Epitope Sequences in Dengue Virus NS1 Protein Identified by Monoclonal Antibodies

• Autor(a) principal: Rocha, Leticia Barboza
• Data de Publicação: 2017
• Outros Autores: dos Santos Alves, Rubens Prince, Caetano, Bruna Alves, Pereira, Lennon Ramos, Mitsunari, Thais, Amorim, Jaime Henrique, Polatto, Juliana Moutinho, Botosso, Viviane Fongaro, Frazatti Gallina, Neuza Maria, Palacios, Ricardo, Precioso, Alexander Roberto, Hernandes Granato, Celso Francisco [UNIFESP], Leal Oliveira, Danielle Bruna, da Silveira, Vanessa Barbosa, Luz, Daniela, de Souza Ferreira, Luis Carlos, Fontes Piazza, Roxane Maria
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.3390/antib6040014; https://repositorio.unifesp.br/handle/11600/58058
• topic: dengue virus; NS1; Zika virus; mAbs; antibody recognition; amino acid sequences

Dengue nonstructural protein 1 (NS1) is a multi-functional glycoprotein with essential functions both in viral replication and modulation of host innate immune responses. NS1 has been established as a good surrogate marker for infection. In the present study, we generated four anti-NS1 monoclonal antibodies against recombinant NS1 protein from dengue virus serotype 2 (DENV2), which were used to map three NS1 epitopes. The sequence (193)AVHADMGYWIESALNDT(209) was recognized by monoclonal antibodies 2H5 and 4H1BC, which also cross-reacted with Zika virus (ZIKV) protein. On the other hand, the sequence (25)VHTWTEQYKFQPES(38) was recognized by mAb 4F6 that did not cross react with ZIKV. Lastly, a previously unidentified DENV2 NS1-specific epitope, represented by the sequence (127)ELHNQTFLIDGPETAEC(143), is described in the present study after reaction with mAb 4H2, which also did not cross react with ZIKV. The selection and characterization of the epitope, specificity of anti-NS1 mAbs, may contribute to the development of diagnostic tools able to differentiate DENV and ZIKV infections.