Fractional CO2 Laser-Assisted Drug Delivery
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/32183 http://dx.doi.org/10.1002/lsm.20860 |
Resumo: | Background and Objectives: Ablative fractional resurfacing (AFR) creates vertical channels that might assist the delivery of topically applied drugs into skin. the purpose of this study was to evaluate drug delivery by CO2 laser AFR using methyl 5-aminolevulinate (MAL), a porphyrin precursor, as a test drug.Materials and Methods: Two Yorkshire swine were treated with single-hole CO2 laser AFR and subsequent topical application of MAL (Metvix(R), Photocure ASA, Oslo, Not-way), placebo cream and no drug. MAL-induced porphyrin fluorescence was measured by fluorescence microscopy at skin depths down to 1,800 mu m. AFR was performed with a 10.6 mu m wavelength Prototype CO2 laser, using stacked single pulses of 3 millisecond and 91.6 mJ pet, pulse.Results: AFR created cone-shaped channels of approximately 300 mu m diameter and 1,850 mu m depth that were Surrounded by a 70 mu m thin layer of thermally coagulated dermis. There was no porphyrin fluorescence in placebo cream or untreated skin sites. AFR followed by MAL application enhanced drug delivery with significantly higher porphyrin fluorescence of hair follicles (P < 0.0011) and dermis (P < 0.0433) versus MAL alone at skin depths of 120, 500, 1,000, 1,500, and 1,800 pro. AFR before MAL application also enhanced skin surface (epidermal) porphyrin fluorescence. Radial diffusion of MAL from the laser-created channels into surrounding dermis was evidenced by uniform porphyrin fluorescence up to 1,500 mu m from the holes (1,000, 1,800 mu m depths). Skin massage after MAL application did not affect MAL-induced porphyrin fluorescence after AFR.Conclusions: Ablative fractional laser treatment facilitates delivery of topical MAL deeply into the skin. for the conditions of this study, laser channels approximately 3 mm apart followed by MAL application could produce porphyrins throughout essentially the entire skin. AFR appears to be a clinically practical means for enhancing uptake of MAL, a photodynamic therapy drug, and presumably many other topical skin medications. Lasers Surg. Med. 42:113-122, 2010. (C) 2009Wiley-Liss, Inc. |
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Haedersdal, MereteSakamoto, Fernanda Hidemi [UNIFESP]Farinelli, William A.Doukas, Apostolos G.Tam, JoshAnderson, R. RoxHarvard UnivUniv CopenhagenUniversidade Federal de São Paulo (UNIFESP)2016-01-24T13:59:11Z2016-01-24T13:59:11Z2010-02-01Lasers in Surgery and Medicine. Malden: Wiley-Blackwell, v. 42, n. 2, p. 113-122, 2010.0196-8092http://repositorio.unifesp.br/handle/11600/32183http://dx.doi.org/10.1002/lsm.2086010.1002/lsm.20860WOS:000275449600004Background and Objectives: Ablative fractional resurfacing (AFR) creates vertical channels that might assist the delivery of topically applied drugs into skin. the purpose of this study was to evaluate drug delivery by CO2 laser AFR using methyl 5-aminolevulinate (MAL), a porphyrin precursor, as a test drug.Materials and Methods: Two Yorkshire swine were treated with single-hole CO2 laser AFR and subsequent topical application of MAL (Metvix(R), Photocure ASA, Oslo, Not-way), placebo cream and no drug. MAL-induced porphyrin fluorescence was measured by fluorescence microscopy at skin depths down to 1,800 mu m. AFR was performed with a 10.6 mu m wavelength Prototype CO2 laser, using stacked single pulses of 3 millisecond and 91.6 mJ pet, pulse.Results: AFR created cone-shaped channels of approximately 300 mu m diameter and 1,850 mu m depth that were Surrounded by a 70 mu m thin layer of thermally coagulated dermis. There was no porphyrin fluorescence in placebo cream or untreated skin sites. AFR followed by MAL application enhanced drug delivery with significantly higher porphyrin fluorescence of hair follicles (P < 0.0011) and dermis (P < 0.0433) versus MAL alone at skin depths of 120, 500, 1,000, 1,500, and 1,800 pro. AFR before MAL application also enhanced skin surface (epidermal) porphyrin fluorescence. Radial diffusion of MAL from the laser-created channels into surrounding dermis was evidenced by uniform porphyrin fluorescence up to 1,500 mu m from the holes (1,000, 1,800 mu m depths). Skin massage after MAL application did not affect MAL-induced porphyrin fluorescence after AFR.Conclusions: Ablative fractional laser treatment facilitates delivery of topical MAL deeply into the skin. for the conditions of this study, laser channels approximately 3 mm apart followed by MAL application could produce porphyrins throughout essentially the entire skin. AFR appears to be a clinically practical means for enhancing uptake of MAL, a photodynamic therapy drug, and presumably many other topical skin medications. Lasers Surg. Med. 42:113-122, 2010. (C) 2009Wiley-Liss, Inc.Harvard Univ, Sch Med, Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USAUniv Copenhagen, Dept Dermatol, Bispebjerg Hosp, DK-2400 Copenhagen, DenmarkUniversidade Federal de São Paulo, Dept Dermatol, Escola Paulista Med, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Dermatol, Escola Paulista Med, BR-04023900 São Paulo, BrazilWeb of Science113-122engWiley-BlackwellLasers in Surgery and Medicinehttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessfractional ablative resurfacingphotodynamic therapyphotosensitizertopical drugsFractional CO2 Laser-Assisted Drug Deliveryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/321832023-01-30 22:17:47.073metadata only accessoai:repositorio.unifesp.br:11600/32183Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-31T01:17:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Fractional CO2 Laser-Assisted Drug Delivery |
title |
Fractional CO2 Laser-Assisted Drug Delivery |
spellingShingle |
Fractional CO2 Laser-Assisted Drug Delivery Haedersdal, Merete fractional ablative resurfacing photodynamic therapy photosensitizer topical drugs |
title_short |
Fractional CO2 Laser-Assisted Drug Delivery |
title_full |
Fractional CO2 Laser-Assisted Drug Delivery |
title_fullStr |
Fractional CO2 Laser-Assisted Drug Delivery |
title_full_unstemmed |
Fractional CO2 Laser-Assisted Drug Delivery |
title_sort |
Fractional CO2 Laser-Assisted Drug Delivery |
author |
Haedersdal, Merete |
author_facet |
Haedersdal, Merete Sakamoto, Fernanda Hidemi [UNIFESP] Farinelli, William A. Doukas, Apostolos G. Tam, Josh Anderson, R. Rox |
author_role |
author |
author2 |
Sakamoto, Fernanda Hidemi [UNIFESP] Farinelli, William A. Doukas, Apostolos G. Tam, Josh Anderson, R. Rox |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Harvard Univ Univ Copenhagen Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Haedersdal, Merete Sakamoto, Fernanda Hidemi [UNIFESP] Farinelli, William A. Doukas, Apostolos G. Tam, Josh Anderson, R. Rox |
dc.subject.eng.fl_str_mv |
fractional ablative resurfacing photodynamic therapy photosensitizer topical drugs |
topic |
fractional ablative resurfacing photodynamic therapy photosensitizer topical drugs |
description |
Background and Objectives: Ablative fractional resurfacing (AFR) creates vertical channels that might assist the delivery of topically applied drugs into skin. the purpose of this study was to evaluate drug delivery by CO2 laser AFR using methyl 5-aminolevulinate (MAL), a porphyrin precursor, as a test drug.Materials and Methods: Two Yorkshire swine were treated with single-hole CO2 laser AFR and subsequent topical application of MAL (Metvix(R), Photocure ASA, Oslo, Not-way), placebo cream and no drug. MAL-induced porphyrin fluorescence was measured by fluorescence microscopy at skin depths down to 1,800 mu m. AFR was performed with a 10.6 mu m wavelength Prototype CO2 laser, using stacked single pulses of 3 millisecond and 91.6 mJ pet, pulse.Results: AFR created cone-shaped channels of approximately 300 mu m diameter and 1,850 mu m depth that were Surrounded by a 70 mu m thin layer of thermally coagulated dermis. There was no porphyrin fluorescence in placebo cream or untreated skin sites. AFR followed by MAL application enhanced drug delivery with significantly higher porphyrin fluorescence of hair follicles (P < 0.0011) and dermis (P < 0.0433) versus MAL alone at skin depths of 120, 500, 1,000, 1,500, and 1,800 pro. AFR before MAL application also enhanced skin surface (epidermal) porphyrin fluorescence. Radial diffusion of MAL from the laser-created channels into surrounding dermis was evidenced by uniform porphyrin fluorescence up to 1,500 mu m from the holes (1,000, 1,800 mu m depths). Skin massage after MAL application did not affect MAL-induced porphyrin fluorescence after AFR.Conclusions: Ablative fractional laser treatment facilitates delivery of topical MAL deeply into the skin. for the conditions of this study, laser channels approximately 3 mm apart followed by MAL application could produce porphyrins throughout essentially the entire skin. AFR appears to be a clinically practical means for enhancing uptake of MAL, a photodynamic therapy drug, and presumably many other topical skin medications. Lasers Surg. Med. 42:113-122, 2010. (C) 2009Wiley-Liss, Inc. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:59:11Z |
dc.date.available.fl_str_mv |
2016-01-24T13:59:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Lasers in Surgery and Medicine. Malden: Wiley-Blackwell, v. 42, n. 2, p. 113-122, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/32183 http://dx.doi.org/10.1002/lsm.20860 |
dc.identifier.issn.none.fl_str_mv |
0196-8092 |
dc.identifier.doi.none.fl_str_mv |
10.1002/lsm.20860 |
dc.identifier.wos.none.fl_str_mv |
WOS:000275449600004 |
identifier_str_mv |
Lasers in Surgery and Medicine. Malden: Wiley-Blackwell, v. 42, n. 2, p. 113-122, 2010. 0196-8092 10.1002/lsm.20860 WOS:000275449600004 |
url |
http://repositorio.unifesp.br/handle/11600/32183 http://dx.doi.org/10.1002/lsm.20860 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Lasers in Surgery and Medicine |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
113-122 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764278882631680 |