Azevedo, Pedro Alves [UNIFESP]
VRE
E. faecalis
E. faecium
PFGE (Pulsed-Field Gel Electrophoresis)
title_short Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
title_full Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
title_fullStr Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
title_full_unstemmed Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
title_sort Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil
author D'Azevedo, Pedro Alves [UNIFESP]
author_facet D'Azevedo, Pedro Alves [UNIFESP]
Furtado, Guilherme Henrique Campos [UNIFESP]
Medeiros, Eduardo Alexandrino Servolo de [UNIFESP]
Santiago, Kelly Aline de Souza [UNIFESP]
Silbert, Suzane [UNIFESP]
Pignatari, Antonio Carlos Campos [UNIFESP]
author_role author
author2 Furtado, Guilherme Henrique Campos [UNIFESP]
Medeiros, Eduardo Alexandrino Servolo de [UNIFESP]
Santiago, Kelly Aline de Souza [UNIFESP]
Silbert, Suzane [UNIFESP]
Pignatari, Antonio Carlos Campos [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Fundação Faculdade Federal de Porto Alegre Departamento de Microbiologia e Parasitologia
dc.contributor.author.fl_str_mv D'Azevedo, Pedro Alves [UNIFESP]
Furtado, Guilherme Henrique Campos [UNIFESP]
Medeiros, Eduardo Alexandrino Servolo de [UNIFESP]
Santiago, Kelly Aline de Souza [UNIFESP]
Silbert, Suzane [UNIFESP]
Pignatari, Antonio Carlos Campos [UNIFESP]
dc.subject.por.fl_str_mv VRE
E. faecalis
E. faecium
PFGE (Pulsed-Field Gel Electrophoresis)
topic VRE
E. faecalis
E. faecium
PFGE (Pulsed-Field Gel Electrophoresis)
description E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
2015-06-14T13:38:39Z
2015-06-14T13:38:39Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0036-46652008000400001
D'AZEVEDO, Pedro Alves et al . Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil. Rev. Inst. Med. trop. S. Paulo, São Paulo , v. 50, n. 4, p. 195-198, ago. 2008
10.1590/S0036-46652008000400001
S0036-46652008000400001.pdf
0036-4665
S0036-46652008000400001
http://repositorio.unifesp.br/handle/11600/4489
WOS:000259153900001
url http://dx.doi.org/10.1590/S0036-46652008000400001
http://repositorio.unifesp.br/handle/11600/4489
identifier_str_mv D'AZEVEDO, Pedro Alves et al . Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil. Rev. Inst. Med. trop. S. Paulo, São Paulo , v. 50, n. 4, p. 195-198, ago. 2008
10.1590/S0036-46652008000400001
S0036-46652008000400001.pdf
0036-4665
S0036-46652008000400001
WOS:000259153900001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 195-198
application/pdf
dc.publisher.none.fl_str_mv Instituto de Medicina Tropical
publisher.none.fl_str_mv Instituto de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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