Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Thais Pandini
Data de Publicação: 2016
Outros Autores: de Lucas, Rosymar Coutinho, Cazzaniga, Rodrigo Anselmo, Franca, Carolina Nunes [UNIFESP], Segato, Fernando, Taglialegna, Rafael, Maffei, Cláudia Maria Leite
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1678-9946201658069
http://repositorio.unifesp.br/handle/11600/49254
Resumo: Cryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were compared
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spelling Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazilCryptococcus NeoformansSerotypingGenotypingFingerprintingAntifungal AgentsMating TypeHiv-PatientsPolymerase-Chain-ReactionSpecies ComplexMating-TypesSerotype-AMolecular CharacterizationVar. GattiiStrainsVirulenceDnaPathogenesisCryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were comparedbesides, the studied strains did not present a significant increase in resistance to antifungal agents. In addition, the homogeneity of the molecular types and detection of the mating types suggested a low possibility of crossing among the strains.Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Clínica Médica. 14049-900 Ribeirão Preto, SP, BrazilUniversidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Bioquímica. 14049-900 Ribeirão Preto, SP, BrazilUniversidade de São Paulo, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Departamento de Biologia. 14040-901 Ribeirão Preto, SP, BrazilUniversidade Federal de Sergipe, Departamento de Morfologia. 49100-000, SE, BrazilUniversidade de Santo Amaro, UNISA, Pós Graduação em Ciências da Saúde, São Paulo, SP, BrazilUniversidade Federal de São Paulo, UNIFESP, Departamento de Medicina, São Paulo, SP, BrazilEscola de Engenharia de Lorena, Departamento de Biotecnologia. 12602-810, Lorena, SP,Universidade de São Paulo BrazilUniversidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Biologia Celular, Molecular e Bioagentes Patogênicos. 14049-900 Ribeirão Preto, SP, BrazilUniversidade Federal de Ouro Preto, Núcleo de Inovação Tecnológica, 35400-000 Ouro Preto, MG, BrazilUniversidade Federal de São Paulo, UNIFESP, Departamento de Medicina, São Paulo, SP, Brazil.Web of ScienceCoordination for the Improvement of Higher Education Personnel, Brazil - (CAPES - Institutional Quota)Wiley2019-01-21T10:29:31Z2019-01-21T10:29:31Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionUNSP 69http://dx.doi.org/10.1590/S1678-9946201658069Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao paulo, v. 58, p. UNSP 69, 2016.10.1590/S1678-9946201658069S0036-46652016005000253.pdf0036-4665S0036-46652016005000253http://repositorio.unifesp.br/handle/11600/49254WOS:000396241000020engRevista Do Instituto De Medicina Tropical De Sao Pauloinfo:eu-repo/semantics/openAccessFigueiredo, Thais Pandinide Lucas, Rosymar CoutinhoCazzaniga, Rodrigo AnselmoFranca, Carolina Nunes [UNIFESP]Segato, FernandoTaglialegna, RafaelMaffei, Cláudia Maria Leitereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-10-05T22:12:43Zoai:repositorio.unifesp.br/:11600/49254Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-10-05T22:12:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
title Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
spellingShingle Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
Figueiredo, Thais Pandini
Cryptococcus Neoformans
Serotyping
Genotyping
Fingerprinting
Antifungal Agents
Mating Type
Hiv-PatientsPolymerase-Chain-Reaction
Species Complex
Mating-Types
Serotype-A
Molecular Characterization
Var. Gattii
Strains
Virulence
Dna
Pathogenesis
title_short Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
title_full Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
title_fullStr Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
title_full_unstemmed Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
title_sort Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil
author Figueiredo, Thais Pandini
author_facet Figueiredo, Thais Pandini
de Lucas, Rosymar Coutinho
Cazzaniga, Rodrigo Anselmo
Franca, Carolina Nunes [UNIFESP]
Segato, Fernando
Taglialegna, Rafael
Maffei, Cláudia Maria Leite
author_role author
author2 de Lucas, Rosymar Coutinho
Cazzaniga, Rodrigo Anselmo
Franca, Carolina Nunes [UNIFESP]
Segato, Fernando
Taglialegna, Rafael
Maffei, Cláudia Maria Leite
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Figueiredo, Thais Pandini
de Lucas, Rosymar Coutinho
Cazzaniga, Rodrigo Anselmo
Franca, Carolina Nunes [UNIFESP]
Segato, Fernando
Taglialegna, Rafael
Maffei, Cláudia Maria Leite
dc.subject.por.fl_str_mv Cryptococcus Neoformans
Serotyping
Genotyping
Fingerprinting
Antifungal Agents
Mating Type
Hiv-PatientsPolymerase-Chain-Reaction
Species Complex
Mating-Types
Serotype-A
Molecular Characterization
Var. Gattii
Strains
Virulence
Dna
Pathogenesis
topic Cryptococcus Neoformans
Serotyping
Genotyping
Fingerprinting
Antifungal Agents
Mating Type
Hiv-PatientsPolymerase-Chain-Reaction
Species Complex
Mating-Types
Serotype-A
Molecular Characterization
Var. Gattii
Strains
Virulence
Dna
Pathogenesis
description Cryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were compared
publishDate 2016
dc.date.none.fl_str_mv 2016
2019-01-21T10:29:31Z
2019-01-21T10:29:31Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-9946201658069
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao paulo, v. 58, p. UNSP 69, 2016.
10.1590/S1678-9946201658069
S0036-46652016005000253.pdf
0036-4665
S0036-46652016005000253
http://repositorio.unifesp.br/handle/11600/49254
WOS:000396241000020
url http://dx.doi.org/10.1590/S1678-9946201658069
http://repositorio.unifesp.br/handle/11600/49254
identifier_str_mv Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao paulo, v. 58, p. UNSP 69, 2016.
10.1590/S1678-9946201658069
S0036-46652016005000253.pdf
0036-4665
S0036-46652016005000253
WOS:000396241000020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Do Instituto De Medicina Tropical De Sao Paulo
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv UNSP 69
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268370784092160

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