Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/6672 http://dx.doi.org/10.5581/1516-8484.20110100 |
Resumo: | BACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process. |
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Teixeira, Daniela [UNIFESP]Longo-Maugéri, Ieda Maria [UNIFESP]Santos, Jair Licio FerreiraDuarte, Yeda Aparecida OliveiraLebrão, Maria LuciaBueno, Valquiria [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2015-06-14T13:43:19Z2015-06-14T13:43:19Z2011-10-01Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011.1516-8484http://repositorio.unifesp.br/handle/11600/6672http://dx.doi.org/10.5581/1516-8484.20110100S1516-84842011000500013.pdfS1516-8484201100050001310.5581/1516-8484.20110100BACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo (UNIFESP) Immunology DepartmentUniversidade de São Paulo Faculdade de Medicina de Ribeirão PretoUniversidade de São Paulo Escola de Saúde PúblicaUniversidade Federal de São Paulo (UNIFESP) Biosciences DepartmentUNIFESP, Immunology DepartmentUNIFESP, Biosciences DepartmentSciELO367-371engAssociação Brasileira de Hematologia e Hemoterapia e Terapia CelularRevista Brasileira de Hematologia e HemoterapiaAgingSex distributionImmune SystemLymphocytesFlow CytometryEvaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo cityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1516-84842011000500013.pdfapplication/pdf1038663${dspace.ui.url}/bitstream/11600/6672/1/S1516-84842011000500013.pdffaa40f779aab5d2d02a9eda4cd44461cMD51open accessTEXTS1516-84842011000500013.pdf.txtS1516-84842011000500013.pdf.txtExtracted texttext/plain20466${dspace.ui.url}/bitstream/11600/6672/2/S1516-84842011000500013.pdf.txt0229fd8335c1a4f0780803bb0d39b749MD52open access11600/66722022-02-08 11:53:19.186open accessoai:repositorio.unifesp.br:11600/6672Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:26:47.702505Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
spellingShingle |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city Teixeira, Daniela [UNIFESP] Aging Sex distribution Immune System Lymphocytes Flow Cytometry |
title_short |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_full |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_fullStr |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_full_unstemmed |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
title_sort |
Evaluation of lymphocyte levels in a random sample of 218 elderly individuals from São Paulo city |
author |
Teixeira, Daniela [UNIFESP] |
author_facet |
Teixeira, Daniela [UNIFESP] Longo-Maugéri, Ieda Maria [UNIFESP] Santos, Jair Licio Ferreira Duarte, Yeda Aparecida Oliveira Lebrão, Maria Lucia Bueno, Valquiria [UNIFESP] |
author_role |
author |
author2 |
Longo-Maugéri, Ieda Maria [UNIFESP] Santos, Jair Licio Ferreira Duarte, Yeda Aparecida Oliveira Lebrão, Maria Lucia Bueno, Valquiria [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Teixeira, Daniela [UNIFESP] Longo-Maugéri, Ieda Maria [UNIFESP] Santos, Jair Licio Ferreira Duarte, Yeda Aparecida Oliveira Lebrão, Maria Lucia Bueno, Valquiria [UNIFESP] |
dc.subject.eng.fl_str_mv |
Aging Sex distribution Immune System Lymphocytes Flow Cytometry |
topic |
Aging Sex distribution Immune System Lymphocytes Flow Cytometry |
description |
BACKGROUND: Age-associated changes in the immune system cause decreased protection after vaccination and increased rates of infections and tumor development. METHODS: Lymphocyte percentages were compared by gender and age to establish differences between subtypes. Three mL blood samples were obtained from 218 randomly selected individuals (60-101 years old) who live in São Paulo city. Blood was lysed with Tris phosphate buffer and stained for 30 minutes with monoclonal antibodies (CD3PerCP, CD4FITC, CD8Pe, CD19Pe) for analysis by flow cytometry. Statistical analysis was by ANOVA. RESULTS: The percentage of CD4+ T cells (p-value = 0.005) and the CD4/CD8 ratio (p-value = 0.010) were lower in men, whereas the percentage of CD8+ T cells was lower (p-value = 0.002) in women; the percentage of B cells (CD19+ ) was similar between groups. Individuals grouped by gender and age range and compared showed a drop in CD4+ cells in 75 to 79-year-old men (female: 46.1% ± 8.1% and male: 38.8% ± 10.5%; p-value = 0.023). Also, the 80 to 84-year-old group of men had a higher percentage of CD8+ (female: 20.8% ± 8.2%, and male: 27.2% ± 8.2%; p-value = 0.032). Low percentages of B cells were detected in men in the 75 to 79-year-old (p-value = 0.003), 85 to 89-year-old (p-value = 0.020) and older than 90 year old (p-value = 0.002) age ranges. CONCLUSION: Elderly men present with more changes in lymphocyte subsets compared to elderly women. These findings could demonstrate impairment in the immune response since the lower CD4+ in men would provide less help to B cells (also lower in men) in terms of antibody production. In addition, the increase in CD8+ cells in this group could represent chronic inflammation observed during the aging process. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-10-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:43:19Z |
dc.date.available.fl_str_mv |
2015-06-14T13:43:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/6672 http://dx.doi.org/10.5581/1516-8484.20110100 |
dc.identifier.issn.none.fl_str_mv |
1516-8484 |
dc.identifier.file.none.fl_str_mv |
S1516-84842011000500013.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1516-84842011000500013 |
dc.identifier.doi.none.fl_str_mv |
10.5581/1516-8484.20110100 |
identifier_str_mv |
Revista Brasileira de Hematologia e Hemoterapia. Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular, v. 33, n. 5, p. 367-371, 2011. 1516-8484 S1516-84842011000500013.pdf S1516-84842011000500013 10.5581/1516-8484.20110100 |
url |
http://repositorio.unifesp.br/handle/11600/6672 http://dx.doi.org/10.5581/1516-8484.20110100 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Revista Brasileira de Hematologia e Hemoterapia |
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info:eu-repo/semantics/openAccess |
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openAccess |
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367-371 |
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Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
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Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
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