Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/36344 http://dx.doi.org/10.1371/journal.pone.0063372 |
Resumo: | Background: the fungal cell wall is a complex and dynamic outer structure. in pathogenic fungi its components interact with the host, determining the infection fate. the present work aimed to characterize cell wall lipids from P. brasiliensis grown in the presence and absence of human plasma. We compared the results from isolates Pb3 and Pb18, which represent different phylogenetic species that evoke distinct patterns of experimental paracoccidioidomycosis.Methodology/Principal Findings: We comparatively characterized cell wall phospholipids, fatty acids, sterols, and neutral glycolipids by using both electrospray ionization- and gas chromatography-mass spectrometry analyses of lipids extracted with organic solvents followed by fractionation in silica-gel-60. We detected 49 phospholipid species in Pb3 and 38 in Pb18, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and phosphatidic acid. in both Pb3 and Pb18, PC and PE had the most numerous species. Among the fatty acids, C18:1 and C18:2 were the most abundant species in both isolates, although C18:2 was more abundant in Pb18. There was a different effect of plasma supplementation on fatty acids depending on the fungal isolate. the prevalent glycolipid species was HexC18:0-OH/d19:2-Cer, although other four minor species were also detected. the most abundant sterol in all samples was brassicasterol. Distinct profiles of cell wall and total yeast sterols suggested that the preparations were enriched for cell wall components. the presence of plasma in the culture medium specially increased cell wall brassicasterol abundance and also other lipids.Conclusions/Significance: We here report an original comparative lipidomic analysis of P. brasiliensis cell wall. Our results open doors to understanding the role of cell wall lipids in fungal biology, and interaction with anti-fungal drugs and the host. |
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Longo, Larissa V. G. [UNIFESP]Nakayasu, Ernesto S.Gazos-Lopes, FelipeVallejo, Milene C. [UNIFESP]Matsuo, Alisson Leonardo [UNIFESP]Almeida, Igor C.Puccia, Rosana [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ Texas El Paso2016-01-24T14:31:46Z2016-01-24T14:31:46Z2013-05-17Plos One. San Francisco: Public Library Science, v. 8, n. 5, 12 p., 2013.1932-6203http://repositorio.unifesp.br/handle/11600/36344http://dx.doi.org/10.1371/journal.pone.0063372WOS000319107900039.pdf10.1371/journal.pone.0063372WOS:000319107900039Background: the fungal cell wall is a complex and dynamic outer structure. in pathogenic fungi its components interact with the host, determining the infection fate. the present work aimed to characterize cell wall lipids from P. brasiliensis grown in the presence and absence of human plasma. We compared the results from isolates Pb3 and Pb18, which represent different phylogenetic species that evoke distinct patterns of experimental paracoccidioidomycosis.Methodology/Principal Findings: We comparatively characterized cell wall phospholipids, fatty acids, sterols, and neutral glycolipids by using both electrospray ionization- and gas chromatography-mass spectrometry analyses of lipids extracted with organic solvents followed by fractionation in silica-gel-60. We detected 49 phospholipid species in Pb3 and 38 in Pb18, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and phosphatidic acid. in both Pb3 and Pb18, PC and PE had the most numerous species. Among the fatty acids, C18:1 and C18:2 were the most abundant species in both isolates, although C18:2 was more abundant in Pb18. There was a different effect of plasma supplementation on fatty acids depending on the fungal isolate. the prevalent glycolipid species was HexC18:0-OH/d19:2-Cer, although other four minor species were also detected. the most abundant sterol in all samples was brassicasterol. Distinct profiles of cell wall and total yeast sterols suggested that the preparations were enriched for cell wall components. the presence of plasma in the culture medium specially increased cell wall brassicasterol abundance and also other lipids.Conclusions/Significance: We here report an original comparative lipidomic analysis of P. brasiliensis cell wall. Our results open doors to understanding the role of cell wall lipids in fungal biology, and interaction with anti-fungal drugs and the host.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)NIHUniversidade Federal de São Paulo, UNIFESP, EPM, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniv Texas El Paso, Dept Biol Sci, Border Biomed Res Ctr, El Paso, TX 79968 USAUniversidade Federal de São Paulo, UNIFESP, EPM, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilNIH: G12MD007592NIH: 5G12RR008124-16A1NIH: 5G12RR008124-16A1S1Web of Science12engPublic Library SciencePlos OneCharacterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasmainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000319107900039.pdfapplication/pdf1382203${dspace.ui.url}/bitstream/11600/36344/1/WOS000319107900039.pdfb272bff2f821c7e071d83ce9c76d130aMD51open accessTEXTWOS000319107900039.pdf.txtWOS000319107900039.pdf.txtExtracted texttext/plain54386${dspace.ui.url}/bitstream/11600/36344/2/WOS000319107900039.pdf.txtfffe6091884f43dee23c68a9a113493bMD52open access11600/363442022-02-08 11:52:12.195open accessoai:repositorio.unifesp.br:11600/36344Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T14:52:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma |
title |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma |
spellingShingle |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma Longo, Larissa V. G. [UNIFESP] |
title_short |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma |
title_full |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma |
title_fullStr |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma |
title_full_unstemmed |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma |
title_sort |
Characterization of Cell Wall Lipids from the Pathogenic Phase of Paracoccidioides brasiliensis Cultivated in the Presence or Absence of Human Plasma |
author |
Longo, Larissa V. G. [UNIFESP] |
author_facet |
Longo, Larissa V. G. [UNIFESP] Nakayasu, Ernesto S. Gazos-Lopes, Felipe Vallejo, Milene C. [UNIFESP] Matsuo, Alisson Leonardo [UNIFESP] Almeida, Igor C. Puccia, Rosana [UNIFESP] |
author_role |
author |
author2 |
Nakayasu, Ernesto S. Gazos-Lopes, Felipe Vallejo, Milene C. [UNIFESP] Matsuo, Alisson Leonardo [UNIFESP] Almeida, Igor C. Puccia, Rosana [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Texas El Paso |
dc.contributor.author.fl_str_mv |
Longo, Larissa V. G. [UNIFESP] Nakayasu, Ernesto S. Gazos-Lopes, Felipe Vallejo, Milene C. [UNIFESP] Matsuo, Alisson Leonardo [UNIFESP] Almeida, Igor C. Puccia, Rosana [UNIFESP] |
description |
Background: the fungal cell wall is a complex and dynamic outer structure. in pathogenic fungi its components interact with the host, determining the infection fate. the present work aimed to characterize cell wall lipids from P. brasiliensis grown in the presence and absence of human plasma. We compared the results from isolates Pb3 and Pb18, which represent different phylogenetic species that evoke distinct patterns of experimental paracoccidioidomycosis.Methodology/Principal Findings: We comparatively characterized cell wall phospholipids, fatty acids, sterols, and neutral glycolipids by using both electrospray ionization- and gas chromatography-mass spectrometry analyses of lipids extracted with organic solvents followed by fractionation in silica-gel-60. We detected 49 phospholipid species in Pb3 and 38 in Pb18, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and phosphatidic acid. in both Pb3 and Pb18, PC and PE had the most numerous species. Among the fatty acids, C18:1 and C18:2 were the most abundant species in both isolates, although C18:2 was more abundant in Pb18. There was a different effect of plasma supplementation on fatty acids depending on the fungal isolate. the prevalent glycolipid species was HexC18:0-OH/d19:2-Cer, although other four minor species were also detected. the most abundant sterol in all samples was brassicasterol. Distinct profiles of cell wall and total yeast sterols suggested that the preparations were enriched for cell wall components. the presence of plasma in the culture medium specially increased cell wall brassicasterol abundance and also other lipids.Conclusions/Significance: We here report an original comparative lipidomic analysis of P. brasiliensis cell wall. Our results open doors to understanding the role of cell wall lipids in fungal biology, and interaction with anti-fungal drugs and the host. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-05-17 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:31:46Z |
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2016-01-24T14:31:46Z |
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article |
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dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 8, n. 5, 12 p., 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/36344 http://dx.doi.org/10.1371/journal.pone.0063372 |
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1932-6203 |
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WOS000319107900039.pdf |
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10.1371/journal.pone.0063372 |
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Plos One. San Francisco: Public Library Science, v. 8, n. 5, 12 p., 2013. 1932-6203 WOS000319107900039.pdf 10.1371/journal.pone.0063372 WOS:000319107900039 |
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