IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2

Detalhes bibliográficos
Autor(a) principal: Francescantonio, Paulo Luiz Carvalho
Data de Publicação: 2014
Outros Autores: Cruvinel, Wilson de Melo [UNIFESP], Dellavance, Alessandra [UNIFESP], Andrade, Luiz Eduardo Coelho [UNIFESP], Taliberti, Ben Hur, Von Mühlen, Carlos Alberto, Bichara, Carlos David Araújo, Bueno, Cleonice, Mangueira, Cristóvão Luis Pitangueiras, Carvalho, Darlene Gonçalves, Bonfá, Eloisa Silva Dutra de Oliveira, Brito, Fabiano de Almeida, Araújo, Flávia Ikeda e, Rego, Jozelia, Pereira, Kaline Medeiros Costa, Anjos, Lisiane Maria Enriconi dos, Bissoli, Maria de Fatima, Santiago, Mittermayer Barreto, Maluf, Natalya Zaidan, Alvarenga, Rossana Rassi, Neves, Suzane Pretti Figueiredo, Valim, Valeria, Santos, Wilton Silva dos [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000004rcc
Texto Completo: http://dx.doi.org/10.1016/j.rbre.2014.02.006
http://repositorio.unifesp.br/handle/11600/37118
Resumo: Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.
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spelling IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2IV Brazilian Guidelines for autoantibodies on HEp-2 cellsAutoantibodiesHEp-2 cellsAntinuclear antibodiesIndirect immunofluorescenceANA consensusAutoanticorposCélulas HEp-2Anticorpos antinúcleoImunofluorescência indiretaConsenso de FAN-HEp-2Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.Objetivo: O IV Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN) realizado em Vitória (ES), no dia 18 de setembro de 2012, objetivou discutir estratégias e recomendações relacionadas ao procedimento técnico, à padronização e à interpretação dos resultados da pesquisa de autoanticorpos em células HEp-2. Métodos: Participaram do evento 23 pesquisadores e especialistas de Universidades e laboratórios brasileiros. Foram abordados diferentes tópicos, discutidos amplamente a fim de se estabelecer recomendações específicas. Resultados e conclusão: O IV Consenso integrou à árvore de decisão o padrão citoplasmático em Anéis e Bastões, o padrão nuclear pontilhado Quasi-homogêneo (QH) e o padrão misto CENP-F. Discutiu-se ainda a necessidade de atenção para a classificação do padrão misto relacionado à presença de anticorpos anti-DNA topoisomerase I (Scl-70), compreendendo os componentes nuclear pontilhado fino, nucleolar homogêneo, NOR na placa metafásica e citoplasmático pontilhado fino. Foram sugeridas diretrizes para o controle de qualidade do teste, diluição de triagem e diluição de esgotamento, e foi emitido alerta quanto à necessidade de atenção em relação à heterogeneidade de substratos disponíveis no mercado e a utilização de metodologias automatizadas para detecção de autoanticorpos.PUC Goias, Goiania, Go, BrazilFleury Med & Saude, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, BrazilUniv Fed Uberlandia, Hosp Clin, Serv Rheumatol, BR-38400 Uberlandia, MG, BrazilClin Doencas Reumat Porto Alegre, Ctr Diagnost Med & Rheuma, Porto Alegre, RS, BrazilAmaral Costa Med Diagnost, Belem, PA, BrazilUniv São Paulo, Fac Med, Hosp Clin, Lab Invest Med, São Paulo, BrazilUniv São Paulo, Fac Med, Hosp Clin, Lab Cent, São Paulo, BrazilHosp Israelita Albert Einstein, Dept Clin Pathol, São Paulo, BrazilInst Hermes Pardini, Belo Horizonte, MG, BrazilUniv São Paulo, Fac Med, São Paulo, BrazilUniv Fed Minas Gerais, Fac Med, Belo Horizonte, MG, BrazilUniv Catolica Brasilia, Brasilia, DF, BrazilUniv Fed Goias, Fac Med, Goiania, Go, BrazilUniv Sul Santa Catarina UNISUL, Florianopolis, SC, BrazilUniv Vale do Itajai UNIVALE, Florianopolis, SC, BrazilUniv Fed Espirito Santo, Vitoria, ES, BrazilEBMSP, Salvador, BA, BrazilGrp DASA, São Paulo, BrazilEscola Super Ciencias Saude Dist Fed, Brasilia, DF, BrazilLab Sabin, Brasilia, DF, BrazilUniversidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, BrazilWeb of ScienceAlbert Einstein Medicina DiagnosticaAlka TecnologiaAmaral Costa LaboratorioConselho Federal de BiomedicinaDASAEuroimmun BrasilGrupo FleuryHemagenMedivaxOlimpusPadrao Laboratorio ClinicPontificia Universidade Catolica de Goias - PUC-GoiasSociedade Brasileira de Patologia Clinica e Medicina LaboratorialSociedade Brasileira de ReumatologiaThermo ScientificWama DiagnosticaWerfen Group - Werfen MedicalHermes PardiniConselho Regional de Biomedicina - 3a RegiaoElsevier B.V.PUC GoiasFleury Med & SaudeUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de Uberlândia (UFU)Clin Doencas Reumat Porto AlegreAmaral Costa Med DiagnostUniversidade de São Paulo (USP)Hosp Israelita Albert EinsteinInst Hermes PardiniUniversidade Federal de Minas Gerais (UFMG)Univ Catolica BrasiliaUniversidade Federal de Goiás (UFG)Univ Sul Santa Catarina UNISULUniv Vale do Itajai UNIVALEUniv Fed Espirito SantoEBMSPGrp DASAEscola Super Ciencias Saude Dist FedLab SabinFrancescantonio, Paulo Luiz CarvalhoCruvinel, Wilson de Melo [UNIFESP]Dellavance, Alessandra [UNIFESP]Andrade, Luiz Eduardo Coelho [UNIFESP]Taliberti, Ben HurVon Mühlen, Carlos AlbertoBichara, Carlos David AraújoBueno, CleoniceMangueira, Cristóvão Luis PitangueirasCarvalho, Darlene GonçalvesBonfá, Eloisa Silva Dutra de OliveiraBrito, Fabiano de AlmeidaAraújo, Flávia Ikeda eRego, JozeliaPereira, Kaline Medeiros CostaAnjos, Lisiane Maria Enriconi dosBissoli, Maria de FatimaSantiago, Mittermayer BarretoMaluf, Natalya ZaidanAlvarenga, Rossana RassiNeves, Suzane Pretti FigueiredoValim, ValeriaSantos, Wilton Silva dos [UNIFESP]2016-01-24T14:34:55Z2016-01-24T14:34:55Z2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion44-50application/pdfapplication/pdfhttp://dx.doi.org/10.1016/j.rbre.2014.02.006Revista Brasileira de Reumatologia. New York: Elsevier B.V., v. 54, n. 1, p. 44-50, 2014.10.1016/j.rbre.2014.02.006S0482-50042014000100044-en.pdf|S0482-50042014000100044-pt.pdf0482-5004S0482-50042014000100044http://repositorio.unifesp.br/handle/11600/37118WOS:000332910600009ark:/48912/0013000004rccengporRevista Brasileira de Reumatologiainfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T03:24:38Zoai:repositorio.unifesp.br/:11600/37118Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T19:57:32.881941Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
IV Brazilian Guidelines for autoantibodies on HEp-2 cells
title IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
spellingShingle IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
Francescantonio, Paulo Luiz Carvalho
Autoantibodies
HEp-2 cells
Antinuclear antibodies
Indirect immunofluorescence
ANA consensus
Autoanticorpos
Células HEp-2
Anticorpos antinúcleo
Imunofluorescência indireta
Consenso de FAN-HEp-2
title_short IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
title_full IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
title_fullStr IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
title_full_unstemmed IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
title_sort IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
author Francescantonio, Paulo Luiz Carvalho
author_facet Francescantonio, Paulo Luiz Carvalho
Cruvinel, Wilson de Melo [UNIFESP]
Dellavance, Alessandra [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
Taliberti, Ben Hur
Von Mühlen, Carlos Alberto
Bichara, Carlos David Araújo
Bueno, Cleonice
Mangueira, Cristóvão Luis Pitangueiras
Carvalho, Darlene Gonçalves
Bonfá, Eloisa Silva Dutra de Oliveira
Brito, Fabiano de Almeida
Araújo, Flávia Ikeda e
Rego, Jozelia
Pereira, Kaline Medeiros Costa
Anjos, Lisiane Maria Enriconi dos
Bissoli, Maria de Fatima
Santiago, Mittermayer Barreto
Maluf, Natalya Zaidan
Alvarenga, Rossana Rassi
Neves, Suzane Pretti Figueiredo
Valim, Valeria
Santos, Wilton Silva dos [UNIFESP]
author_role author
author2 Cruvinel, Wilson de Melo [UNIFESP]
Dellavance, Alessandra [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
Taliberti, Ben Hur
Von Mühlen, Carlos Alberto
Bichara, Carlos David Araújo
Bueno, Cleonice
Mangueira, Cristóvão Luis Pitangueiras
Carvalho, Darlene Gonçalves
Bonfá, Eloisa Silva Dutra de Oliveira
Brito, Fabiano de Almeida
Araújo, Flávia Ikeda e
Rego, Jozelia
Pereira, Kaline Medeiros Costa
Anjos, Lisiane Maria Enriconi dos
Bissoli, Maria de Fatima
Santiago, Mittermayer Barreto
Maluf, Natalya Zaidan
Alvarenga, Rossana Rassi
Neves, Suzane Pretti Figueiredo
Valim, Valeria
Santos, Wilton Silva dos [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv PUC Goias
Fleury Med & Saude
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Uberlândia (UFU)
Clin Doencas Reumat Porto Alegre
Amaral Costa Med Diagnost
Universidade de São Paulo (USP)
Hosp Israelita Albert Einstein
Inst Hermes Pardini
Universidade Federal de Minas Gerais (UFMG)
Univ Catolica Brasilia
Universidade Federal de Goiás (UFG)
Univ Sul Santa Catarina UNISUL
Univ Vale do Itajai UNIVALE
Univ Fed Espirito Santo
EBMSP
Grp DASA
Escola Super Ciencias Saude Dist Fed
Lab Sabin
dc.contributor.author.fl_str_mv Francescantonio, Paulo Luiz Carvalho
Cruvinel, Wilson de Melo [UNIFESP]
Dellavance, Alessandra [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
Taliberti, Ben Hur
Von Mühlen, Carlos Alberto
Bichara, Carlos David Araújo
Bueno, Cleonice
Mangueira, Cristóvão Luis Pitangueiras
Carvalho, Darlene Gonçalves
Bonfá, Eloisa Silva Dutra de Oliveira
Brito, Fabiano de Almeida
Araújo, Flávia Ikeda e
Rego, Jozelia
Pereira, Kaline Medeiros Costa
Anjos, Lisiane Maria Enriconi dos
Bissoli, Maria de Fatima
Santiago, Mittermayer Barreto
Maluf, Natalya Zaidan
Alvarenga, Rossana Rassi
Neves, Suzane Pretti Figueiredo
Valim, Valeria
Santos, Wilton Silva dos [UNIFESP]
dc.subject.por.fl_str_mv Autoantibodies
HEp-2 cells
Antinuclear antibodies
Indirect immunofluorescence
ANA consensus
Autoanticorpos
Células HEp-2
Anticorpos antinúcleo
Imunofluorescência indireta
Consenso de FAN-HEp-2
topic Autoantibodies
HEp-2 cells
Antinuclear antibodies
Indirect immunofluorescence
ANA consensus
Autoanticorpos
Células HEp-2
Anticorpos antinúcleo
Imunofluorescência indireta
Consenso de FAN-HEp-2
description Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2016-01-24T14:34:55Z
2016-01-24T14:34:55Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.rbre.2014.02.006
Revista Brasileira de Reumatologia. New York: Elsevier B.V., v. 54, n. 1, p. 44-50, 2014.
10.1016/j.rbre.2014.02.006
S0482-50042014000100044-en.pdf|S0482-50042014000100044-pt.pdf
0482-5004
S0482-50042014000100044
http://repositorio.unifesp.br/handle/11600/37118
WOS:000332910600009
dc.identifier.dark.fl_str_mv ark:/48912/0013000004rcc
url http://dx.doi.org/10.1016/j.rbre.2014.02.006
http://repositorio.unifesp.br/handle/11600/37118
identifier_str_mv Revista Brasileira de Reumatologia. New York: Elsevier B.V., v. 54, n. 1, p. 44-50, 2014.
10.1016/j.rbre.2014.02.006
S0482-50042014000100044-en.pdf|S0482-50042014000100044-pt.pdf
0482-5004
S0482-50042014000100044
WOS:000332910600009
ark:/48912/0013000004rcc
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista Brasileira de Reumatologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 44-50
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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