Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/11600/42476 http://www.ajtmh.org/content/65/3/237.abstract |
Resumo: | Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved. |
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Repositório Institucional da UNIFESP |
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Mamoni, Ronei L.Rossi, C. L.Camargo, Zoilo Pires de [UNIFESP]Blotta, Maria Heloisa de Souza Lima [UNIFESP]Universidade Estadual de Campinas (UNICAMP)Universidade Federal de São Paulo (UNIFESP)2018-06-15T13:46:00Z2018-06-15T13:46:00Z2001-09-01American Journal Of Tropical Medicine And Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 65, n. 3, p. 237-241, 2001.0002-9637http://repositorio.unifesp.br/11600/42476http://www.ajtmh.org/content/65/3/237.abstractWOS:000170933400015Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved.State Univ Campinas, Fac Med Sci, Dept Clin Pathol, BR-13083970 Campinas, SP, BrazilUniv Fed Sao Paulo, Discipline Cellular Biol, Sao Paulo, BrazilUniv Fed Sao Paulo, Discipline Cellular Biol, Sao Paulo, BrazilWeb of Science237-241engAmer Soc Trop Med & HygieneAmerican Journal Of Tropical Medicine And HygieneCapture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/424762021-10-05 22:00:45.27metadata only accessoai:repositorio.unifesp.br:11600/42476Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-10-06T01:00:45Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis |
title |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis |
spellingShingle |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis Mamoni, Ronei L. |
title_short |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis |
title_full |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis |
title_fullStr |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis |
title_full_unstemmed |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis |
title_sort |
Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis |
author |
Mamoni, Ronei L. |
author_facet |
Mamoni, Ronei L. Rossi, C. L. Camargo, Zoilo Pires de [UNIFESP] Blotta, Maria Heloisa de Souza Lima [UNIFESP] |
author_role |
author |
author2 |
Rossi, C. L. Camargo, Zoilo Pires de [UNIFESP] Blotta, Maria Heloisa de Souza Lima [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Mamoni, Ronei L. Rossi, C. L. Camargo, Zoilo Pires de [UNIFESP] Blotta, Maria Heloisa de Souza Lima [UNIFESP] |
description |
Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-09-01 |
dc.date.accessioned.fl_str_mv |
2018-06-15T13:46:00Z |
dc.date.available.fl_str_mv |
2018-06-15T13:46:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
American Journal Of Tropical Medicine And Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 65, n. 3, p. 237-241, 2001. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/11600/42476 http://www.ajtmh.org/content/65/3/237.abstract |
dc.identifier.issn.none.fl_str_mv |
0002-9637 |
dc.identifier.wos.none.fl_str_mv |
WOS:000170933400015 |
identifier_str_mv |
American Journal Of Tropical Medicine And Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 65, n. 3, p. 237-241, 2001. 0002-9637 WOS:000170933400015 |
url |
http://repositorio.unifesp.br/11600/42476 http://www.ajtmh.org/content/65/3/237.abstract |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
American Journal Of Tropical Medicine And Hygiene |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
237-241 |
dc.publisher.none.fl_str_mv |
Amer Soc Trop Med & Hygiene |
publisher.none.fl_str_mv |
Amer Soc Trop Med & Hygiene |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764117378859008 |