Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/51258 http://dx.doi.org/10.1016/j.bjid.2017.03.023 |
Resumo: | Human adenoviruses comprise an important group of etiologie agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.& para;& para;The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.& para;& para;Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2,3,5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.& para;& para;The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.& para;& para;The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice. (C) 2017 Sociedade Brasueira de Infectologia. Published by Elsevier Editera Ltda. |
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Paulini, Inarei [UNIFESP]Siqueira-Silva, JoselmaThomaz, LucianaRocha, LeticiaHarsi, CharlotteBellei, Nancy [UNIFESP]Granato, Celso [UNIFESP]2019-08-19T11:48:28Z2019-08-19T11:48:28Z2017Brazilian Journal Of Infectious Diseases. Rio De Janeiro, v. 21, n. 5, p. 500-506, 2017.1413-8670http://repositorio.unifesp.br/handle/11600/51258http://dx.doi.org/10.1016/j.bjid.2017.03.023WOS000414276300002.pdfS1413-8670201700050050010.1016/j.bjid.2017.03.023WOS:000414276300002Human adenoviruses comprise an important group of etiologie agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.& para;& para;The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.& para;& para;Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2,3,5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.& para;& para;The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.& para;& para;The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice. (C) 2017 Sociedade Brasueira de Infectologia. Published by Elsevier Editera Ltda.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed São Paulo, Dept Med, Lab Virol, Disciplina Infectol, São Paulo, SP, BrazilUniv Fed São Paulo, Inst Biociencias, Dept Microbiol, Lab Adenovirus, São Paulo, SP, BrazilInst Biociencias, Dept Microbiol, Lab Micol, São Paulo, SP, BrazilInst Butantan, Lab Bacteriol, São Paulo, SP, BrazilUniv Fed São Paulo, Dept Med, Lab Virol, Disciplina Infectol, São Paulo, SP, BrazilUniv Fed São Paulo, Inst Biociencias, Dept Microbiol, Lab Adenovirus, São Paulo, SP, BrazilFAPESP: 2011/50100-6Web of Science500-506engElsevier BrazilAdenovirusMonoclonal antibodiesImmunochromatographic testDevelopment of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infectionDevelopment of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000414276300002.pdfapplication/pdf807246${dspace.ui.url}/bitstream/11600/51258/1/WOS000414276300002.pdfd571cf7e8dbae61888f4c217a7860544MD51open accessTEXTWOS000414276300002.pdf.txtWOS000414276300002.pdf.txtExtracted texttext/plain32875${dspace.ui.url}/bitstream/11600/51258/2/WOS000414276300002.pdf.txt3d206c6b5f319007a0889ae49508f108MD52open accessTHUMBNAILWOS000414276300002.pdf.jpgWOS000414276300002.pdf.jpgIM Thumbnailimage/jpeg6546${dspace.ui.url}/bitstream/11600/51258/4/WOS000414276300002.pdf.jpgc8fee5e16f8fb548d53050f0df087b5fMD54open access11600/512582022-08-01 18:49:22.756open accessoai:repositorio.unifesp.br:11600/51258Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T21:49:22Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
dc.title.alternative.pt.fl_str_mv |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
title |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
spellingShingle |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection Paulini, Inarei [UNIFESP] Adenovirus Monoclonal antibodies Immunochromatographic test |
title_short |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
title_full |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
title_fullStr |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
title_full_unstemmed |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
title_sort |
Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection |
author |
Paulini, Inarei [UNIFESP] |
author_facet |
Paulini, Inarei [UNIFESP] Siqueira-Silva, Joselma Thomaz, Luciana Rocha, Leticia Harsi, Charlotte Bellei, Nancy [UNIFESP] Granato, Celso [UNIFESP] |
author_role |
author |
author2 |
Siqueira-Silva, Joselma Thomaz, Luciana Rocha, Leticia Harsi, Charlotte Bellei, Nancy [UNIFESP] Granato, Celso [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Paulini, Inarei [UNIFESP] Siqueira-Silva, Joselma Thomaz, Luciana Rocha, Leticia Harsi, Charlotte Bellei, Nancy [UNIFESP] Granato, Celso [UNIFESP] |
dc.subject.eng.fl_str_mv |
Adenovirus Monoclonal antibodies Immunochromatographic test |
topic |
Adenovirus Monoclonal antibodies Immunochromatographic test |
description |
Human adenoviruses comprise an important group of etiologie agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.& para;& para;The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.& para;& para;Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2,3,5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.& para;& para;The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.& para;& para;The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice. (C) 2017 Sociedade Brasueira de Infectologia. Published by Elsevier Editera Ltda. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2019-08-19T11:48:28Z |
dc.date.available.fl_str_mv |
2019-08-19T11:48:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal Of Infectious Diseases. Rio De Janeiro, v. 21, n. 5, p. 500-506, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/51258 http://dx.doi.org/10.1016/j.bjid.2017.03.023 |
dc.identifier.issn.none.fl_str_mv |
1413-8670 |
dc.identifier.file.none.fl_str_mv |
WOS000414276300002.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1413-86702017000500500 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bjid.2017.03.023 |
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WOS:000414276300002 |
identifier_str_mv |
Brazilian Journal Of Infectious Diseases. Rio De Janeiro, v. 21, n. 5, p. 500-506, 2017. 1413-8670 WOS000414276300002.pdf S1413-86702017000500500 10.1016/j.bjid.2017.03.023 WOS:000414276300002 |
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Elsevier Brazil |
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Elsevier Brazil |
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