Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/00130000161fx |
DOI: | 10.1590/S0103-50532012005000036 |
Texto Completo: | http://dx.doi.org/10.1590/S0103-50532012005000036 http://repositorio.unifesp.br/handle/11600/7259 |
Resumo: | In mass spectrometric (MS) systems with electrospray ionization (ESI), the sample can be analyzed coupled to separation systems (such as liquid chromatography or capillary electrophoresis) or simply by direct infusion. The greatest benefit of the type of injection is the possibility of continuous use of small amounts of samples over a long period of time. This extended analysis time allows a complete study of fragmentation by mass spectrometry, which is critical for structure elucidation of new compounds, or when using an ion trap mass analyzer. The injector filled with the sample is placed at the ESI source inlet creating an electric field suitable for the continuous formation of a spray (solvent and sample) and consequently, the gradual and even release of the sample. For the formation of the spray, is necessary that the injector end is metalized. The formation of a bilayer of titanium and gold provided an excellent attachment of the film, resulting in a nanoinjector for ionization/spray formation in the system for MS. The nanoinjectors showed high repeatability and stability over 100 min by continuous sampling with 10 µL of sample. |
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Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)mass spectrometrynanoinjectormicrosystemsIn mass spectrometric (MS) systems with electrospray ionization (ESI), the sample can be analyzed coupled to separation systems (such as liquid chromatography or capillary electrophoresis) or simply by direct infusion. The greatest benefit of the type of injection is the possibility of continuous use of small amounts of samples over a long period of time. This extended analysis time allows a complete study of fragmentation by mass spectrometry, which is critical for structure elucidation of new compounds, or when using an ion trap mass analyzer. The injector filled with the sample is placed at the ESI source inlet creating an electric field suitable for the continuous formation of a spray (solvent and sample) and consequently, the gradual and even release of the sample. For the formation of the spray, is necessary that the injector end is metalized. The formation of a bilayer of titanium and gold provided an excellent attachment of the film, resulting in a nanoinjector for ionization/spray formation in the system for MS. The nanoinjectors showed high repeatability and stability over 100 min by continuous sampling with 10 µL of sample.Em espectrometria de massas (MS) no modo de ionização eletrospray (ESI), as amostras podem ser analisadas com um método prévio de separação (cromatografia líquida ou eletroforese capilar) ou por infusão direta da amostra. A injeção direta apresenta um grande benefício que é a redução do volume de amostra consumido e a possibilidade de amostragem contínua por um período estendido. Este maior tempo de amostragem possibilita a análise completa através de fragmentações sucessivas por espectrometria de massas, crítico quando se busca elucidação estrutural de novos compostos, ou quando se dispõe de um analisador de massas do tipo captura de íons. Neste trabalho, descrevemos uma metodologia de deposição de filme estável na extremidade cônica dos dispositivos de boro-silicato, visando o desenvolvimento de nanoinjetores estáticos. A formação de uma camada dupla de titânio e ouro proporcionou uma excelente fixação do filme, resultando em um nanoinjetor para amostragem por ionização/formação do aerossol no sistema de espectrometria de massas. O objetivo deste filme é manter o contato elétrico no sistema. Os nanoinjetores apresentaram repetibilidade e estabilidade elevadas por mais de 100 min de amostragem contínua com apenas 10 µL de amostra.Universidade Federal de São Paulo (UNIFESP) Departamento de Ciências Exatas e da TerraUniversidade de São Paulo Instituto de Ciências BiomédicasUniversidade de São Paulo Instituto de Química de São CarlosUNIFESP, Depto. de Ciências Exatas e da TerraSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira de QuímicaUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Assunção, Nilson Antonio [UNIFESP]Nakayasu, Ernesto SatoshiDaffre, SirleiCarrilho, Emanuel2015-06-14T13:44:54Z2015-06-14T13:44:54Z2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1762-1766application/pdfhttp://dx.doi.org/10.1590/S0103-50532012005000036Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 23, n. 9, p. 1762-1766, 2012.10.1590/S0103-50532012005000036S0103-50532012000900023.pdf0103-5053S0103-50532012000900023http://repositorio.unifesp.br/handle/11600/7259WOS:000310015100024ark:/48912/00130000161fxengJournal of the Brazilian Chemical Societyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T21:27:56Zoai:repositorio.unifesp.br/:11600/7259Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:58:48.810414Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) |
title |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) |
spellingShingle |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) Assunção, Nilson Antonio [UNIFESP] mass spectrometry nanoinjector microsystems Assunção, Nilson Antonio [UNIFESP] mass spectrometry nanoinjector microsystems |
title_short |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) |
title_full |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) |
title_fullStr |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) |
title_full_unstemmed |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) |
title_sort |
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn) |
author |
Assunção, Nilson Antonio [UNIFESP] |
author_facet |
Assunção, Nilson Antonio [UNIFESP] Assunção, Nilson Antonio [UNIFESP] Nakayasu, Ernesto Satoshi Daffre, Sirlei Carrilho, Emanuel Nakayasu, Ernesto Satoshi Daffre, Sirlei Carrilho, Emanuel |
author_role |
author |
author2 |
Nakayasu, Ernesto Satoshi Daffre, Sirlei Carrilho, Emanuel |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Assunção, Nilson Antonio [UNIFESP] Nakayasu, Ernesto Satoshi Daffre, Sirlei Carrilho, Emanuel |
dc.subject.por.fl_str_mv |
mass spectrometry nanoinjector microsystems |
topic |
mass spectrometry nanoinjector microsystems |
description |
In mass spectrometric (MS) systems with electrospray ionization (ESI), the sample can be analyzed coupled to separation systems (such as liquid chromatography or capillary electrophoresis) or simply by direct infusion. The greatest benefit of the type of injection is the possibility of continuous use of small amounts of samples over a long period of time. This extended analysis time allows a complete study of fragmentation by mass spectrometry, which is critical for structure elucidation of new compounds, or when using an ion trap mass analyzer. The injector filled with the sample is placed at the ESI source inlet creating an electric field suitable for the continuous formation of a spray (solvent and sample) and consequently, the gradual and even release of the sample. For the formation of the spray, is necessary that the injector end is metalized. The formation of a bilayer of titanium and gold provided an excellent attachment of the film, resulting in a nanoinjector for ionization/spray formation in the system for MS. The nanoinjectors showed high repeatability and stability over 100 min by continuous sampling with 10 µL of sample. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 2015-06-14T13:44:54Z 2015-06-14T13:44:54Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0103-50532012005000036 Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 23, n. 9, p. 1762-1766, 2012. 10.1590/S0103-50532012005000036 S0103-50532012000900023.pdf 0103-5053 S0103-50532012000900023 http://repositorio.unifesp.br/handle/11600/7259 WOS:000310015100024 |
dc.identifier.dark.fl_str_mv |
ark:/48912/00130000161fx |
url |
http://dx.doi.org/10.1590/S0103-50532012005000036 http://repositorio.unifesp.br/handle/11600/7259 |
identifier_str_mv |
Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 23, n. 9, p. 1762-1766, 2012. 10.1590/S0103-50532012005000036 S0103-50532012000900023.pdf 0103-5053 S0103-50532012000900023 WOS:000310015100024 ark:/48912/00130000161fx |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the Brazilian Chemical Society |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1762-1766 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1822251704965398528 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0103-50532012005000036 |