IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/0013000004rcc |
Texto Completo: | http://dx.doi.org/10.1016/j.rbre.2014.02.006 http://repositorio.unifesp.br/handle/11600/37118 |
Resumo: | Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved. |
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IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2IV Brazilian Guidelines for autoantibodies on HEp-2 cellsAutoantibodiesHEp-2 cellsAntinuclear antibodiesIndirect immunofluorescenceANA consensusAutoanticorposCélulas HEp-2Anticorpos antinúcleoImunofluorescência indiretaConsenso de FAN-HEp-2Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.Objetivo: O IV Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN) realizado em Vitória (ES), no dia 18 de setembro de 2012, objetivou discutir estratégias e recomendações relacionadas ao procedimento técnico, à padronização e à interpretação dos resultados da pesquisa de autoanticorpos em células HEp-2. Métodos: Participaram do evento 23 pesquisadores e especialistas de Universidades e laboratórios brasileiros. Foram abordados diferentes tópicos, discutidos amplamente a fim de se estabelecer recomendações específicas. Resultados e conclusão: O IV Consenso integrou à árvore de decisão o padrão citoplasmático em Anéis e Bastões, o padrão nuclear pontilhado Quasi-homogêneo (QH) e o padrão misto CENP-F. Discutiu-se ainda a necessidade de atenção para a classificação do padrão misto relacionado à presença de anticorpos anti-DNA topoisomerase I (Scl-70), compreendendo os componentes nuclear pontilhado fino, nucleolar homogêneo, NOR na placa metafásica e citoplasmático pontilhado fino. Foram sugeridas diretrizes para o controle de qualidade do teste, diluição de triagem e diluição de esgotamento, e foi emitido alerta quanto à necessidade de atenção em relação à heterogeneidade de substratos disponíveis no mercado e a utilização de metodologias automatizadas para detecção de autoanticorpos.PUC Goias, Goiania, Go, BrazilFleury Med & Saude, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, BrazilUniv Fed Uberlandia, Hosp Clin, Serv Rheumatol, BR-38400 Uberlandia, MG, BrazilClin Doencas Reumat Porto Alegre, Ctr Diagnost Med & Rheuma, Porto Alegre, RS, BrazilAmaral Costa Med Diagnost, Belem, PA, BrazilUniv São Paulo, Fac Med, Hosp Clin, Lab Invest Med, São Paulo, BrazilUniv São Paulo, Fac Med, Hosp Clin, Lab Cent, São Paulo, BrazilHosp Israelita Albert Einstein, Dept Clin Pathol, São Paulo, BrazilInst Hermes Pardini, Belo Horizonte, MG, BrazilUniv São Paulo, Fac Med, São Paulo, BrazilUniv Fed Minas Gerais, Fac Med, Belo Horizonte, MG, BrazilUniv Catolica Brasilia, Brasilia, DF, BrazilUniv Fed Goias, Fac Med, Goiania, Go, BrazilUniv Sul Santa Catarina UNISUL, Florianopolis, SC, BrazilUniv Vale do Itajai UNIVALE, Florianopolis, SC, BrazilUniv Fed Espirito Santo, Vitoria, ES, BrazilEBMSP, Salvador, BA, BrazilGrp DASA, São Paulo, BrazilEscola Super Ciencias Saude Dist Fed, Brasilia, DF, BrazilLab Sabin, Brasilia, DF, BrazilUniversidade Federal de São Paulo UNIFESP, Discipline Rheumatol, São Paulo, BrazilWeb of ScienceAlbert Einstein Medicina DiagnosticaAlka TecnologiaAmaral Costa LaboratorioConselho Federal de BiomedicinaDASAEuroimmun BrasilGrupo FleuryHemagenMedivaxOlimpusPadrao Laboratorio ClinicPontificia Universidade Catolica de Goias - PUC-GoiasSociedade Brasileira de Patologia Clinica e Medicina LaboratorialSociedade Brasileira de ReumatologiaThermo ScientificWama DiagnosticaWerfen Group - Werfen MedicalHermes PardiniConselho Regional de Biomedicina - 3a RegiaoElsevier B.V.PUC GoiasFleury Med & SaudeUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de Uberlândia (UFU)Clin Doencas Reumat Porto AlegreAmaral Costa Med DiagnostUniversidade de São Paulo (USP)Hosp Israelita Albert EinsteinInst Hermes PardiniUniversidade Federal de Minas Gerais (UFMG)Univ Catolica BrasiliaUniversidade Federal de Goiás (UFG)Univ Sul Santa Catarina UNISULUniv Vale do Itajai UNIVALEUniv Fed Espirito SantoEBMSPGrp DASAEscola Super Ciencias Saude Dist FedLab SabinFrancescantonio, Paulo Luiz CarvalhoCruvinel, Wilson de Melo [UNIFESP]Dellavance, Alessandra [UNIFESP]Andrade, Luiz Eduardo Coelho [UNIFESP]Taliberti, Ben HurVon Mühlen, Carlos AlbertoBichara, Carlos David AraújoBueno, CleoniceMangueira, Cristóvão Luis PitangueirasCarvalho, Darlene GonçalvesBonfá, Eloisa Silva Dutra de OliveiraBrito, Fabiano de AlmeidaAraújo, Flávia Ikeda eRego, JozeliaPereira, Kaline Medeiros CostaAnjos, Lisiane Maria Enriconi dosBissoli, Maria de FatimaSantiago, Mittermayer BarretoMaluf, Natalya ZaidanAlvarenga, Rossana RassiNeves, Suzane Pretti FigueiredoValim, ValeriaSantos, Wilton Silva dos [UNIFESP]2016-01-24T14:34:55Z2016-01-24T14:34:55Z2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion44-50application/pdfapplication/pdfhttp://dx.doi.org/10.1016/j.rbre.2014.02.006Revista Brasileira de Reumatologia. New York: Elsevier B.V., v. 54, n. 1, p. 44-50, 2014.10.1016/j.rbre.2014.02.006S0482-50042014000100044-en.pdf|S0482-50042014000100044-pt.pdf0482-5004S0482-50042014000100044http://repositorio.unifesp.br/handle/11600/37118WOS:000332910600009ark:/48912/0013000004rccengporRevista Brasileira de Reumatologiainfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T03:24:38Zoai:repositorio.unifesp.br/:11600/37118Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T19:57:32.881941Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 IV Brazilian Guidelines for autoantibodies on HEp-2 cells |
title |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 |
spellingShingle |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 Francescantonio, Paulo Luiz Carvalho Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence ANA consensus Autoanticorpos Células HEp-2 Anticorpos antinúcleo Imunofluorescência indireta Consenso de FAN-HEp-2 |
title_short |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 |
title_full |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 |
title_fullStr |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 |
title_full_unstemmed |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 |
title_sort |
IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2 |
author |
Francescantonio, Paulo Luiz Carvalho |
author_facet |
Francescantonio, Paulo Luiz Carvalho Cruvinel, Wilson de Melo [UNIFESP] Dellavance, Alessandra [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] Taliberti, Ben Hur Von Mühlen, Carlos Alberto Bichara, Carlos David Araújo Bueno, Cleonice Mangueira, Cristóvão Luis Pitangueiras Carvalho, Darlene Gonçalves Bonfá, Eloisa Silva Dutra de Oliveira Brito, Fabiano de Almeida Araújo, Flávia Ikeda e Rego, Jozelia Pereira, Kaline Medeiros Costa Anjos, Lisiane Maria Enriconi dos Bissoli, Maria de Fatima Santiago, Mittermayer Barreto Maluf, Natalya Zaidan Alvarenga, Rossana Rassi Neves, Suzane Pretti Figueiredo Valim, Valeria Santos, Wilton Silva dos [UNIFESP] |
author_role |
author |
author2 |
Cruvinel, Wilson de Melo [UNIFESP] Dellavance, Alessandra [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] Taliberti, Ben Hur Von Mühlen, Carlos Alberto Bichara, Carlos David Araújo Bueno, Cleonice Mangueira, Cristóvão Luis Pitangueiras Carvalho, Darlene Gonçalves Bonfá, Eloisa Silva Dutra de Oliveira Brito, Fabiano de Almeida Araújo, Flávia Ikeda e Rego, Jozelia Pereira, Kaline Medeiros Costa Anjos, Lisiane Maria Enriconi dos Bissoli, Maria de Fatima Santiago, Mittermayer Barreto Maluf, Natalya Zaidan Alvarenga, Rossana Rassi Neves, Suzane Pretti Figueiredo Valim, Valeria Santos, Wilton Silva dos [UNIFESP] |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
PUC Goias Fleury Med & Saude Universidade Federal de São Paulo (UNIFESP) Universidade Federal de Uberlândia (UFU) Clin Doencas Reumat Porto Alegre Amaral Costa Med Diagnost Universidade de São Paulo (USP) Hosp Israelita Albert Einstein Inst Hermes Pardini Universidade Federal de Minas Gerais (UFMG) Univ Catolica Brasilia Universidade Federal de Goiás (UFG) Univ Sul Santa Catarina UNISUL Univ Vale do Itajai UNIVALE Univ Fed Espirito Santo EBMSP Grp DASA Escola Super Ciencias Saude Dist Fed Lab Sabin |
dc.contributor.author.fl_str_mv |
Francescantonio, Paulo Luiz Carvalho Cruvinel, Wilson de Melo [UNIFESP] Dellavance, Alessandra [UNIFESP] Andrade, Luiz Eduardo Coelho [UNIFESP] Taliberti, Ben Hur Von Mühlen, Carlos Alberto Bichara, Carlos David Araújo Bueno, Cleonice Mangueira, Cristóvão Luis Pitangueiras Carvalho, Darlene Gonçalves Bonfá, Eloisa Silva Dutra de Oliveira Brito, Fabiano de Almeida Araújo, Flávia Ikeda e Rego, Jozelia Pereira, Kaline Medeiros Costa Anjos, Lisiane Maria Enriconi dos Bissoli, Maria de Fatima Santiago, Mittermayer Barreto Maluf, Natalya Zaidan Alvarenga, Rossana Rassi Neves, Suzane Pretti Figueiredo Valim, Valeria Santos, Wilton Silva dos [UNIFESP] |
dc.subject.por.fl_str_mv |
Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence ANA consensus Autoanticorpos Células HEp-2 Anticorpos antinúcleo Imunofluorescência indireta Consenso de FAN-HEp-2 |
topic |
Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence ANA consensus Autoanticorpos Células HEp-2 Anticorpos antinúcleo Imunofluorescência indireta Consenso de FAN-HEp-2 |
description |
Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2016-01-24T14:34:55Z 2016-01-24T14:34:55Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rbre.2014.02.006 Revista Brasileira de Reumatologia. New York: Elsevier B.V., v. 54, n. 1, p. 44-50, 2014. 10.1016/j.rbre.2014.02.006 S0482-50042014000100044-en.pdf|S0482-50042014000100044-pt.pdf 0482-5004 S0482-50042014000100044 http://repositorio.unifesp.br/handle/11600/37118 WOS:000332910600009 |
dc.identifier.dark.fl_str_mv |
ark:/48912/0013000004rcc |
url |
http://dx.doi.org/10.1016/j.rbre.2014.02.006 http://repositorio.unifesp.br/handle/11600/37118 |
identifier_str_mv |
Revista Brasileira de Reumatologia. New York: Elsevier B.V., v. 54, n. 1, p. 44-50, 2014. 10.1016/j.rbre.2014.02.006 S0482-50042014000100044-en.pdf|S0482-50042014000100044-pt.pdf 0482-5004 S0482-50042014000100044 WOS:000332910600009 ark:/48912/0013000004rcc |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Reumatologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
44-50 application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1818602403113467904 |