Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37900 http://dx.doi.org/10.1128/EC.00066-14 |
Resumo: | The phosphorylation of the carboxy-terminal heptapeptide repeats of the largest subunit of RNA polymerase II (Pol II) controls several transcription-related events in eukaryotes. Trypanosomatids lack these typical repeats and display an unusual transcription control. RNA Pol II associates with the transcription site of the spliced leader (SL) RNA, which is used in the trans-splicing of all mRNAs transcribed on long polycistronic units. We found that Trypanosoma cruzi RNA Pol II associated with chromatin is highly phosphorylated. When transcription is inhibited by actinomycin D, the enzyme runs off from SL genes, remaining hyperphosphorylated and associated with polycistronic transcription units. Upon heat shock, the enzyme is dephosphorylated and remains associated with the chromatin. Transcription is partially inhibited with the accumulation of housekeeping precursor mRNAs, except for heat shock genes. DNA damage caused dephosphorylation and transcription arrest, with RNA Pol II dissociating from chromatin although staying at the SL. in the presence of calyculin A, the hyperphosphorylated form detached from chromatin, including the SL loci. These results indicate that in trypanosomes, the unusual RNA Pol II is phosphorylated during the transcription of SL and polycistronic operons. Different types of stresses modify its phosphorylation state, affecting pre-RNA processing. |
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Rocha, Antonio Augusto [UNIFESP]Moretti, Nilmar Silvio [UNIFESP]Schenkman, Sergio [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:37:29Z2016-01-24T14:37:29Z2014-07-01Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 13, n. 7, p. 855-865, 2014.1535-9778http://repositorio.unifesp.br/handle/11600/37900http://dx.doi.org/10.1128/EC.00066-14WOS000338767900002.pdf10.1128/EC.00066-14WOS:000338767900002The phosphorylation of the carboxy-terminal heptapeptide repeats of the largest subunit of RNA polymerase II (Pol II) controls several transcription-related events in eukaryotes. Trypanosomatids lack these typical repeats and display an unusual transcription control. RNA Pol II associates with the transcription site of the spliced leader (SL) RNA, which is used in the trans-splicing of all mRNAs transcribed on long polycistronic units. We found that Trypanosoma cruzi RNA Pol II associated with chromatin is highly phosphorylated. When transcription is inhibited by actinomycin D, the enzyme runs off from SL genes, remaining hyperphosphorylated and associated with polycistronic transcription units. Upon heat shock, the enzyme is dephosphorylated and remains associated with the chromatin. Transcription is partially inhibited with the accumulation of housekeeping precursor mRNAs, except for heat shock genes. DNA damage caused dephosphorylation and transcription arrest, with RNA Pol II dissociating from chromatin although staying at the SL. in the presence of calyculin A, the hyperphosphorylated form detached from chromatin, including the SL loci. These results indicate that in trypanosomes, the unusual RNA Pol II is phosphorylated during the transcription of SL and polycistronic operons. Different types of stresses modify its phosphorylation state, affecting pre-RNA processing.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilFAPESP: 2011/51973-3FAPESP: 2012/09327-0FAPESP: 2012/09403-8CNPq: 477143/2011-3Web of Science855-865engAmer Soc MicrobiologyEukaryotic CellStress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000338767900002.pdfapplication/pdf3350301${dspace.ui.url}/bitstream/11600/37900/1/WOS000338767900002.pdf4e178484a30af7d5cf2bfc5a315382d6MD51open accessTEXTWOS000338767900002.pdf.txtWOS000338767900002.pdf.txtExtracted texttext/plain67859${dspace.ui.url}/bitstream/11600/37900/2/WOS000338767900002.pdf.txt21b542cef352b92aede8523b67b4dec9MD52open access11600/379002022-06-02 09:05:58.863open accessoai:repositorio.unifesp.br:11600/37900Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:05:58Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing |
title |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing |
spellingShingle |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing Rocha, Antonio Augusto [UNIFESP] |
title_short |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing |
title_full |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing |
title_fullStr |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing |
title_full_unstemmed |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing |
title_sort |
Stress Induces Changes in the Phosphorylation of Trypanosoma cruzi RNA Polymerase II, Affecting Its Association with Chromatin and RNA Processing |
author |
Rocha, Antonio Augusto [UNIFESP] |
author_facet |
Rocha, Antonio Augusto [UNIFESP] Moretti, Nilmar Silvio [UNIFESP] Schenkman, Sergio [UNIFESP] |
author_role |
author |
author2 |
Moretti, Nilmar Silvio [UNIFESP] Schenkman, Sergio [UNIFESP] |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Rocha, Antonio Augusto [UNIFESP] Moretti, Nilmar Silvio [UNIFESP] Schenkman, Sergio [UNIFESP] |
description |
The phosphorylation of the carboxy-terminal heptapeptide repeats of the largest subunit of RNA polymerase II (Pol II) controls several transcription-related events in eukaryotes. Trypanosomatids lack these typical repeats and display an unusual transcription control. RNA Pol II associates with the transcription site of the spliced leader (SL) RNA, which is used in the trans-splicing of all mRNAs transcribed on long polycistronic units. We found that Trypanosoma cruzi RNA Pol II associated with chromatin is highly phosphorylated. When transcription is inhibited by actinomycin D, the enzyme runs off from SL genes, remaining hyperphosphorylated and associated with polycistronic transcription units. Upon heat shock, the enzyme is dephosphorylated and remains associated with the chromatin. Transcription is partially inhibited with the accumulation of housekeeping precursor mRNAs, except for heat shock genes. DNA damage caused dephosphorylation and transcription arrest, with RNA Pol II dissociating from chromatin although staying at the SL. in the presence of calyculin A, the hyperphosphorylated form detached from chromatin, including the SL loci. These results indicate that in trypanosomes, the unusual RNA Pol II is phosphorylated during the transcription of SL and polycistronic operons. Different types of stresses modify its phosphorylation state, affecting pre-RNA processing. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:37:29Z |
dc.date.available.fl_str_mv |
2016-01-24T14:37:29Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 13, n. 7, p. 855-865, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37900 http://dx.doi.org/10.1128/EC.00066-14 |
dc.identifier.issn.none.fl_str_mv |
1535-9778 |
dc.identifier.file.none.fl_str_mv |
WOS000338767900002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1128/EC.00066-14 |
dc.identifier.wos.none.fl_str_mv |
WOS:000338767900002 |
identifier_str_mv |
Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 13, n. 7, p. 855-865, 2014. 1535-9778 WOS000338767900002.pdf 10.1128/EC.00066-14 WOS:000338767900002 |
url |
http://repositorio.unifesp.br/handle/11600/37900 http://dx.doi.org/10.1128/EC.00066-14 |
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eng |
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Amer Soc Microbiology |
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Amer Soc Microbiology |
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