GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN

Detalhes bibliográficos
Autor(a) principal: Nascimento,Priscilla Carneiro Hirai
Data de Publicação: 2016
Outros Autores: Kobayashi,Elsa Yoko, Lenzi,Luiz Guilherme de Saboya, Santos,João Baptista Gomes dos, Nader,Helena Bonciani, Faloppa,Flávio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Ortopédica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522016000200098
Resumo: Objective : To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods : This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results : A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion : An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients. Level of Evidence III, Case-Control Study.
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spelling GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTRENDupuytren contractureGlycosaminoglycansProteoglycansFascia Objective : To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods : This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results : A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion : An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients. Level of Evidence III, Case-Control Study.ATHA EDITORA2016-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522016000200098Acta Ortopédica Brasileira v.24 n.2 2016reponame:Acta Ortopédica Brasileira (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1590/1413-785220162402154342info:eu-repo/semantics/openAccessNascimento,Priscilla Carneiro HiraiKobayashi,Elsa YokoLenzi,Luiz Guilherme de SaboyaSantos,João Baptista Gomes dosNader,Helena BoncianiFaloppa,Flávioeng2016-06-03T00:00:00Zoai:scielo:S1413-78522016000200098Revistahttp://www.actaortopedica.com.br/https://old.scielo.br/oai/scielo-oai.php1atha@uol.com.br||actaortopedicabrasileira@uol.com.br1809-44061413-7852opendoar:2016-06-03T00:00Acta Ortopédica Brasileira (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false
dc.title.none.fl_str_mv GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
spellingShingle GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
Nascimento,Priscilla Carneiro Hirai
Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
title_short GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_full GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_fullStr GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_full_unstemmed GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
title_sort GLYCOSAMINOGLYCANS AND PROTEOGLYCANS IN PALMAR FASCIA OF PATIENTS WITH DUPUYTREN
author Nascimento,Priscilla Carneiro Hirai
author_facet Nascimento,Priscilla Carneiro Hirai
Kobayashi,Elsa Yoko
Lenzi,Luiz Guilherme de Saboya
Santos,João Baptista Gomes dos
Nader,Helena Bonciani
Faloppa,Flávio
author_role author
author2 Kobayashi,Elsa Yoko
Lenzi,Luiz Guilherme de Saboya
Santos,João Baptista Gomes dos
Nader,Helena Bonciani
Faloppa,Flávio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Nascimento,Priscilla Carneiro Hirai
Kobayashi,Elsa Yoko
Lenzi,Luiz Guilherme de Saboya
Santos,João Baptista Gomes dos
Nader,Helena Bonciani
Faloppa,Flávio
dc.subject.por.fl_str_mv Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
topic Dupuytren contracture
Glycosaminoglycans
Proteoglycans
Fascia
description Objective : To evaluate and compare the behavior of glycosaminoglycans (GAGs) in Dupuytren disease (DD). Methods : This is an experimental study with 23 patients diagnosed with DD. Tissue collected through fasciectomy with incision type Brunner or McCash were evaluated by electrophoresis for identification of GAGs. The quantification was carried out by immunofluorescence and dosage of proteins for different types of glycosaminoglycans. The results were expressed in percentage and statistically evaluated. Results : A significant increase was observed through eletrophoresis in GAGs, as compared to the control (p<0.05). Immunofluorescence of hyaluronic acid was reduced (23 times) when compared to the control (p<0.0001). Conclusion : An increase of sulfated GAGs in Dupuytren's disease, mainly dermatan sulfate, was evident from our results, as well as a pronounced decrease of hyaluronic acid in the palmar aponeurosis from the same patients. Level of Evidence III, Case-Control Study.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522016000200098
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522016000200098
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1413-785220162402154342
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ATHA EDITORA
publisher.none.fl_str_mv ATHA EDITORA
dc.source.none.fl_str_mv Acta Ortopédica Brasileira v.24 n.2 2016
reponame:Acta Ortopédica Brasileira (Online)
instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron:SBOT
instname_str Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron_str SBOT
institution SBOT
reponame_str Acta Ortopédica Brasileira (Online)
collection Acta Ortopédica Brasileira (Online)
repository.name.fl_str_mv Acta Ortopédica Brasileira (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
repository.mail.fl_str_mv 1atha@uol.com.br||actaortopedicabrasileira@uol.com.br
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