Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract

Detalhes bibliográficos
Autor(a) principal: Patrão, Marilia Tavares Coutinho da Costa [UNIFESP]
Data de Publicação: 2008
Outros Autores: Queiroz, Daniel Barboza Cava [UNIFESP], Grossman, Gail, Petrusz, Peter, Lazari, Maria de Fatima Magalhaes [UNIFESP], Avellar, Maria Christina Werneck [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1093/molehr/gam084
https://repositorio.unifesp.br/handle/11600/30391
Resumo: This study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys.
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spelling Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tractalpha(1)-adrenoceptorHumanMale reproductive tractRhesus monkeySplice variantsThis study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys.Universidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, BrazilUniv N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USAUniv N Carolina, Labs Reprod Biol, Chapel Hill, NC 27599 USAUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, BrazilWeb of ScienceOxford Univ PressUniversidade Federal de São Paulo (UNIFESP)Univ N CarolinaPatrão, Marilia Tavares Coutinho da Costa [UNIFESP]Queiroz, Daniel Barboza Cava [UNIFESP]Grossman, GailPetrusz, PeterLazari, Maria de Fatima Magalhaes [UNIFESP]Avellar, Maria Christina Werneck [UNIFESP]2016-01-24T13:49:30Z2016-01-24T13:49:30Z2008-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion85-96https://dx.doi.org/10.1093/molehr/gam084Molecular Human Reproduction. Oxford: Oxford Univ Press, v. 14, n. 2, p. 85-96, 2008.10.1093/molehr/gam0841360-9947https://repositorio.unifesp.br/handle/11600/30391WOS:000257148600003engMolecular Human Reproductioninfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-11-03T16:22:04Zoai:repositorio.unifesp.br/:11600/30391Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-11-03T16:22:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
title Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
spellingShingle Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
Patrão, Marilia Tavares Coutinho da Costa [UNIFESP]
alpha(1)-adrenoceptor
Human
Male reproductive tract
Rhesus monkey
Splice variants
title_short Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
title_full Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
title_fullStr Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
title_full_unstemmed Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
title_sort Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
author Patrão, Marilia Tavares Coutinho da Costa [UNIFESP]
author_facet Patrão, Marilia Tavares Coutinho da Costa [UNIFESP]
Queiroz, Daniel Barboza Cava [UNIFESP]
Grossman, Gail
Petrusz, Peter
Lazari, Maria de Fatima Magalhaes [UNIFESP]
Avellar, Maria Christina Werneck [UNIFESP]
author_role author
author2 Queiroz, Daniel Barboza Cava [UNIFESP]
Grossman, Gail
Petrusz, Peter
Lazari, Maria de Fatima Magalhaes [UNIFESP]
Avellar, Maria Christina Werneck [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Univ N Carolina
dc.contributor.author.fl_str_mv Patrão, Marilia Tavares Coutinho da Costa [UNIFESP]
Queiroz, Daniel Barboza Cava [UNIFESP]
Grossman, Gail
Petrusz, Peter
Lazari, Maria de Fatima Magalhaes [UNIFESP]
Avellar, Maria Christina Werneck [UNIFESP]
dc.subject.por.fl_str_mv alpha(1)-adrenoceptor
Human
Male reproductive tract
Rhesus monkey
Splice variants
topic alpha(1)-adrenoceptor
Human
Male reproductive tract
Rhesus monkey
Splice variants
description This study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys.
publishDate 2008
dc.date.none.fl_str_mv 2008-02-01
2016-01-24T13:49:30Z
2016-01-24T13:49:30Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1093/molehr/gam084
Molecular Human Reproduction. Oxford: Oxford Univ Press, v. 14, n. 2, p. 85-96, 2008.
10.1093/molehr/gam084
1360-9947
https://repositorio.unifesp.br/handle/11600/30391
WOS:000257148600003
url https://dx.doi.org/10.1093/molehr/gam084
https://repositorio.unifesp.br/handle/11600/30391
identifier_str_mv Molecular Human Reproduction. Oxford: Oxford Univ Press, v. 14, n. 2, p. 85-96, 2008.
10.1093/molehr/gam084
1360-9947
WOS:000257148600003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecular Human Reproduction
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.format.none.fl_str_mv 85-96
dc.publisher.none.fl_str_mv Oxford Univ Press
publisher.none.fl_str_mv Oxford Univ Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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