Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38486 http://dx.doi.org/10.1590/0074-0276140193 |
Resumo: | The interferon (IFN)-gamma response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4(+) T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-gamma enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-gamma production by PBMCs from at least 31% of the TST-positive donors. the magnitude of the response against all peptides was 182 +/- 230 x 10(6) IFN-gamma spot forming cells (SFC) among TST-positive donors and 36 +/- 62 x 10(6) SFC among TST-negative donors (p = 0.007). the response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-gamma assays to identify individuals with this condition. |
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Maciel da Silva, Bosco Christiano [UNIFESP]Rios Grassi, Maria FernandaCoutinho, RaimundoMoreira Mascarenhas, Rita ElizabethOlavarria, Viviana NillaCoutinho-Borgo, Adriana [UNIFESP]Kalil, Jorge [UNIFESP]Cunha-Neto, Edecio [UNIFESP]Fonseca, Simone Goncalves [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Fundacao Oswaldo CruzEscola Bahiana Med & Saude PublInst Invest Immunol2016-01-24T14:38:13Z2016-01-24T14:38:13Z2014-12-01Memorias Do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 109, n. 8, p. 999-1004, 2014.0074-0276http://repositorio.unifesp.br/handle/11600/38486http://dx.doi.org/10.1590/0074-0276140193S0074-02762014000700970.pdfS0074-0276201400070097010.1590/0074-0276140193WOS:000347528000005The interferon (IFN)-gamma response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4(+) T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-gamma enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-gamma production by PBMCs from at least 31% of the TST-positive donors. the magnitude of the response against all peptides was 182 +/- 230 x 10(6) IFN-gamma spot forming cells (SFC) among TST-positive donors and 36 +/- 62 x 10(6) SFC among TST-negative donors (p = 0.007). the response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-gamma assays to identify individuals with this condition.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Fac Med, Inst Coracao, Immunol Lab, São Paulo, BrazilUniversidade Federal de São Paulo, Fac Med, Dept Med, Div Imunol Clin & Alergia, São Paulo, BrazilFundacao Oswaldo Cruz, Lab Avancado Saude Publ, Salvador, BA, BrazilEscola Bahiana Med & Saude Publ, Salvador, BA, BrazilInst Invest Immunol, São Paulo, BrazilUniversidade Federal de São Paulo, Fac Med, Inst Coracao, Immunol Lab, São Paulo, BrazilUniversidade Federal de São Paulo, Fac Med, Dept Med, Div Imunol Clin & Alergia, São Paulo, BrazilWeb of Science999-1004engFundaco Oswaldo CruzMemorias Do Instituto Oswaldo CruzMycobacterium tuberculosisepitopesIFN-gamma ELISPOTlatent tuberculosisMantoux tuberculin skin testMycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin testinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/384862022-11-04 14:18:46.741metadata only accessoai:repositorio.unifesp.br:11600/38486Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-04T17:18:46Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test |
title |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test |
spellingShingle |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test Maciel da Silva, Bosco Christiano [UNIFESP] Mycobacterium tuberculosis epitopes IFN-gamma ELISPOT latent tuberculosis Mantoux tuberculin skin test |
title_short |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test |
title_full |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test |
title_fullStr |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test |
title_full_unstemmed |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test |
title_sort |
Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test |
author |
Maciel da Silva, Bosco Christiano [UNIFESP] |
author_facet |
Maciel da Silva, Bosco Christiano [UNIFESP] Rios Grassi, Maria Fernanda Coutinho, Raimundo Moreira Mascarenhas, Rita Elizabeth Olavarria, Viviana Nilla Coutinho-Borgo, Adriana [UNIFESP] Kalil, Jorge [UNIFESP] Cunha-Neto, Edecio [UNIFESP] Fonseca, Simone Goncalves [UNIFESP] |
author_role |
author |
author2 |
Rios Grassi, Maria Fernanda Coutinho, Raimundo Moreira Mascarenhas, Rita Elizabeth Olavarria, Viviana Nilla Coutinho-Borgo, Adriana [UNIFESP] Kalil, Jorge [UNIFESP] Cunha-Neto, Edecio [UNIFESP] Fonseca, Simone Goncalves [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fundacao Oswaldo Cruz Escola Bahiana Med & Saude Publ Inst Invest Immunol |
dc.contributor.author.fl_str_mv |
Maciel da Silva, Bosco Christiano [UNIFESP] Rios Grassi, Maria Fernanda Coutinho, Raimundo Moreira Mascarenhas, Rita Elizabeth Olavarria, Viviana Nilla Coutinho-Borgo, Adriana [UNIFESP] Kalil, Jorge [UNIFESP] Cunha-Neto, Edecio [UNIFESP] Fonseca, Simone Goncalves [UNIFESP] |
dc.subject.eng.fl_str_mv |
Mycobacterium tuberculosis epitopes IFN-gamma ELISPOT latent tuberculosis Mantoux tuberculin skin test |
topic |
Mycobacterium tuberculosis epitopes IFN-gamma ELISPOT latent tuberculosis Mantoux tuberculin skin test |
description |
The interferon (IFN)-gamma response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4(+) T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-gamma enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-gamma production by PBMCs from at least 31% of the TST-positive donors. the magnitude of the response against all peptides was 182 +/- 230 x 10(6) IFN-gamma spot forming cells (SFC) among TST-positive donors and 36 +/- 62 x 10(6) SFC among TST-negative donors (p = 0.007). the response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-gamma assays to identify individuals with this condition. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-12-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:13Z |
dc.date.available.fl_str_mv |
2016-01-24T14:38:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Memorias Do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 109, n. 8, p. 999-1004, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38486 http://dx.doi.org/10.1590/0074-0276140193 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
dc.identifier.file.none.fl_str_mv |
S0074-02762014000700970.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0074-02762014000700970 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0074-0276140193 |
dc.identifier.wos.none.fl_str_mv |
WOS:000347528000005 |
identifier_str_mv |
Memorias Do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 109, n. 8, p. 999-1004, 2014. 0074-0276 S0074-02762014000700970.pdf S0074-02762014000700970 10.1590/0074-0276140193 WOS:000347528000005 |
url |
http://repositorio.unifesp.br/handle/11600/38486 http://dx.doi.org/10.1590/0074-0276140193 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Memorias Do Instituto Oswaldo Cruz |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
999-1004 |
dc.publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764183375183872 |