Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil

Detalhes bibliográficos
Autor(a) principal: Riccio, Evelyn K. P.
Data de Publicação: 2013
Outros Autores: Totino, Paulo R. R., Pratt-Riccio, Lilian R., Ennes-Vidal, Vitor, Soares, Irene S., Rodrigues, Mauricio Martins [UNIFESP], Souza, Jose Maria de, Daniel-Ribeiro, Claudio Tadeu, Ferreira-da-Cruz, Maria de Fatima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1186/1475-2875-12-326
http://repositorio.unifesp.br/handle/11600/36757
Resumo: Background: Plasmodium vivax merozoite surface protein-1 (MSP-1) is an antigen considered to be one of the leading malaria vaccine candidates. PvMSP-1 is highly immunogenic and evidences suggest that it is target for protective immunity against asexual blood stages of malaria parasites. Thus, this study aims to evaluate the acquired cellular and antibody immune responses against PvMSP-1 in individuals naturally exposed to malaria infections in a malaria-endemic area in the north-eastern Amazon region of Brazil.Methods: the study was carried out in Paragominas, Para State, in the Brazilian Amazon. Blood samples were collected from 35 individuals with uncomplicated malaria. Peripheral blood mononuclear cells were isolated and the cellular proliferation and activation was analysed in presence of 19 kDa fragment of MSP-1 (PvMSP-1(19)) and Plasmodium falciparum PSS1 crude antigen. Antibodies IgE, IgM, IgG and IgG subclass and the levels of TNF, IFN-gamma and IL-10 were measured by enzyme-linked immunosorbent assay.Results: the prevalence of activated CD4(+) was greater than CD8(+) T cells, in both ex-vivo and in 96 h culture in presence of PvMSP-1(19) and PSS1 antigen. A low proliferative response against PvMSP-1(19) and PSS1 crude antigen after 96 h culture was observed. High plasmatic levels of IFN-gamma and IL-10 as well as lower TNF levels were also detected in malaria patients. However, in the 96 h supernatant culture, the dynamics of cytokine responses differed from those depicted on plasma assays; in presence of PvMSP-1(19) stimulus, higher levels of TNF were noted in supernatant 96 h culture of malaria patient's cells while low levels of IFN-gamma and IL-10 were verified. High frequency of malaria patients presenting antibodies against PvMSP-1(19) was evidenced, regardless class or IgG subclass. PvMSP-1(19)-induced antibodies were predominantly on non-cytophilic subclasses.Conclusions: the results presented here shows that PvMSP-1(19) was able to induce a high cellular activation, leading to production of TNF and emphasizes the high immunogenicity of PvMSP-1(19) in naturally exposed individuals and, therefore, its potential as a malaria vaccine candidate.
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spelling Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of BrazilMalariaPlasmodium vivaxMSP-1(19)Cellular responseAntibodiesBackground: Plasmodium vivax merozoite surface protein-1 (MSP-1) is an antigen considered to be one of the leading malaria vaccine candidates. PvMSP-1 is highly immunogenic and evidences suggest that it is target for protective immunity against asexual blood stages of malaria parasites. Thus, this study aims to evaluate the acquired cellular and antibody immune responses against PvMSP-1 in individuals naturally exposed to malaria infections in a malaria-endemic area in the north-eastern Amazon region of Brazil.Methods: the study was carried out in Paragominas, Para State, in the Brazilian Amazon. Blood samples were collected from 35 individuals with uncomplicated malaria. Peripheral blood mononuclear cells were isolated and the cellular proliferation and activation was analysed in presence of 19 kDa fragment of MSP-1 (PvMSP-1(19)) and Plasmodium falciparum PSS1 crude antigen. Antibodies IgE, IgM, IgG and IgG subclass and the levels of TNF, IFN-gamma and IL-10 were measured by enzyme-linked immunosorbent assay.Results: the prevalence of activated CD4(+) was greater than CD8(+) T cells, in both ex-vivo and in 96 h culture in presence of PvMSP-1(19) and PSS1 antigen. A low proliferative response against PvMSP-1(19) and PSS1 crude antigen after 96 h culture was observed. High plasmatic levels of IFN-gamma and IL-10 as well as lower TNF levels were also detected in malaria patients. However, in the 96 h supernatant culture, the dynamics of cytokine responses differed from those depicted on plasma assays; in presence of PvMSP-1(19) stimulus, higher levels of TNF were noted in supernatant 96 h culture of malaria patient's cells while low levels of IFN-gamma and IL-10 were verified. High frequency of malaria patients presenting antibodies against PvMSP-1(19) was evidenced, regardless class or IgG subclass. PvMSP-1(19)-induced antibodies were predominantly on non-cytophilic subclasses.Conclusions: the results presented here shows that PvMSP-1(19) was able to induce a high cellular activation, leading to production of TNF and emphasizes the high immunogenicity of PvMSP-1(19) in naturally exposed individuals and, therefore, its potential as a malaria vaccine candidate.Fiocruz MS, Inst Oswaldo Cruz, Lab Pesquisas Malaria, BR-21040900 Rio de Janeiro, RJ, BrazilFiocruz MS, Ctr Pesquisa Diagnost & Treinamento Malaria CPD M, Reference Ctr Malaria Extra Amazonian Reg Secreta, BR-21040900 Rio de Janeiro, RJ, BrazilFiocruz MS, Inst Oswaldo Cruz, Lab Biol Mol & Doencas Endem, BR-21040900 Rio de Janeiro, RJ, BrazilUniv São Paulo, Dept Analises Clin & Toxicol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilSVS, Inst Evandro Chagas, Programa Ensaios Clin Malaria, Belem, Para, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto Oswaldo Cruz (FIOCRUZ, Brazil)Biomed Central LtdFiocruz MSUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)SVSRiccio, Evelyn K. P.Totino, Paulo R. R.Pratt-Riccio, Lilian R.Ennes-Vidal, VitorSoares, Irene S.Rodrigues, Mauricio Martins [UNIFESP]Souza, Jose Maria deDaniel-Ribeiro, Claudio TadeuFerreira-da-Cruz, Maria de Fatima2016-01-24T14:34:26Z2016-01-24T14:34:26Z2013-09-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15application/pdfhttp://dx.doi.org/10.1186/1475-2875-12-326Malaria Journal. London: Biomed Central Ltd, v. 12, 15 p., 2013.10.1186/1475-2875-12-326WOS000329093500001.pdf1475-2875http://repositorio.unifesp.br/handle/11600/36757WOS:000329093500001engMalaria Journalinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T15:29:27Zoai:repositorio.unifesp.br/:11600/36757Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T15:29:27Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
title Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
spellingShingle Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
Riccio, Evelyn K. P.
Malaria
Plasmodium vivax
MSP-1(19)
Cellular response
Antibodies
title_short Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
title_full Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
title_fullStr Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
title_full_unstemmed Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
title_sort Cellular and humoral immune responses against the Plasmodium vivax MSP-1(19) malaria vaccine candidate in individuals living in an endemic area in north-eastern Amazon region of Brazil
author Riccio, Evelyn K. P.
author_facet Riccio, Evelyn K. P.
Totino, Paulo R. R.
Pratt-Riccio, Lilian R.
Ennes-Vidal, Vitor
Soares, Irene S.
Rodrigues, Mauricio Martins [UNIFESP]
Souza, Jose Maria de
Daniel-Ribeiro, Claudio Tadeu
Ferreira-da-Cruz, Maria de Fatima
author_role author
author2 Totino, Paulo R. R.
Pratt-Riccio, Lilian R.
Ennes-Vidal, Vitor
Soares, Irene S.
Rodrigues, Mauricio Martins [UNIFESP]
Souza, Jose Maria de
Daniel-Ribeiro, Claudio Tadeu
Ferreira-da-Cruz, Maria de Fatima
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fiocruz MS
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
SVS
dc.contributor.author.fl_str_mv Riccio, Evelyn K. P.
Totino, Paulo R. R.
Pratt-Riccio, Lilian R.
Ennes-Vidal, Vitor
Soares, Irene S.
Rodrigues, Mauricio Martins [UNIFESP]
Souza, Jose Maria de
Daniel-Ribeiro, Claudio Tadeu
Ferreira-da-Cruz, Maria de Fatima
dc.subject.por.fl_str_mv Malaria
Plasmodium vivax
MSP-1(19)
Cellular response
Antibodies
topic Malaria
Plasmodium vivax
MSP-1(19)
Cellular response
Antibodies
description Background: Plasmodium vivax merozoite surface protein-1 (MSP-1) is an antigen considered to be one of the leading malaria vaccine candidates. PvMSP-1 is highly immunogenic and evidences suggest that it is target for protective immunity against asexual blood stages of malaria parasites. Thus, this study aims to evaluate the acquired cellular and antibody immune responses against PvMSP-1 in individuals naturally exposed to malaria infections in a malaria-endemic area in the north-eastern Amazon region of Brazil.Methods: the study was carried out in Paragominas, Para State, in the Brazilian Amazon. Blood samples were collected from 35 individuals with uncomplicated malaria. Peripheral blood mononuclear cells were isolated and the cellular proliferation and activation was analysed in presence of 19 kDa fragment of MSP-1 (PvMSP-1(19)) and Plasmodium falciparum PSS1 crude antigen. Antibodies IgE, IgM, IgG and IgG subclass and the levels of TNF, IFN-gamma and IL-10 were measured by enzyme-linked immunosorbent assay.Results: the prevalence of activated CD4(+) was greater than CD8(+) T cells, in both ex-vivo and in 96 h culture in presence of PvMSP-1(19) and PSS1 antigen. A low proliferative response against PvMSP-1(19) and PSS1 crude antigen after 96 h culture was observed. High plasmatic levels of IFN-gamma and IL-10 as well as lower TNF levels were also detected in malaria patients. However, in the 96 h supernatant culture, the dynamics of cytokine responses differed from those depicted on plasma assays; in presence of PvMSP-1(19) stimulus, higher levels of TNF were noted in supernatant 96 h culture of malaria patient's cells while low levels of IFN-gamma and IL-10 were verified. High frequency of malaria patients presenting antibodies against PvMSP-1(19) was evidenced, regardless class or IgG subclass. PvMSP-1(19)-induced antibodies were predominantly on non-cytophilic subclasses.Conclusions: the results presented here shows that PvMSP-1(19) was able to induce a high cellular activation, leading to production of TNF and emphasizes the high immunogenicity of PvMSP-1(19) in naturally exposed individuals and, therefore, its potential as a malaria vaccine candidate.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-16
2016-01-24T14:34:26Z
2016-01-24T14:34:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1475-2875-12-326
Malaria Journal. London: Biomed Central Ltd, v. 12, 15 p., 2013.
10.1186/1475-2875-12-326
WOS000329093500001.pdf
1475-2875
http://repositorio.unifesp.br/handle/11600/36757
WOS:000329093500001
url http://dx.doi.org/10.1186/1475-2875-12-326
http://repositorio.unifesp.br/handle/11600/36757
identifier_str_mv Malaria Journal. London: Biomed Central Ltd, v. 12, 15 p., 2013.
10.1186/1475-2875-12-326
WOS000329093500001.pdf
1475-2875
WOS:000329093500001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Malaria Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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