High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model

Detalhes bibliográficos
Autor(a) principal: do Carmo, Luciana Simao
Data de Publicação: 2017
Outros Autores: de Oliveira, Dalila Cunha, Cortez, Mayara, Nogueira-Pedro, Amanda [UNIFESP], Paredes-Gamero, Edgar Julian [UNIFESP], Borelli, Primavera, Rogero, Marcelo Macedo, Fock, Ricardo Ambrosio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://nopr.niscair.res.in/handle/123456789/43108
https://repositorio.unifesp.br/handle/11600/53672
Resumo: Excessive intake of a high-fat diet (HFD) results in overweight, obesity and the development of insulin resistance, adipose tissue macrophage infiltration and significant increases in inflammatory biomarkers. Bone marrow mesenchymal stem cells (MSCs) are directly involved in hematopoiesis and angiogenesis, and are currently receiving considerable attention due to their remarkable applications to cell therapy. In obese people, hematopoiesis and angiogenesis can be affected as a result of the unbalanced production of several kinds of mediators, such as VEGF. VEGF production is regulated by transcription factors such as Stat-3, which can also activate other transcription factor regulators such as c-Myc, which is closely correlated to cell proliferation. Two-month-old male Wistar rats were fed an HFD and bone marrow MSCs were isolated. The cell cycle, Stat-3 and c-Myc expression, and VEGF production were evaluated. HFD animals showed greater adipose tissue mass as well as higher blood cholesterol, leptin, and C-reactive protein levels. MSCs from the HFD group showed a higher percentage of cells in the S/G2/M phase, increased production of VEGF and higher expression of c-Myc and Stat-3. These data led us to infer that HFD induces alterations in bone marrow MSCs, which could modify their modulatory capability and affect their use in cell therapies.
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spelling do Carmo, Luciana Simaode Oliveira, Dalila CunhaCortez, MayaraNogueira-Pedro, Amanda [UNIFESP]Paredes-Gamero, Edgar Julian [UNIFESP]Borelli, PrimaveraRogero, Marcelo MacedoFock, Ricardo Ambrosio2020-06-26T16:30:38Z2020-06-26T16:30:38Z2017http://nopr.niscair.res.in/handle/123456789/43108Indian Journal Of Biochemistry & Biophysics. New Delhi, v. 54, n. 43558, p. 114-120, 2017.0301-1208https://repositorio.unifesp.br/handle/11600/53672WOS000418623900003.pdfWOS:000418623900003Excessive intake of a high-fat diet (HFD) results in overweight, obesity and the development of insulin resistance, adipose tissue macrophage infiltration and significant increases in inflammatory biomarkers. Bone marrow mesenchymal stem cells (MSCs) are directly involved in hematopoiesis and angiogenesis, and are currently receiving considerable attention due to their remarkable applications to cell therapy. In obese people, hematopoiesis and angiogenesis can be affected as a result of the unbalanced production of several kinds of mediators, such as VEGF. VEGF production is regulated by transcription factors such as Stat-3, which can also activate other transcription factor regulators such as c-Myc, which is closely correlated to cell proliferation. Two-month-old male Wistar rats were fed an HFD and bone marrow MSCs were isolated. The cell cycle, Stat-3 and c-Myc expression, and VEGF production were evaluated. HFD animals showed greater adipose tissue mass as well as higher blood cholesterol, leptin, and C-reactive protein levels. MSCs from the HFD group showed a higher percentage of cells in the S/G2/M phase, increased production of VEGF and higher expression of c-Myc and Stat-3. These data led us to infer that HFD induces alterations in bone marrow MSCs, which could modify their modulatory capability and affect their use in cell therapies.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Ave Lineu Prestes 580,Bloco 17, BR-05508900 Sao Paulo, BrazilUniv Fed Sao Paulo, Paulista Sch Med, Dept Biochem, R Sena Madureira 1500, BR-04021001 Sao Paulo, BrazilUniv Sao Paulo, Sch Publ Hlth, Dept Nutr, 715 Cerqueira Cesar, BR-03178200 Sao Paulo, BrazilUniv Fed Sao Paulo, Paulista Sch Med, Dept Biochem, R Sena Madureira 1500, BR-04021001 Sao Paulo, BrazilFAPESPWeb of Science114-120engNatl Inst Science Communication-NiscairIndian Journal Of Biochemistry & BiophysicsCell cycleCell therapyCholesterolHematopoiesisObesityTranscription factorsTranscription regulatorHigh-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNew Delhi54info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000418623900003.pdfapplication/pdf750441${dspace.ui.url}/bitstream/11600/53672/1/WOS000418623900003.pdf76513fbf04118c01ad7a3cc52a5deb82MD51open accessTEXTWOS000418623900003.pdf.txtWOS000418623900003.pdf.txtExtracted texttext/plain31439${dspace.ui.url}/bitstream/11600/53672/2/WOS000418623900003.pdf.txta246450cea89a1d9cd054235e05b3f28MD52open accessTHUMBNAILWOS000418623900003.pdf.jpgWOS000418623900003.pdf.jpgIM Thumbnailimage/jpeg5893${dspace.ui.url}/bitstream/11600/53672/4/WOS000418623900003.pdf.jpg57f1810c9e58317e5fe31c7b4dbc7767MD54open access11600/536722022-08-01 09:43:36.628open accessoai:repositorio.unifesp.br:11600/53672Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:24:13.427354Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
title High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
spellingShingle High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
do Carmo, Luciana Simao
Cell cycle
Cell therapy
Cholesterol
Hematopoiesis
Obesity
Transcription factors
Transcription regulator
title_short High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
title_full High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
title_fullStr High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
title_full_unstemmed High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
title_sort High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
author do Carmo, Luciana Simao
author_facet do Carmo, Luciana Simao
de Oliveira, Dalila Cunha
Cortez, Mayara
Nogueira-Pedro, Amanda [UNIFESP]
Paredes-Gamero, Edgar Julian [UNIFESP]
Borelli, Primavera
Rogero, Marcelo Macedo
Fock, Ricardo Ambrosio
author_role author
author2 de Oliveira, Dalila Cunha
Cortez, Mayara
Nogueira-Pedro, Amanda [UNIFESP]
Paredes-Gamero, Edgar Julian [UNIFESP]
Borelli, Primavera
Rogero, Marcelo Macedo
Fock, Ricardo Ambrosio
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv do Carmo, Luciana Simao
de Oliveira, Dalila Cunha
Cortez, Mayara
Nogueira-Pedro, Amanda [UNIFESP]
Paredes-Gamero, Edgar Julian [UNIFESP]
Borelli, Primavera
Rogero, Marcelo Macedo
Fock, Ricardo Ambrosio
dc.subject.eng.fl_str_mv Cell cycle
Cell therapy
Cholesterol
Hematopoiesis
Obesity
Transcription factors
Transcription regulator
topic Cell cycle
Cell therapy
Cholesterol
Hematopoiesis
Obesity
Transcription factors
Transcription regulator
description Excessive intake of a high-fat diet (HFD) results in overweight, obesity and the development of insulin resistance, adipose tissue macrophage infiltration and significant increases in inflammatory biomarkers. Bone marrow mesenchymal stem cells (MSCs) are directly involved in hematopoiesis and angiogenesis, and are currently receiving considerable attention due to their remarkable applications to cell therapy. In obese people, hematopoiesis and angiogenesis can be affected as a result of the unbalanced production of several kinds of mediators, such as VEGF. VEGF production is regulated by transcription factors such as Stat-3, which can also activate other transcription factor regulators such as c-Myc, which is closely correlated to cell proliferation. Two-month-old male Wistar rats were fed an HFD and bone marrow MSCs were isolated. The cell cycle, Stat-3 and c-Myc expression, and VEGF production were evaluated. HFD animals showed greater adipose tissue mass as well as higher blood cholesterol, leptin, and C-reactive protein levels. MSCs from the HFD group showed a higher percentage of cells in the S/G2/M phase, increased production of VEGF and higher expression of c-Myc and Stat-3. These data led us to infer that HFD induces alterations in bone marrow MSCs, which could modify their modulatory capability and affect their use in cell therapies.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2020-06-26T16:30:38Z
dc.date.available.fl_str_mv 2020-06-26T16:30:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.].fl_str_mv http://nopr.niscair.res.in/handle/123456789/43108
dc.identifier.citation.fl_str_mv Indian Journal Of Biochemistry & Biophysics. New Delhi, v. 54, n. 43558, p. 114-120, 2017.
dc.identifier.uri.fl_str_mv https://repositorio.unifesp.br/handle/11600/53672
dc.identifier.issn.none.fl_str_mv 0301-1208
dc.identifier.file.none.fl_str_mv WOS000418623900003.pdf
dc.identifier.wos.none.fl_str_mv WOS:000418623900003
url http://nopr.niscair.res.in/handle/123456789/43108
https://repositorio.unifesp.br/handle/11600/53672
identifier_str_mv Indian Journal Of Biochemistry & Biophysics. New Delhi, v. 54, n. 43558, p. 114-120, 2017.
0301-1208
WOS000418623900003.pdf
WOS:000418623900003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Indian Journal Of Biochemistry & Biophysics
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dc.format.none.fl_str_mv 114-120
dc.coverage.none.fl_str_mv New Delhi
dc.publisher.none.fl_str_mv Natl Inst Science Communication-Niscair
publisher.none.fl_str_mv Natl Inst Science Communication-Niscair
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
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