TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37087 http://dx.doi.org/10.1186/1479-5876-11-316 |
Resumo: | Background: Hypermethylation in the promoter regions is associated with the suppression of gene expression and has been considered a potential molecular marker for several tumor types, including head and neck squamous cell carcinomas (HNSCC).Methods: To evaluate the gene hypermethylation profile as a prognostic marker, this retrospective study used a QMSP approach to determine the methylation status of 19 genes in 70 HNSCC patients.Results: the methylation profile analysis of primary HNSCC revealed that genes CCNA1, DAPK, MGMT, TIMP3 and SFRP1 were frequently hypermethylated, with high specificity and sensitivity. TIMP3 and CCNA1 hypermethylation was significantly associated with lower rates of second primary tumor-free survival (p = 0.007 and p = 0.001; log-rank test, respectively).Conclusion: This study, for the first time, presents CCNA1 and TIMP3 hypermethylation as a helpful tool to identify HNSCC subjects at risk of developing second primary carcinomas. |
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Rettori, Marianna Marconato [UNIFESP]Carvalho, Ana Carolina de [UNIFESP]Bomfim Longo, Ana Luiza [UNIFESP]Oliveira, Cleyton Zanardo deKowalski, Luiz PauloCarvalho, Andre LopesVettore, Andre Luiz [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Barretos Canc HospAC Camargo HospDuke NUS Grad Med Sch2016-01-24T14:34:52Z2016-01-24T14:34:52Z2013-12-20Journal of Translational Medicine. London: Biomed Central Ltd, v. 11, 11 p., 2013.1479-5876http://repositorio.unifesp.br/handle/11600/37087http://dx.doi.org/10.1186/1479-5876-11-316WOS000329404100002.pdf10.1186/1479-5876-11-316WOS:000329404100002Background: Hypermethylation in the promoter regions is associated with the suppression of gene expression and has been considered a potential molecular marker for several tumor types, including head and neck squamous cell carcinomas (HNSCC).Methods: To evaluate the gene hypermethylation profile as a prognostic marker, this retrospective study used a QMSP approach to determine the methylation status of 19 genes in 70 HNSCC patients.Results: the methylation profile analysis of primary HNSCC revealed that genes CCNA1, DAPK, MGMT, TIMP3 and SFRP1 were frequently hypermethylated, with high specificity and sensitivity. TIMP3 and CCNA1 hypermethylation was significantly associated with lower rates of second primary tumor-free survival (p = 0.007 and p = 0.001; log-rank test, respectively).Conclusion: This study, for the first time, presents CCNA1 and TIMP3 hypermethylation as a helpful tool to identify HNSCC subjects at risk of developing second primary carcinomas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Biol Sci, Lab Mol Canc Biol, BR-04039020 São Paulo, BrazilBarretos Canc Hosp, Stat & Epidemiol Ctr, BR-14784400 Barretos, BrazilAC Camargo Hosp, Dept Head & Neck Surg, BR-01509010 São Paulo, BrazilBarretos Canc Hosp, Dept Head & Neck Surg, BR-14784400 Barretos, BrazilDuke NUS Grad Med Sch, Canc & Stem Cell Biol Program, Singapore 169857, SingaporeUniversidade Federal de São Paulo, Dept Biol Sci, Lab Mol Canc Biol, BR-04039020 São Paulo, BrazilWeb of Science11engBiomed Central LtdJournal of Translational MedicineHead and neck cancersHNSCC Prognostic MarkerDNA methylationepigeneticsTIMP3CCNA1TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000329404100002.pdfapplication/pdf413482${dspace.ui.url}/bitstream/11600/37087/1/WOS000329404100002.pdf726ab2bf04f49b47692080372cbe4cf7MD51open accessTEXTWOS000329404100002.pdf.txtWOS000329404100002.pdf.txtExtracted texttext/plain48432${dspace.ui.url}/bitstream/11600/37087/2/WOS000329404100002.pdf.txt94abb9c4da8cfd3486e34a8b70046918MD52open access11600/370872023-02-15 09:30:32.955open accessoai:repositorio.unifesp.br:11600/37087Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-02-15T12:30:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors |
title |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors |
spellingShingle |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors Rettori, Marianna Marconato [UNIFESP] Head and neck cancers HNSCC Prognostic Marker DNA methylation epigenetics TIMP3 CCNA1 |
title_short |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors |
title_full |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors |
title_fullStr |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors |
title_full_unstemmed |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors |
title_sort |
TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors |
author |
Rettori, Marianna Marconato [UNIFESP] |
author_facet |
Rettori, Marianna Marconato [UNIFESP] Carvalho, Ana Carolina de [UNIFESP] Bomfim Longo, Ana Luiza [UNIFESP] Oliveira, Cleyton Zanardo de Kowalski, Luiz Paulo Carvalho, Andre Lopes Vettore, Andre Luiz [UNIFESP] |
author_role |
author |
author2 |
Carvalho, Ana Carolina de [UNIFESP] Bomfim Longo, Ana Luiza [UNIFESP] Oliveira, Cleyton Zanardo de Kowalski, Luiz Paulo Carvalho, Andre Lopes Vettore, Andre Luiz [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Barretos Canc Hosp AC Camargo Hosp Duke NUS Grad Med Sch |
dc.contributor.author.fl_str_mv |
Rettori, Marianna Marconato [UNIFESP] Carvalho, Ana Carolina de [UNIFESP] Bomfim Longo, Ana Luiza [UNIFESP] Oliveira, Cleyton Zanardo de Kowalski, Luiz Paulo Carvalho, Andre Lopes Vettore, Andre Luiz [UNIFESP] |
dc.subject.eng.fl_str_mv |
Head and neck cancers HNSCC Prognostic Marker DNA methylation epigenetics TIMP3 CCNA1 |
topic |
Head and neck cancers HNSCC Prognostic Marker DNA methylation epigenetics TIMP3 CCNA1 |
description |
Background: Hypermethylation in the promoter regions is associated with the suppression of gene expression and has been considered a potential molecular marker for several tumor types, including head and neck squamous cell carcinomas (HNSCC).Methods: To evaluate the gene hypermethylation profile as a prognostic marker, this retrospective study used a QMSP approach to determine the methylation status of 19 genes in 70 HNSCC patients.Results: the methylation profile analysis of primary HNSCC revealed that genes CCNA1, DAPK, MGMT, TIMP3 and SFRP1 were frequently hypermethylated, with high specificity and sensitivity. TIMP3 and CCNA1 hypermethylation was significantly associated with lower rates of second primary tumor-free survival (p = 0.007 and p = 0.001; log-rank test, respectively).Conclusion: This study, for the first time, presents CCNA1 and TIMP3 hypermethylation as a helpful tool to identify HNSCC subjects at risk of developing second primary carcinomas. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-20 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:34:52Z |
dc.date.available.fl_str_mv |
2016-01-24T14:34:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Translational Medicine. London: Biomed Central Ltd, v. 11, 11 p., 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37087 http://dx.doi.org/10.1186/1479-5876-11-316 |
dc.identifier.issn.none.fl_str_mv |
1479-5876 |
dc.identifier.file.none.fl_str_mv |
WOS000329404100002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1479-5876-11-316 |
dc.identifier.wos.none.fl_str_mv |
WOS:000329404100002 |
identifier_str_mv |
Journal of Translational Medicine. London: Biomed Central Ltd, v. 11, 11 p., 2013. 1479-5876 WOS000329404100002.pdf 10.1186/1479-5876-11-316 WOS:000329404100002 |
url |
http://repositorio.unifesp.br/handle/11600/37087 http://dx.doi.org/10.1186/1479-5876-11-316 |
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eng |
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11 |
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Biomed Central Ltd |
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Biomed Central Ltd |
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