GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN

Detalhes bibliográficos
Autor(a) principal: Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Data de Publicação: 2016
Outros Autores: Kobayashi, Elsa Yoko [UNIFESP], de Saboya Lenzi, Luiz Guilherme [UNIFESP], Gomes dos Santos, Joao Baptista [UNIFESP], Nader, Helena Bonciani [UNIFESP], Faloppa, Flavio [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://repositorio.unifesp.br/handle/11600/57881
http://dx.doi.org/10.1590/1413-785220162402154342
Resumo: Objective: To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods: This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results: A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion: An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients.
id UFSP_8fca89f7233a6639d3e2287199cb4e7c
oai_identifier_str oai:repositorio.unifesp.br:11600/57881
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Hirai Nascimento, Priscilla Carneiro [UNIFESP]Kobayashi, Elsa Yoko [UNIFESP]de Saboya Lenzi, Luiz Guilherme [UNIFESP]Gomes dos Santos, Joao Baptista [UNIFESP]Nader, Helena Bonciani [UNIFESP]Faloppa, Flavio [UNIFESP]2020-08-21T17:00:08Z2020-08-21T17:00:08Z2016Acta Ortopedica Brasileira. Sao Paulo Sp, v. 24, n. 2, p. 98-101, 2016.1413-7852https://repositorio.unifesp.br/handle/11600/57881http://dx.doi.org/10.1590/1413-785220162402154342S1413-78522016000200098-en.pdfS1413-7852201600020009810.1590/1413-785220162402154342WOS:000372326500008Objective: To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods: This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results: A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion: An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients.Univ Fed Sao Paulo Unifesp, Escola Paulista Med, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, Escola Paulista Med, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Orthoped & Traumatol, Sao Paulo, SP, BrazilWeb of Science98-101engAtha Comunicacao & EditoraActa Ortopedica BrasileiraDupuytren contractureGlycosaminoglycansProteoglycansFasciaGLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTRENinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSao Paulo Sp242info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1413-78522016000200098-en.pdfapplication/pdf386371${dspace.ui.url}/bitstream/11600/57881/1/S1413-78522016000200098-en.pdfd2c52b084c4da36d0e37ba32f7d55f7dMD51open accessTEXTS1413-78522016000200098-en.pdf.txtS1413-78522016000200098-en.pdf.txtExtracted texttext/plain22897${dspace.ui.url}/bitstream/11600/57881/2/S1413-78522016000200098-en.pdf.txt7a9f7b4d5aa4b11f280bc9d65066148dMD52open accessTHUMBNAILS1413-78522016000200098-en.pdf.jpgS1413-78522016000200098-en.pdf.jpgIM Thumbnailimage/jpeg7385${dspace.ui.url}/bitstream/11600/57881/4/S1413-78522016000200098-en.pdf.jpg04c6127df917d63101b90dcedc7a89c9MD54open access11600/578812022-07-31 19:43:19.751open accessoai:repositorio.unifesp.br:11600/57881Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:26:35.481936Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
spellingShingle GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
title_short GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_full GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_fullStr GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_full_unstemmed GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_sort GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
author Hirai Nascimento, Priscilla Carneiro [UNIFESP]
author_facet Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Kobayashi, Elsa Yoko [UNIFESP]
de Saboya Lenzi, Luiz Guilherme [UNIFESP]
Gomes dos Santos, Joao Baptista [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Faloppa, Flavio [UNIFESP]
author_role author
author2 Kobayashi, Elsa Yoko [UNIFESP]
de Saboya Lenzi, Luiz Guilherme [UNIFESP]
Gomes dos Santos, Joao Baptista [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Faloppa, Flavio [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Hirai Nascimento, Priscilla Carneiro [UNIFESP]
Kobayashi, Elsa Yoko [UNIFESP]
de Saboya Lenzi, Luiz Guilherme [UNIFESP]
Gomes dos Santos, Joao Baptista [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Faloppa, Flavio [UNIFESP]
dc.subject.eng.fl_str_mv Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
topic Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
description Objective: To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods: This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results: A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion: An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2020-08-21T17:00:08Z
dc.date.available.fl_str_mv 2020-08-21T17:00:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Acta Ortopedica Brasileira. Sao Paulo Sp, v. 24, n. 2, p. 98-101, 2016.
dc.identifier.uri.fl_str_mv https://repositorio.unifesp.br/handle/11600/57881
http://dx.doi.org/10.1590/1413-785220162402154342
dc.identifier.issn.none.fl_str_mv 1413-7852
dc.identifier.file.none.fl_str_mv S1413-78522016000200098-en.pdf
dc.identifier.scielo.none.fl_str_mv S1413-78522016000200098
dc.identifier.doi.none.fl_str_mv 10.1590/1413-785220162402154342
dc.identifier.wos.none.fl_str_mv WOS:000372326500008
identifier_str_mv Acta Ortopedica Brasileira. Sao Paulo Sp, v. 24, n. 2, p. 98-101, 2016.
1413-7852
S1413-78522016000200098-en.pdf
S1413-78522016000200098
10.1590/1413-785220162402154342
WOS:000372326500008
url https://repositorio.unifesp.br/handle/11600/57881
http://dx.doi.org/10.1590/1413-785220162402154342
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Acta Ortopedica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 98-101
dc.coverage.none.fl_str_mv Sao Paulo Sp
dc.publisher.none.fl_str_mv Atha Comunicacao & Editora
publisher.none.fl_str_mv Atha Comunicacao & Editora
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
bitstream.url.fl_str_mv ${dspace.ui.url}/bitstream/11600/57881/1/S1413-78522016000200098-en.pdf
${dspace.ui.url}/bitstream/11600/57881/2/S1413-78522016000200098-en.pdf.txt
${dspace.ui.url}/bitstream/11600/57881/4/S1413-78522016000200098-en.pdf.jpg
bitstream.checksum.fl_str_mv d2c52b084c4da36d0e37ba32f7d55f7d
7a9f7b4d5aa4b11f280bc9d65066148d
04c6127df917d63101b90dcedc7a89c9
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
_version_ 1783460291284041728

Registros relacionados