Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)

Detalhes bibliográficos
Autor(a) principal: Assunção, Nilson Antonio [UNIFESP]
Data de Publicação: 2012
Outros Autores: Nakayasu, Ernesto Satoshi, Daffre, Sirlei, Carrilho, Emanuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/00130000161fx
DOI: 10.1590/S0103-50532012005000036
Texto Completo: http://dx.doi.org/10.1590/S0103-50532012005000036
http://repositorio.unifesp.br/handle/11600/7259
Resumo: In mass spectrometric (MS) systems with electrospray ionization (ESI), the sample can be analyzed coupled to separation systems (such as liquid chromatography or capillary electrophoresis) or simply by direct infusion. The greatest benefit of the type of injection is the possibility of continuous use of small amounts of samples over a long period of time. This extended analysis time allows a complete study of fragmentation by mass spectrometry, which is critical for structure elucidation of new compounds, or when using an ion trap mass analyzer. The injector filled with the sample is placed at the ESI source inlet creating an electric field suitable for the continuous formation of a spray (solvent and sample) and consequently, the gradual and even release of the sample. For the formation of the spray, is necessary that the injector end is metalized. The formation of a bilayer of titanium and gold provided an excellent attachment of the film, resulting in a nanoinjector for ionization/spray formation in the system for MS. The nanoinjectors showed high repeatability and stability over 100 min by continuous sampling with 10 µL of sample.
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spelling Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)mass spectrometrynanoinjectormicrosystemsIn mass spectrometric (MS) systems with electrospray ionization (ESI), the sample can be analyzed coupled to separation systems (such as liquid chromatography or capillary electrophoresis) or simply by direct infusion. The greatest benefit of the type of injection is the possibility of continuous use of small amounts of samples over a long period of time. This extended analysis time allows a complete study of fragmentation by mass spectrometry, which is critical for structure elucidation of new compounds, or when using an ion trap mass analyzer. The injector filled with the sample is placed at the ESI source inlet creating an electric field suitable for the continuous formation of a spray (solvent and sample) and consequently, the gradual and even release of the sample. For the formation of the spray, is necessary that the injector end is metalized. The formation of a bilayer of titanium and gold provided an excellent attachment of the film, resulting in a nanoinjector for ionization/spray formation in the system for MS. The nanoinjectors showed high repeatability and stability over 100 min by continuous sampling with 10 µL of sample.Em espectrometria de massas (MS) no modo de ionização eletrospray (ESI), as amostras podem ser analisadas com um método prévio de separação (cromatografia líquida ou eletroforese capilar) ou por infusão direta da amostra. A injeção direta apresenta um grande benefício que é a redução do volume de amostra consumido e a possibilidade de amostragem contínua por um período estendido. Este maior tempo de amostragem possibilita a análise completa através de fragmentações sucessivas por espectrometria de massas, crítico quando se busca elucidação estrutural de novos compostos, ou quando se dispõe de um analisador de massas do tipo captura de íons. Neste trabalho, descrevemos uma metodologia de deposição de filme estável na extremidade cônica dos dispositivos de boro-silicato, visando o desenvolvimento de nanoinjetores estáticos. A formação de uma camada dupla de titânio e ouro proporcionou uma excelente fixação do filme, resultando em um nanoinjetor para amostragem por ionização/formação do aerossol no sistema de espectrometria de massas. O objetivo deste filme é manter o contato elétrico no sistema. Os nanoinjetores apresentaram repetibilidade e estabilidade elevadas por mais de 100 min de amostragem contínua com apenas 10 µL de amostra.Universidade Federal de São Paulo (UNIFESP) Departamento de Ciências Exatas e da TerraUniversidade de São Paulo Instituto de Ciências BiomédicasUniversidade de São Paulo Instituto de Química de São CarlosUNIFESP, Depto. de Ciências Exatas e da TerraSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira de QuímicaUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Assunção, Nilson Antonio [UNIFESP]Nakayasu, Ernesto SatoshiDaffre, SirleiCarrilho, Emanuel2015-06-14T13:44:54Z2015-06-14T13:44:54Z2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1762-1766application/pdfhttp://dx.doi.org/10.1590/S0103-50532012005000036Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 23, n. 9, p. 1762-1766, 2012.10.1590/S0103-50532012005000036S0103-50532012000900023.pdf0103-5053S0103-50532012000900023http://repositorio.unifesp.br/handle/11600/7259WOS:000310015100024ark:/48912/00130000161fxengJournal of the Brazilian Chemical Societyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T21:27:56Zoai:repositorio.unifesp.br/:11600/7259Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:58:48.810414Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
title Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
spellingShingle Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
Assunção, Nilson Antonio [UNIFESP]
mass spectrometry
nanoinjector
microsystems
Assunção, Nilson Antonio [UNIFESP]
mass spectrometry
nanoinjector
microsystems
title_short Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
title_full Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
title_fullStr Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
title_full_unstemmed Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
title_sort Development of nanoinjector devices for electrospray ionization - tandem mass spectrometry (ESI-MSn)
author Assunção, Nilson Antonio [UNIFESP]
author_facet Assunção, Nilson Antonio [UNIFESP]
Assunção, Nilson Antonio [UNIFESP]
Nakayasu, Ernesto Satoshi
Daffre, Sirlei
Carrilho, Emanuel
Nakayasu, Ernesto Satoshi
Daffre, Sirlei
Carrilho, Emanuel
author_role author
author2 Nakayasu, Ernesto Satoshi
Daffre, Sirlei
Carrilho, Emanuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Assunção, Nilson Antonio [UNIFESP]
Nakayasu, Ernesto Satoshi
Daffre, Sirlei
Carrilho, Emanuel
dc.subject.por.fl_str_mv mass spectrometry
nanoinjector
microsystems
topic mass spectrometry
nanoinjector
microsystems
description In mass spectrometric (MS) systems with electrospray ionization (ESI), the sample can be analyzed coupled to separation systems (such as liquid chromatography or capillary electrophoresis) or simply by direct infusion. The greatest benefit of the type of injection is the possibility of continuous use of small amounts of samples over a long period of time. This extended analysis time allows a complete study of fragmentation by mass spectrometry, which is critical for structure elucidation of new compounds, or when using an ion trap mass analyzer. The injector filled with the sample is placed at the ESI source inlet creating an electric field suitable for the continuous formation of a spray (solvent and sample) and consequently, the gradual and even release of the sample. For the formation of the spray, is necessary that the injector end is metalized. The formation of a bilayer of titanium and gold provided an excellent attachment of the film, resulting in a nanoinjector for ionization/spray formation in the system for MS. The nanoinjectors showed high repeatability and stability over 100 min by continuous sampling with 10 µL of sample.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
2015-06-14T13:44:54Z
2015-06-14T13:44:54Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0103-50532012005000036
Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 23, n. 9, p. 1762-1766, 2012.
10.1590/S0103-50532012005000036
S0103-50532012000900023.pdf
0103-5053
S0103-50532012000900023
http://repositorio.unifesp.br/handle/11600/7259
WOS:000310015100024
dc.identifier.dark.fl_str_mv ark:/48912/00130000161fx
url http://dx.doi.org/10.1590/S0103-50532012005000036
http://repositorio.unifesp.br/handle/11600/7259
identifier_str_mv Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 23, n. 9, p. 1762-1766, 2012.
10.1590/S0103-50532012005000036
S0103-50532012000900023.pdf
0103-5053
S0103-50532012000900023
WOS:000310015100024
ark:/48912/00130000161fx
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the Brazilian Chemical Society
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1762-1766
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1822251704965398528
dc.identifier.doi.none.fl_str_mv 10.1590/S0103-50532012005000036