Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1093/molehr/gam084 https://repositorio.unifesp.br/handle/11600/30391 |
Resumo: | This study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys. |
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Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tractalpha(1)-adrenoceptorHumanMale reproductive tractRhesus monkeySplice variantsThis study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys.Universidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, BrazilUniv N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USAUniv N Carolina, Labs Reprod Biol, Chapel Hill, NC 27599 USAUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, BrazilWeb of ScienceOxford Univ PressUniversidade Federal de São Paulo (UNIFESP)Univ N CarolinaPatrão, Marilia Tavares Coutinho da Costa [UNIFESP]Queiroz, Daniel Barboza Cava [UNIFESP]Grossman, GailPetrusz, PeterLazari, Maria de Fatima Magalhaes [UNIFESP]Avellar, Maria Christina Werneck [UNIFESP]2016-01-24T13:49:30Z2016-01-24T13:49:30Z2008-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion85-96https://dx.doi.org/10.1093/molehr/gam084Molecular Human Reproduction. Oxford: Oxford Univ Press, v. 14, n. 2, p. 85-96, 2008.10.1093/molehr/gam0841360-9947https://repositorio.unifesp.br/handle/11600/30391WOS:000257148600003engMolecular Human Reproductioninfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-11-03T16:22:04Zoai:repositorio.unifesp.br/:11600/30391Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-11-03T16:22:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract |
title |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract |
spellingShingle |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract Patrão, Marilia Tavares Coutinho da Costa [UNIFESP] alpha(1)-adrenoceptor Human Male reproductive tract Rhesus monkey Splice variants |
title_short |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract |
title_full |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract |
title_fullStr |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract |
title_full_unstemmed |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract |
title_sort |
Cloning, expression and immunolocalization of alpha(1)-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract |
author |
Patrão, Marilia Tavares Coutinho da Costa [UNIFESP] |
author_facet |
Patrão, Marilia Tavares Coutinho da Costa [UNIFESP] Queiroz, Daniel Barboza Cava [UNIFESP] Grossman, Gail Petrusz, Peter Lazari, Maria de Fatima Magalhaes [UNIFESP] Avellar, Maria Christina Werneck [UNIFESP] |
author_role |
author |
author2 |
Queiroz, Daniel Barboza Cava [UNIFESP] Grossman, Gail Petrusz, Peter Lazari, Maria de Fatima Magalhaes [UNIFESP] Avellar, Maria Christina Werneck [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ N Carolina |
dc.contributor.author.fl_str_mv |
Patrão, Marilia Tavares Coutinho da Costa [UNIFESP] Queiroz, Daniel Barboza Cava [UNIFESP] Grossman, Gail Petrusz, Peter Lazari, Maria de Fatima Magalhaes [UNIFESP] Avellar, Maria Christina Werneck [UNIFESP] |
dc.subject.por.fl_str_mv |
alpha(1)-adrenoceptor Human Male reproductive tract Rhesus monkey Splice variants |
topic |
alpha(1)-adrenoceptor Human Male reproductive tract Rhesus monkey Splice variants |
description |
This study reports the genomic organization of the rhesus alpha(1A)-adrenoceptor gene (ADRA1A). Full-length cloning of rhesus ADRA1A splice variants was achieved by combining PCR screening of a seminal vesicle cDNA library and 5'-RACE assays with total RNA from seminal vesicle. the classical ADRA1A mRNA (ADRA1A_nu 1) and six full-length ADRA1A splice variants were identified representing transcripts that code for functional (ADRA1A_nu 1, ADRA1A_nu 2a, ADRA1A_nu 3a, ADRA1A_nu 3d, ADRA1A_nu 3e) and truncated (ADRA1A_nu 2c and ADRA1A_nu 3c) receptor isoforms. Comparative analysis of the deduced amino acid sequence indicated that rhesus ADRA1A_i1 isoform (corresponding to the ADRA1A_nu 1 transcript) shares high identity to the amino acid sequence present in the classical alpha(1A)-adrenoceptor from human and other mammalian species. Partial nucleotide sequences for rhesus alpha(1B)-(ADRA1B) and alpha(1D)-adrenoceptor (ADRA1D) transcripts were also characterized. RT-PCR studies indicated differential distribution of all ADRA1A-related splice variants as well as ADRA1B and ADRA1D mRNAs, in tissues from rhesus and human male reproductive tract. Immunohistochemistry revealed alpha(1A)-adrenoceptor (ADRA1A_ i1) immunostaining in smooth muscle cells and epithelial cells of rhesus efferent ductules, epididymis and seminal vesicle. Taken together the present results demonstrate that the complexity of the splicing mechanisms involved in the regulation of the ADRA1A gene is not restricted to human and is a common characteristic among Old World monkeys. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-02-01 2016-01-24T13:49:30Z 2016-01-24T13:49:30Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1093/molehr/gam084 Molecular Human Reproduction. Oxford: Oxford Univ Press, v. 14, n. 2, p. 85-96, 2008. 10.1093/molehr/gam084 1360-9947 https://repositorio.unifesp.br/handle/11600/30391 WOS:000257148600003 |
url |
https://dx.doi.org/10.1093/molehr/gam084 https://repositorio.unifesp.br/handle/11600/30391 |
identifier_str_mv |
Molecular Human Reproduction. Oxford: Oxford Univ Press, v. 14, n. 2, p. 85-96, 2008. 10.1093/molehr/gam084 1360-9947 WOS:000257148600003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Human Reproduction |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
dc.format.none.fl_str_mv |
85-96 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268316920840192 |