Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

• Autor(a) principal: Calcagno, Danielle Queiroz [UNIFESP]
• Data de Publicação: 2006
• Outros Autores: Leal, Mariana Ferreira [UNIFESP], Seabra, Aline Damaceno, Khayat, Andre Salim, Chen, Elizabeth Suchi [UNIFESP], Demachki, Samia, Assumpção, Paulo Pimentel [UNIFESP], Girao Faria, Mario Henrique, Rabenhorst, Silvia Helena Barem, Ferreira, Márcia Valéria Pitombeira, Cardoso Smith, Marilia de Arruda [UNIFESP], Burbano, Rommel Rodriguez [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.3748/WJG.v12.i38.6207; http://repositorio.unifesp.br/handle/11600/45742
• topic: chromosome 8 aneuploidy; C-MYC amplification; immunostaining; gastric adenocarcinoma

AIM: To investigate chromosome 8 numerical aberrations, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histopathological characteristics of gastric tumors.METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed.RESULTS: All the cases showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level of chromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal-type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm.CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic pathways. (C) 2006 The WJG Press. All rights reserved.