High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://nopr.niscair.res.in/handle/123456789/43108 https://repositorio.unifesp.br/handle/11600/53672 |
Resumo: | Excessive intake of a high-fat diet (HFD) results in overweight, obesity and the development of insulin resistance, adipose tissue macrophage infiltration and significant increases in inflammatory biomarkers. Bone marrow mesenchymal stem cells (MSCs) are directly involved in hematopoiesis and angiogenesis, and are currently receiving considerable attention due to their remarkable applications to cell therapy. In obese people, hematopoiesis and angiogenesis can be affected as a result of the unbalanced production of several kinds of mediators, such as VEGF. VEGF production is regulated by transcription factors such as Stat-3, which can also activate other transcription factor regulators such as c-Myc, which is closely correlated to cell proliferation. Two-month-old male Wistar rats were fed an HFD and bone marrow MSCs were isolated. The cell cycle, Stat-3 and c-Myc expression, and VEGF production were evaluated. HFD animals showed greater adipose tissue mass as well as higher blood cholesterol, leptin, and C-reactive protein levels. MSCs from the HFD group showed a higher percentage of cells in the S/G2/M phase, increased production of VEGF and higher expression of c-Myc and Stat-3. These data led us to infer that HFD induces alterations in bone marrow MSCs, which could modify their modulatory capability and affect their use in cell therapies. |
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do Carmo, Luciana Simaode Oliveira, Dalila CunhaCortez, MayaraNogueira-Pedro, Amanda [UNIFESP]Paredes-Gamero, Edgar Julian [UNIFESP]Borelli, PrimaveraRogero, Marcelo MacedoFock, Ricardo Ambrosio2020-06-26T16:30:38Z2020-06-26T16:30:38Z2017http://nopr.niscair.res.in/handle/123456789/43108Indian Journal Of Biochemistry & Biophysics. New Delhi, v. 54, n. 43558, p. 114-120, 2017.0301-1208https://repositorio.unifesp.br/handle/11600/53672WOS000418623900003.pdfWOS:000418623900003Excessive intake of a high-fat diet (HFD) results in overweight, obesity and the development of insulin resistance, adipose tissue macrophage infiltration and significant increases in inflammatory biomarkers. Bone marrow mesenchymal stem cells (MSCs) are directly involved in hematopoiesis and angiogenesis, and are currently receiving considerable attention due to their remarkable applications to cell therapy. In obese people, hematopoiesis and angiogenesis can be affected as a result of the unbalanced production of several kinds of mediators, such as VEGF. VEGF production is regulated by transcription factors such as Stat-3, which can also activate other transcription factor regulators such as c-Myc, which is closely correlated to cell proliferation. Two-month-old male Wistar rats were fed an HFD and bone marrow MSCs were isolated. The cell cycle, Stat-3 and c-Myc expression, and VEGF production were evaluated. HFD animals showed greater adipose tissue mass as well as higher blood cholesterol, leptin, and C-reactive protein levels. MSCs from the HFD group showed a higher percentage of cells in the S/G2/M phase, increased production of VEGF and higher expression of c-Myc and Stat-3. These data led us to infer that HFD induces alterations in bone marrow MSCs, which could modify their modulatory capability and affect their use in cell therapies.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Ave Lineu Prestes 580,Bloco 17, BR-05508900 Sao Paulo, BrazilUniv Fed Sao Paulo, Paulista Sch Med, Dept Biochem, R Sena Madureira 1500, BR-04021001 Sao Paulo, BrazilUniv Sao Paulo, Sch Publ Hlth, Dept Nutr, 715 Cerqueira Cesar, BR-03178200 Sao Paulo, BrazilUniv Fed Sao Paulo, Paulista Sch Med, Dept Biochem, R Sena Madureira 1500, BR-04021001 Sao Paulo, BrazilFAPESPWeb of Science114-120engNatl Inst Science Communication-NiscairIndian Journal Of Biochemistry & BiophysicsCell cycleCell therapyCholesterolHematopoiesisObesityTranscription factorsTranscription regulatorHigh-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNew Delhi54info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000418623900003.pdfapplication/pdf750441${dspace.ui.url}/bitstream/11600/53672/1/WOS000418623900003.pdf76513fbf04118c01ad7a3cc52a5deb82MD51open accessTEXTWOS000418623900003.pdf.txtWOS000418623900003.pdf.txtExtracted texttext/plain31439${dspace.ui.url}/bitstream/11600/53672/2/WOS000418623900003.pdf.txta246450cea89a1d9cd054235e05b3f28MD52open accessTHUMBNAILWOS000418623900003.pdf.jpgWOS000418623900003.pdf.jpgIM Thumbnailimage/jpeg5893${dspace.ui.url}/bitstream/11600/53672/4/WOS000418623900003.pdf.jpg57f1810c9e58317e5fe31c7b4dbc7767MD54open access11600/536722022-08-01 09:43:36.628open accessoai:repositorio.unifesp.br:11600/53672Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T12:43:36Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model |
title |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model |
spellingShingle |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model do Carmo, Luciana Simao Cell cycle Cell therapy Cholesterol Hematopoiesis Obesity Transcription factors Transcription regulator |
title_short |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model |
title_full |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model |
title_fullStr |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model |
title_full_unstemmed |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model |
title_sort |
High-fat diet increases STAT-3 and c-Myc expression and induces VEGF production in hone marrow mesenchymal stem cells in a rat model |
author |
do Carmo, Luciana Simao |
author_facet |
do Carmo, Luciana Simao de Oliveira, Dalila Cunha Cortez, Mayara Nogueira-Pedro, Amanda [UNIFESP] Paredes-Gamero, Edgar Julian [UNIFESP] Borelli, Primavera Rogero, Marcelo Macedo Fock, Ricardo Ambrosio |
author_role |
author |
author2 |
de Oliveira, Dalila Cunha Cortez, Mayara Nogueira-Pedro, Amanda [UNIFESP] Paredes-Gamero, Edgar Julian [UNIFESP] Borelli, Primavera Rogero, Marcelo Macedo Fock, Ricardo Ambrosio |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
do Carmo, Luciana Simao de Oliveira, Dalila Cunha Cortez, Mayara Nogueira-Pedro, Amanda [UNIFESP] Paredes-Gamero, Edgar Julian [UNIFESP] Borelli, Primavera Rogero, Marcelo Macedo Fock, Ricardo Ambrosio |
dc.subject.eng.fl_str_mv |
Cell cycle Cell therapy Cholesterol Hematopoiesis Obesity Transcription factors Transcription regulator |
topic |
Cell cycle Cell therapy Cholesterol Hematopoiesis Obesity Transcription factors Transcription regulator |
description |
Excessive intake of a high-fat diet (HFD) results in overweight, obesity and the development of insulin resistance, adipose tissue macrophage infiltration and significant increases in inflammatory biomarkers. Bone marrow mesenchymal stem cells (MSCs) are directly involved in hematopoiesis and angiogenesis, and are currently receiving considerable attention due to their remarkable applications to cell therapy. In obese people, hematopoiesis and angiogenesis can be affected as a result of the unbalanced production of several kinds of mediators, such as VEGF. VEGF production is regulated by transcription factors such as Stat-3, which can also activate other transcription factor regulators such as c-Myc, which is closely correlated to cell proliferation. Two-month-old male Wistar rats were fed an HFD and bone marrow MSCs were isolated. The cell cycle, Stat-3 and c-Myc expression, and VEGF production were evaluated. HFD animals showed greater adipose tissue mass as well as higher blood cholesterol, leptin, and C-reactive protein levels. MSCs from the HFD group showed a higher percentage of cells in the S/G2/M phase, increased production of VEGF and higher expression of c-Myc and Stat-3. These data led us to infer that HFD induces alterations in bone marrow MSCs, which could modify their modulatory capability and affect their use in cell therapies. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-06-26T16:30:38Z |
dc.date.available.fl_str_mv |
2020-06-26T16:30:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
status_str |
publishedVersion |
dc.identifier.].fl_str_mv |
http://nopr.niscair.res.in/handle/123456789/43108 |
dc.identifier.citation.fl_str_mv |
Indian Journal Of Biochemistry & Biophysics. New Delhi, v. 54, n. 43558, p. 114-120, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/53672 |
dc.identifier.issn.none.fl_str_mv |
0301-1208 |
dc.identifier.file.none.fl_str_mv |
WOS000418623900003.pdf |
dc.identifier.wos.none.fl_str_mv |
WOS:000418623900003 |
url |
http://nopr.niscair.res.in/handle/123456789/43108 https://repositorio.unifesp.br/handle/11600/53672 |
identifier_str_mv |
Indian Journal Of Biochemistry & Biophysics. New Delhi, v. 54, n. 43558, p. 114-120, 2017. 0301-1208 WOS000418623900003.pdf WOS:000418623900003 |
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eng |
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eng |
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Indian Journal Of Biochemistry & Biophysics |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
114-120 |
dc.coverage.none.fl_str_mv |
New Delhi |
dc.publisher.none.fl_str_mv |
Natl Inst Science Communication-Niscair |
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Natl Inst Science Communication-Niscair |
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