Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/53817 https://dx.doi.org/10.1155/2018/6798238 |
Resumo: | Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by irreversible airflow limitation, airway inflammation and remodeling, and enlargement of alveolar spaces. COPD is in the top five leading causes of deaths worldwide and presents a high economic cost. However, there are some preventive measures to lower the risk of developing COPD. Low-level laser therapy (LLLT) is a new effective therapy, with very low cost and no side effects. So, our objective was to investigate if LLLT reduces pulmonary alterations in an experimental model of COPD. C57BL/6 mice were submitted to cigarette smoke for 75 days (2x/day). After 60 days to smoke exposure, the treated group was submitted to LLLT (diode laser, 660 nm, 30 mW, and 3 J/cm(2)) for 15 days and euthanized for morphologic and functional analysis of the lungs. Our results showed that LLLT significantly reduced the number of inflammatory cells and the proinflammatory cytokine secretion such as IL-1 beta, IL-6, and TNF-alpha in bronchoalveolar lavage fluid (BALF). We also observed that LLLT decreased collagen deposition as well as the expression of purinergic P2X7 receptor. On the other hand, LLLT increased the IL-10 release. Thus, LLLT can be pointed as a promising therapeutic approach for lung inflammatory diseases as COPD. |
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Moraes, Gabriel da CunhaVitoretti, Luana BeatrizBrito, Auriléia Aparecida deAlves, Cintia EstefanoRigonato-Oliveira, Nicole CristineDias, Alana dos SantosTeles Matos, Yves SilvaOliveira-Junior, Manoel CarneiroFranco Oliveira, Luis VicentePalma, Renata Kelly daCandeo, Larissa CarboneraFranco, Adriana Lino dos SantosHorliana, Anna Carolina Ratto TempestineGimenes, Joao Antonio JuniorAimbire, Flavio [UNIFESP]Vieira, Rodolfo de Paula [UNIFESP]Ligeiro-de-Oliveira, Ana PaulaUniversidade Federal de São Paulo (UNIFESP)2020-07-02T18:52:00Z2020-07-02T18:52:00Z2018Oxidative Medicine And Cellular Longevity. London, v. , p. -, 2018.1942-0900https://repositorio.unifesp.br/handle/11600/53817https://dx.doi.org/10.1155/2018/6798238WOS000427789900001.pdf10.1155/2018/6798238WOS:000427789900001Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by irreversible airflow limitation, airway inflammation and remodeling, and enlargement of alveolar spaces. COPD is in the top five leading causes of deaths worldwide and presents a high economic cost. However, there are some preventive measures to lower the risk of developing COPD. Low-level laser therapy (LLLT) is a new effective therapy, with very low cost and no side effects. So, our objective was to investigate if LLLT reduces pulmonary alterations in an experimental model of COPD. C57BL/6 mice were submitted to cigarette smoke for 75 days (2x/day). After 60 days to smoke exposure, the treated group was submitted to LLLT (diode laser, 660 nm, 30 mW, and 3 J/cm(2)) for 15 days and euthanized for morphologic and functional analysis of the lungs. Our results showed that LLLT significantly reduced the number of inflammatory cells and the proinflammatory cytokine secretion such as IL-1 beta, IL-6, and TNF-alpha in bronchoalveolar lavage fluid (BALF). We also observed that LLLT decreased collagen deposition as well as the expression of purinergic P2X7 receptor. On the other hand, LLLT increased the IL-10 release. Thus, LLLT can be pointed as a promising therapeutic approach for lung inflammatory diseases as COPD.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Nove Julho UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, Sao Paulo, SP, BrazilBrazilian Inst Teaching & Res Pulm & Exercise Imm, Sao Jose Dos Campos, SP, BrazilUniv Nove Julho UNINOVE, Masters Degree & PhD Program Rehabil Sci, Expt Cardioresp Physiol Lab, Sao Paulo, SP, BrazilUniv Calif San Diego UCSD Hlth Sci, Div Trauma Surg Crit Care Burns & Acute Care Surg, Dept Surg, San Diego, CA USAFed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Sao Jose Dos Campos, SP, BrazilUniv Brasil, Postgrad Program Bioengn, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Santos, SP, BrazilFed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Sao Jose Dos Campos, SP, BrazilFed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Santos, SP, BrazilFAPESP: 2012/16498-5FAPESP: 2012/15165-2FAPESP: 2015/23152-6FAPESP: 2014/14604-8FAPESP: 2015/13486-4Web of Science-engHindawi LtdOxidative Medicine And Cellular LongevityLow-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondoninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000427789900001.pdfapplication/pdf3321658${dspace.ui.url}/bitstream/11600/53817/1/WOS000427789900001.pdfa62cc54b76e8988ea98be0fa2cf0e330MD51open accessTEXTWOS000427789900001.pdf.txtWOS000427789900001.pdf.txtExtracted texttext/plain32732${dspace.ui.url}/bitstream/11600/53817/8/WOS000427789900001.pdf.txtfa0c9b846ae775288602ea2550bf3708MD58open accessTHUMBNAILWOS000427789900001.pdf.jpgWOS000427789900001.pdf.jpgIM Thumbnailimage/jpeg6430${dspace.ui.url}/bitstream/11600/53817/10/WOS000427789900001.pdf.jpgfff07c531a0377d56c7e62fbeabe3372MD510open access11600/538172023-06-05 19:09:14.798open accessoai:repositorio.unifesp.br:11600/53817Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:09:14Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
spellingShingle |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor Moraes, Gabriel da Cunha |
title_short |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_full |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_fullStr |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_full_unstemmed |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
title_sort |
Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor |
author |
Moraes, Gabriel da Cunha |
author_facet |
Moraes, Gabriel da Cunha Vitoretti, Luana Beatriz Brito, Auriléia Aparecida de Alves, Cintia Estefano Rigonato-Oliveira, Nicole Cristine Dias, Alana dos Santos Teles Matos, Yves Silva Oliveira-Junior, Manoel Carneiro Franco Oliveira, Luis Vicente Palma, Renata Kelly da Candeo, Larissa Carbonera Franco, Adriana Lino dos Santos Horliana, Anna Carolina Ratto Tempestine Gimenes, Joao Antonio Junior Aimbire, Flavio [UNIFESP] Vieira, Rodolfo de Paula [UNIFESP] Ligeiro-de-Oliveira, Ana Paula |
author_role |
author |
author2 |
Vitoretti, Luana Beatriz Brito, Auriléia Aparecida de Alves, Cintia Estefano Rigonato-Oliveira, Nicole Cristine Dias, Alana dos Santos Teles Matos, Yves Silva Oliveira-Junior, Manoel Carneiro Franco Oliveira, Luis Vicente Palma, Renata Kelly da Candeo, Larissa Carbonera Franco, Adriana Lino dos Santos Horliana, Anna Carolina Ratto Tempestine Gimenes, Joao Antonio Junior Aimbire, Flavio [UNIFESP] Vieira, Rodolfo de Paula [UNIFESP] Ligeiro-de-Oliveira, Ana Paula |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Moraes, Gabriel da Cunha Vitoretti, Luana Beatriz Brito, Auriléia Aparecida de Alves, Cintia Estefano Rigonato-Oliveira, Nicole Cristine Dias, Alana dos Santos Teles Matos, Yves Silva Oliveira-Junior, Manoel Carneiro Franco Oliveira, Luis Vicente Palma, Renata Kelly da Candeo, Larissa Carbonera Franco, Adriana Lino dos Santos Horliana, Anna Carolina Ratto Tempestine Gimenes, Joao Antonio Junior Aimbire, Flavio [UNIFESP] Vieira, Rodolfo de Paula [UNIFESP] Ligeiro-de-Oliveira, Ana Paula |
description |
Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by irreversible airflow limitation, airway inflammation and remodeling, and enlargement of alveolar spaces. COPD is in the top five leading causes of deaths worldwide and presents a high economic cost. However, there are some preventive measures to lower the risk of developing COPD. Low-level laser therapy (LLLT) is a new effective therapy, with very low cost and no side effects. So, our objective was to investigate if LLLT reduces pulmonary alterations in an experimental model of COPD. C57BL/6 mice were submitted to cigarette smoke for 75 days (2x/day). After 60 days to smoke exposure, the treated group was submitted to LLLT (diode laser, 660 nm, 30 mW, and 3 J/cm(2)) for 15 days and euthanized for morphologic and functional analysis of the lungs. Our results showed that LLLT significantly reduced the number of inflammatory cells and the proinflammatory cytokine secretion such as IL-1 beta, IL-6, and TNF-alpha in bronchoalveolar lavage fluid (BALF). We also observed that LLLT decreased collagen deposition as well as the expression of purinergic P2X7 receptor. On the other hand, LLLT increased the IL-10 release. Thus, LLLT can be pointed as a promising therapeutic approach for lung inflammatory diseases as COPD. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-07-02T18:52:00Z |
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2020-07-02T18:52:00Z |
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dc.identifier.citation.fl_str_mv |
Oxidative Medicine And Cellular Longevity. London, v. , p. -, 2018. |
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https://repositorio.unifesp.br/handle/11600/53817 https://dx.doi.org/10.1155/2018/6798238 |
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1942-0900 |
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10.1155/2018/6798238 |
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Oxidative Medicine And Cellular Longevity. London, v. , p. -, 2018. 1942-0900 WOS000427789900001.pdf 10.1155/2018/6798238 WOS:000427789900001 |
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