The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/33018 http://dx.doi.org/10.1590/S0102-86502010000600008 |
Resumo: | Purpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand. |
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Bussuan, Luiz Antonio Maksoud [UNIFESP]Fagundes, Djalma Jose [UNIFESP]Marks, GuidoBussuan, Priscila MaksoudTeruya, RobertoUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de Mato Grosso do Sul (UFMS)Moreira Salles Inst IMS2016-01-24T14:05:37Z2016-01-24T14:05:37Z2010-11-01Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010.0102-8650http://repositorio.unifesp.br/handle/11600/33018http://dx.doi.org/10.1590/S0102-86502010000600008S0102-8650201000060000810.1590/S0102-86502010000600008WOS:000284779000008Purpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.UNIFESP SP, Dept Surg, Postgrad Program Surg & Experimentat, Div Operat Tech & Expt Surg, São Paulo, BrazilUFMS, Dept Surg, Campo Grande, MS USAMoreira Salles Inst IMS, Rio de Janeiro, BrazilFed Univ Mato Grosso Sul UFMS, Expt Carcinogenesis Lab, Cent Anim Facil, Campo Grande, MS USAFed Univ São Paulo UNIFESP, Surg & Experimentat Postgrad Program, São Paulo, BrazilUNIFESP SP, Dept Surg, Postgrad Program Surg & Experimentat, Div Operat Tech & Expt Surg, São Paulo, BrazilFed Univ São Paulo UNIFESP, Surg & Experimentat Postgrad Program, São Paulo, BrazilWeb of Science501-506engActa Cirurgica BrasileiraActa Cirurgica BrasileiraFas Ligand ProteinAzoxymethaneOxidative StressApoptosisRatsThe role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of ratsO papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0102-86502010000600008.pdfS0102-86502010000600008.pdfapplication/pdf302283${dspace.ui.url}/bitstream/11600/33018/1/S0102-86502010000600008.pdf6576bc4d4ba5f907c642428dcfe9ca47MD51open access11600/330182021-09-30 11:03:31.245open accessoai:repositorio.unifesp.br:11600/33018Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:18:30.215356Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats |
dc.title.alternative.pt.fl_str_mv |
O papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratos |
title |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats |
spellingShingle |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats Bussuan, Luiz Antonio Maksoud [UNIFESP] Fas Ligand Protein Azoxymethane Oxidative Stress Apoptosis Rats |
title_short |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats |
title_full |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats |
title_fullStr |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats |
title_full_unstemmed |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats |
title_sort |
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats |
author |
Bussuan, Luiz Antonio Maksoud [UNIFESP] |
author_facet |
Bussuan, Luiz Antonio Maksoud [UNIFESP] Fagundes, Djalma Jose [UNIFESP] Marks, Guido Bussuan, Priscila Maksoud Teruya, Roberto |
author_role |
author |
author2 |
Fagundes, Djalma Jose [UNIFESP] Marks, Guido Bussuan, Priscila Maksoud Teruya, Roberto |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Federal de Mato Grosso do Sul (UFMS) Moreira Salles Inst IMS |
dc.contributor.author.fl_str_mv |
Bussuan, Luiz Antonio Maksoud [UNIFESP] Fagundes, Djalma Jose [UNIFESP] Marks, Guido Bussuan, Priscila Maksoud Teruya, Roberto |
dc.subject.eng.fl_str_mv |
Fas Ligand Protein Azoxymethane Oxidative Stress Apoptosis Rats |
topic |
Fas Ligand Protein Azoxymethane Oxidative Stress Apoptosis Rats |
description |
Purpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-11-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:05:37Z |
dc.date.available.fl_str_mv |
2016-01-24T14:05:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/33018 http://dx.doi.org/10.1590/S0102-86502010000600008 |
dc.identifier.issn.none.fl_str_mv |
0102-8650 |
dc.identifier.scielo.none.fl_str_mv |
S0102-86502010000600008 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0102-86502010000600008 |
dc.identifier.wos.none.fl_str_mv |
WOS:000284779000008 |
identifier_str_mv |
Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010. 0102-8650 S0102-86502010000600008 10.1590/S0102-86502010000600008 WOS:000284779000008 |
url |
http://repositorio.unifesp.br/handle/11600/33018 http://dx.doi.org/10.1590/S0102-86502010000600008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
501-506 |
dc.publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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