Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media

Detalhes bibliográficos
Autor(a) principal: Vianna, Lucas Monferrari Monteiro [UNIFESP
Data de Publicação: 2016
Outros Autores: Li, Hao-Dong, Holiman, Jeffrey D., Stoeger, Christopher, Belfort, Rubens Junior [UNIFESP], Jun, Albert S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.5935 / 0004-2.749,20160011
http://repositorio.unifesp.br/handle/11600/49610
Resumo: Purpose: To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). Methods: Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM
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spelling Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented mediaCaracterização de células humanas primárias criopreservados córnea endoteliais cultivadas em meio suplementado com soro humanoCryopreservationCorneaEndotheliumCornealCell Culture TechniquesCulture MediaProliferative CapacityTransplantationPurpose: To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). Methods: Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SMthe other cornea was grown in FBS-SM. On reaching confluence, the six cell populations were frozen using 10% dimethyl sulfoxide-containing medium. Thawed cells grown in HS-SM were compared with those grown in FBS-SM with respect to morphology, growth curves, immunohistochemistry, real time-reverse transcriptase polymerase chain reaction (RT-PCR) for endothelial cell markers, and detachment time. Results: No difference in morphology was observed for cells grown in the two media before or after cryopreservation. By growth curves, cell counts after thawing were similar in both media, with a slight trend toward higher cell counts in FBS-SM. Cells grown in both the media demonstrated a similar expression of endothelial cell markers when assessed by immunohistochemistry, although HCEC marker gene expression was higher in cells grown in HS-SM than in those grown in FBS-SM as assessed by RT-PCR. With FBS-SM, there was a tendency of longer detachment time and lower cell passages. Conclusions: HS-SM was similar to FBS-SM for cryopreservation of cultured HCECs as assessed by analysis of cell morphology, proliferation, and protein expression, although marker gene expression was higher in cells grown in HS-SM than in those grown in FBS-SM. Detachment time was longer with FBS-SM and in lower passages.Cornea and Anterior Segment Service, The Wilmer Eye Institute, Johns Hopkins University, Baltimore (MD), USA|Ophhtalmology and Vision Science Department, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil|Ophthalmology Department, Hospital Universitário Pedro Ernesto, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, RJ, BrazilLions VisionGift, Portland, OR, Estados Unidos da AméricaOphhtalmology and Vision Science Department, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu 821, BR-04023900 Sao Paulo, SP, Brazil.Web of ScienceBrazilian government CAPES FoundationState University of Rio de JaneiroConsel brasil oftalmologia2019-01-21T10:30:09Z2019-01-21T10:30:09Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion37-41application/pdfhttp://dx.doi.org/10.5935 / 0004-2.749,20160011Arquivos Brasileiros De Oftalmologia. Sao paulo, v. 79, n. 1, p. 37-41, 2016.10.5935/0004-2749.20160011WOS000369656700011.pdf0004-2749http://repositorio.unifesp.br/handle/11600/49610WOS:000369656700011engArquivos Brasileiros De Oftalmologiainfo:eu-repo/semantics/openAccessVianna, Lucas Monferrari Monteiro [UNIFESPLi, Hao-DongHoliman, Jeffrey D.Stoeger, ChristopherBelfort, Rubens Junior [UNIFESP]Jun, Albert S.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-10T02:56:08Zoai:repositorio.unifesp.br/:11600/49610Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-10T02:56:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
Caracterização de células humanas primárias criopreservados córnea endoteliais cultivadas em meio suplementado com soro humano
title Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
spellingShingle Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
Vianna, Lucas Monferrari Monteiro [UNIFESP
Cryopreservation
Cornea
Endothelium
Corneal
Cell Culture Techniques
Culture MediaProliferative Capacity
Transplantation
title_short Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
title_full Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
title_fullStr Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
title_full_unstemmed Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
title_sort Characterization of cryopreserved primary human corneal endothelial cells cultured in human serum-supplemented media
author Vianna, Lucas Monferrari Monteiro [UNIFESP
author_facet Vianna, Lucas Monferrari Monteiro [UNIFESP
Li, Hao-Dong
Holiman, Jeffrey D.
Stoeger, Christopher
Belfort, Rubens Junior [UNIFESP]
Jun, Albert S.
author_role author
author2 Li, Hao-Dong
Holiman, Jeffrey D.
Stoeger, Christopher
Belfort, Rubens Junior [UNIFESP]
Jun, Albert S.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vianna, Lucas Monferrari Monteiro [UNIFESP
Li, Hao-Dong
Holiman, Jeffrey D.
Stoeger, Christopher
Belfort, Rubens Junior [UNIFESP]
Jun, Albert S.
dc.subject.por.fl_str_mv Cryopreservation
Cornea
Endothelium
Corneal
Cell Culture Techniques
Culture MediaProliferative Capacity
Transplantation
topic Cryopreservation
Cornea
Endothelium
Corneal
Cell Culture Techniques
Culture MediaProliferative Capacity
Transplantation
description Purpose: To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). Methods: Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM
publishDate 2016
dc.date.none.fl_str_mv 2016
2019-01-21T10:30:09Z
2019-01-21T10:30:09Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935 / 0004-2.749,20160011
Arquivos Brasileiros De Oftalmologia. Sao paulo, v. 79, n. 1, p. 37-41, 2016.
10.5935/0004-2749.20160011
WOS000369656700011.pdf
0004-2749
http://repositorio.unifesp.br/handle/11600/49610
WOS:000369656700011
url http://dx.doi.org/10.5935 / 0004-2.749,20160011
http://repositorio.unifesp.br/handle/11600/49610
identifier_str_mv Arquivos Brasileiros De Oftalmologia. Sao paulo, v. 79, n. 1, p. 37-41, 2016.
10.5935/0004-2749.20160011
WOS000369656700011.pdf
0004-2749
WOS:000369656700011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros De Oftalmologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 37-41
application/pdf
dc.publisher.none.fl_str_mv Consel brasil oftalmologia
publisher.none.fl_str_mv Consel brasil oftalmologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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