Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33394 http://dx.doi.org/10.1128/JCM.01274-10 |
Resumo: | Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium massiliense (Mycobacterium abscessus sensu lato) are closely related species that currently are identified by the sequencing of the rpoB gene. However, recent studies show that rpoB sequencing alone is insufficient to discriminate between these species, and some authors have questioned their current taxonomic classification. We studied here a large collection of M. abscessus (sensu lato) strains by partial rpoB sequencing (752 bp) and multilocus sequence analysis (MLSA). the final MLSA scheme developed was based on the partial sequences of eight housekeeping genes: argH, cya, glpK, gnd, murC, pgm, pta, and purH. the strains studied included the three type strains (M. abscessus CIP 104536T, M. massiliense CIP 108297(T), and M. bolletii CIP 108541(T)) and 120 isolates recovered between 1997 and 2007 in France, Germany, Switzerland, and Brazil. the rpoB phylogenetic tree confirmed the existence of three main clusters, each comprising the type strain of one species. However, divergence values between the M. massiliense and M. bolletii clusters all were below 3% and between the M. abscessus and M. massiliense clusters were from 2.66 to 3.59%. the tree produced using the concatenated MLSA gene sequences (4,071 bp) also showed three main clusters, each comprising the type strain of one species. the M. abscessus cluster had a bootstrap value of 100% and was mostly compact. Bootstrap values for the M. massiliense and M. bolletii branches were much lower (71 and 61%, respectively), with the M. massiliense cluster having a fuzzy aspect. Mean (range) divergence values were 2.17% (1.13 to 2.58%) between the M. abscessus and M. massiliense clusters, 2.37% (1.5 to 2.85%) between the M. abscessus and M. bolletii clusters, and 2.28% (0.86 to 2.68%) between the M. massiliense and M. bolletii clusters. Adding the rpoB sequence to the MLSA-concatenated sequence (total sequence, 4,823 bp) had little effect on the clustering of strains. We found 10/120 (8.3%) isolates for which the concatenated MLSA gene sequence and rpoB sequence were discordant (e. g., M. massiliense MLSA sequence and M. abscessus rpoB sequence), suggesting the intergroup lateral transfers of rpoB. in conclusion, our study strongly supports the recent proposal that M. abscessus, M. massiliense, and M. bolletii should constitute a single species. Our findings also indicate that there has been a horizontal transfer of rpoB sequences between these subgroups, precluding the use of rpoB sequencing alone for the accurate identification of the two proposed M. abscessus subspecies. |
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Macheras, EdouardRoux, Anne-LaureBastian, SylvaineLeao, Sylvia Cardoso [UNIFESP]Palaci, MoisesSivadon-Tardy, ValerieGutierrez, CristinaRichter, ElviraRuesch-Gerdes, SabinePfyffer, GabyBodmer, ThomasCambau, EmmanuelleGaillard, Jean-LouisHeym, BeateHop Ambroise PareHop Raymond PoincareUniv Versailles St Quentin En YvelinesGrp Hosp Pitie SalpetriereUniversidade Federal de São Paulo (UNIFESP)Univ Fed Espirito SantoFINDForschungszentrum BorstelLuzerner KantonsspitalUniv BernGrp Hosp St Louis Lariboisiere2016-01-24T14:06:06Z2016-01-24T14:06:06Z2011-02-01Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 49, n. 2, p. 491-499, 2011.0095-1137http://repositorio.unifesp.br/handle/11600/33394http://dx.doi.org/10.1128/JCM.01274-10WOS000286977500003.pdf10.1128/JCM.01274-10WOS:000286977500003Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium massiliense (Mycobacterium abscessus sensu lato) are closely related species that currently are identified by the sequencing of the rpoB gene. However, recent studies show that rpoB sequencing alone is insufficient to discriminate between these species, and some authors have questioned their current taxonomic classification. We studied here a large collection of M. abscessus (sensu lato) strains by partial rpoB sequencing (752 bp) and multilocus sequence analysis (MLSA). the final MLSA scheme developed was based on the partial sequences of eight housekeeping genes: argH, cya, glpK, gnd, murC, pgm, pta, and purH. the strains studied included the three type strains (M. abscessus CIP 104536T, M. massiliense CIP 108297(T), and M. bolletii CIP 108541(T)) and 120 isolates recovered between 1997 and 2007 in France, Germany, Switzerland, and Brazil. the rpoB phylogenetic tree confirmed the existence of three main clusters, each comprising the type strain of one species. However, divergence values between the M. massiliense and M. bolletii clusters all were below 3% and between the M. abscessus and M. massiliense clusters were from 2.66 to 3.59%. the tree produced using the concatenated MLSA gene sequences (4,071 bp) also showed three main clusters, each comprising the type strain of one species. the M. abscessus cluster had a bootstrap value of 100% and was mostly compact. Bootstrap values for the M. massiliense and M. bolletii branches were much lower (71 and 61%, respectively), with the M. massiliense cluster having a fuzzy aspect. Mean (range) divergence values were 2.17% (1.13 to 2.58%) between the M. abscessus and M. massiliense clusters, 2.37% (1.5 to 2.85%) between the M. abscessus and M. bolletii clusters, and 2.28% (0.86 to 2.68%) between the M. massiliense and M. bolletii clusters. Adding the rpoB sequence to the MLSA-concatenated sequence (total sequence, 4,823 bp) had little effect on the clustering of strains. We found 10/120 (8.3%) isolates for which the concatenated MLSA gene sequence and rpoB sequence were discordant (e. g., M. massiliense MLSA sequence and M. abscessus rpoB sequence), suggesting the intergroup lateral transfers of rpoB. in conclusion, our study strongly supports the recent proposal that M. abscessus, M. massiliense, and M. bolletii should constitute a single species. Our findings also indicate that there has been a horizontal transfer of rpoB sequences between these subgroups, precluding the use of rpoB sequencing alone for the accurate identification of the two proposed M. abscessus subspecies.Vaincre la MucoviscidoseHop Ambroise Pare, AP HP, Serv Microbiol Hyg, Boulogne, FranceHop Raymond Poincare, AP HP, Microbiol Lab, Garches, FranceUniv Versailles St Quentin En Yvelines, EA 3647, Garches, FranceGrp Hosp Pitie Salpetriere, Ctr Natl Reference Mycobacteries & Resistance Myc, Lab Bacteriol Hyg, F-75634 Paris, FranceUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniv Fed Espirito Santo, Nucleo Doencas Infecciosas, Vitoria, ES, BrazilFIND, Geneva, SwitzerlandForschungszentrum Borstel, Natl Reference Ctr Mycobacteria, Borstel, GermanyLuzerner Kantonsspital, Inst Med Mikrobiol, Zentrum LaborMed, Luzern, SwitzerlandUniv Bern, Inst Infekt Krankheiten, Bern, SwitzerlandGrp Hosp St Louis Lariboisiere, Ctr Natl Reference Mycobacteries & Resistance Myc, Lab Associe, Paris, FranceUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of Science491-499engAmer Soc MicrobiologyJournal of Clinical MicrobiologyMultilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000286977500003.pdfapplication/pdf859741${dspace.ui.url}/bitstream/11600/33394/1/WOS000286977500003.pdfd9f7d6e03f518eb75bed7fb0ba563f3dMD51open accessTEXTWOS000286977500003.pdf.txtWOS000286977500003.pdf.txtExtracted texttext/plain48011${dspace.ui.url}/bitstream/11600/33394/2/WOS000286977500003.pdf.txtf2d2b8f30da8f3d64a927a19c605e1ddMD52open access11600/333942023-01-30 22:17:47.122open accessoai:repositorio.unifesp.br:11600/33394Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-31T01:17:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains |
title |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains |
spellingShingle |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains Macheras, Edouard |
title_short |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains |
title_full |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains |
title_fullStr |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains |
title_full_unstemmed |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains |
title_sort |
Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains |
author |
Macheras, Edouard |
author_facet |
Macheras, Edouard Roux, Anne-Laure Bastian, Sylvaine Leao, Sylvia Cardoso [UNIFESP] Palaci, Moises Sivadon-Tardy, Valerie Gutierrez, Cristina Richter, Elvira Ruesch-Gerdes, Sabine Pfyffer, Gaby Bodmer, Thomas Cambau, Emmanuelle Gaillard, Jean-Louis Heym, Beate |
author_role |
author |
author2 |
Roux, Anne-Laure Bastian, Sylvaine Leao, Sylvia Cardoso [UNIFESP] Palaci, Moises Sivadon-Tardy, Valerie Gutierrez, Cristina Richter, Elvira Ruesch-Gerdes, Sabine Pfyffer, Gaby Bodmer, Thomas Cambau, Emmanuelle Gaillard, Jean-Louis Heym, Beate |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Hop Ambroise Pare Hop Raymond Poincare Univ Versailles St Quentin En Yvelines Grp Hosp Pitie Salpetriere Universidade Federal de São Paulo (UNIFESP) Univ Fed Espirito Santo FIND Forschungszentrum Borstel Luzerner Kantonsspital Univ Bern Grp Hosp St Louis Lariboisiere |
dc.contributor.author.fl_str_mv |
Macheras, Edouard Roux, Anne-Laure Bastian, Sylvaine Leao, Sylvia Cardoso [UNIFESP] Palaci, Moises Sivadon-Tardy, Valerie Gutierrez, Cristina Richter, Elvira Ruesch-Gerdes, Sabine Pfyffer, Gaby Bodmer, Thomas Cambau, Emmanuelle Gaillard, Jean-Louis Heym, Beate |
description |
Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium massiliense (Mycobacterium abscessus sensu lato) are closely related species that currently are identified by the sequencing of the rpoB gene. However, recent studies show that rpoB sequencing alone is insufficient to discriminate between these species, and some authors have questioned their current taxonomic classification. We studied here a large collection of M. abscessus (sensu lato) strains by partial rpoB sequencing (752 bp) and multilocus sequence analysis (MLSA). the final MLSA scheme developed was based on the partial sequences of eight housekeeping genes: argH, cya, glpK, gnd, murC, pgm, pta, and purH. the strains studied included the three type strains (M. abscessus CIP 104536T, M. massiliense CIP 108297(T), and M. bolletii CIP 108541(T)) and 120 isolates recovered between 1997 and 2007 in France, Germany, Switzerland, and Brazil. the rpoB phylogenetic tree confirmed the existence of three main clusters, each comprising the type strain of one species. However, divergence values between the M. massiliense and M. bolletii clusters all were below 3% and between the M. abscessus and M. massiliense clusters were from 2.66 to 3.59%. the tree produced using the concatenated MLSA gene sequences (4,071 bp) also showed three main clusters, each comprising the type strain of one species. the M. abscessus cluster had a bootstrap value of 100% and was mostly compact. Bootstrap values for the M. massiliense and M. bolletii branches were much lower (71 and 61%, respectively), with the M. massiliense cluster having a fuzzy aspect. Mean (range) divergence values were 2.17% (1.13 to 2.58%) between the M. abscessus and M. massiliense clusters, 2.37% (1.5 to 2.85%) between the M. abscessus and M. bolletii clusters, and 2.28% (0.86 to 2.68%) between the M. massiliense and M. bolletii clusters. Adding the rpoB sequence to the MLSA-concatenated sequence (total sequence, 4,823 bp) had little effect on the clustering of strains. We found 10/120 (8.3%) isolates for which the concatenated MLSA gene sequence and rpoB sequence were discordant (e. g., M. massiliense MLSA sequence and M. abscessus rpoB sequence), suggesting the intergroup lateral transfers of rpoB. in conclusion, our study strongly supports the recent proposal that M. abscessus, M. massiliense, and M. bolletii should constitute a single species. Our findings also indicate that there has been a horizontal transfer of rpoB sequences between these subgroups, precluding the use of rpoB sequencing alone for the accurate identification of the two proposed M. abscessus subspecies. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:06:06Z |
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2016-01-24T14:06:06Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 49, n. 2, p. 491-499, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33394 http://dx.doi.org/10.1128/JCM.01274-10 |
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0095-1137 |
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WOS000286977500003.pdf |
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10.1128/JCM.01274-10 |
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WOS:000286977500003 |
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Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 49, n. 2, p. 491-499, 2011. 0095-1137 WOS000286977500003.pdf 10.1128/JCM.01274-10 WOS:000286977500003 |
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http://repositorio.unifesp.br/handle/11600/33394 http://dx.doi.org/10.1128/JCM.01274-10 |
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eng |
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491-499 |
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Amer Soc Microbiology |
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Amer Soc Microbiology |
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