Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/30864 http://dx.doi.org/10.1111/j.1365-2818.2008.02049.x |
Resumo: | CD133 antigen is an integral membrane glycoprotein that can bind with different cells. Originally, however. this cellular surface antigen was expressed in human stem cells and in various cellular progenitors of the haematopoietic system. Human cord blood has been described as an excellent source of CD133(+) haematopoietic progenitor cells with a large application potential. One of the main objectives of the present study is to describe for the first time the ultrastructural characteristics of CD133(+) stem cells using transmission electronic microscopy. Another objective of the manuscript is to demonstrate through transmission electronic microscopy the molecular image of magnetic nanoparticles connected to the stein cells of great biotechnological importance, as well as demonstrating the value of this finding for electronic paramagnetic resonance and its related nanobioscientific value. Ultrastructural results showed the monoclonal antibody anti-CD133 bound to the superparamagnetic nanoparticles by the presence of electrondense granules in cell membrane, as well as in the cytoplasm, revealing the ultrastructural characteristics of CD133(+) cells, exhibiting a round morphology with discrete cytoplasmic projections, having an active nucleus that follows this morphology. the cellular cytoplasm was filled up with mitochondrias, as well as microtubules and vesicles pinocitic. characterizing the process as being related to internalization of the magnetic nanoparticles that were endocyted by the cells in question. Electronic paramagnetic resonance analysis of the CD133(+) stem cells detected that the small (spectrum) generated by the labelled cells comes from the superparamagnetic nanoparticles that are bound to them. These results strongly suggest that these CD133(+) cells can be used in nanobiotechnology applications, with benefits in different biomedical areas. |
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Pavon, L. F.Gamarra, L. F.Marti, L. C.Amaro Junior, E.Moreira-Filho, C. A.Camargo-Mathias, M. I.Okamoto, Oswaldo Keith [UNIFESP]Albert Einstein Res & Educ Inst IEPAEUniversidade de São Paulo (USP)UNESPUniversidade Federal de São Paulo (UNIFESP)2016-01-24T13:51:38Z2016-01-24T13:51:38Z2008-09-01Journal of Microscopy-oxford. Oxford: Blackwell Publishing, v. 231, n. 3, p. 374-383, 2008.0022-2720http://repositorio.unifesp.br/handle/11600/30864http://dx.doi.org/10.1111/j.1365-2818.2008.02049.x10.1111/j.1365-2818.2008.02049.xWOS:000259611000003CD133 antigen is an integral membrane glycoprotein that can bind with different cells. Originally, however. this cellular surface antigen was expressed in human stem cells and in various cellular progenitors of the haematopoietic system. Human cord blood has been described as an excellent source of CD133(+) haematopoietic progenitor cells with a large application potential. One of the main objectives of the present study is to describe for the first time the ultrastructural characteristics of CD133(+) stem cells using transmission electronic microscopy. Another objective of the manuscript is to demonstrate through transmission electronic microscopy the molecular image of magnetic nanoparticles connected to the stein cells of great biotechnological importance, as well as demonstrating the value of this finding for electronic paramagnetic resonance and its related nanobioscientific value. Ultrastructural results showed the monoclonal antibody anti-CD133 bound to the superparamagnetic nanoparticles by the presence of electrondense granules in cell membrane, as well as in the cytoplasm, revealing the ultrastructural characteristics of CD133(+) cells, exhibiting a round morphology with discrete cytoplasmic projections, having an active nucleus that follows this morphology. the cellular cytoplasm was filled up with mitochondrias, as well as microtubules and vesicles pinocitic. characterizing the process as being related to internalization of the magnetic nanoparticles that were endocyted by the cells in question. Electronic paramagnetic resonance analysis of the CD133(+) stem cells detected that the small (spectrum) generated by the labelled cells comes from the superparamagnetic nanoparticles that are bound to them. These results strongly suggest that these CD133(+) cells can be used in nanobiotechnology applications, with benefits in different biomedical areas.Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein SBIBHAEFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Albert Einstein Res & Educ Inst IEPAE, São Paulo, BrazilUniv São Paulo, Biotechnol Program, IPT, Butanan Inst, São Paulo, BrazilUniv São Paulo, Dept Radiol, São Paulo, BrazilUniv São Paulo, Dept Immunol, Inst Biomed Sci, São Paulo, BrazilUNESP, Biosci Inst, Dept Biol, Rio Claro, SP, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, BrazilSociedade Beneficente Israelita Brasileira Hospital Albert Einstein SBIBHAE: (IIEP-AE 278-07FAPESP: 02/01826-5Web of Science374-383engBlackwell PublishingJournal of Microscopy-oxfordmagnetic nanoparticlesnanobiotechnologystem cellsultrastructureUltrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applicationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/308642022-06-02 09:34:58.094metadata only accessoai:repositorio.unifesp.br:11600/30864Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:34:58Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications |
title |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications |
spellingShingle |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications Pavon, L. F. magnetic nanoparticles nanobiotechnology stem cells ultrastructure |
title_short |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications |
title_full |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications |
title_fullStr |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications |
title_full_unstemmed |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications |
title_sort |
Ultrastructural characterization of CD133(+) stem cells bound to superparamagnetic nanoparticles: possible biotechnological applications |
author |
Pavon, L. F. |
author_facet |
Pavon, L. F. Gamarra, L. F. Marti, L. C. Amaro Junior, E. Moreira-Filho, C. A. Camargo-Mathias, M. I. Okamoto, Oswaldo Keith [UNIFESP] |
author_role |
author |
author2 |
Gamarra, L. F. Marti, L. C. Amaro Junior, E. Moreira-Filho, C. A. Camargo-Mathias, M. I. Okamoto, Oswaldo Keith [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Albert Einstein Res & Educ Inst IEPAE Universidade de São Paulo (USP) UNESP Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Pavon, L. F. Gamarra, L. F. Marti, L. C. Amaro Junior, E. Moreira-Filho, C. A. Camargo-Mathias, M. I. Okamoto, Oswaldo Keith [UNIFESP] |
dc.subject.eng.fl_str_mv |
magnetic nanoparticles nanobiotechnology stem cells ultrastructure |
topic |
magnetic nanoparticles nanobiotechnology stem cells ultrastructure |
description |
CD133 antigen is an integral membrane glycoprotein that can bind with different cells. Originally, however. this cellular surface antigen was expressed in human stem cells and in various cellular progenitors of the haematopoietic system. Human cord blood has been described as an excellent source of CD133(+) haematopoietic progenitor cells with a large application potential. One of the main objectives of the present study is to describe for the first time the ultrastructural characteristics of CD133(+) stem cells using transmission electronic microscopy. Another objective of the manuscript is to demonstrate through transmission electronic microscopy the molecular image of magnetic nanoparticles connected to the stein cells of great biotechnological importance, as well as demonstrating the value of this finding for electronic paramagnetic resonance and its related nanobioscientific value. Ultrastructural results showed the monoclonal antibody anti-CD133 bound to the superparamagnetic nanoparticles by the presence of electrondense granules in cell membrane, as well as in the cytoplasm, revealing the ultrastructural characteristics of CD133(+) cells, exhibiting a round morphology with discrete cytoplasmic projections, having an active nucleus that follows this morphology. the cellular cytoplasm was filled up with mitochondrias, as well as microtubules and vesicles pinocitic. characterizing the process as being related to internalization of the magnetic nanoparticles that were endocyted by the cells in question. Electronic paramagnetic resonance analysis of the CD133(+) stem cells detected that the small (spectrum) generated by the labelled cells comes from the superparamagnetic nanoparticles that are bound to them. These results strongly suggest that these CD133(+) cells can be used in nanobiotechnology applications, with benefits in different biomedical areas. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-09-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:51:38Z |
dc.date.available.fl_str_mv |
2016-01-24T13:51:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Microscopy-oxford. Oxford: Blackwell Publishing, v. 231, n. 3, p. 374-383, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/30864 http://dx.doi.org/10.1111/j.1365-2818.2008.02049.x |
dc.identifier.issn.none.fl_str_mv |
0022-2720 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1365-2818.2008.02049.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000259611000003 |
identifier_str_mv |
Journal of Microscopy-oxford. Oxford: Blackwell Publishing, v. 231, n. 3, p. 374-383, 2008. 0022-2720 10.1111/j.1365-2818.2008.02049.x WOS:000259611000003 |
url |
http://repositorio.unifesp.br/handle/11600/30864 http://dx.doi.org/10.1111/j.1365-2818.2008.02049.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Microscopy-oxford |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
374-383 |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764138498228224 |